| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74245 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74862 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-86984-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81588-25ul | |||
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0003.jpg "Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.")
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Mouse fore brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Rat hind brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0010.jpg)
![Immunohistochemistry-Whole-Mount: SNAP25 Antibody [NBP1-33714] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: SNAP25 stained by SNAP25 antibody diluted at 1:50.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70 C](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunohistochemistry-whole-mount-127202384126.jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-12720238431..jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-127202384456..jpg)
![SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.Green: SNAP25 protein stained by SNAP25 antibody (NBP1-33714) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunocytochemistry-immunofluorescence-261120251832456.jpg)
![SNAP25 antibody detects SNAP25 protein expression by immunohistochemical analysis.Sample: Frozen sectioned E13.5 Rat brain. Green: SNAP25 protein stained by SNAP25 antibody (NBP1-33714) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunohistochemistry-frozen-261120251829497.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in human cell lines U2OS and MCF-7 using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NBP1-88769-img0017.jpg)
![Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NBP1-88769-img0025.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0020.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0021.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0022.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0023.jpg)
![Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Western-Blot-NBP2-00795-img0002.jpg "Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.")
![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4G5) [NBP2-00795] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunocytochemistry-Immunofluorescence-NBP2-00795-img0009.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human liver tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0008.jpg)
![Flow Cytometry: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Flow-Cytometry-NBP2-00795-img0001.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0003.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0004.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0005.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0007.jpg)
![Western Blot: VAMP-2 Antibody [NBP3-13179] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-2 antibody (NBP3-13179) diluted at 1:1000.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Western-Blot-NBP3-13179-img0001.jpg "Western Blot: VAMP-2 Antibody [NBP3-13179] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with VAMP-2 antibody (NBP3-13179) diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue:Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13179-img0006.jpg)
![Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein expression by immunohistochemical analysis. Sample:Paraffin-Embedded adult mouse retina. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13179-img0007.jpg)
![Immunohistochemistry-Frozen: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: VAMP-2 protein stained by VAMP-2 antibody (NBP3-13179) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Frozen-NBP3-13179-img0002.jpg)
![Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13179-img0003.jpg)
![Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13179-img0004.jpg)
![Immunohistochemistry-Paraffin: VAMP-2 Antibody [NBP3-13179] - VAMP-2 antibody detects VAMP-2 protein at cell membrane in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. VAMP-2 antibody (NBP3-13179) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Immunohistochemistry-Paraffin-NBP3-13179-img0005.jpg)
![Western Blot: VAMP-2 Antibody [NB300-595] - Analysis was performed on tissues (40 ug lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2) and Rat Brain (Lane 3).](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Western-Blot-NB300-595-img0006.jpg "Western Blot: VAMP-2 Antibody [NB300-595] - Analysis was performed on tissues (40 ug lysate) of Mouse Cerebellum (Lane 1), Mouse Brain (Lane 2) and Rat Brain (Lane 3).")
![Flow Cytometry: VAMP-2 Antibody [NB300-595] - Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Flow-Cytometry-NB300-595-img0005.jpg)
![Western Blot: VAMP-2 Antibody [NB300-595] - Analysis of rat brain extract.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-Western-Blot-NB300-595-img0004.jpg)
![Western Blot: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Western blot analysis of extracts of various cell lines, using VAMP-1 Rabbit mAb (NBP3-16212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/VAMP-1-Antibody-1A3K6-Western-Blot-NBP3-16212-img0007.jpg "Western Blot: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Western blot analysis of extracts of various cell lines, using VAMP-1 Rabbit mAb (NBP3-16212) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunofluorescence analysis of U-2 OS cells using VAMP-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-72202512443367.jpg)
![Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded mouse brain tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-7220251244330.jpg)
![Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded mouse colon tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-7220251245355.jpg)
![Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded rat brain tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-72202512435380.jpg)
![Immunohistochemistry: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunohistochemistry analysis of VAMP-1 in paraffin-embedded human brain tissue using VAMP-1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-72202512432096.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunofluorescence analysis of C6 cells using VAMP-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-72202512443348.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-1 Antibody (1A3K6) [NBP3-16212] - Immunofluorescence analysis of NIH-3T3 cells using VAMP-1 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-16212_rabbit-vamp-1-mab-1a3k6-8220252010410.jpg)
![Western Blot: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Western-Blot-NBP2-45413-img0006.jpg "Western Blot: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0005.jpg)
![Western Blot: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY VTI1A.](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Western-Blot-NBP2-45413-img0007.jpg)
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0001.jpg)
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0002.jpg)
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0003.jpg)
![Immunohistochemistry: VTI1A Antibody (1F4) [NBP2-45413] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/VTI1A-Antibody-1F4-Immunohistochemistry-NBP2-45413-img0004.jpg)
![Western Blot: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Western blot analysis of extracts of various cell lines, using VAMP-2 Rabbit mAb (NBP3-16362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-8X9D9-Western-Blot-NBP3-16362-img0003.jpg "Western Blot: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Western blot analysis of extracts of various cell lines, using VAMP-2 Rabbit mAb (NBP3-16362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Immunofluorescence analysis of mouse eye using VAMP-2 Rabbit mAb (NBP3-16362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-8X9D9-Immunohistochemistry-NBP3-16362-img0002.jpg)
![Western Blot: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Western blot analysis of extracts of RD cells, using VAMP-2 Rabbit mAb (NBP3-16362) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-8X9D9-Western-Blot-NBP3-16362-img0004.jpg)
![Immunohistochemistry: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Immunofluorescence analysis of rat eye using VAMP-2 Rabbit mAb (NBP3-16362) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/VAMP-2-Antibody-8X9D9-Immunohistochemistry-NBP3-16362-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Confocal imaging of paraffin-embedded Rat eye using VAMP-2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16362_rabbit-vamp-2-mab-8x9d9-52202511411424.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Confocal imaging of paraffin-embedded Mouse brain using VAMP-2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16362_rabbit-vamp-2-mab-8x9d9-52202511365148.jpg)
![Immunocytochemistry/ Immunofluorescence: VAMP-2 Antibody (8X9D9) [NBP3-16362] - Confocal imaging of paraffin-embedded Mouse eye using VAMP-2 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citRate buffer (pH 6.0) prior to IF staining.](http://images.novusbio.com/fullsize/nbp3-16362_rabbit-vamp-2-mab-8x9d9-52202511383720.jpg)
![Immunocytochemistry/Immunofluorescence: Syntaxin 3 Antibody [NBP1-86984] - Staining of human cell line U-2 OS shows localization to nucleus, nuclear bodies & the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Syntaxin-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-86984-img0016.jpg "Immunocytochemistry/Immunofluorescence: Syntaxin 3 Antibody [NBP1-86984] - Staining of human cell line U-2 OS shows localization to nucleus, nuclear bodies & the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Syntaxin 3 Antibody [NBP1-86984] - Staining of human pancreas shows moderate to strong positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Syntaxin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-86984-img0015.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 3 Antibody [NBP1-86984] - Staining of human placenta shows moderate to strong membranous positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/Syntaxin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-86984-img0012.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 3 Antibody [NBP1-86984] - Staining of human kidney shows moderate to strong positivity in cells in tubules.](http://images.novusbio.com/fullsize/Syntaxin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-86984-img0013.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 3 Antibody [NBP1-86984] - Staining of human duodenum shows moderate to strong positivity in apical membrane in glandular cells and lympohoid cells.](http://images.novusbio.com/fullsize/Syntaxin-3-Antibody-Immunohistochemistry-Paraffin-NBP1-86984-img0014.jpg)
![Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Western-Blot-NBP3-15706-img0004.jpg "Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of Rat brain, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western Blot: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Western blot analysis of extracts of various cell lines, using Syntaxin 1A antibody (NBP3-15706) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Western-Blot-NBP3-15706-img0005.jpg)
![Immunohistochemistry-Paraffin: Syntaxin 1A Antibody (2B2F10) [NBP3-15706] - Immunohistochemistry of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb (NBP3-15706) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Syntaxin-1A-Antibody-2B2F10-Immunohistochemistry-Paraffin-NBP3-15706-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (2B2F10) [Syntaxin 1A] - Immunofluorescence analysis of paraffin-embedded rat brain using Syntaxin 1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15706_rabbit-syntaxin-1a-mab-2b2f10-522025173456.jpg)
![Immunocytochemistry/ Immunofluorescence: Syntaxin 1A Antibody (2B2F10) [Syntaxin 1A] - Immunofluorescence analysis of paraffin-embedded mouse brain using Syntaxin 1A Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-15706_rabbit-syntaxin-1a-mab-2b2f10-522025173352.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding SYT7 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining in human cerebral cortex and skeletal muscle tissues . Corresponding SYT7 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SYT7 Antibody [NBP1-81588] - Analysis in control (vector only transfected HEK293T lysate) and SYT7 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/SYT7-Antibody-Western-Blot-NBP1-81588-img0015.jpg)
![Western Blot: SYT7 Antibody [NBP1-81588] - Analysis in human cell line SK-BR-3.](http://images.novusbio.com/fullsize/SYT7-Antibody-Western-Blot-NBP1-81588-img0009.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in purkinje cells.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0010.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0011.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human prostate shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0012.jpg)
![Immunohistochemistry-Paraffin: SYT7 Antibody [NBP1-81588] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/SYT7-Antibody-Immunohistochemistry-Paraffin-NBP1-81588-img0013.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)