| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1054AF405 | |||
| Alexa Fluor 488 | NB21-1054AF488 | |||
| Alexa Fluor 647 | NB21-1054AF647 | |||
| Alexa Fluor 700 | NB21-1054AF700 | |||
| Biotin | NB21-1054B | |||
| DyLight 405 | NB21-1054V | |||
| DyLight 488 | NB21-1054G | |||
| DyLight 550 | NB21-1054R | |||
| DyLight 650 | NB21-1054C | |||
| FITC | NB21-1054F | |||
| HRP | NB21-1054H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1054SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1143AF405 | |||
| Alexa Fluor 488 | NB21-1143AF488 | |||
| Alexa Fluor 647 | NB21-1143AF647 | |||
| Alexa Fluor 700 | NB21-1143AF700 | |||
| Biotin | NB21-1143B | |||
| DyLight 405 | NB21-1143V | |||
| DyLight 488 | NB21-1143G | |||
| DyLight 550 | NB21-1143R | |||
| DyLight 650 | NB21-1143C | |||
| FITC | NB21-1143F | |||
| HRP | NB21-1143H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1143SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-54708-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44032 | |||
| BSA Free | NBP2-52937-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| DyLight 405 | NB21-1193V | |||
| FITC | NB21-1193F | |||
![Dot Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Dot-Blot-NB21-1054-img0010.jpg "Dot Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Chromatin from one million formaldehyde cross-linked Hela cells was used with 2ug of NB21-1054 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1054-img0013.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9me3/pT6 in HeLa histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Western-Blot-NB21-1054-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Histone H3.2 K9me3 pT6 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1054-img0016.jpg)
![Western Blot: Histone H3 [p Thr6, Trimethyl Lys9] Antibody [NB21-1054] - Analysis of H3K9Me3/pT6 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Thr6--Trimethyl-Lys9]-Antibody-Western-Blot-NB21-1054-img0012.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys18] Antibody [NB21-1143] - Dot Blot analysis of Histone H3K18me3 in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys18]-Antibody-Dot-Blot-NB21-1143-img0006.jpg "Dot Blot: Histone H3 [Trimethyl Lys18] Antibody [NB21-1143] - Dot Blot analysis of Histone H3K18me3 in picomoles of peptide.")
![Western Blot: Histone H3 [Trimethyl Lys18] Antibody [NB21-1143] - WB analysis of H3.2 K18me3 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys18]-Antibody-Western-Blot-NB21-1143-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys18] Antibody [NB21-1143] - Histone H3.2 K18me3 antibody was tested at 1:50 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys18]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1143-img0012.jpg)
![Western Blot: Histone H3 [Trimethyl Lys18] Antibody [NB21-1143] - Western blot analysis of H3K18me3 in NIH/3T3 histone preps. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys18]-Antibody-Western-Blot-NB21-1143-img0008.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0016.jpg "Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody (M14), clone 5C11. Lane 1: HDAC1 transfected lysatE (55.1 KDa). Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Immunocytochemistry-Immunofluorescence-H00003065-M14-img0009.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Immunohistochemistry-Paraffin-H00003065-M14-img0010.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in PC-12 (Cat # L012V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0012.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0013.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in Raw 264.7 (Cat # L024V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0014.jpg)
![Western Blot: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of HDAC1 expression in NIH/3T3 (Cat # L018V1).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Western-Blot-H00003065-M14-img0015.jpg)
![ELISA: HDAC1 Antibody (5C11) [H00003065-M14] - Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-ELISA-H00003065-M14-img0008.jpg)
![Proximity Ligation Assay: HDAC1 Antibody (5C11) [H00003065-M14] - Analysis of protein-protein interactions between NFKB1 and HDAC1. HeLa cells were stained with anti-NFKB1 rabbit purified polyclonal 1:1200 and anti-HDAC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/HDAC1-Antibody-5C11-Proximity-Ligation-Assay-H00003065-M14-img0011.jpg)
![Western Blot: alpha Tubulin Antibody [NBP2-54708] - Analysis NBP2-54708 (A) shows similar pattern to independent antibody NBP2-54687 (B). Alpha tubulin molecular weight: 50 kDa).](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Western-Blot-NBP2-54708-img0006.jpg "<b>Independent Antibodies Validation. </b>Western Blot: alpha Tubulin Antibody [NBP2-54708] - Analysis NBP2-54708 (A) shows similar pattern to independent antibody NBP2-54687 (B). Alpha tubulin molecular weight: 50 kDa).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody [NBP2-54708] - Staining of human cell line U-2 OS shows localization to microtubules.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-54708-img0005.jpg)
![Western Blot: alpha Tubulin Antibody [NBP2-54708] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) <br/>Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)<br/><br/>Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/alpha-Tubulin-Antibody-Western-Blot-NBP2-54708-img0003.jpg)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54708] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/antibody/nbp2-54708_rabbit-polyclonal-alpha-tubulin-antibody-immunohistochemistry-paraffin-2032025122428..png)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54708] -Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-54708_rabbit-polyclonal-alpha-tubulin-antibody-immunohistochemistry-paraffin-2032025122524..png)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54708] - Staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp2-54708_rabbit-polyclonal-alpha-tubulin-antibody-immunohistochemistry-paraffin-2032025122614..png)
![Immunohistochemistry-Paraffin: alpha Tubulin Antibody [NBP2-54708] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-54708_rabbit-polyclonal-alpha-tubulin-antibody-immunohistochemistry-paraffin-203202512274..png)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human rectum and skeletal muscle tissues using NBP2-52937 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0011.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human rectum and skeletal muscle tissues using NBP2-52937 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.")
![<b>Genetic Strategies Validation and Orthogonal Strategies Validation.</b>Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HDAC1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Western-Blot-NBP2-52937-img0009.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0015.jpg)
![Western Blot: HDAC1 Antibody (CL0510) [NBP2-52937] - Analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2, mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Western-Blot-NBP2-52937-img0008.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human rectum shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0012.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human fallopian tube shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0013.jpg)
![Immunohistochemistry-Paraffin: HDAC1 Antibody (CL0510) [NBP2-52937] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/HDAC1-Antibody-CL0510-Immunohistochemistry-Paraffin-NBP2-52937-img0014.jpg)
![Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight ~16 kDa.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Western-Blot-NBP2-54611-img0001.jpg "Western Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - Western blot was performed on whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the antibody against H3K36me3. The antibody was diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight ~16 kDa.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - HeLa cells were stained with the antibody against H3K36me3 and with DAPI. Cells were fixed with 4% formaldehyde for 10 min. and blocked with PBS/Triton X-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the H3K36me3 antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Immunofluorescence-NBP2-54611-img0003.jpg)
![Chromatin Immunoprecipitation Sequencing: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - ChIP assays were performed using human K562 cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with a ChIP-seq kit, using sheared chromatin from 100,000 cells. A titration consisting of 0.2, 0.5, 1 and 2 ug of antibody per ChIP experiment was analyzed. IgG (1 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the coding region of the inactive MB gene and the Sat satellite repeat, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-Sequencing-NBP2-54611-img0015.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - ChIP assays were performed using human HeLa cells, the antibody against H3K36me3 and optimized PCR primer pairs for qPCR. ChIP was performed with an Histone ChIP-seq kit, using sheared chromatin from 1,000,000 cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the coding region of the active GAPDH and ACTB genes, used as positive controls, and for the promoter and a region located 1 kb upstream of the promoter of the GAPDH gene, used as negative controls.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Chromatin-Immunoprecipitation-NBP2-54611-img0014.jpg)
![Dot Blot: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - Histone 3 [Trimethyl Lys36] Antibody [NBP2-54611] - To test the cross reactivity of the antibody against H3K36me3, a Dot Blot analysis was performed with peptides containing other modifications or unmodified sequences of histone H3 and H4. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. The figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-3-[Trimethyl-Lys36]-Antibody-Dot-Blot-NBP2-54611-img0004.jpg)
![ELISA: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against H3K36me3. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution (Figure 3), the titer of the antibody was estimated to be 1:132,000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-ELISA-NBP2-54611-img0012.jpg)
![Protein Array: Histone H3 [Trimethyl Lys36] Antibody [NBP2-54611] - The specificity of the antibody was demonstrated by peptide array analyses on an array containing 384 peptides with different combinations of modifications from histone H3, H4, H2A and H2B. The antibody was used at a dilution of 1:10,000. The figure shows the specificity factor, calculated as the ratio of the average intensity of all spots containing the mark, divided by the average intensity of all spots not containing the mark. The peptide array analysis shows a slight cross reaction with H4K20me3 that was not observed in dot blot.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys36]-Antibody-Protein-Array-NBP2-54611-img0013.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0005.jpg "Western Blot: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-326 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunohistochemistry: GAPDH Antibody [NB300-326] - Detection of mouse GAPDH by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-GAPDH (NB300-326). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-326-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Affinity purified rabbit anti-GAPDH antibody used at 0.04 ug/ml. Theoretical molecular weight: 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0002.jpg)
![Western Blot: GAPDH Antibody [NB300-326] - Detection of GAPDH in nu/nu Mouse cells. 5% BSA in PBS, 1:5,000. Secondary: Goat anti-Rabbit IgG 1:5,000. This image was submitted via customer Review.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-326-img0003.jpg)
![Simple Western: GAPDH Antibody [NB300-326] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-326-img0001.jpg)
![Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2L6O7-Western-Blot-NBP3-15780-img0006.jpg "Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts of various cell lines, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![<b>Genetic Strategies Validation. </b>Western Blot: HDAC1 Antibody (2L6O7) [NBP3-15780] - Western blot analysis of extracts from normal (control) and HDAC1 knockout (KO) 293T cells, using HDAC1 antibody (NBP3-15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/HDAC1-Antibody-2L6O7-Knockout-Validated-NBP3-15780-img0001.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517311064.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304910.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517315592.jpg)
![Immunocytochemistry/ Immunofluorescence: HDAC1 Antibody (2L6O7) [HDAC1] - Confocal imaging of HeLa cells using [KO Validated] HDAC1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-5220251733513.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304961.jpg)
![Immunoprecipitation: HDAC1 Antibody (2L6O7) [HDAC1] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 0.5ug HDAC1 antibody . Western blot was performed from the immunoprecipitate using HDAC1 antibody at a dilition of 1:1000.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-5220251733586.jpg)
![Immunohistochemistry: HDAC1 Antibody (2L6O7) [HDAC1] - Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] HDAC1 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15780_rabbit-hdac1-mab-2l6o7-52202517304925.jpg)
![Immunoprecipitation: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Histone H3 (acetyl K9) was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32430 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32430 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-32430 IP in HeLa cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-32430 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 18 seconds; ECL](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-26820241582611.jpg "Immunoprecipitation: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Histone H3 (acetyl K9) was immunoprecipitated from 0.2 mg HeLa cell lysate with NBP3-32430 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32430 at 1/2,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.<br/><br/> Lane 1: HeLa cell lysate (input)<br/> Lane 2: NBP3-32430 IP in HeLa cell lysate<br/> Lane 3: Rabbit IgG instead of NBP3-32430 in HeLa cell lysate<br/><br/> Blocking/Dilution buffer: 5% NFDM/TBST<br/> Exposure time: 18 seconds; ECL")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and Histone H3 (NBP3-32430) / Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-26820241525102.jpg)
![Dot Blot: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Dot blot analysis of Histone H3 on different proteins with Rabbit anti-Histone H3 antibody (NBP3-32430) at 1/1,000 dilution. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution for 1 hour at room temperature.<br/><br/> Lane 1: Unmodified Histone H3 (negative)<br/> Lane 2: Mono-Methyl-Histone H3 (Lys9) (negative)<br/> Lane 3: Tri-Methyl-Histone H3 (Lys9) (negative)<br/> Lane 4: Acetyl-Histone H3 (Lys9) (positive)<br/> Lane 5: Acetyl-Histone H3 (Lys4) (negative)<br/> Lane 6: Acetyl-Histone H3 (Lys27) (negative)<br/><br/> Proteins loading: 100ng, 25ng, 5ng;<br/><br/> Blocking and dilution buffer: 5% NFDM/TBST;<br/><br/> Exposure time: 30 seconds; ECL.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-28820241459254.jpg)
![Immunohistochemistry: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Immunohistochemical analysis of paraffin-embedded human stomach tissue with Rabbit anti-Histone H3 antibody (NBP3-32430) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-298202414115442.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Immunocytochemistry analysis of C6 cells labeling Histone H3 with Rabbit anti-Histone H3 antibody (NBP3-32430) at 1/10,000 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody at 1/10,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-29202410234249.jpg)
![Flow Cytometry: Histone H3 [ac Lys9] Antibody (PSH04-47) [NBP3-32430] - Flow cytometric analysis of HeLa cells labeling Histone H3.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32430, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32430_rabbit-histone-h3-ac-lys9-mab-psh04-47-2920241645328.jpg)
![Western blot analysis on different lysates with NBP3-32430 at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 500ng/mL TSA for 4 hours cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 treated with 400nM TSA for 18 hours cell lysate Lane 5: C6 cell lysate Lane 6: C6 treated with 1μM TSA for 18 hours cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32430 at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32430_-western-blot-639204597487104361.jpg)
![Western Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H3K9/14ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Western-Blot-NBP2-59181-img0001.jpg "Western Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H3K9/14ac diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. Observed molecular weight is ~15 kDa.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP assays were performed using HeLa cells, the antibody against H3K9/14ac and optimized primer pairs for qPCR. ChIP was performed with a ChIP kit, using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control.QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0008.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP assays were performed using HeLa cells, the antibody against H3K9/14ac and optimized primer pairs for qPCR. ChIP was performed using sheared chromatin from 1.5 million cells. A titration of the antibody consisting of 1, 2, 5 and 10 ug per ChIP experiment was analysed. IgG (5 ug/IP) was used as negative IP control. QPCR was performed using primers specific for the promoter of the ACTB gene as a positive control target and for exon 2 of the MB gene as a negative control target. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA). These results confirm the observation that acetylation of H3K9/14 is present at active promoters.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0004.jpg)
![Chromatin Immunoprecipitation: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - ChIP was performed with 1 ug of the antibody against H3K9/14ac on sheared chromatin from 1 million HeLaS3 cells. IgG (2 ug/IP) was used as a negative IP control. The IP'd DNA was analysed by QPCR with optimized PCR primer pairs for the promoters of the active GAPDH and c-fos genes, used as positive control targets, and the coding region of the inactive MB gene and the Sat2 satellite repeat, used as negative control targets.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NBP2-59181-img0005.jpg)
![Dot Blot: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - A Dot Blot analysis was performed to test the cross reactivity of the antibody against H3K9/14ac with peptides containing other histone modifications and the unmodified H3K9/14 sequence. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:20,000. Figure shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-Dot-Blot-NBP2-59181-img0002.jpg)
![ELISA: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody directed against H3K9/14ac, crude serum and flow through in antigen coated wells. The antigen used was a peptie containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1:5,900.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14--ac-Lys9]-Antibody-ELISA-NBP2-59181-img0003.jpg)
![Immunofluorescence: Histone H3 [ac Lys14, ac Lys9] Antibody [NBP2-59181] - Mouse NIH3T3 cells were stained with the antibody against H3K9/14ac and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K9/14ac antibody (left) diluted 1:500 in blocking solution followed by an anti- rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys14-ac-Lys9]-Antibody-Immunofluorescence-NBP2-59181-img0006.jpg)
![Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/GAPDH-primer-Chromatin-Immunoprecipitation-NBP1-71650-img0004.jpg "Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - 2 ug of NB21-1023 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).")
![Chromatin Immunoprecipitation: GAPDH primer [NBP1-71650] - qRT-PCR standard curve generated from a serial dilution of sheared Hela chromatin.](http://images.novusbio.com/fullsize/GAPDH-primer-Chromatin-Immunoprecipitation-NBP1-71650-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Histone H3 K27me3/pS28 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28--Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1193-img0016.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Histone H3 K27me3/pS28 antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Dot Blot: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Analysis of NB21-1193 using the peptides listed above in 100, 10, and 1 picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28--Trimethyl-Lys27]-Antibody-Dot-Blot-NB21-1193-img0006.jpg)
![Chromatin Immunoprecipitation: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1193 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28--Trimethyl-Lys27]-Antibody-Chromatin-Immunoprecipitation-NB21-1193-img0010.jpg)
![Western Blot: Histone H3 [p Ser28, Trimethyl Lys27] Antibody [NB21-1193] - Tested in (1) HeLa histone prep (2) NIH-3T3 histone prep and (3) C. elegans embryo cell lysate. Observed molecular weight is ~16 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser28--Trimethyl-Lys27]-Antibody-Western-Blot-NB21-1193-img0013.jpg)