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![Western Blot: TRPM7 Antibody [NBP1-46829] - Whole cell lysate (50 ug) from K-562, MCF-7, HeLa, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRPM7 antibody used for WB at 1 ug/ml. Detection: hemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Western-Blot-NBP1-46829-img0002.jpg "Western Blot: TRPM7 Antibody [NBP1-46829] - Whole cell lysate (50 ug) from K-562, MCF-7, HeLa, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRPM7 antibody used for WB at 1 ug/ml. Detection: hemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation: TRPM7 Antibody [NBP1-46829] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRPM7 antibody used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TRPM7 was also immunoprecipitated by rabbit anti-TRPM7 antibody BL9785, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 1 minute (B).](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunoprecipitation-NBP1-46829-img0001.jpg)
![Immunoprecipitation: TRPM7 Antibody [NBP1-46829] - Detection of human TRPM7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRPM7 antibody NBP1-46829 (lot NBP1-46829-3) used for IP at 6 ug per reaction. TRPM7 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46829-2). For blotting immunoprecipitated TRPM7, NBP1-46829 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/TRPM7-Antibody-Immunoprecipitation-NBP1-46829-img0003.jpg)
![Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] - Image of ATP5A Antibody (5G11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26491_rabbit-atp5a-mab-5g11-26220241422526.jpg "Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] - Image of ATP5A Antibody (5G11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")
![Immunohistochemistry: ATP5A Antibody (5G11) [NBP3-26491] - Image of ATP5A Antibody (5G11) diluted at 1:100 and staining in paraffin-embedded human liver tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26491_rabbit-atp5a-mab-5g11-262202415355739.jpg)
![Western Blot: ATP5A Antibody (5G11) [NBP3-26491] - Positive Western Blot detected in: HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, HL60 whole cell lysate, Mouse Heart tissue, Mouse Brain tissue. All lanes: ATP5A Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 60, 55, 58 kDa Observed band size: 60 kDa](http://images.novusbio.com/fullsize/nbp3-26491_rabbit-atp5a-mab-5g11-28220241144262.jpg)
![Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP1-44264] - Lane 1: Brain Extract. Load: 10 ug per lane. Primary antibody: KMO antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 58 kDa for KMO. Other band(s): Other bands at lower molecular weights. These bands are all specifically blocked by KMO peptide.](http://images.novusbio.com/fullsize/Kynurenine-3-Monooxygenase-KMO-Antibody-Western-Blot-NBP1-44264-img0003.jpg "Western Blot: Kynurenine 3-Monooxygenase/KMO Antibody [NBP1-44264] - Lane 1: Brain Extract. Load: 10 ug per lane. Primary antibody: KMO antibody at 1ug/mL for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 58 kDa for KMO. Other band(s): Other bands at lower molecular weights. These bands are all specifically blocked by KMO peptide.")
![Western Blot: CHMP2B Antibody [NBP2-15886] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:2000.](http://images.novusbio.com/fullsize/CHMP2B-Antibody-Western-Blot-NBP2-15886-img0001.jpg "Western Blot: CHMP2B Antibody [NBP2-15886] - Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE gel, diluted at 1:2000.")
![Immunocytochemistry/Immunofluorescence: CHMP2B Antibody [NBP2-15886] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using CHMP2B antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/CHMP2B-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-15886-img0002.jpg)
![Western Blot: CHMP2B Antibody [NBP2-15886] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with CHMP2B antibody (NBP2-15886) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-15886_rabbit-polyclonal-chmp2b-antibody-91020242091428.jpg)
![Western Blot: ANKS1B Antibody - BSA Free [NBP3-48144] - A549 cells were subjected to SDS PAGE followed by western blot with (ANKS1B Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-48144_rabbit-anks1b-pab-bsa-free-28420251138017.jpg "Western Blot: ANKS1B Antibody - BSA Free [NBP3-48144] - A549 cells were subjected to SDS PAGE followed by western blot with (ANKS1B Antibody) at dilution of 1:1000")
![Immunohistochemistry: ANKS1B Antibody - BSA Free [NBP3-48144] - Immunohistochemistry of paraffin-embedded human testis tissue slide using (ANKS1B Antibody) at dilution of 1:50](http://images.novusbio.com/fullsize/nbp3-48144_rabbit-anks1b-pab-bsa-free-28420251138084.jpg)
![Western Blot: MAO-A Antibody [H00004128-B02P] - Analysis of MAOA expression in human liver.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Western-Blot-H00004128-B02P-img0001.jpg "Western Blot: MAO-A Antibody [H00004128-B02P] - Analysis of MAOA expression in human liver.")
![Western Blot: MAO-A Antibody [H00004128-B02P] - Analysis of MAOA expression in transfected 293T cell line by MAOA polyclonal antibody. Lane 1: MAOA transfected lysate(57.97 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/MAO-A-Antibody-Western-Blot-H00004128-B02P-img0002.jpg)
![Western Blot: Brevican Antibody [H00063827-B01P] - Analysis of BCAN expression in transfected 293T cell line by BCAN polyclonal antibody. Lane 1: BCAN transfected lysate(100.21 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Brevican-Antibody-Western-Blot-H00063827-B01P-img0002.jpg "Western Blot: Brevican Antibody [H00063827-B01P] - Analysis of BCAN expression in transfected 293T cell line by BCAN polyclonal antibody. Lane 1: BCAN transfected lysate(100.21 KDa). Lane 2: Non-transfected lysate.")
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody [HL3638] (NBP3-48830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48830_rabbit-uqcrc2-mab-hl3638-western-blot-1411202518395416.jpg "Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody [HL3638] (NBP3-48830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody [HL3638] (NBP3-48830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48830_rabbit-uqcrc2-mab-hl3638-western-blot-1411202518281120.jpg)
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody [HL3638] (NBP3-48830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48830_rabbit-uqcrc2-mab-hl3638-western-blot-1411202518372710.jpg)
![Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody [HL3749] (NBP3-48841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48841_rabbit-uqcrc2-mab-hl3749-western-blot-141120251824330.jpg "Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody [HL3749] (NBP3-48841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody [HL3749] (NBP3-48841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48841_rabbit-uqcrc2-mab-hl3749-western-blot-1411202518443410.jpg)
![Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC2 antibody [HL3749] (NBP3-48841) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-48841_rabbit-uqcrc2-mab-hl3749-western-blot-1411202518371522.jpg)
![Immunocytochemistry/Immunofluorescence: LRPPRC Antibody (HL2056) - Azide and BSA Free [NBP3-25551] - LRP130 antibody [HL2056] detects LRP130 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP130 stained by LRP130 antibody [HL2056] (NBP3-25551) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25551_rabbit-lrpprc-mab-hl2056-azide-and-bsa-free-212024939124.jpg "Immunocytochemistry/Immunofluorescence: LRPPRC Antibody (HL2056) - Azide and BSA Free [NBP3-25551] - LRP130 antibody [HL2056] detects LRP130 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: LRP130 stained by LRP130 antibody [HL2056] (NBP3-25551) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .")
![<b>Orthogonal Strategies Validation. </b>Western Blot: LRPPRC Antibody (HL2056) - Azide and BSA Free [NBP3-25551] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [HL2056] (NBP3-25551) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25551_rabbit-lrpprc-mab-hl2056-azide-and-bsa-free-2120249374964.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: LRPPRC Antibody (HL2056) - Azide and BSA Free [NBP3-25551] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with LRP130 antibody [HL2056] (NBP3-25551) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25551_rabbit-lrpprc-mab-hl2056-azide-and-bsa-free-212024939159.jpg)
