| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55289SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47965-0.1mg | |||
| IHC-Prediluted | NBP2-48295 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44831 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59779 | |||
| BSA Free | NBP2-59779-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05555-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05551-50ul | |||
![Western Blot: LRRK2 Antibody [NB110-55289] - Detection of murine LRRK2 in mouse brain lysate.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Western-Blot-NB110-55289-img0006.jpg "Western Blot: LRRK2 Antibody [NB110-55289] - Detection of murine LRRK2 in mouse brain lysate.")
![Immunohistochemistry: LRRK2 Antibody [NB110-55289] - Staining of neurons and glia. Mouse Brain, 40X.](http://images.novusbio.com/fullsize/LRRK2-Antibody-Immunohistochemistry-NB110-55289-img0007.jpg)
![Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Western-Blot-NB600-879-img0005.jpg "Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.")
![Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879] - Diluted 1:500 to detect Notch-2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/Notch-2-Antibody-Immunohistochemistry-Paraffin-NB600-879-img0003.jpg)
![Western Blot: Zic2 Antibody [NBP2-24607] - Analysis of ZIC2 in HepG2 cell lysate using 1) pre-bleed, and 2) this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Zic2-Antibody-Western-Blot-NBP2-24607-img0001.jpg "Western Blot: Zic2 Antibody [NBP2-24607] - Analysis of ZIC2 in HepG2 cell lysate using 1) pre-bleed, and 2) this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: PSENEN/PEN2 Antibody [NBP2-24611] - Analysis of Presenilin enhancer protein 2 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse intestine lysate using NBP2-24611. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/PSENEN-PEN2-Antibody-Western-Blot-NBP2-24611-img0002.jpg "Western Blot: PSENEN/PEN2 Antibody [NBP2-24611] - Analysis of Presenilin enhancer protein 2 in human intestine lysate in the 1) absence and 2) presence of immunizing peptide and 3) mouse intestine lysate using NBP2-24611. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - LSM image showing strong expression of 5-HT6 (red) colocalized with virally transduced (green) TH (blue)-positive neurons within the dopaminergic fetal graft. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-NBP1-46557-img0002.jpg "Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - LSM image showing strong expression of 5-HT6 (red) colocalized with virally transduced (green) TH (blue)-positive neurons within the dopaminergic fetal graft. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.")
![Immunohistochemistry: 5-HT6 Antibody [NBP1-46557] - The 5-HT6 Receptor Is highly expressed in human tissue grafts originating from both fetal and hESC. Immunofluorescence staining of 5-HT6 expression in a human dopaminergic fetal graft within the rat striatum with the phenotypic staining for TH , and the identification of human NCAM, imaged using LSM. Image collected and cropped by CiteAb from the following publication (linkinghub.elsevier.com/retrieve/pii/S0896627316300769), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/5-HT6-Antibody-Immunohistochemistry-NBP1-46557-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: 5-HT6 Antibody [NBP1-46557] - The 5-HT6 Receptor Is Highly Expressed in Human Tissue Grafts Originating from Both Fetal & hESC Sources(A–E) Immunofluorescence staining of 5-HT6 expression in a human dopaminergic fetal graft within the rat striatum (A & C) with the phenotypic staining for TH (A & B), & the identification of human NCAM (A & D), imaged using LSM. The 5-HT6 expression was confirmed to reside in DA neurons originating from the transplanted tissue (E).(F–J) Similarly, immunofluorescence staining of a paraffin-embedded section originating from the putamen of a PD patient that received a dopaminergic fetal graft, imaged using LSM, confirmed that the 5-HT6 receptor (F & H) is highly expressed in the TH-positive (F & G, with overlay in J) DA neurons originating from the fetal graft. Nuclear staining (DAPI) confirmed the 5-HT6 expression to be abundant in the neuronal soma (I).(K–O) To confirm if this receptor is also abundant in DA neurons differentiated from hESCs, a graft derived from the H9 hESC line, differentiated using the novel floor-plate-based protocol & transplanted to the parkinsonian striatum of a nude rat, was evaluated using LSM with immunofluorescence for the same genes: 5-HT6 (K & M), TH (K & L), & the human NCAM (K & N). This confirmed that this receptor is highly expressed in DA neurons also differentiated from this cell source, when transplanted to the striatum. Overlay of all three antigens confirmed the 5-HT6 receptor to reside in hESC-derived DA neurons (O). Scale bar, 100 μm (K) & 50 μm (N). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27161524), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-46557_rabbit-polyclonal-5-ht6-antibody-310202416171411.jpg)
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [NBP2-44874] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with NGFR/TNFRSF16/p75NTR Antibody (NGFR5).](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-NGFR5-Immunohistochemistry-Paraffin-NBP2-44874-img0006.jpg "Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [NBP2-44874] - Formalin-fixed, paraffin-embedded human Adrenal Gland stained with NGFR/TNFRSF16/p75NTR Antibody (NGFR5).")
![Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (NGFR5) [NBP2-44874] - Formalin-fixed, paraffin-embedded human Melanoma stained with NGFR/TNFRSF16/p75NTR Antibody (NGFR5).](http://images.novusbio.com/fullsize/NGFR-TNFRSF16-p75NTR-Antibody-NGFR5-Immunohistochemistry-Paraffin-NBP2-44874-img0005.jpg)
![Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Rat Retina lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Western-Blot-NBP1-51987-img0001.jpg "Western Blot: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Rat Retina lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Sodium Potassium ATPase Beta 1 Antibody [NBP1-51987] - Staining of Human Small Intestine. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Sodium-Potassium-ATPase-Beta-1-Antibody-Immunohistochemistry-Paraffin-NBP1-51987-img0003.jpg)
![Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-278202416285276.jpg "Western Blot: Cofilin Antibody (PSH01-44) [NBP3-32207] - Western blot analysis of Cofilin on different lysates with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/1,000 dilution. Lane 1: Hela cell lysate (30 ug/Lane) Lane 2: HEK-293 cell lysate (30 ug/Lane) Lane 3: MCF7 cell lysate (30 ug/Lane) Lane 4: MDA-MB-468 cell lysate (30 ug/Lane) Lane 5: SH-SY5Y cell lysate (30 ug/Lane) Lane 6: HUVEC cell lysate (30 ug/Lane) Lane 7: Jurkat cell lysate (30 ug/Lane) Lane 8: COS-1 cell lysate (30 ug/Lane) Lane 9: VERO cell lysate (30 ug/Lane) Lane 10: NIH/3T3 cell lysate (30 ug/Lane) Lane 11: PC-12 cell lysate (30 ug/Lane) Lane 12: Neuro-2a cell lysate (30 ug/Lane) Lane 13: K-562 cell lysate (30 ug/Lane) Lane 14: A431 cell lysate (30 ug/Lane) Predicted band size: 19 kDa Observed band size: 19 kDa Exposure time: 2 minutes 37 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32207) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32207) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2982024142099.jpg)
![Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (PSH01-44) [NBP3-32207] - Immunocytochemistry analysis of HeLa cells labeling Cofilin with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Cofilin antibody (NBP3-32207) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-2920241048483.jpg)
![Flow Cytometry: Cofilin Antibody (PSH01-44) [NBP3-32207] - Flow cytometric analysis of HeLa cells labeling Cofilin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32207, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32207_rabbit-cofilin-mab-psh01-44-29202416372168.jpg)
![Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Western-Blot-NBP2-34809-img0001.jpg "Western Blot: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Western Blot Analysis of human A375 cell lysate using Vimentin Antibody (SPM576).")
![Immunohistochemistry-Paraffin: Vimentin Antibody (SPM576) - Azide and BSA Free [NBP2-34809] - Formalin-paraffin human melanoma stained with Vimentin MAb (SPM576).](http://images.novusbio.com/fullsize/Vimentin-Antibody-SPM576-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34809-img0002.jpg)
![Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Western-Blot-NBP3-05555-img0002.jpg "Western Blot: Tyrosine Hydroxylase Antibody [NBP3-05555] - Western blot analysis of different tissue lysates using Tyrosine Hydroxylase Antibody at a dilution of 1:50,000 in green: [1] protein standard (red), [2] rat brain caudate/putmen region, [3] rat brain midbrain, [4] whole mouse brain and brain stem excluding cerebellum, and [5] segment of cow midbrain. Strong band at about 58kDa corresponds to the Tyrosine Hydroxylase protein.")
![Immunocytochemistry/Immunofluorescence: Tyrosine Hydroxylase Antibody [NBP3-05555] - Immunofluorescent analysis of rat brain section stained with Tyrosine Hydroxylase Antibody at a dilution of 1:5,000, in green. The blue is Hoechst staining of nuclear DNA. Tyrosine Hydroxylase Antibody stains the dopaminergic TH-positive neurons and their processes of the substantia nigra.](http://images.novusbio.com/fullsize/Tyrosine-Hydroxylase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05555-img0001.jpg)
![Western Blot: CNPase Antibody [NBP3-05551] - Western blot analysis of different tissue lysates using goat pAb to CNPase at a dilution of 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNPase protein.](http://images.novusbio.com/fullsize/CNPase-Antibody-Western-Blot-NBP3-05551-img0002.jpg "Western Blot: CNPase Antibody [NBP3-05551] - Western blot analysis of different tissue lysates using goat pAb to CNPase at a dilution of 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNPase protein.")
![Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP3-05551] - Immunofluorescent analysis of a section of mouse striatum stained with CNPase Antibody at a dilution of 1:3,000 in green, and costained with rabbit pAb to NF-L at a dilution of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNPase antibody stains myelin sheath and the plasma membranes of oligodendrocytes, the myelin producing cells of the CNS. The NF-L antibody labels axons of neuronal cells enclosed by the myelin.](http://images.novusbio.com/fullsize/CNPase-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05551-img0001.jpg)