| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11438 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81114-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52678-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75907SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12063-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12025-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (YMF323.6) [NBP2-81114] - Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with [NBP2-81114]. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YMF323.6 [NBP2-81114] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81114, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-YMF323-6-Immunocytochemistry-Immunofluorescence-NBP2-81114-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (YMF323.6) [NBP2-81114] - Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with [NBP2-81114]. Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of YMF323.6 [NBP2-81114] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81114, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (hu5c8 (Ruplizumab)) - Chimeric [NBP2-52678] - Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Ruplizimab (NBP2-52678, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-hu5c8-Ruplizumab-Chimeric-Flow-Cytometry-NBP2-52678-img0003.jpg "Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (hu5c8 (Ruplizumab)) - Chimeric [NBP2-52678] - Flow-cytometry using the anti-CD40L research biosimilar antibody Ruplizumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Ruplizimab (NBP2-52678, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Western Blot: CD40 Ligand/TNFSF5 Antibody (hu5c8 (Ruplizumab)) [NBP2-75907] - Rat spleen (lane 1), rat muscle (lane 2), rat bladder (lane 3), human breast tumour (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75907 at 1 ug/ml before being detected by a secondary antibody. The expected band size (29 kDa) is seen in the human-derived samples, as well as bands corresponding to dimers and other multimers.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-hu5c8-Ruplizumab-Western-Blot-NBP2-75907-img0001.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody (hu5c8 (Ruplizumab)) [NBP2-75907] - Rat spleen (lane 1), rat muscle (lane 2), rat bladder (lane 3), human breast tumour (lane 4) and human thyroid tumour (lane 5) samples were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75907 at 1 ug/ml before being detected by a secondary antibody. The expected band size (29 kDa) is seen in the human-derived samples, as well as bands corresponding to dimers and other multimers.")
![Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Using the Anti-CD45RA antibody OTH-74D4 (Ab01502). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with CD45RA antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OTH-74D4 (Ab01502-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD45RA-Antibody-OTH-74D4-Chimeric-Flow-Cytometry-NBP3-12063-img0001.jpg "Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Using the Anti-CD45RA antibody OTH-74D4 (Ab01502). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with CD45RA antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OTH-74D4 (Ab01502-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunofluorescence: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Staining of Jukat cells with anti-CD45RA (Ab01502) OTH-74D4 Immunofluorescence analysis of paraformaldehyde fixed Jukat cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OTH-74D4 (Ab01502-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01502-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/CD45RA-Antibody-OTH-74D4-Chimeric-Immunofluorescence-NBP3-12063-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric [NBP3-12025] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of chi220 (NBP3-12025) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12025, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-chi220-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12025-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric [NBP3-12025] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of chi220 (NBP3-12025) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12025, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (chi220) - Chimeric - Azide and BSA Free [NBP3-12025] - Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12025 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12025_rabbit-monoclonal-cd40-ligand-tnfsf5-antibody-chi220-chimeric-74202522113024.png)
![SDS-Page: Recombinant Human CD11b Protein [H00003684-Q01] - 12.5% SDS-PAGE using Recombinant Human CD11b Protein [H00003684-Q01] stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-CD11b-Protein-SDS-Page-H00003684-Q01-img0002.jpg "SDS-Page: Recombinant Human CD11b Protein [H00003684-Q01] - 12.5% SDS-PAGE using Recombinant Human CD11b Protein [H00003684-Q01] stained with Coomassie Blue.")
![Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-29202410334038.jpg "Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CD45 Antibody (PSH03-64) [NBP3-32448] - Flow cytometric analysis of human PBMC labeling human CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32448, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-392024884642.jpg)
