| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88219-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43789 | |||
| BSA Free | NBP2-46641-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43815 | |||
| BSA Free | NBP2-46629-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43788 | |||
| BSA Free | NBP2-46640-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59330-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59326-25ug | |||
![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining in human pancreas and fallopian tube tissues using NBP1-88219 antibody. Corresponding SCGN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP1-88219-img0018.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining in human pancreas and fallopian tube tissues using NBP1-88219 antibody. Corresponding SCGN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry: Secretagogin Antibody [NBP1-88219] - Staining of mouse olfactory bulb, glomerular layer shows nuclear positivity.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-NBP1-88219-img0017.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP1-88219-img0013.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining of human Fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP1-88219-img0014.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining of human pancreas shows strong cytoplasmic and nuclear positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP1-88219-img0015.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP1-88219] - Staining of human rectum shows strong cytoplasmic positivity in peripheral nerve / ganglion.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP1-88219-img0016.jpg)
![Immunohistochemistry: Secretagogin Antibody [NBP1-88219] - Staining of mouse brain shows positivity in fasciola cinereum.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-NBP1-88219-img0011.jpg)
![Immunohistochemistry: Secretagogin Antibody [NBP1-88219] - Staining of mouse hypothalamus shows positivity in the paraventricular hypothalamic nucleus.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-NBP1-88219-img0012.jpg)
![Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2952) [NBP2-46641] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46641 antibody. Corresponding VGLUT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-CL2952-Immunohistochemistry-Paraffin-NBP2-46641-img0015.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2952) [NBP2-46641] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46641 antibody. Corresponding VGLUT2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2952) [NBP2-46641] - Staining of human kidney shows absence of immunoreactivity (negative control).](http://images.novusbio.com/fullsize/VGLUT2-Antibody-CL2952-Immunohistochemistry-Paraffin-NBP2-46641-img0008.jpg)
![Immunohistochemistry: VGLUT2 Antibody (CL2952) [NBP2-46641] - VGLUT2 Antibody (2952) [NBP2-46641] - Staining of rat thalamus shows strong immunoreactivity in glutamatergic synapses.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-CL2952-Immunohistochemistry-NBP2-46641-img0009.jpg)
![Immunohistochemistry: VGLUT2 Antibody (CL2952) [NBP2-46641] - VGLUT2 Antibody (2952) [NBP2-46641] - Staining of mouse olfactory bulb shows strong immunoreactivity in glomeruli.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-CL2952-Immunohistochemistry-NBP2-46641-img0010.jpg)
![Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2952) [NBP2-46641] - Staining of human cerebellum shows strong immunoreactivity in glutamatergic synapses in the molecular and granular layers.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-CL2952-Immunohistochemistry-Paraffin-NBP2-46641-img0011.jpg)
![Immunohistochemistry: VGLUT2 Antibody (CL2952) [NBP2-46641] - VGLUT2 Antibody (2952) [NBP2-46641] - Staining of rat cerebellum shows strong immunoreactivity in glutamatergic fibers in the molecular and granular layers.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-CL2952-Immunohistochemistry-NBP2-46641-img0012.jpg)
![Immunohistochemistry: VGLUT2 Antibody (CL2952) [NBP2-46641] - VGLUT2 Antibody (2952) [NBP2-46641] - Staining of mouse cerebral cortex shows moderate positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-CL2952-Immunohistochemistry-NBP2-46641-img0013.jpg)
![Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2952) [NBP2-46641] - Staining of human cerebral cortex shows moderate immunoreactivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-CL2952-Immunohistochemistry-Paraffin-NBP2-46641-img0014.jpg)
![Western Blot: Secretagogin Antibody [NBP2-20286] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Secretagogin antibody diluted at 1:500.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Western-Blot-NBP2-20286-img0009.jpg "Western Blot: Secretagogin Antibody [NBP2-20286] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Secretagogin antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Secretagogin stained by Secretagogin antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20286-img0008.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0010.jpg)
![Western Blot: Secretagogin Antibody [NBP2-20286] - Sample (30 ug of whole cell lysate) A: HeLa 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Western-Blot-NBP2-20286-img0005.jpg)
![Immunohistochemistry-Paraffin: Secretagogin Antibody [NBP2-20286] - Human colon carcinoma, using SCGN antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Paraffin-NBP2-20286-img0002.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin protein expression by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Secretagogin protein stained by Secretagogin antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:250. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0006.jpg)
![Immunohistochemistry-Frozen: Secretagogin Antibody [NBP2-20286] - Secretagogin antibody detects Secretagogin Protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Secretagogin stained by Secretagogin antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Secretagogin-Antibody-Immunohistochemistry-Frozen-NBP2-20286-img0007.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0010.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Analysis in human cerebral cortex and tonsil tissues using NBP2-46629 antibody. Corresponding GAD2/GAD65 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0009.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse midbrain shows moderate positivity in GABergic neural fibers in the substantia nigra.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0014.jpg)
![Western Blot: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Western-Blot-NBP2-46629-img0008.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat basal forebrain shows moderate immunoreactivity in GABAergic fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0001.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of mouse substantia nigra shows moderate positivity in GABAergic fibers in pars reticulata.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0002.jpg)
![Immunohistochemistry: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of rat globus pallidus shows moderate positivity in GABAergic neural fibers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-NBP2-46629-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebellum shows moderate immunoreactivity in GABAergic fibers in granular and molecular layers.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0005.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human cerebral cortex shows moderate to strong positivity in GABAergic neuronal processes.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0011.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human pancreas shows moderate cytoplasmic positivity in endocrine glandular cells.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0012.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody (CL2736) [NBP2-46629] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-CL2736-Immunohistochemistry-Paraffin-NBP2-46629-img0013.jpg)
![Western Blot: GAD2/GAD65 Antibody [NB110-41528] - Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:4000 antibody2. 1:8000 antibody3. Negative Control](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NB110-41528-img0005.jpg "Western Blot: GAD2/GAD65 Antibody [NB110-41528] - Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit. The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat.Lanes:1. 1:4000 antibody2. 1:8000 antibody3. Negative Control")
![Immunohistochemistry: GAD2/GAD65 Antibody [NB110-41528] - Formalin fixed, paraffin-embedded Rat pancreas tissue sections were stained with 1:1,000 Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit followed by 20 ug/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat and by 20 ug/mL ExtrAvidin-Peroxidase.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-NB110-41528-img0004.jpg)
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NB110-41528] - GAD2/GAD65 labelling in Golden hamster hippocampal fomation. Inset: hilus & dentate gyrus. Image from verified customer review.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NB110-41528-img0003.jpg)
![Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Western-Blot-NBP1-33284-img0012.jpg "Western Blot: GAD2/GAD65 Antibody [NBP1-33284] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GAD65 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GAD2/GAD65 Antibody [NBP1-33284] - RT2 xenograft. GAD65 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunohistochemistry-Paraffin-NBP1-33284-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: GAD2/GAD65 Antibody [NBP1-33284] - Paraformaldehyde-fixed HeLa, using GAD65 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with Red at 1:2500.](http://images.novusbio.com/fullsize/GAD2-GAD65-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33284-img0008.jpg)
![GAD65 antibody detects GAD65 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GAD65 stained by GAD65 antibody (NBP1-33284) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] diluted at 1:500.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp1-33284_rabbit-polyclonal-gad2-gad65-antibody-immunocytochemistry-immunofluorescence-2611202518365910.jpg)
![Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP2-46640] - VGLUT2 Antibody (2921) [NBP2-46640] - Staining in human cerebral cortex and tonsil tissues. Corresponding VGLUT2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-2921-Immunohistochemistry-Paraffin-NBP2-46640-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT2 Antibody (CL2921) [NBP2-46640] - VGLUT2 Antibody (2921) [NBP2-46640] - Staining in human cerebral cortex and tonsil tissues. Corresponding VGLUT2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: VGLUT2 Antibody (2921) [NBP2-46640] - Staining of human cerebellum shows moderate to strong positivity in glutamatergic fibers.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-2921-Immunohistochemistry-Paraffin-NBP2-46640-img0004.jpg)
![Immunohistochemistry: VGLUT2 Antibody (2921) [NBP2-46640] - Staining of mouse brain shows strong positivity in glutamatergic fibers in the thalamus.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-2921-Immunocytochemistry-Immunofluorescence-NBP2-46640-img0005.jpg)
![Immunohistochemistry: VGLUT2 Antibody (2921) [NBP2-46640] - Staining of mouse brain shows positivity in glutamatergic fibers in the cerebellum.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-2921-Immunohistochemistry-Paraffin-NBP2-46640-img0006.jpg)
![Immunohistochemistry: VGLUT2 Antibody (2921) [NBP2-46640] - Staining of rat hippocampus shows moderate positivity in glutamatergic fibers in granular cell layer of the dentate gyrus.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-2921-Immunohistochemistry-Paraffin-NBP2-46640-img0007.jpg)
![Immunohistochemistry: VGLUT2 Antibody (2921) [NBP2-46640] - Staining of rat brain shows moderate to strong positivity in glutamatergic fibers in the cerebellum.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-2921-Immunohistochemistry-Paraffin-NBP2-46640-img0008.jpg)
![Immunohistochemistry-Paraffin: VGLUT2 Antibody (2921) [NBP2-46640] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-2921-Immunohistochemistry-Paraffin-NBP2-46640-img0009.jpg)
![Immunohistochemistry-Paraffin: VGLUT2 Antibody (2921) [NBP2-46640] - Staining of human tonsil shows no positivity in lymphoid cells as expected.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-2921-Immunohistochemistry-Paraffin-NBP2-46640-img0010.jpg)
![Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:000.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Western-Blot-NBP2-59330-img0004.jpg "Western Blot: VGLUT2 Antibody (S29-29) [NBP2-59330] - Western Blot analysis of Rat brain membrane lysate showing detection of VGLUT2 protein using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:000.")
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) VGLUT2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody (S29-29) [NBP2-59330] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VGLUT2 Monoclonal Antibody, Clone S29-29 (NBP2-59330). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-VGLUT2 Monoclonal Antibody (NBP2-59330) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Secretory Vesicle, Synaptic Vesicle Membrane, Membrane, Cell Junction, Synapse. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) VGLUT2 Antibody. (D) Composite.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-S29-29-Immunocytochemistry-Immunofluorescence-NBP2-59330-img0006.jpg)
![Western Blot: Secretagogin Antibody (JE60-48) [NBP3-32933] - Western blot analysis of Secretagogin on different lysates with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse eyeball tissue lysate Lane 4: Mouse pancreas tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32933) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-288202412461645.jpg "Western Blot: Secretagogin Antibody (JE60-48) [NBP3-32933] - Western blot analysis of Secretagogin on different lysates with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. Lane 1: TT cell lysate Lane 2: Human brain tissue lysate Lane 3: Mouse eyeball tissue lysate Lane 4: Mouse pancreas tissue lysate Lane 5: Rat eyeball tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 32 kDa Observed band size: 32 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32933) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Secretagogin Antibody (JE60-48) [NBP3-32933] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32933) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-29820241420927.jpg)
![Immunocytochemistry/ Immunofluorescence: Secretagogin Antibody (JE60-48) [NBP3-32933] - Immunocytochemistry analysis of TT cells labeling Secretagogin with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Secretagogin antibody (NBP3-32933) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-29202410334089.jpg)
![Flow Cytometry: Secretagogin Antibody (JE60-48) [NBP3-32933] - Flow cytometric analysis of TT cells labeling Secretagogin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32933, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32933_rabbit-secretagogin-mab-je60-48-392024884687.jpg)
![Western Blot: VGLUT2 Antibody [NBP3-13164] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT2 antibody (NBP3-13164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-Western-Blot-NBP3-13164-img0001.jpg "Western Blot: VGLUT2 Antibody [NBP3-13164] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VGLUT2 antibody (NBP3-13164) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: VGLUT2 Antibody [NBP3-13164] - VGLUT2 antibody detects VGLUT2 protein by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGLUT2 stained by VGLUT2 antibody (NBP3-13164) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (NBP3-13505) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/VGLUT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13164-img0002.jpg)
![Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Western-Blot-NBP2-59326-img0007.jpg "Western Blot: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Western Blot analysis of Mouse Brain showing detection of ~60 kDa GABA-A-Receptor-Alpha4 protein using Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-GABA-A-Receptor-Alpha4 Monoclonal Antibody (NBP2-59326) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~60 kDa.")
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody, Clone S398A-34 (NBP2-59326). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha 4 Monoclonal Antibody (NBP2-59326) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor Alpha 4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: GABA-AR alpha 4 Antibody (S398A-34) [NBP2-59326] - Analysis using Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody, Clone S398A-34. (Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GABA-A Receptor Alpha4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT.](http://images.novusbio.com/fullsize/GABA-AR-alpha-4-Antibody-S398A-34-Immunocytochemistry-Immunofluorescence-NBP2-59326-img0004.jpg)