| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47611-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22405-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37525-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-37713 | |||
![Western Blot: NMDA R, NR2C Subunit Antibody [NB300-107]](http://images.novusbio.com/fullsize/NMDA-R--NR2C-Subunit-Antibody-Western-Blot-NB300-107-img0005.jpg "Western Blot: NMDA R, NR2C Subunit Antibody [NB300-107]")
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0008.jpg "Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NMDAR2B protein using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NMDAR2B Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0010.jpg)
![Immunohistochemistry: NMDAR2B Antibody (S59) [NBP1-47611] - Immunohistochemistry analysis using Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody, Clone S59-36 (NBP1-47611). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NMDAR2B Monoclonal Antibody (NBP1-47611) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunohistochemistry-NBP1-47611-img0009.jpg)
![Western Blot: NMDAR2B Antibody (S59) [NBP1-47611] - analysis of Human HEK-T lysates showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36 . Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Western-Blot-NBP1-47611-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody (S59) [NBP1-47611] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.](http://images.novusbio.com/fullsize/NMDAR2B-Antibody-S59-Immunocytochemistry-Immunofluorescence-NBP1-47611-img0005.jpg)
![Western Blot: NMDAR2A Antibody (S327-95) [NBP2-22405] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-NMDAR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22405) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327-95-Western-Blot-NBP2-22405-img0003.jpg "Western Blot: NMDAR2A Antibody (S327-95) [NBP2-22405] - Western Blot analysis of Monkey COS cells transfected with GFP-tagged NMDAR2A showing detection of ~170 kDa NMDAR2A protein using Mouse Anti-NMDAR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Lane 1: Molecular Weight Ladder. Lane 2: Monkey COS cells transfected with GFP-tagged NMDAR2A. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-NMDAR2A Monoclonal Antibody (NBP2-22405) at 1:200 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~170 kDa. Other Band(s): 100 kDa.")
![Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (NBP2-22405) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2A/NR2A Antibody (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327-95-Immunocytochemistry-Immunofluorescence-NBP2-22405-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327-95-Immunocytochemistry-Immunofluorescence-NBP2-22405-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NMDAR2A Antibody (S327-95) [NBP2-22405] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2A/NR2A Monoclonal Antibody, Clone S327-95 (NBP2-22405). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-GluN2A/NR2A Monoclonal Antibody (NBP2-22405) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Cell Junction. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GluN2A/NR2A Antibody. (D) Composite.](http://images.novusbio.com/fullsize/NMDAR2A-Antibody-S327-95-Immunocytochemistry-Immunofluorescence-NBP2-22405-img0005.jpg)
![Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Western-Blot-NBP2-37525-img0002.jpg "Western Blot: NF-L Antibody (2G10) [NBP2-37525] - Analysis using NEFL mouse mAb against Hela (1) and Jurkat (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Hela cells using NEFL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunocytochemistry-Immunofluorescence-NBP2-37525-img0003.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of brain tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0006.jpg)
![Flow Cytometry: NF-L Antibody (2G10) [NBP2-37525] - Analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Flow-Cytometry-NBP2-37525-img0004.jpg)
![Immunohistochemistry-Paraffin: NF-L Antibody (2G10) [NBP2-37525] - Analysis of lung cancer tissues using NEFL mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-Immunohistochemistry-NBP2-37525-img0005.jpg)
![ELISA: NF-L Antibody (2G10) [NBP2-37525] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/NF-L-Antibody-2G10-ELISA-NBP2-37525-img0001.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0006.jpg "Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)")
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0001.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0002.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0003.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0004.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0005.jpg)
![Immunohistochemistry: Ephrin-A3 Antibody (OTI4A4) - Azide and BSA Free [NBP2-70623] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Ephrin-A3-Antibody-OTI4A4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70623-img0007.jpg)
![Immunohistochemistry-Paraffin: Mouse Ephrin-A3 mAb (OTI4A4) - Azide and BSA Free [NBP2-70623] - Ephrin-A3 immunoreactivity in mouse trigeminal ganglion neurons. Sections were incubated with the antibody overnight at 4 degrees. Biotinylated mouse IgG was used to help verify the specificity of the antibody (NBP1-97078). Biotin conjugated version of antibody used (NBP2-70623B). Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-70623_mouse-ephrin-a3-mab-oti4a4-azide-and-bsa-free-immunohistochemistry-paraffin-2842023144245.jpg)
![Western Blot: Slug Antibody (OTI2B11) - Azide and BSA Free [NBP2-74235] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Slug.](http://images.novusbio.com/fullsize/Slug-Antibody-OTI2B11-Azide-and-BSA-Free-Western-Blot-NBP2-74235-img0001.jpg "Western Blot: Slug Antibody (OTI2B11) - Azide and BSA Free [NBP2-74235] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Slug.")
![Immunocytochemistry/Immunofluorescence: Slug Antibody (OTI2B11) - Azide and BSA Free [NBP2-74235] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Slug.](http://images.novusbio.com/fullsize/Slug-Antibody-OTI2B11-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-74235-img0005.jpg)
![Immunohistochemistry: Slug Antibody (OTI2B11) - Azide and BSA Free [NBP2-74235] - Analysis of Human tonsil tissue.](http://images.novusbio.com/fullsize/Slug-Antibody-OTI2B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74235-img0004.jpg)
![Flow Cytometry: Slug Antibody (OTI2B11) - Azide and BSA Free [NBP2-74235] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by SNAI2 antibody.](http://images.novusbio.com/fullsize/Slug-Antibody-OTI2B11-Azide-and-BSA-Free-Flow-Cytometry-NBP2-74235-img0002.jpg)
![Immunohistochemistry: Slug Antibody (OTI2B11) - Azide and BSA Free [NBP2-74235] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/Slug-Antibody-OTI2B11-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74235-img0003.jpg)
![Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-288202414191539.jpg "Western Blot: Vimentin Antibody (PDH0-01) [NBP3-33093] - Western blot analysis of Vimentin on different lysates with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/5,000 dilution. Lane 1: L6 cell lysate Lane 2: C2C12 cell lysate Lane 3: HeLa cell lysate Lane 4: A549 cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 54 kDa Observed band size: 57 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33093) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33093) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-29820241433367.jpg)
![Immunohistochemistry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33093, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-298202414304144.jpg)
![Immunocytochemistry/ Immunofluorescence: Vimentin Antibody (PDH0-01) [NBP3-33093] - Immunocytochemistry analysis of HeLa cells labeling Vimentin with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Vimentin antibody (NBP3-33093) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-2920241114843.jpg)
![Flow Cytometry: Vimentin Antibody (PDH0-01) [NBP3-33093] - Flow cytometric analysis of Hela cells labeling Vimentin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33093, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-33093_rabbit-vimentin-mab-pdh0-01-392024884612.jpg)
![Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Western-Blot-NBP2-75531-img0006.jpg "Western Blot: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of Huntingtin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysateLane 2: A431 cell lysate")
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded rat brain tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0005.jpg)
![Flow Cytometry: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of SH-SY-5Y cells with Huntingtin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Flow-Cytometry-NBP2-75531-img0001.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human colon cancer tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0002.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded human breast tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0003.jpg)
![Immunohistochemistry-Paraffin: Huntingtin Antibody (JB89-34) [NBP2-75531] - Analysis of paraffin-embedded mouse epididymis tissue using anti-Huntingtin antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Huntingtin-Antibody-JB89-34-Immunohistochemistry-Paraffin-NBP2-75531-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Analysis using the Alexa Fluor (R) 647 conjugate of NB500-523. Staining of CD16a in human buffy coat using anti-CD16a antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Ima](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-MEM-168-Flow-Cytometry-NB500-523-img0005.jpg "Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Analysis using the Alexa Fluor (R) 647 conjugate of NB500-523. Staining of CD16a in human buffy coat using anti-CD16a antibody. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Ima")
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Surface staining of lysed and washed porcine peripheral blood with purified anti-CD16 (MEM-168) (detection by anti-mouse IgM FITC). Panel A - porcine PBMC stained with Isotype mouse IgM control (PFR-03) Panel B, C, D - three different porcine PMBC samples stained with anti-CD16 (MEM-168) Cells in the granulocyte gate were used for analysis.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-MEM-168-Flow-Cytometry-NB500-523-img0001.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Analysis using the Alexa Fluor (R) 488 conjugate of NB500-523. Staining of CD16a in human buffy coat using anti-CD16a antibody AF488. The primary antibody was used at a dilution of 1:100 and incubated for 25 min at 4C in 2% human serum, 0.5 mM EDTA in DPBS. Image from verified customer review.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-MEM-168-Flow-Cytometry-NB500-523-img0004.jpg)
![Flow Cytometry: Fc gamma RIIIA/CD16a Antibody (MEM-168) [NB500-523] - Human PBMC stained with Fc gamma RIIIA/CD16a antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/Fc-gamma-RIIIA-CD16a-Antibody-MEM-168-Flow-Cytometry-NB500-523-img0007.jpg)
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).](http://images.novusbio.com/fullsize/Fc-gamma-RIII-CD16-Antibody-MEM-154-Flow-Cytometry-NB500-378-img0002.jpg "Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Separation of FasL transfected L5178Y cells stained using anti-human CD178 (NOK-1) purified antibody (concentration in sample 9 ug/ml, GAM APC, red-filled) from FasL transfected L5178Y cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).")
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) [NB500-378] - Flow cytometry surface staining pattern of human peripheral blood stained using Fc gamma RIII (CD16) Antibody (MEM-154) purified antibody (concentration in sample 2 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/Fc-gamma-RIII-CD16-Antibody-MEM-154-Flow-Cytometry-NB500-378-img0001.jpg)
![Flow Cytometry: Fc gamma RIII (CD16) Antibody (MEM-154) - BSA Free [NB500-378] - Staining of human peripheral blood cells with anti-human CD16 (MEM-154) purified, GAM/APC.](http://images.novusbio.com/fullsize/nb500-378_mouse-monoclonal-fc-gamma-riii-cd16-antibody-mem-154-17720241003218.jpg)
![Western Blot: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 expression in transfected 293T cell line by DLX4 monoclonal antibody (M01), clone 1F11.Lane 1: DLX4 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/BP1-Antibody-1F11-Western-Blot-H00001748-M01-img0008.jpg "Western Blot: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 expression in transfected 293T cell line by DLX4 monoclonal antibody (M01), clone 1F11.Lane 1: DLX4 transfected lysate(26 KDa).Lane 2: Non-transfected lysate.")
![<b>Genetic Strategies Validation. </b>Western Blot: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 over-expressed 293 cell line, cotransfected with DLX4 Validated Chimera RNAi ( Cat # H00001748-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DLX4 monoclonal antibody (M01), clone 1F11 (Cat # H00001748-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/BP1-Antibody-1F11-Western-Blot-H00001748-M01-img0006.jpg)
![Immunoprecipitation: BP1 Antibody (1F11) [H00001748-M01] - Analysis of DLX4 transfected lysate using anti-DLX4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DLX4 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/BP1-Antibody-1F11-Immunoprecipitation-H00001748-M01-img0005.jpg)
![ELISA: BP1 Antibody (1F11) [H00001748-M01] - Detection limit for recombinant GST tagged DLX4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/BP1-Antibody-1F11-ELISA-H00001748-M01-img0009.jpg)