| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84830-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70166 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-56011-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58251-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21941-50ul | |||
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Ephrin-B2 Antibody [NBP1-84830] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84830-img0003.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human placenta shows moderate membranous positivity in endothelial cells.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0008.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human colon shows weak to moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0005.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0006.jpg)
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody [NBP1-84830] - Staining of human lung shows weak to moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-Paraffin-NBP1-84830-img0007.jpg)
![Western Blot: Ephrin-B2 Antibody [NBP1-84830] - Colonic levels of EphB4 & ephrin-B2 mRNA. (A) Representative agarose gels showing mRNA levels of EphB4, ephrin-B2 & GAPDH from the colon of vehicle-treated normal mice (N; n = 7) & of TNBS-treated mice administered with vehicle (CNT; n = 4) or EphB4 20 µg/kg (EphB; n = 5). Histograms represent the quantification of EphB4 (B) & ephrin-B2 (C) mRNA levels normalized to GAPDH amplification products. Transcript X1 (black bars) & transcript X2 (white bars), encoded by ephrin-B2 gene, were detected & represented (C). Data are shown as mean ± SEM. *P < 0.05 vs. N mice, one-way ANOVA followed by Bonferroni’s post-test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31297055), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-84830_rabbit-polyclonal-ephrin-b2-antibody-31020241539743.jpg)
![Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Western-Blot-NBP2-46513-img0003.jpg "Western Blot: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY ALOX5 (RC217259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with antiALOX5.(1:2000)")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Adenocarcinoma of Human ovary tissue using antiALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0014.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human colon tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, 1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human Kidney tissue using anti-ALOX5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,(1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Carcinoma of Human kidney tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min (1:150)](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0010.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (3F1) [NBP2-46513] - Paraffin-embedded Human liver tissue using anti-ALOX5 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,1:150).](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-3F1-Immunohistochemistry-Paraffin-NBP2-46513-img0015.jpg)
![Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Raji cells, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Western-Blot-NBP3-16150-img0004.jpg "Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Raji cells, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Immunohistochemistry of paraffin-embedded mouse spleen using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Immunohistochemistry-Paraffin-NBP3-16150-img0002.jpg)
![Western Blot: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Western blot analysis of extracts of Rat lung, using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Western-Blot-NBP3-16150-img0005.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Immunohistochemistry of paraffin-embedded human appendix using 5-Lipoxygenase Rabbit mAb (NBP3-16150) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-5C5Z2-Immunohistochemistry-Paraffin-NBP3-16150-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: 5-Lipoxygenase Antibody (5C5Z2) [NBP3-16150] - Confocal imaging of U-2 OS cells using 5-Lipoxygenase Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16150_rabbit-5-lipoxygenase-mab-5c5z2-5220251242536.jpg)
![Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-56011-img0001.jpg "Immunocytochemistry/Immunofluorescence: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human cell line RT4 shows localization to nucleoplasm. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Analysis in human spleen and skeletal muscle tissues using NBP2-56011 antibody. Corresponding ALOX5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0009.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human spleen shows high expression.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0004.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human lung shows strong nuclear positivity in macrophages.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0006.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0007.jpg)
![Immunohistochemistry-Paraffin: 5-Lipoxygenase Antibody [NBP2-56011] - Staining of human small intestine shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Immunohistochemistry-Paraffin-NBP2-56011-img0008.jpg)
![Western Blot: Ephrin B2 Antibody [NBP1-49857] - (0.1ug/ml) staining of Rat Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Western-Blot-NBP1-49857-img0001.jpg "Western Blot: Ephrin B2 Antibody [NBP1-49857] - (0.1ug/ml) staining of Rat Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry: Ephrin-B2 Antibody [NBP1-49857] - (A) Representative photomicrographs (left panel) and bar graph (right panel) showing AVF venous wall thickness in AVF treated with control or Ephrin-B2/Fc (day 21); *P=0.03 (t-test). n=4. Scale bar, 25um. (B) Line graph showing IVC diameter in mice treated with control or Ephrin-B2/Fc; *P<0.0001 (ANOVA). n=4-5. (C) Bar graphs showing percentage of cells in the AVF positive for proliferation (Ki67) or apoptosis (cleaved caspase-3) (day 21); *P=0.02 (t-test). n=4. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-017-13071-2), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-Immunohistochemistry-NBP1-49857-img0002.jpg)
![Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372] - Western blot analysis of extracts of various cell lines, using Ephrin-B2 Rabbit mAb (NBP3-15372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-1G9E7-Western-Blot-NBP3-15372-img0002.jpg "Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372] - Western blot analysis of extracts of various cell lines, using Ephrin-B2 Rabbit mAb (NBP3-15372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: Ephrin-B2 Antibody (1G9E7) [NBP3-15372] - Immunohistochemistry of paraffin-embedded rat heart using Ephrin-B2 Rabbit mAb (NBP3-15372) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-1G9E7-Immunohistochemistry-Paraffin-NBP3-15372-img0001.jpg)
![Western Blot: Ephrin-B2 Antibody (1G9E7) [NBP3-15372] - Western blot analysis of extracts of various cell lines, using Ephrin-B2 Rabbit mAb (NBP3-15372) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/Ephrin-B2-Antibody-1G9E7-Western-Blot-NBP3-15372-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ephrin-A5 Antibody [NBP2-58251] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Ephrin-A5-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58251-img0001.jpg "Immunocytochemistry/Immunofluorescence: Ephrin-A5 Antibody [NBP2-58251] - Staining of human cell line A-431 shows localization to cytosol & vesicles. Antibody staining is shown in green.")
![Western Blot: 5-Lipoxygenase Antibody (SR2214) [NBP3-21941] - Western blot analysis using NBP3-21941 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-21941_rabbit-5-lipoxygenase-mab-sr2214-5720231523495.jpg "Western Blot: 5-Lipoxygenase Antibody (SR2214) [NBP3-21941] - Western blot analysis using NBP3-21941 in K562 cell lysate.")
![Western Blot: 5-Lipoxygenase Antibody [NB100-53782] - (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/5-Lipoxygenase-Antibody-Western-Blot-NB100-53782-img0003.jpg "Western Blot: 5-Lipoxygenase Antibody [NB100-53782] - (0.3ug/ml) staining of Rat Stomach lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr317 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.](http://images.novusbio.com/fullsize/EPH-Receptor-B1-[p-Tyr317]-Antibody-Western-Blot-NB300-275-img0001.jpg "Western Blot: Ephrin-B1 [p Tyr317] Antibody [NB300-275] - Rat testes lysate showing specific immunolabeling of the ~46k EphriNBphosphorylated at Tyr317 (control, lane 1). The phosphospecificity of this labeling is shown in lane 2 (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-phosphatase (1200 units for 30 min) before being exposed to the Anti-Tyr317 EphrinB. The immunolabeling of the EphriNBis completely eliminated by treatment with lambda-phosphatase.")
![Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01] - EFNA5 monoclonal antibody (M01), clone 1F12. Analysis of EFNA5 expression in IMR-32.](http://images.novusbio.com/fullsize/Ephrin-A5-Antibody-1F12-Western-Blot-H00001946-M01-img0005.jpg "Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01] - EFNA5 monoclonal antibody (M01), clone 1F12. Analysis of EFNA5 expression in IMR-32.")
![Western Blot: Ephrin-A5 Antibody (1F12) [H00001946-M01] - analysis of EFNA5 expression in NIH/3T3 ( Cat # L018V1 ).](http://images.novusbio.com/fullsize/Ephrin-A5-Antibody-1F12-Western-Blot-H00001946-M01-img0001.jpg)
![ELISA: Ephrin-A5 Antibody (1F12) [H00001946-M01] - Detection limit for recombinant GST tagged EFNA5 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Ephrin-A5-Antibody-1F12-ELISA-H00001946-M01-img0006.jpg)
![Western Blot: Ephrin-B Antibody [NBP1-59211] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize/Ephrin-B-Antibody-Western-Blot-NBP1-59211-img0003.jpg "Western Blot: Ephrin-B Antibody [NBP1-59211] - Sample Tissue: Human Fetal Liver Antibody Dilution: 1.0 ug/ml")
![Western Blot: Ephrin-B Antibody [NBP1-59211] - Human kidney lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/Ephrin-B-Antibody-Western-Blot-NBP1-59211-img0002.jpg)