| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1952-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77563SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44754 | |||
| Azide and BSA Free | NBP2-34670-0.1mg | |||
| Azide and BSA Free | NBP2-34670-0.2mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44433 | |||
| BSA Free | NBP2-42379-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77682SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47843-0.1mg | |||
| IHC-Prediluted | NBP2-48187 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76798SS | |||
| Azide and BSA Free | NBP2-80879 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08931 | |||
![Western Blot: LAMP-1/CD107a Antibody (LY1C6) [NB100-1952] - Western Blot analysis of Rat liver microsome lysate showing detection of LAMP-1/CD107a protein using Mouse Anti-LAMP-1/CD107a Monoclonal Antibody, Clone Ly1C6 (NB100-1952). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-LAMP-1/CD107a Monoclonal Antibody (NB100-1952) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-LY1C6-Western-Blot-NB100-1952-img0010.jpg "Western Blot: LAMP-1/CD107a Antibody (LY1C6) [NB100-1952] - Western Blot analysis of Rat liver microsome lysate showing detection of LAMP-1/CD107a protein using Mouse Anti-LAMP-1/CD107a Monoclonal Antibody, Clone Ly1C6 (NB100-1952). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-LAMP-1/CD107a Monoclonal Antibody (NB100-1952) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: LAMP-1/CD107a Antibody (LY1C6) [NB100-1952] - analysis using Mouse Anti-LAMP1 Monoclonal Antibody, Clone Ly1C6 . Tissue: transfected HeLa cells. Species: Human. Primary Antibody: Mouse Anti-LAMP1 Monoclonal Antibody at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Courtesy of: Robert H Edwards, U. of Cali, San Fran School of Medicine.](http://images.novusbio.com/fullsize/LAMP-1-CD107a-Antibody-LY1C6-Immunocytochemistry-Immunofluorescence-NB100-1952-img0008.jpg)
![Western Blot: HSP90 beta Antibody [NBP1-77563] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP1-77563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Knockdown-Validated-NBP1-77563-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HSP90 beta Antibody [NBP1-77563] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HSP90 beta knockout (KO) HeLa cell line. PVDF membrane was probed with 1 ug/ml of Rabbit Anti-Human HSP90 beta Polyclonal Antibody (Catalog # NBP1-77563) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for HSP90 beta at approximately 95 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: HSP90 beta Antibody [NBP1-77563] - Analysis of HSP901b in: 1. HeLa, 2. Ntera 2, 3. A431, 4. HepG2, 5. MCF7, 6. NIH/3T3, 7. PC12 and 8. COS7.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Western-Blot-NBP1-77563-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody [NBP1-77563] - HSP90-1B antibody was tested in HepG2 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77563-img0006.jpg)
![Immunohistochemistry: HSP90 beta Antibody [NBP1-77563] - Staining of HSP901b in mouse prostate using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Immunohistochemistry-NBP1-77563-img0004.jpg)
![Simple Western: HSP90 beta Antibody [NBP1-77563] - Simple Western lane view shows a specific band for Hsp90B in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-Simple-Western-NBP1-77563-img0007.jpg)
![Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Western-Blot-NBP2-32973-img0010.jpg "Western Blot: HSP60 Antibody (LK1) [NBP2-32973] - Western Blot Analysis of HeLa cell lysate using HSP60 Antibody (LK1)")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK1) [NBP2-32973] - Immunofluorescent staining of PFA-fixed MCF-7 cells. followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunocytochemistry-Immunofluorescence-NBP2-32973-img0009.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with HSP60 MAb (LK1)](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunohistochemistry-Paraffin-NBP2-32973-img0001.jpg)
![Flow Cytometry: HSP60 Antibody (LK1) [NBP2-32973] - Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells using HSP60 antibody (LK1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Flow-Cytometry-NBP2-32973-img0011.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (LK1) [NBP2-32973] - Formalin-fixed paraffin-embedded human pancreas stained with HSP60 Monoclonal Antibody (LK1).](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Immunohistochemistry-Paraffin-NBP2-32973-img0005.jpg)
![Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Simple-Western-NBP2-32973-img0002.jpg)
![Simple Western: HSP60 Antibody (LK1) [NBP2-32973] - Electropherogram image of the corresponding Simple Western lane. HSP60 antibody was used at 10 ug/ml dilution of HeLa and HepG2 lysates(s) respectively.](http://images.novusbio.com/fullsize/HSP60-Antibody-LK1-Simple-Western-NBP2-32973-img0004.jpg)
![Immunohistochemistry:PMP22 Antibody (OTI5D1)[NBP3-44912]](http://images.novusbio.com/fullsize/nbp3-44912_mouse-pmp22-mab-oti5d1-immunohistochemistry-1812026169532.jpg "Immunohistochemistry:PMP22 Antibody (OTI5D1)[NBP3-44912]")
![Immunohistochemistry:PMP22 Antibody (OTI5D1)[NBP3-44912]](http://images.novusbio.com/fullsize/nbp3-44912_mouse-pmp22-mab-oti5d1-immunohistochemistry-18120261618483.jpg)
![Immunohistochemistry:PMP22 Antibody (OTI5D1)[NBP3-44912]](http://images.novusbio.com/fullsize/nbp3-44912_mouse-pmp22-mab-oti5d1-immunohistochemistry-18120261615210.jpg)
![Western Blot:PMP22 Antibody (OTI5D1)[NBP3-44912]](http://images.novusbio.com/fullsize/nbp3-44912_mouse-pmp22-mab-oti5d1-western-blot-181202616192816.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in human prostate and skeletal muscle tissues using NBP2-42379 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0021.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in human prostate and skeletal muscle tissues using NBP2-42379 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Western-Blot-NBP2-42379-img0016.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in U251 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0010.jpg)
![Western Blot: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Western-Blot-NBP2-42379-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in HeLa cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human cell line A431 showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in MCF7 cell line showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining in U2OS cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunocytochemistry-Immunofluorescence-NBP2-42379-img0009.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0017.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0018.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human prostate shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0019.jpg)
![Immunohistochemistry-Paraffin: gp96/HSP90B1/GRP94 Antibody (CL2647) [NBP2-42379] - Staining of human skeletal muscle shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/gp96-HSP90B1-GRP94-Antibody-CL2647-Immunohistochemistry-Paraffin-NBP2-42379-img0020.jpg)
![Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Western-Blot-NB300-555-img0005.jpg "Western Blot: TRAP1 Antibody (TRAP1-6) [NB300-555] - Figure 2 illustrates IP of TRAP1 visualized by Coomassie Blue staining.")
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Analysis of TRAP1 in NCI-H460 Cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a TRAP1 monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. TRAP1 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0006.jpg)
![Immunohistochemistry-Paraffin: TRAP1 Antibody (TRAP1-6) [NB300-555] - Normal biopsies of deparaffinized Human liver tissue, dilution of 1:20.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunohistochemistry-Paraffin-NB300-555-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - Immunolocalization of TRAP1 in PC-3-M cells.](http://images.novusbio.com/fullsize/TRAP1-Antibody-TRAP1-6-Immunocytochemistry-Immunofluorescence-NB300-555-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: TRAP1 Antibody (TRAP1-6) [NB300-555] - PGE2 regulates migration & osteoclast differentiation through the G alpha s/PI3K/AKT/MAPK signaling pathways downstream of EP4. PGE2 regulates migration & osteoclast differentiation independent of beta -arrestin1 (a) & beta -arrestin2 (b) but via G alpha s (c). BMMs from the WT & littermate beta -arrestin1- & beta -arrestin2-knockout mice were used to generate osteoclasts by stimulation with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL & incubation with 100 nmol·L−1 PGE2. For G alpha s experiments, osteoclasts were generated from the GMMs of the G alpha sfl/fl controls & G alpha sfl/fl; LysM-cre (G alpha sLysM) mice. A differentiation assay (TRAP staining) (left) & the corresponding quantitative analysis (right). Scale bars, 50 μm. Error bars are the mean ± s.d. n = 3. Two-way ANOVA followed by Tukey’s t tests. Scale bars, 50 μm. d cAMP production was measured via ELISAs for osteoclasts derived from the Ep4fl/fl & Ep4LysM mice. BMMs from the two mouse strains were isolated & stimulated with 10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL to differentiate into osteoclasts & incubated with 100 nmol·L−1 PGE2 for 30 min prior to cAMP measurements. Error bars are the mean ± s.d. ***P < 0.001 by unpaired two-tailed Student’s t test. The experiment was performed with three biological replicates. e Representative images of the indicated protein expression by western blotting for osteoclasts generated using BMMs of Ep4fl/fl & Ep4LysM mice. The cells were treated either with osteoclastogenic media (10 ng·mL−1 M-CSF & 50 ng·mL−1 RANKL) alone, with PGE2 (100 nmol·L−1), or PGE2 with IBMX (1 mmol·L−1) for 3 h. The experiments were performed with three biological replicates Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35260562), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-555_mouse-monoclonal-trap1-antibody-trap1-6-310202416212330.jpg)
![Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77682] - HSP90-1A antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77682-img0005.jpg "Immunocytochemistry/Immunofluorescence: HSP90 alpha Antibody [NBP1-77682] - HSP90-1A antibody was tested in MCF-7 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).")
![Immunohistochemistry: HSP90 alpha Antibody [NBP1-77682] - Analysis of Hsp901a in human kidney carcinoma using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HSP90-alpha-Antibody-Immunohistochemistry-NBP1-77682-img0006.jpg)
![Western Blot: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Western Blot analysis of HeLa cell lysate using HSP60 Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Western-Blot-NBP2-44758-img0008.jpg "Western Blot: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Western Blot analysis of HeLa cell lysate using HSP60 Antibody (HSPD1/780)")
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Immunofluorescent staining of PFA-fixed MCF-7 cells. HSP60 Antibody (HSPD1/780) followed by goat anti-Mouse IgG-CF488 (Green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunocytochemistry-Immunofluorescence-NBP2-44758-img0006.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Renal Cell Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Immunofluorescent staining of MeOH-fixed HeLa cells. HSP60 Antibody (HSPD1/780) followed by goat anti-Mouse IgG-CF488 (green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunocytochemistry-Immunofluorescence-NBP2-44758-img0005.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Bladder Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0001.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Lung Carcinoma stained with HSP60 Monoclonal Antibody (HSPD1/780)](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0002.jpg)
![Immunohistochemistry-Paraffin: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Human Pancreas stained with HSP60 Monoclonal Antibody (HSPD1/780).](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Immunohistochemistry-Paraffin-NBP2-44758-img0003.jpg)
![Protein Array: HSP60 Antibody (HSPD1/780) [NBP2-44758] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP60 Antibody (HSPD1/780) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/HSP60-Antibody-HSPD1-780-Protein-Array-NBP2-44758-img0007.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Knockout-Validated-NBP2-76798-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Niemann-Pick Type C1/NPC1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 ug/mL of Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (Catalog # MAB10105) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Niemann-Pick Type C1/NPC1 at approximately 170-250 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 for 5 minutes. The cells were incubated with anti- Niemann-Pick C1 Antibody at 2 ug/ml for 60 minutes at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0006.jpg)
![Western Blot: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Total protein from human HeLa, Hek293, mouse 3T3 and Rat PC12 cell lines were separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NPC1 in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Western-Blot-NBP2-76798-img0005.jpg)
![Immunohistochemistry-Paraffin: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - Niemann-Pick Type C1/NPC1 was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Rabbit Anti-Human/Mouse/Rat Niemann-Pick Type C1/NPC1 Monoclonal Antibody (NBP2-76798) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic puncta.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunohistochemistry-Paraffin-NBP2-76798-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Niemann-Pick C1 Antibody (1318A) [NBP2-76798] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-Niemann-Pick C1 Antibody (1318A) at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Niemann-Pick-C1-Antibody-1318A-Immunocytochemistry-Immunofluorescence-NBP2-76798-img0004.jpg)
![Western Blot: HSP90 beta Antibody (HSP90AB1/3952) [NBP3-07186] - Western blot analysis of MCF-7 cell lysate using HSP90 beta Mouse Monoclonal Antibody (HSP90AB1/3952).](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-HSP90AB1-3952-Western-Blot-NBP3-07186-img0003.jpg "Western Blot: HSP90 beta Antibody (HSP90AB1/3952) [NBP3-07186] - Western blot analysis of MCF-7 cell lysate using HSP90 beta Mouse Monoclonal Antibody (HSP90AB1/3952).")
![Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3952) [NBP3-07186] - Formalin-fixed, paraffin-embedded human pancreas stained with HSP90 beta Mouse Monoclonal Antibody (HSP90 beta/3952).](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-HSP90AB1-3952-Immunohistochemistry-Paraffin-NBP3-07186-img0002.jpg)
![Protein Array: HSP90 beta Antibody (HSP90AB1/3952) [NBP3-07186] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP90 beta Mouse Monoclonal Antibody (HSP90 beta/3952).](http://images.novusbio.com/fullsize/HSP90-beta-Antibody-HSP90AB1-3952-Protein-Array-NBP3-07186-img0001.jpg)