| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54743-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11592 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02957-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-04117-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82784-25ul | |||
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0008.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human liver shows no positivity in hepatocytes as expected.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Analysis in human stomach and liver tissues. Corresponding Carbonic Anhydrase IX/CA9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0004.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0005.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0006.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human gallbladder shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0007.jpg)
![Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] - Untreated (-) and treated (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Smad2-[p-Ser465--p-Ser467]-Antibody-Western-Blot-NBP3-13273-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] - Untreated (-) and treated (+) MCF-7 whole cell extract (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Smad2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Smad2 [p Ser465, p Ser467] Antibody [NBP3-13273] - Smad2 (phospho Ser465/Ser467) antibody detects Smad2 (phospho Ser465/Ser467) protein at nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Smad2 (phospho Ser465/Ser467) stained by Smad2 (phospho Ser465/Ser467) antibody (NBP3-13273) diluted at 1:1500. Red: phalloidin, a cytoskeleton marker, diluted at 1:100. Scale bar= 10 um.](http://images.novusbio.com/fullsize/Smad2-[p-Ser465--p-Ser467]-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13273-img0002.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (PN-15) [NBP2-45238] - Human Renal Cell Carcinoma stained with PNA Monoclonal Antibody (PN-15).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-PN-15-Immunohistochemistry-Paraffin-NBP2-45238-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (PN-15) [NBP2-45238] - Human Renal Cell Carcinoma stained with PNA Monoclonal Antibody (PN-15).")
![Western Blot: Smad2 Antibody [NBP3-02957] - Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (NBP3-02957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.](http://images.novusbio.com/fullsize/Smad2-Antibody-Western-Blot-NBP3-02957-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Smad2 Antibody [NBP3-02957] - Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (NBP3-02957) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.")
![Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.](http://images.novusbio.com/fullsize/HRAS-Antibody-Western-Blot-NBP3-04117-img0003.jpg "Western Blot: HRAS Antibody [NBP3-04117] - Analysis of extracts of various cell lines, using HRAS antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit.")
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric cancer using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0002.jpg)
![Immunohistochemistry-Paraffin: HRAS Antibody [NBP3-04117] - Paraffin-embedded human gastric using HRAS antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/HRAS-Antibody-Immunohistochemistry-Paraffin-NBP3-04117-img0001.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/CD63-Antibody-Immunohistochemistry-Paraffin-NBP1-82784-img0004.jpg "Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human lung shows moderate to strong cytoplasmic positivity in macrophages.")
![Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human bone marrow shows moderate to strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/CD63-Antibody-Immunohistochemistry-Paraffin-NBP1-82784-img0001.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human spleen shows moderate to strong cytoplasmic positivity in cells in red pulp.](http://images.novusbio.com/fullsize/CD63-Antibody-Immunohistochemistry-Paraffin-NBP1-82784-img0002.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody [NBP1-82784] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD63-Antibody-Immunohistochemistry-Paraffin-NBP1-82784-img0003.jpg)
