| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09006-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48294 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78376SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB17572-SP | |||
![Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-278202415212422.jpg "Western Blot: NF-L Antibody (PSH0-77) [NBP3-32649] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32649) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32649) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32649, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29820241420992.jpg)
![Immunohistochemistry: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32649) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32649) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-298202414333659.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PSH0-77) [NBP3-32649] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32649) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32649_rabbit-nf-l-mab-psh0-77-29202410234263.jpg)
![Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-278202415212487.jpg "Western Blot: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Western blot analysis of Glutathione Peroxidase 1/GPX1 on different lysates with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: SH-SY5Y cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32389) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32389) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-2982024149266.jpg)
![Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Immunocytochemistry analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1 with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Glutathione Peroxidase 1/GPX1 antibody (NBP3-32389) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-292024119233.jpg)
![Flow Cytometry: Glutathione Peroxidase 1/GPX1 Antibody (C5-A10-R) [NBP3-32389] - Flow cytometric analysis of THP-1 cells labeling Glutathione Peroxidase 1/GPX1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32389, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32389_mouse-glutathione-peroxidase-1-gpx1-mab-c5-a10-r-29202416372194.jpg)
![Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-278202415212420.jpg "Western Blot: NF-L Antibody (PS02-10) [NBP3-32650] - Western blot analysis of NF-L on different lysates with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lane 3: Mouse hippocampus tissue lysate Lane 4: Rat hippocampus tissue lysate Lane 5: Human liver tissue lysate (negative) Lane 6: Mouse liver tissue lysate (negative) Lane 7: Rat liver tissue lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 62 kDa Observed band size: 68 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32650) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-NF-L antibody (NBP3-32650) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32650) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-29820241420994.jpg)
![Immunohistochemistry: NF-L Antibody (PS02-10) [NBP3-32650] - Immunofluorescence analysis of paraffin-embedded mouse brain tissue labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32650, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-298202414262675.jpg)
![Immunocytochemistry/ Immunofluorescence: NF-L Antibody (PS02-10) [NBP3-32650] - Immunocytochemistry analysis of SH-SY5Y cells labeling NF-L with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-NF-L antibody (NBP3-32650) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32650_rabbit-nf-l-mab-ps02-10-2920241192341.jpg)
![Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Analysis of PSMA1 expression in HeLa.](http://images.novusbio.com/fullsize/PSMA1-Antibody-1D9-1C7-Western-Blot-H00005682-M01-img0008.jpg "Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Analysis of PSMA1 expression in HeLa.")
![Immunocytochemistry/Immunofluorescence: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of monoclonal antibody to PSMA1 on HeLa cell. Antibody concentration 60 ug/ml.](http://images.novusbio.com/fullsize/PSMA1-Antibody-1D9-1C7-Immunocytochemistry-Immunofluorescence-H00005682-M01-img0006.jpg)
![Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of PSMA1 over-expressed 293 cell line, cotransfected with PSMA1 Validated Chimera RNAi ( Cat # H00005682-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMA1 monoclonal antibody (M01), clone 1D9-1C7 (Cat # H00005682-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PSMA1-Antibody-1D9-1C7-Western-Blot-H00005682-M01-img0007.jpg)
![Western Blot: PSMA1 Antibody (1D9-1C7) [H00005682-M01] - Analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody (M01), clone 1D9-1C7.Lane 1: PSMA1 transfected lysate(30.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PSMA1-Antibody-1D9-1C7-Western-Blot-H00005682-M01-img0009.jpg)
![Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Western-Blot-NBP3-09006-img0002.jpg "Western Blot: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HeLa (A), HEK293 (B), and HepG2 (C) cell lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of F2C (NBP3-09006) at 0.01 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for alpha Tubulin is ~55kDa. NBP3-09006 successfully detected human alpha Tubulin in HeLa, HEK293, and HepG2 cell lysate samples.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of F2C (NBP3-09006) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing microtubule staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09006, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-09006-img0004.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - Anti-Alpha Tubulin staining of paraffin embedded human cerebral cortex tissue using the rabbit-chimeric version of F2C (NBP3-09006). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP3-09006 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Staining of axons and a few neuronal cell bodies may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Immunohistochemistry-Paraffin-NBP3-09006-img0001.jpg)
![Flow Cytometry: alpha Tubulin Antibody (F2C) - Chimeric [NBP3-09006] - HepG2 (A), HeLa (B) and Hek293 (C) cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of F2C (NBP3-09006, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-F2C-Chimeric-Flow-Cytometry-NBP3-09006-img0003.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0003.jpg "Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Human Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).")
![Immunocytochemistry/Immunofluorescence: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) alpha-Synuclein Antibody. (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunocytochemistry-Immunofluorescence-NBP3-18258-img0001.jpg)
![Immunohistochemistry: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Immunohistochemistry analysis using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Tissue: cerebellum. Species: Rat. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) alpha-Synuclein Antibody (D) Composite.](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Immunohistochemistry-NBP3-18258-img0002.jpg)
![Western Blot: alpha-Synuclein Antibody (3C11) [NBP3-18258] - Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa alpha-Synuclein protein using Mouse Anti-alpha-Synuclein Monoclonal Antibody, Clone 3C11 (NBP3-18258). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-alpha-Synuclein Monoclonal Antibody (NBP3-18258) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).](http://images.novusbio.com/fullsize/alpha-Synuclein-Antibody-3C11-Western-Blot-NBP3-18258-img0004.jpg)
![Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0002.jpg "Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of Hek293 lysates, non-transfected (nt) or transfected (trans) with HA-tagged human Atg9. Arrowhead indicates position of human Atg9 at 105 KDa.")
![Immunocytochemistry/Immunofluorescence: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Staining of PFA fixed HEK293 cells labelled with hamster anti-Atg9 (Left) and rabbit anti-Atg9.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Immunofluorescence-NBP2-50109-img0001.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ATG9A Antibody (Atg9 14F2 8B1) [NBP2-50109] - Analysis of siRNA knockdown of human Atg9 (si) in Hela and Hek293 cells compared to siControl siRNA (siC) reveals specicity of hamster anti-Atg9 antibody.](http://images.novusbio.com/fullsize/ATG9A-Antibody-Atg9-14F2-8B1-Western-Blot-NBP2-50109-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47970-img0001.jpg "Immunohistochemistry-Paraffin: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - Rat Cerebellum stained with Neurofilament Monoclonal Antibody (NFL/736).")
![Flow Cytometry: NF-L Antibody (NFL/736) - Azide and BSA Free [NBP2-47970] - HEK293 cells using Neurofilament Monoclonal Antibody (NFL/736) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/NF-L-Antibody-NFL-736-Azide-and-BSA-Free-Flow-Cytometry-NBP2-47970-img0002.jpg)
![Western Blot: GSAP Antibody [NBP1-78376] - Analysis of GSAP in HepG2 cell lysate.](http://images.novusbio.com/fullsize/GSAP-Antibody-Western-Blot-NBP1-78376-img0002.jpg "Western Blot: GSAP Antibody [NBP1-78376] - Analysis of GSAP in HepG2 cell lysate.")
![Simple Western: GSAP Antibody [NBP1-78376] - Simple Western lane view shows a specific band for GSAP in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GSAP-Antibody-Simple-Western-NBP1-78376-img0003.jpg)
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (972737) [MAB17572] - Antibody was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-972737-Immunohistochemistry-Paraffin-MAB17572-img0002.jpg "Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody (972737) [MAB17572] - Antibody was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue.")
![Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783] - Spectrin beta 3 antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58783-img0002.jpg "Immunocytochemistry/Immunofluorescence: Spectrin beta 3 Antibody [NB110-58783] - Spectrin beta 3 antibody was tested in Neuro 2A cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: Spectrin beta 3 Antibody [NB110-58783] - Detection of Spectrin Beta-III in human hepatocytes using NB110-58783.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunohistochemistry-NB110-58783-img0006.jpg)
![Immunoprecipitation: Spectrin beta 3 Antibody [NB110-58783] - Detection of Human SPTBN2/SCA5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-SPTBN2/SCA5 antibody NB110-58783 used for IP at 3 mcg/mg lysate. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody NB110-58346, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Spectrin-beta-3-Antibody-Immunoprecipitation-NB110-58783-img0005.jpg)