| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-16891-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50300-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47937-0.1mg | |||
| IHC-Prediluted | NBP2-48022 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76563-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54743-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11592 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11591 | |||
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0004.jpg "Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN (phospho Thr366/Ser370) antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/PTEN-[p-Thr366--p-Ser370]-Antibody-Western-Blot-NBP3-12919-img0003.jpg)
![Western Blot: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PTEN antibody (NBP3-12919) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-12919_rabbit-pten-p-thr-366-p-ser-370-pab-9102024204126.jpg)
![Immunocytochemistry/ Immunofluorescence: PTEN [p Thr366, p Ser370] Antibody [NBP3-12919] - PTEN antibody detects PTEN protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: PTEN stained by PTEN antibody (NBP3-12919) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-12919_rabbit-pten-p-thr-366-p-ser-370-pab-91020242001095.jpg)
![Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Smad2-[p-Ser467]-Antibody-Western-Blot-NBP3-16891-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Smad2 [p Ser467] Antibody [NBP3-16891] - Western blot analysis of extracts of HeLa cells, using Smad2 antibody (NBP3-16891) at 1:1000 dilution.Hela cells were treated by TGF-beta (10 ng/ml) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Rat testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125711..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Mouse testis tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125830..jpg)
![Immunohistochemistry-Paraffin:Smad2 [p Ser467] Antibody [NBP3-16891] - Human kidney tissue using Phospho-Smad2-S467 Rabbit pAb at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/antibody/nbp3-16891_rabbit-smad2-p-ser-467-pab-immunohistochemistry-paraffin-1392024125949..jpg)
![Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-288202412461629.jpg "Western Blot: PTEN Antibody (JE51-96) [NBP3-32873] - Western blot analysis of PTEN on different lysates with Rabbit anti-PTEN antibody (NBP3-32873) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: MCF7 cell lysate Lane 3: MDA-MB-468 cell lysate (negative) Lane 4: A431 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C6 cell lysate Lane 7: PC-12 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 47 kDa Observed band size: 55 kDa Exposure time: 2 minutes 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32873) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PTEN Antibody (JE51-96) [NBP3-32873] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-PTEN antibody (NBP3-32873) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32873) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29820241492644.jpg)
![Flow Cytometry: PTEN Antibody (JE51-96) [NBP3-32873] - Flow cytometric analysis of A431 cells labeling PTEN. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32873, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32873_rabbit-pten-mab-je51-96-29202416372137.jpg)
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA.<br/><br/> Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA.<br/><br/> Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution.<br/><br/> Lane 1: THP-1 cell lysate<br/> Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate<br/><br/> Lysates/proteins at 10 ug/Lane.<br/><br/> Predicted band size: 31 kDa<br/> Observed band size: 31 kDa<br/><br/> Exposure time: 1 minute;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (NKI/C3 + LAMP3/968) [NBP2-44811] - Formalin-fixed, paraffin-embedded human Melanoma metastasized to Lung stained with](http://images.novusbio.com/fullsize/CD63-Antibody-NKI-C3-+-LAMP3-968-Immunohistochemistry-Paraffin-NBP2-44811-img0004.jpg "Immunohistochemistry-Paraffin: CD63 Antibody (NKI/C3 + LAMP3/968) [NBP2-44811] - Formalin-fixed, paraffin-embedded human Melanoma metastasized to Lung stained with")
![Immunohistochemistry-Paraffin: CD63 Antibody (NKI/C3 + LAMP3/968) [NBP2-44811] - Formalin-fixed, paraffin-embedded human Testicular Ca stained with CD63 Antibody (NKI/C3 + LAMP3/968)](http://images.novusbio.com/fullsize/CD63-Antibody-NKI-C3-+-LAMP3-968-Immunohistochemistry-Paraffin-NBP2-44811-img0005.jpg)
![Immunohistochemistry-Paraffin: CD63 Antibody (NKI/C3 + LAMP3/968) [NBP2-44811] - Human Prostate Carcinoma stained with CD63 Monoclonal Antibody (NKI/C3 + LAMP3/968)](http://images.novusbio.com/fullsize/CD63-Antibody-NKI-C3-+-LAMP3-968-Immunohistochemistry-Paraffin-NBP2-44811-img0002.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody [NBP2-76563] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/VEGF-Antibody-Immunohistochemistry-Paraffin-NBP2-76563-img0006.jpg "Immunohistochemistry-Paraffin: VEGF Antibody [NBP2-76563] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: VEGF Antibody [NBP2-76563] - Staining of human adipose tissue shows moderate cytoplasmic positivity in adipocytes.](http://images.novusbio.com/fullsize/VEGF-Antibody-Immunohistochemistry-Paraffin-NBP2-76563-img0002.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody [NBP2-76563] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/VEGF-Antibody-Immunohistochemistry-Paraffin-NBP2-76563-img0003.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody [NBP2-76563] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/VEGF-Antibody-Immunohistochemistry-Paraffin-NBP2-76563-img0004.jpg)
![Immunohistochemistry-Paraffin: VEGF Antibody [NBP2-76563] - Staining of human prostate shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/VEGF-Antibody-Immunohistochemistry-Paraffin-NBP2-76563-img0005.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0008.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human liver shows no positivity in hepatocytes as expected.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Analysis in human stomach and liver tissues. Corresponding Carbonic Anhydrase IX/CA9 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0004.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human colon shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0005.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human duodenum shows moderate membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0006.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody [NBP2-54743] - Staining of human gallbladder shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-Immunohistochemistry-Paraffin-NBP2-54743-img0007.jpg)
![ELISA: Mouse Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) [NBP2-71283] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase IX/CA9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-71283_mouse-carbonic-anhydrase-ix-ca9-elisa-kit-colorimetric-132202416232325.jpg "ELISA: Mouse Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) [NBP2-71283] - Samples were spiked with high concentrations of Mouse Carbonic Anhydrase IX/CA9 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Carbonic Anhydrase IX/CA9 ELISA Kit (Colorimetric) [NBP2-71283] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-71283_mouse-carbonic-anhydrase-ix-ca9-elisa-kit-colorimetric-432024973616.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (PN-15) [NBP2-45238] - Human Renal Cell Carcinoma stained with PNA Monoclonal Antibody (PN-15).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-PN-15-Immunohistochemistry-Paraffin-NBP2-45238-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (PN-15) [NBP2-45238] - Human Renal Cell Carcinoma stained with PNA Monoclonal Antibody (PN-15).")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-CA9-781-Immunohistochemistry-Paraffin-NBP2-45237-img0001.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Human Renal Cell Carcinoma stained with CAIX Monoclonal Antibody (CA9/781).")
![Western Blot: Carbonic Anhydrase IX/CA9 Antibody (CA9/781) [NBP2-45237] - Analysis of HCT116 Cell Lysate using CAIX Monoclonal Antibody (CA9/781). As indicated by the arrow, a specific band was detected at a molecular weight of approximately 50 kDa.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-IX-CA9-Antibody-CA9-781-Western-Blot-NBP2-45237-img0002.jpg)