| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77179-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92886-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95252-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59376-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12895-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47546SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB93441-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11437 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91920-25ul | |||
![Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0014.jpg "<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20uM of MG132 for 4 h before harvested. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Western Blot: ZEB2 Antibody [NBP1-77179] - Analysis in EL4 cell lysate with antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 ug/ml.Red: ZEB2 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202419333477.gif)
![Western Blot: ZEB2 Antibody [NBP1-77179] - Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, and then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0012.jpg)
![Western Blot: ZEB2 Antibody [NBP1-77179] - The protein levels of EMT-TFs were compared between carcinoma cell lines and non- or less-malignant tumor cell lines by using Western-blot analysis. The soft agar colony transformation or migration assay was referred to distinguish malignant degree of the experimental cancer cell lines (data not shown). Malignant degree: prostate cancer cell lines, P69 < M12; breast cancer cell lines, MCF7 < MD-MBA-231; lung cancer cell lines, A549 < H1299; liver cancer cell lines, HepG2 < SMMC7721. 'kD' (kilo-dalton) means the molecular weight of protein. Image collected and cropped by CiteAb from the following publication (oncotarget.com/fulltext/2825), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/ZEB2-Antibody-Western-Blot-NBP1-77179-img0013.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415392525.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415334920.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202415395947.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 induces degradation of core EMT-TFsA. Immunoblot for core EMT-TFs in 293T cells transfected Zeb1, Zeb2, Snai1, Snai2 or Twist1 with increasing amount of Fbxo45. B. Immunoblot for detecting endogenous core EMT-TFs in HeLa cells transfected with or without myc-tagged Fbxo45, & harvested after treatment with 20μM of MG132 for 4 hours. C. Immunoblot for endogenous Zeb1, Zeb2, Snai1 & Snai2 in MCF7 cells treated with indicated different concentrations of 17 beta -estrogen for 24 h in the phenol-free medium to induce the expression of endogenous Fbxo45. D. Immunoblot for endogenous core EMT-TFs in HeLa transfected with siRNAs for Fbxo45 or Pam. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241535644.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Functional domains of Fbxo45 for ubiquitination of Zeb2A. Lysates from 293T cells transfected Flag-Zeb2 with or without HA-Fbxo45 were immunoprecipitated (IP) with anti-Flag M2 antibody, & then immunoblotted. Total lysates were also used as Input for immunoblotting analysis. B. Bacterially expressed GST or GST–Fbxo45, -Fbxo45 delta F-box, -Fbxo45 delta mid, or -Fbxo45 delta SPRY fusion proteins & Glutathione-Sepharose beads were incubated with lysates of HeLa cells transfected with Zeb2, Snai1 or Snai2, respectively. The proteins associated with GST–tagged Fbxo45 forms, bound on the Glutathione-Sepharose beads were washed five times with the RIPA buffer before immunoblotting. C. Lysates from 293T cells transfected Flag-Zeb2 with HA-Fbxo45, -Fbxo45 delta mid, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were immunoprecipitated with anti-Flag or anti-HA antibodies, & immunoprecipitates were resolved by SDS-PAGE for Western-blot analysis. D. U2OS cells transfected with Flag-Zeb2 & HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY were stained using the primary antibodies of anti-Flag M2 or anti-HA, & the second antibodies of Alexa Fluor 568 anti-mouse or Alexa Fluor 488 anti-Rabbit, respectively. Scale: 25μm. E-I. Zeb2 ubiquitination assays by using IP expriments under different conditions: Flag-Zeb2 with or without HA-Ub (E); Flag-Zeb2 with HA-Ub WT, K48R or K63R (F); Flag-Zeb2 & HA-Ub K48-only with or without myc-Fbox45 (G); Flag-Zeb2 & HA-Ub K48-only with siRNA control, siRNAs for Fbxo45 or siRNAs for Pam (H); Flag-Zeb2 & HA-Ub with HA-Fbxo45, -Fbxo45 delta F-box or -Fbxo45 delta SPRY (I). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416163752.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-310202416175234.jpg)
![Western Blot: ZEB2 Antibody - BSA Free [NBP1-77179] - Fbxo45 is a direct target of miR-27a* that mediates EMT processesA, C. The expression levels of miR-27a* (A) & Fbxo45 mRNA (C) in P69 & M12 were shown according to the data of miRNA transcriptome sequencing & DGE sequencing, respectively [46]. TPM means transcripts per million. B. Real-time PCR in delta delta Ct methods were used to confirm the expression levels of miR-27a* in P69 & M12. One representative experiment out of three was shown. D. Fbxo45 mRNA levels in stable cell lines miR-27a*-overexpressing P69 & miR-27a*-silencing M12 (zipmiR-27a*). P69 or M12 stably infected with the same lentiviral empty vector was used as a control (ctrl). E. Fbxo45 3′-UTR wide-type or mutant form at the position where is complementary to the 5′ seed region of miR-27a* was subcloned into psiCheck2 vector. 293T cells were transfected with psiCheck2 containing WT or mutant Fbxo45 3′-UTR & miR-27a* or non-specific control vector. The Renilla luciferase activity was normalized on the constitutive activity of firefly luciferase. Data are the mean±S.E.M. of three independent experiments. F-G. Immunoblot for Fbxo45, core EMT-TFs (Zeb1, Zeb2, Twist1) & their associated EMT markers (Fibronectin, N-cadherin, Laminin C2, Vimentin & E-cadherin) in stable cell lines P69-miR-27a* or M12-zipmiR-27a* (F); Immunoblot for detection of protein stability of core EMT-TFs under the treatment with 20μM of MG132 for 4 h before harvested (G). GAPDH is for a loading control. H. Immunblot for Fbxo45, N-cadherin, E-cadherin & five core EMT-TFs in stable cell lines PC3-ctrl, PC3-miR-27a* & PC3-zipmiR-27a*. alpha -tubulin is for a loading control. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/25460509), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-31020241616011.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunocytochemistry of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420285893.jpg)
![Immunocytochemistry/ Immunofluorescence: ZEB2 Antibody - BSA Free [NBP1-77179] - Immunofluorescence of ZEB2 in Jurkat cells with ZEB2 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-77179_rabbit-polyclonal-zeb2-antibody-2711202420272496.jpg)
![Western Blot: Aquaporin-4 Antibody [NBP2-92886] - Western blot analysis of extracts of SKOV3 cells, using Aquaporin-4 antibody (NBP2-92886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/Aquaporin-4 Antibody-Western Blot-NBP2-92886-img0003.jpg "Western Blot: Aquaporin-4 Antibody [NBP2-92886] - Western blot analysis of extracts of SKOV3 cells, using Aquaporin-4 antibody (NBP2-92886) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded rat brain using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-92886-img0002.jpg)
![Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded mouse kidney using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Aquaporin-4-Antibody-Immunohistochemistry-Paraffin-NBP2-92886-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody - Azide and BSA Free [NBP2-92886] - Immunofluorescence analysis of paraffin-embedded mouse brain using Aquaporin-4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92886_rabbit-polyclonal-aquaporin-4-antibody-42202514264496.jpg)
![Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody - Azide and BSA Free [NBP2-92886] - Immunofluorescence analysis of paraffin-embedded rat brain using Aquaporin-4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92886_rabbit-polyclonal-aquaporin-4-antibody-4220251430473.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Western-Blot-NBP2-95252-img0008.jpg "Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of various cell lines, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of U2OS cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251639688.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat pancreas using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0007.jpg)
![Western Blot: IRE1 alpha Antibody [NBP2-95252] - Analysis of extracts of 293T cells, using IRE1 alpha antibody (NBP2-95252) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.](http://images.novusbio.com/fullsize/IRE1 alpha Antibody-Western Blot-NBP2-95252-img0009.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Human breast cancer using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0004.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Mouse kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0005.jpg)
![Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NBP2-95252] - Rat kidney using IRE1 alpha Rabbit pAb (NBP2-95252) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/IRE1-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-95252-img0006.jpg)
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of PC-12 cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-52202516334127.jpg)
![Immunocytochemistry/ Immunofluorescence: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunofluorescence analysis of NIH/3T3 cells using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251640267.jpg)
![Immunohistochemistry: IRE1 alpha Antibody - Azide and BSA Free [IRE1 alpha] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IRE1 alpha Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp2-95252_rabbit-polyclonal-ire1-alpha-antibody-5220251633413.jpg)
![Western Blot: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (alpha 2-Macroglobulin) Rabbit mAb (NBP3-16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/alpha-2-Macroglobulin-Antibody-7V6M10-Western-Blot-NBP3-16875-img0007.jpg "Western Blot: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Analysis of extracts of HepG2 cells, using Alpha-2-Macroglobulin (alpha 2-Macroglobulin) Rabbit mAb (NBP3-16875) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Human cervix cancer tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-1110202485424..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Human tonsil tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-1110202485548..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Mouse brain tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-111020248578..jpg)
![Immunohistochemistry-Paraffin: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Rat spleen tissue using Alpha-2-Macroglobulin (A2M) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-immunohistochemistry-paraffin-111020248589..jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded human liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-5220251136127.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded mouse liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511365123.jpg)
![Immunocytochemistry/ Immunofluorescence: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunofluorescence analysis of paraffin-embedded rat liver using Alpha-2-Macroglobulin ) Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511383776.jpg)
![Immunohistochemistry: alpha 2-Macroglobulin Antibody (7V6M10) [NBP3-16875] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Alpha-2-Macroglobulin Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16875_rabbit-alpha-2-macroglobulin-mab-7v6m10-52202511365143.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0005.jpg "Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Western Blot analysis of Rat Liver showing detection of ~180 kDa ATP7A protein using Mouse Anti-ATP7A Monoclonal Antibody, Clone S60-4 (NBP2-59376). Lane 1: MW Ladder. Lane 2: Rat Liver . Load: 10 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse ATP7A Monoclonal Antibody (NBP2-59376) at 1:500 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~180 kDa.")
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody, Clone S60-4 (NBP2-59376). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody (NBP2-59376) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Copper Transporting ATPase 1 Antibody (D) Composite.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0006.jpg)
![Western Blot: ATP7A Antibody (S60-4) [NBP2-59376] - Analysis of Mouse Lung showing detection of >150 kDa Copper Transporting ATPase 1 protein using clone S60-4. 10 ug of sample was loaded and primary antibody was used at 1:1000. Predicted/Observed Size: ~160 kDa. Other band(s): 250 kDa.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Western-Blot-NBP2-59376-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: ATP7A Antibody (S60-4) [NBP2-59376] - Tissue: NIH 3T3 (Mouse Fibroblast cell line). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Copper Transporting ATPase 1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Endoplasmic Reticulum, Cytoplasm, Golgi Apparatus, Trans-Golgi Network Membrane, Cell Membrane. Magnification: 60X.](http://images.novusbio.com/fullsize/ATP7A-Antibody-S60-4-Immunocytochemistry-Immunofluorescence-NBP2-59376-img0002.jpg)
![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0010.jpg "Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Mouse Brain showing detection of ~95 kDa HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Lane 1: MW Ladder. Lane 2: Mouse Brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~95 kDa.")
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) HCN2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0013.jpg)
![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0012.jpg)
![Western Blot: HCN2 Antibody (S71-37) [NBP2-12895] - Western Blot analysis of Rat brain membrane lysate showing detection of HCN2 protein using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Western-Blot-NBP2-12895-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Human hippocampal tissues. Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: HCN2 Antibody (S71-37) [NBP2-12895] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71-37 (NBP2-12895). Tissue: Differentiated SH-SY5Y. Species: Human. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:100. Secondary Antibody: AlexaFluor 488. Counterstain: phalloidin (Alexa 647, red), beta tubulin (Anti-beta III Tubulin Ab, Alexa 555, magenta) Hoechst (blue). (A) Phalloidin (B) Anti-beta III Tubulin Ab. (C) HCN2 Antibody. (D) Hoechst (E) Composite.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunocytochemistry-Immunofluorescence-NBP2-12895-img0014.jpg)
![Immunohistochemistry: HCN2 Antibody (S71-37) [NBP2-12895] - Immunohistochemistry analysis using Mouse Anti-HCN2 Monoclonal Antibody, Clone S71 (NBP2-12895). Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-HCN2 Monoclonal Antibody (NBP2-12895) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT.](http://images.novusbio.com/fullsize/HCN2-Antibody-S71-37-Immunohistochemistry-NBP2-12895-img0011.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Western-Blot-NBP2-67216-img0006.jpg "Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Western blot analysis of Cathepsin L/V/K/H on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate Lane 2: A549 cell lysate")
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0004.jpg)
![Flow Cytometry: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Flow-Cytometry-NBP2-67216-img0001.jpg)
![Western Blot: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1,000 dilution. Positive control: Lane 1: HepG2 Lane 2: A549](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Western-Blot-NBP2-67216-img0005.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded human kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0002.jpg)
![Immunohistochemistry-Paraffin: Cathepsin L/V/K/H Antibody (JM10-78) [NBP2-67216] - Analysis of paraffin-embedded mouse kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Cathepsin-L-V-K-H-Antibody-JM10-78-Immunohistochemistry-Paraffin-NBP2-67216-img0003.jpg)
![Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.](http://images.novusbio.com/fullsize/Calnexin-Antibody-3H4A7-Western-Blot-NBP1-47546-img0002.jpg "Western Blot: Calnexin Antibody (3H4A7) [NBP1-47546] - Western blot analysis using anti-Calnexin monoclonal antibody against A431 (1), Hela (2), MCF-7 (3) and A549 (4) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Calnexin Antibody (3H4A7) [NBP1-47546] - Confocal immunofluorescence analysis of Hela cells using anti-Calnexin mAb (green).](http://images.novusbio.com/fullsize/Calnexin-Antibody-3H4A7-Immunocytochemistry-Immunofluorescence-NBP1-47546-img0003.jpg)
![Western Blot: alpha Tubulin Antibody (961258) [MAB93441] - Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot. Western blot shows lysates of HeLa, Jurkat, NIH-3T3, Neuro2A and Rat-2 cell lines. PVDF membrane was probed with Mouse alpha Tubulin Antibody (961258) (Catalog # MAB93441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at an approximate molecular weight of 50 kDa (as indicated). This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-961258-Western-Blot-MAB93441-img0004.jpg "Western Blot: alpha Tubulin Antibody (961258) [MAB93441] - Detection of Human, Mouse, and Rat alpha Tubulin by Western Blot. Western blot shows lysates of HeLa, Jurkat, NIH-3T3, Neuro2A and Rat-2 cell lines. PVDF membrane was probed with Mouse alpha Tubulin Antibody (961258) (Catalog # MAB93441) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha Tubulin at an approximate molecular weight of 50 kDa (as indicated). This experiment was conducted under reducing conditions.")
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (961258) [MAB93441] - alpha Tubulin was detected in immersion fixed paraffin-embedded sections of human brin hippocampus tissue using alpha Tubulin Antibody (961258) at 5 ug/ml. Tissue was stained using VC001 (brown) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei and cytoplasm in cancer cells.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-961258-Immunohistochemistry-Paraffin-MAB93441-img0002.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody (VEGF/1063) [NBP2-45235] - Human Tonsil stained with VEGF Monoclonal Antibody (VEGF/1063).](http://images.novusbio.com/fullsize/VEGF-Antibody-VEGF-1063-Immunohistochemistry-Paraffin-NBP2-45235-img0003.jpg "Immunohistochemistry-Paraffin: VEGF Antibody (VEGF/1063) [NBP2-45235] - Human Tonsil stained with VEGF Monoclonal Antibody (VEGF/1063).")
![Immunohistochemistry-Paraffin: VEGF Antibody (VEGF/1063) [NBP2-45235] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with VEGF Antibody (VEGF/1063).](http://images.novusbio.com/fullsize/VEGF-Antibody-VEGF-1063-Immunohistochemistry-Paraffin-NBP2-45235-img0006.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody (VEGF/1063) [NBP2-45235] - Formalin-fixed, paraffin-embedded human Tonsil stained with VEGF Antibody (VEGF/1063).](http://images.novusbio.com/fullsize/VEGF-Antibody-VEGF-1063-Immunohistochemistry-Paraffin-NBP2-45235-img0005.jpg)
![Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01] - Cx43, Cx30, and KCNJ10 (Kir4.1) (H00003766-M01) immunoreactivity. Immunoreactivity for Cx43 was maximal at P12 and decayed with age, whereas Cx30 levels increased at later developmental stages. Kir4.1 exhibited a moderately elevated level during the second postnatal week. The last row depicts negative controls at P20, in which the primary antibodies for Cx43 and Cx30 were omitted.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30542265/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-1C11-Immunohistochemistry-H00003766-M01-img0003.jpg "Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01] - Cx43, Cx30, and KCNJ10 (Kir4.1) (H00003766-M01) immunoreactivity. Immunoreactivity for Cx43 was maximal at P12 and decayed with age, whereas Cx30 levels increased at later developmental stages. Kir4.1 exhibited a moderately elevated level during the second postnatal week. The last row depicts negative controls at P20, in which the primary antibodies for Cx43 and Cx30 were omitted.Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30542265/) licensed under a CC-BY license.")
![Sandwich ELISA: KCNJ10 Antibody (1C11) [H00003766-M01] - Detection limit for recombinant GST tagged KCNJ10 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/KCNJ10-Antibody-1C11-Sandwich-ELISA-H00003766-M01-img0002.jpg)
![Immunohistochemistry: KCNJ10 Antibody (1C11) [H00003766-M01] - Cx43, Cx30, & Kir4.1 immunoreactivity. (A) Schematic drawing of an auditory brainstem slice containing the central part of the inferior colliculus (ICC; A1). The tonotopic organization is indicated by dotted lines (A2). The dotted box denotes the area in which Cx expression was analyzed. (B) Immunoreactivity for Cx43 was maximal at P12 & decayed with age, whereas Cx30 levels increased at later developmental stages. Kir4.1 exhibited a moderately elevated level during the second postnatal week. The last row depicts negative controls at P20, in which the primary antibodies for Cx43 & Cx30 were omitted. (C) Developmental profile of Cx43 (C1), Cx30 (C2), & Kir4.1 expression (C3). The dotted gray lines in (C1,2) denote developmental background fluorescence signals at P7–30 obtained after omitting primary antibodies. n represents the number of analyzed slices & is provided within the diagrams; shown are mean values ± SEM. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30542265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00003766-m01_mouse-monoclonal-kcnj10-antibody-1c11-310202415483632.jpg)
![Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.](http://images.novusbio.com/fullsize/GAT3-Antibody-Western-Blot-NBP1-91920-img0006.jpg "Western Blot: GAT3 Antibody [NBP1-91920] - Analysis in human cerebral cortex tissue.")
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebellum shows strong positivity in processes in cells in molecular layer.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0007.jpg)
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0008.jpg)
![Immunohistochemistry-Paraffin: GAT3 Antibody [NBP1-91920] - Staining of human Testis shows moderate cytoplasmic positivity in Leydig cells.](http://images.novusbio.com/fullsize/GAT3-Antibody-Immunohistochemistry-Paraffin-NBP1-91920-img0010.jpg)
