| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71488 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70954 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61832-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-25149SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-2519SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84797-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-90291-25ul | |||
![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody [NBP1-33714] - SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP25 protein stained by SNAP25 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33714-img0012.jpg "Immunocytochemistry/Immunofluorescence: SNAP25 Antibody [NBP1-33714] - SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP25 protein stained by SNAP25 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Frozen: SNAP25 Antibody [NBP1-33714] - Frozen sectioned E13.5 Rat brain. Green: SNAP25 protein stained by SNAP25 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Frozen-NBP1-33714-img0011.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0003.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Mouse fore brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Rat hind brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0010.jpg)
![Immunohistochemistry-Whole-Mount: SNAP25 Antibody [NBP1-33714] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: SNAP25 stained by SNAP25 antibody diluted at 1:50.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70 C](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunohistochemistry-whole-mount-127202384126.jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-12720238431..jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-127202384456..jpg)
![Western Blot: ERp72 Antibody (2B9) [NBP2-01652] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERp72 monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Western-Blot-NBP2-01652-img0009.jpg "Western Blot: ERp72 Antibody (2B9) [NBP2-01652] Analysis of extracts (35ug) from 9 different cell lines by using anti-ERp72 monoclonal antibody.")
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human tonsil using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0008.jpg)
![Western Blot: ERp72 Antibody (2B9) [NBP2-01652] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERp72 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ERp72.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Western-Blot-NBP2-01652-img0001.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0002.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0003.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0004.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human liver tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0005.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human lymphoma tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0006.jpg)
![Immunohistochemistry-Paraffin: ERp72 Antibody (2B9) [NBP2-01652] - Staining of paraffin-embedded Human pancreas tissue using anti-ERp72 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ERp72-Antibody-2B9-Immunohistochemistry-Paraffin-NBP2-01652-img0007.jpg)
![Western Blot: ERp72 Antibody (2B9) [NBP2-01652] - Western Blot of lysate of PC-3 cells. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-01652_mouse-erp72-mab-oti2b9-western-blot-672023153749..jpg)
![Immunocytochemistry/Immunofluorescence: Mouse ERp72 mAb (OTI2B9) [NBP2-01652] - Primary mouse hepatocytes stained with ERp72 mAb. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-01652_mouse-erp72-mab-oti2b9-immunocytochemistry-immunofluorescence-7122024113558..png)
![Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Western-Blot-NBP2-02190-img0011.jpg "Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)")
![Immunocytochemistry/Immunofluorescence: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunocytochemistry-Immunofluorescence-NBP2-02190-img0008.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0007.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Flow-Cytometry-NBP2-02190-img0010.jpg)
![Western Blot: Hsp70 Antibody (3G9) [NBP2-02190] Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Western-Blot-NBP2-02190-img0009.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0002.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0003.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0004.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0005.jpg)
![Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Hsp70-Antibody-3G9-Immunohistochemistry-Paraffin-NBP2-02190-img0006.jpg)
![Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Western-Blot-NB300-555-img0005.jpg "Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. TRAP1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0006.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (TRAP1-6) [NB300-555] - Normal biopsies of deparaffinized Human liver tissue, dilution of 1:20.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunohistochemistry-Paraffin-NB300-555-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Immunolocalization of TRAP1 in PC-3-M cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0003.jpg)
![Immunohistochemistry: TRAP1 Antibody (TRAP1-6) [NB300-555] - Representative images of IHF staining of EP2 or EP4 (green) and TRAP1 (NB300-555) (red) in subchondral bone 2 weeks post-sham or ACLT surgery (left) and quantitative analysis (right). The white arrows indicate TRAP-positive osteoclasts expressing EP2 or EP4. Scale bars, 20 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-25520231539518.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - PGE2 regulates migration & osteoclast differentiation through the G alpha s/PI3K/AKT/MAPK signaling pathways downstream of EP4. PGE2 regulates migration & osteoclast differentiation independent of beta -arrestin1 (a) & beta -arrestin2 (b) but via G alpha s (c). BMMs from the WT & littermate beta -arrestin1- & beta -arrestin2-knockout mice were used to generate osteoclasts by stimulation with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL & incubation with 100 nmol·L−1 PGE2. For G alpha s experiments, osteoclasts were generated from the GMMs of the G alpha sfl/fl controls & G alpha sfl/fl; LysM-cre (G alpha sLysM) mice. A differentiation assay (TRAP staining) (left) & the corresponding quantitative analysis (right). Scale bars, 50 μm. Error bars are the mean ± s.d. n = 3. Two-way ANOVA followed by Tukey’s t tests. Scale bars, 50 μm. d cAMP production was measured via ELISAs for osteoclasts derived from the Ep4fl/fl & Ep4LysM mice. BMMs from the two mouse strains were isolated & stimulated with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL to differentiate into osteoclasts & incubated with 100 nmol·L−1 PGE2 for 30 min prior to cAMP measurements. Error bars are the mean ± s.d. ***P < 0.001 by unpaired two-tailed Student’s t test. The experiment was performed with three biological replicates. e Representative images of the indicated protein expression by western blotting for osteoclasts generated using BMMs of Ep4fl/fl & Ep4LysM mice. The cells were treated either with osteoclastogenic media (10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL) alone, with PGE2 (100 nmol·L−1), or PGE2 with IBMX (1 mmol·L−1) for 3 h. The experiments were performed with three biological replicates Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-310202416212330.jpg)
![Western Blot: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Western-Blot-NBP2-70954-img0002.jpg "Western Blot: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)")
![Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-70954-img0004.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded pancreas tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0009.jpg)
![Flow Cytometry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Analysis of permeabilized Jurkat cells, using anti-HSPA1A antibody( Red), compared to an isotype control (green), and a PBS control (blue).(1:100)](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70954-img0003.jpg)
![Western Blot: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Hsp70 monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Western-Blot-NBP2-70954-img0001.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0005.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded endometrium tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0006.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded Kidney tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0007.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded liver tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0008.jpg)
![Immunohistochemistry: HSP70/HSPA1A Antibody (OTI3G9) - Azide and BSA Free [NBP2-70954] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/HSP70-HSPA1A-Antibody-OTI3G9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70954-img0010.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0009.jpg "Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0005.jpg)
![Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunohistochemistry-NBP2-61832-img0007.jpg)
![Flow Cytometry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Flow-Cytometry-NBP2-61832-img0002.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0008.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), C6 (4), Jurkat (5), Hela (6), COS7 (7), and Jurkat (8) cell lysate.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Western-Blot-NBP2-61832-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of GC-7901 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunocytochemistry-Immunofluorescence-NBP2-61832-img0004.jpg)
![Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded cervical cancer tissues using RAN mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-Immunohistochemistry-NBP2-61832-img0006.jpg)
![ELISA: Ran Antibody (8D1A6) [NBP2-61832] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/Ran-Antibody-8D1A6-ELISA-NBP2-61832-img0001.jpg)
![Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - RanGTPase detected with Mouse Monoclonal Ran Antibody (8D1A6) in HEK293T and LNCaP C42. Lanes are labeled as follows: 1. Recombinant protein 2. HEK239T 3. HEK293T constitutively expressing GFP-tagged construct 4. LNCaP C42 5. LNCaP C42. Recombinant RAN protein is loaded with HEK293T and LNCaP C42 prostate cell line. Cell lysates run on a 4-15% mini gel. The ladder is Precision Plus Protein Standards Kaleidoscope. Proteins were detected with a secondary anti-mouse conjugated with Alexa Fluor 680 and detected with infrared scanner. Image from verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-61832_mouse-monoclonal-ran-antibody-8d1a6-western-blot-162023155342.jpg)
![Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Knockout-Validated-NBP2-25149-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP27 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:5,000 of Mouse Anti-Human HSP27 Monoclonal Antibody (Catalog # NBP2-25149) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (catalog number HAF018). Specific band was detected for HSP27 at approximately 27 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of tissue or whole cell lysates using mouse mAb to HSP27, NBP2-25149, dilution 1:10,000, in green. [1] protein standard (red), [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HEK293, [6] HeLa, [7] SH-SY5Y cells. The strong single band at ~27 kDa corresponds to the HSP27 protein, detected only in human cell lines, since this antibody is human specific and unreactive with rodent HSP27. The blot was simultaneously probed with rabbit pAb to HSP-60, dilution 1:5,000 in red. A strong 60 kDa band is present in all preparations.](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Western-Blot-NBP2-25149-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: HSP27 Antibody (6H11) [NBP2-25149] - Analysis of HeLa cells stained with mouse HSP27 mAb, dilution 1:2000 (Green), and costained with chicken HSP60 pAb, dilution 1:2000 (Red). DAPI staining of nuclear DNA (Blue). The HSP27 antibody produces strong cytoplasm staining, while the HSP60 antibody specifically labels mitochondria](http://images.novusbio.com/fullsize/HSP27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0008.jpg)
![Flow Cytometry: Hsp27 Antibody (6H11) [NBP2-25149] - Flow cytometric staining of 1 x 10^6 MCF-7 cells using Hsp27 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Flow-Cytometry-NBP2-25149-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Hsp27 Antibody (6H11) [NBP2-25149] - HeLa cells stained with NBP2-25149 (red), and counterstained with chicken polyclonal antibody to Vimentin NB300-223 (green) and DNA (blue). NBP2-25149 reveals strong cytoplasmic staining and penetrates into the Actin rich ruffled margins, while the Vimentin antibody reveals cytoplasmic intermediate filaments.](http://images.novusbio.com/fullsize/Hsp27-Antibody-6H11-Immunocytochemistry-Immunofluorescence-NBP2-25149-img0003.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0007.jpg "<b>Biological Strategies Validation. </b>Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Ethanol induces the expression of heat shock proteins (HSPs) in primary astrocyte culture. Increase in alphabeta-crystallin protein levels after treatment for 2 h with 60 mmol/L ethanol (EtOH) or 42C heat shock (HS) in primary astrocyte culture. alpha-tubulin used as an internal standard. The bar graph to the right of the immunoblots represents the quantification of immunoreactive bands intensities normalized to the internal standard, expressed in arbitrary optical density (OD) units. The data are the mean +/- SEM of normalized relative OD values analyzed by one-way ANOVA with Dunnett's multiple comparison post hoc test, n = 3 (significantly differently at the level of *P < 0.05, **P < 0.01, ***P < 0.001). Image collected and cropped by CiteAb from the following publication (//doi.wiley.com/10.1002/brb3.125) licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - CRYAB antibody was tested in SHSH5Y cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Immunocytochemistry-Immunofluorescence-NB100-2519-img0004.jpg)
![Western Blot: AlphaB Crystallin/CRYAB Antibody [NB100-2519] - Detection of CryAB using NB 100-2519 (0.5ug/ml). Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.](http://images.novusbio.com/fullsize/AlphaB-Crystallin-CRYAB-Antibody-Western-Blot-NB100-2519-img0005.jpg)
![Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Western-Blot-NB100-41377-img0006.jpg "Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41377-img0005.jpg)
![Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/HSPA8-HSC71-Hsc70-Antibody-Immunohistochemistry-Paraffin-NB100-41377-img0008.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Analysis in human colon and liver tissues. Corresponding Dynamin 2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Dynamin 2 Antibody [NBP2-47477] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Western-Blot-NBP2-47477-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Dynamin 2 Antibody [NBP2-47477] - Staining of human cell line HeLa shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47477-img0004.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0012.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human colon shows high expression.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0006.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0010.jpg)
![Immunohistochemistry-Paraffin: Dynamin 2 Antibody [NBP2-47477] - Staining of human skin shows weak to moderate cytoplasmic positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Dynamin-2-Antibody-Immunohistochemistry-Paraffin-NBP2-47477-img0011.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-84797 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0023.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis in human thyroid gland and skeletal muscle tissues using NBP1-84797 antibody. Corresponding PDIA3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human gastrointestinal, prostate, skeletal muscle and thyroid gland using Anti-PDIA3 antibody NBP1-84797 (A) shows similar protein distribution across tissues to independent antibody NBP1-84796 (B).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0017.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: ERp57/PDIA3 Antibody [NBP1-84797] - Analysis using Anti-PDIA3 antibody NBP1-84797 (A) shows similar pattern to independent antibody NBP1-84796 (B).](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Western-Blot-NBP1-84797-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: ERp57/PDIA3 Antibody [NBP1-84797] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84797-img0007.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human prostate shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0018.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0019.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human small intestine shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0020.jpg)
![Immunohistochemistry-Paraffin: ERp57/PDIA3 Antibody [NBP1-84797] - Staining of human thyroid gland shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ERp57-PDIA3-Antibody-Immunohistochemistry-Paraffin-NBP1-84797-img0021.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding MDR1/ABCB1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0009.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Analysis in human adrenal gland and skeletal muscle tissues. Corresponding MDR1/ABCB1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human cell line A-431 shows localization to nucleoplasm & focal adhesion sites. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-90291-img0004.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0014.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0010.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human kidney shows moderate positivity in apical membrane in cells in distal tubules.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0011.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human liver shows weak membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0012.jpg)
![Immunohistochemistry-Paraffin: MDR1/ABCB1 Antibody [NBP1-90291] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/MDR1-ABCB1-Antibody-Immunohistochemistry-Paraffin-NBP1-90291-img0013.jpg)