| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56437SS | |||
| BSA Free | NB100-56437 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-132SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-676SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77067SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-78292SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87842-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NBP2-45225AFP488 | |||
| Alexa Fluor Plus 555 | NBP2-45225AFP555 | |||
| Alexa Fluor Plus 594 | NBP2-45225AFP594 | |||
| Alexa Fluor Plus 647 | NBP2-45225AFP647 | |||
| Alexa Fluor Plus 680 | NBP2-45225AFP680 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-675SS | |||
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Western blot shows lysates of Jurkat human T lymphocyte parental cell line and CXCR4 knockout (KO) Jurkat cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human CXCR4 Polyclonal Antibody (Catalog # NB100-56437) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for CXCR4 at approximately 38 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in the knockout Jurkat cell line. This experiment was conducted under reducing conditions. Image using the standard format of this product.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CXCR4 Antibody - Azide Free [NBP2-24862] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-24862-img0002.jpg)
![Immunohistochemistry: CXCR4 Antibody - Azide Free [NBP2-24862] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue). Image using the standard format of this product.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Immunohistochemistry-NBP2-24862-img0003.jpg)
![Western Blot: CXCR4 Antibody - Azide Free [NBP2-24862] - Analysis of 15 ug of whole cell lysate from HeLa cells with anti-CXCR4 at 1 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody---Azide-Free-Western-Blot-NBP2-24862-img0001.jpg)
![Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Western-Blot-NB400-149-img0003.jpg "Western Blot: PDZK1 Antibody [NB400-149] - WB analysis of PDZK1 in fetal human liver lysate.")
![Immunocytochemistry/Immunofluorescence: PDZK1 Antibody [NB400-149] - PDZK1 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunocytochemistry-Immunofluorescence-NB400-149-img0004.jpg)
![Immunohistochemistry: PDZK1 Antibody [NB400-149] - IHC analysis of PDZK1 in mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Immunohistochemistry-NB400-149-img0005.jpg)
![Simple Western: PDZK1 Antibody [NB400-149] - Simple Western lane view shows a specific band for PDZK1 in 0.2 mg/ml of Human Liver lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.](http://images.novusbio.com/fullsize/PDZK1-Antibody-Simple-Western-NB400-149-img0006.jpg)
![Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Western-Blot-NB300-132-img0003.jpg "Western Blot: NF-L Antibody (DA2) [NB300-132] - Rat spinal cord homogenate showing the major intermediate filament proteins of the nervous system (lane 1). The remaining lanes show blots of this material stained with various antibodies including NB300-132 (lane 4).")
![Immunocytochemistry/Immunofluorescence: NF-L Antibody (DA2) [NB300-132] - Staining of neurons in culture using NF-L monoclonal DA2 antibody (green) imaged with a 40X lens. Nuclei are stained blue. Photo Courtesy of Dr. Gerry Shaw.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Immunocytochemistry-Immunofluorescence-NB300-132-img0004.jpg)
![Simple Western: NF-L Antibody (DA2) [NB300-132] - Image shows a specific band for NF-L in 0.5 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/NF-L-Antibody-DA2-Simple-Western-NB300-132-img0005.jpg)
![Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Knockout-Validated-NB300-676-img0013.jpg "<b>Genetic Strategies Validation. </b>Knockout Validated: UCH-L1/PGP9.5 Antibody [NB300-676] - Western blot shows lysates of HEK293T human embryonic kidney parental cell line and UCH-L1/PGP9.5 knockout (KO) HEK293T cell line. PVDF membrane was probed with 2 ug/ml of Rabbit Anti-Human UCH-L1/PGP9.5 Polyclonal Antibody (Catalog # NB300-676) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for UCH-L1/PGP9.5 at approximately 28 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in the knockout HEK293T cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: UCH-L1/PGP9.5 Antibody [NB300-676] - Analysis of UCHL1 in mouse brain lysate ECL detection, 60 seconds.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-676-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-676] - Neuro2a cells stained with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-676-img0007.jpg)
![Immunohistochemistry-Paraffin: UCH-L1/PGP9.5 Antibody [NB300-676] - Staining of neurons and cell processes at 2.5ug/mL. Human brain, Substantia Nigra, Pigmented Neurons, 4X.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-Paraffin-NB300-676-img0009.jpg)
![Flow Cytometry: UCH-L1/PGP9.5 Antibody [NB300-676] - An intracellular stain was performed on U-87 cells with UCH-L1/PGP9.5 Antibody NB300-676AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/UCH-L1-PGP9.5-Antibody-Flow-Cytometry-NB300-676-img0012.jpg)
![Simple Western: UCH-L1/PGP9.5 Antibody [NB300-676] - Image shows a specific band for PGP9.5/UCHL-1 in 0.05 mg/mL of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Simple-Western-NB300-676-img0011.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0013.jpg "Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: 15 ug of lysates per lane. Antibodies: NBP1-7706 (1 ug/mL), NBP1-76480 (1 ug/mL), and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP")
![Immunohistochemistry: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in Human Spleen. Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody at 5 u/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420324853.gif)
![<b>Genetic Strategies Validation. </b>Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITClabeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4-/- astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0010.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa whole cell lysate with CXCR4 antibody at 0.5 ug/ml.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0001.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Recombinant Protein Test: Loading: CXCR4 partial recombinant protein (H00007852-Q01). Lane 1: Anti-CXCR4 antibody (0.1 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0007.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0008.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0009.jpg)
![Western Blot: CXCR4 Antibody [NBP1-77067] - Loading: Lysates/proteins at 20 ug per lane. Antibodies: NBP1-7706 (2 ug/mL) or NBP1-76480 (2 ug/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0011.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: CXCR4 Antibody [NBP1-77067] - HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-7706 (2 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Western-Blot-NBP1-77067-img0012.jpg)
![Flow Cytometry: CXCR4 Antibody [NBP1-77067] - Analysis of HeLa cells using CXCR4 antibody at 0.1 ug/ml. Purple: cells without staining, Green: Isotype control. Red : CXCR4 antibody.](http://images.novusbio.com/fullsize/CXCR4-Antibody-Flow-Cytometry-NBP1-77067-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody at 2 u/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4;C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202419313214.gif)
![Immunocytochemistry/ Immunofluorescence: CXCR4 Antibody - BSA Free [NBP1-77067] - Validation of CXCR4 in HeLa Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.](http://images.novusbio.com/fullsize/nbp1-77067_rabbit-polyclonal-cxcr4-antibody-2711202420414588.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat brain lysate showing immunolabeling of the ~45-47 kDa GABA - A R, g2-subunit.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-151-img0007.jpg "Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat brain lysate showing immunolabeling of the ~45-47 kDa GABA - A R, g2-subunit.")
![Immunohistochemistry-Paraffin: GABA-A R gamma 2 Antibody [NB300-151] - Rat cerebellum.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Immunohistochemistry-Paraffin-NB300-151-img0005.jpg)
![Western Blot: GABA-A R gamma 2 Antibody [NB300-151] - 10 ug of rat hippocampal lysate showing immunolabeling of the ~46k gamma2-subunit of the GABA-A R.](http://images.novusbio.com/fullsize/GABA-A-R-gamma-2-Antibody-Western-Blot-NB300-151-img0006.jpg)
![Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP1-78292-img0005.jpg "Western Blot: Notch-1 Antibody [NBP1-78292] - Mouse total protein lysates (50 ug) were separated on 8% SDS-PAGE. Notch-1 (NBP1-78292SS) antibody was used at 1:500 dilution. Actin (1:10,000) was used as normalizer. WB image submitted by a verified customer review.")
![Immunohistochemistry: Notch-1 Antibody [NBP1-78292] - Staining of Notch1 in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Immunohistochemistry-NBP1-78292-img0003.jpg)
![Western Blot: Notch-1 Antibody [NBP1-78292] - Analysis of Cleaved Notch1 in Jurkat cell lysate.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Western-Blot-NBP1-78292-img0002.jpg)
![Simple Western: Notch-1 Antibody [NBP1-78292] - Lane view shows a specific band for Notch-1 in 0.5 mg/ml of Jurkat lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Notch-1-Antibody-Simple-Western-NBP1-78292-img0004.jpg)
![Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Western-Blot-H00026330-M01-img0009.jpg "Western Blot: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of GAPDHS expression in transfected 293T cell line by GAPDS monoclonal antibody (M01), clone 2E3-2E10.Lane 1: GAPDHS transfected lysate(44.5 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Analysis of monoclonal antibody to GAPDHS on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Immunohistochemistry-Paraffin-H00026330-M01-img0007.jpg)
![Sandwich ELISA: GAPDH-2 Antibody (2E3-2E10) [H00026330-M01] - Detection limit for recombinant GST tagged GAPDHS is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-2E3-2E10-Sandwich-ELISA-H00026330-M01-img0008.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding SYNJ1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding SYNJ1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol and centrosome. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87842-img0022.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0020.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cerebellum shows moderate positivity in neuropil as well as in a subset of neurons.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0017.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human cerebral cortex shows positivity in neuropil as well as moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0018.jpg)
![Immunohistochemistry-Paraffin: Synaptojanin 1 Antibody [NBP1-87842] - Staining of human rectum shows moderate cytoplasmic positivity in neurons in peripheral ganglia.](http://images.novusbio.com/fullsize/Synaptojanin-1-Antibody-Immunohistochemistry-Paraffin-NBP1-87842-img0019.jpg)
![Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Park7-DJ-1-Antibody-Western-Blot-NB100-1194-img0007.jpg "Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of HeLa (A) and Jurkat (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Park7/DJ-1 Antibody [NB100-1194] - (0.001ug/ml) staining of Human Cerebellum (A), Human Frontal Cortex (B), Human Hippocampus (C) Mouse Fetal Brain (D), Mouse Brain (E) and Rat Brain (F) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145588.jpg)
![Immunocytochemistry/Immunofluorescence: Park7/DJ-1 Antibody [NB100-1194] - Immunofluorescence staining of MCF7 (A) and Hela (B) cells with 5ug/ml NB100-1194 antibody. Detected with Rabbit anti-goat IgG-Alexafluor488 antibody at 1:1000. Nuclei Counterstained with DAPI.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-1311202311455816.jpg)
![Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - (7ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145587.jpg)
![Immunohistochemistry-Paraffin: Park7/DJ-1 Antibody [NB100-1194] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.](http://images.novusbio.com/fullsize/nb100-1194_goat-polyclonal-park7-dj-1-antibody-131120231145584.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0011.jpg "Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F9NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0008.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B in human prostate](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0010.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0007.jpg)
![Western Blot: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Total protein from human brain (lane 1) and mouse brain (lane 2) was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane for 30 minutes and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-S100B in 1% milk and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Western-Blot-NBP2-45225-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with S100B (15F4NB) at a 1:100 dilution overnight at 4 degrees Celsius and detected with Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunocytochemistry-Immunofluorescence-NBP2-45225-img0003.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis of S100B (15F4BN) in human brain tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0004.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - Analysis in human prostate tissue.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0005.jpg)
![Immunohistochemistry-Paraffin: S100B Antibody (15F4NB) - Azide Free [NBP2-45225] - IHC analysis of S100B (15F9NB) in human brain.](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Immunohistochemistry-Paraffin-NBP2-45225-img0009.jpg)
![Flow Cytometry: S100B Antibody (15F4NB) [Azide Free] [NBP2-45225] - Staining of A431 cells (1 x 10^6 cells/ml) with S100B antibody, clone 15F4.1D2 (orange) stained at a dilution of 1:500. Detected with a GtxRb Dylight 488 secondary. Shown with the secondary control (blue).](http://images.novusbio.com/fullsize/S100B-Antibody-15F4NB-Azide-Free-Flow-Cytometry-NBP2-45225-img0002.jpg)
![Western Blot: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of extracts from C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:200). Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-675-img0012.jpg "Western Blot: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of extracts from C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:200). Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - Analysis of acetone-fixed, frozen mouse ear skin section using anti-PGP9.5 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunofluorescence-NB300-675-img0014.jpg)
![Immunohistochemistry: PGP9.5 / UCHL-1 Antibody [NB300-675] - Human brain, Substantia Nigra, Pigmented Neuron, 40X.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunohistochemistry-NB300-675-img0010.jpg)
![Western Blot: PGP9.5 / UCHL-1 Antibody [NB300-675] - Detection of UCHL1 in mouse brain lysate.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Western-Blot-NB300-675-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - In Neuro2a cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-675-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: UCH-L1/PGP9.5 Antibody [NB300-675] - Confocal analysis of C6 cells using PGP9.5 / UCHL-1 antibody (NB300-675, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue). Image from verified customer review.](http://images.novusbio.com/fullsize/PGP9.5---UCHL-1-Antibody-Immunocytochemistry-Immunofluorescence-NB300-675-img0013.jpg)