| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36468SS | |||
| Azide and BSA Free | NBP2-80770 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44038 | |||
| BSA Free | NBP2-59783-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1091AF405 | |||
| Alexa Fluor 488 | NB21-1091AF488 | |||
| Alexa Fluor 647 | NB21-1091AF647 | |||
| Alexa Fluor 700 | NB21-1091AF700 | |||
| Biotin | NB21-1091B | |||
| DyLight 405 | NB21-1091V | |||
| DyLight 488 | NB21-1091G | |||
| DyLight 550 | NB21-1091R | |||
| DyLight 650 | NB21-1091C | |||
| FITC | NB21-1091F | |||
| HRP | NB21-1091H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1091SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB500-267SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1074AF405 | |||
| Alexa Fluor 488 | NB21-1074AF488 | |||
| Alexa Fluor 647 | NB21-1074AF647 | |||
| Alexa Fluor 700 | NB21-1074AF700 | |||
| Biotin | NB21-1074B | |||
| DyLight 405 | NB21-1074V | |||
| DyLight 488 | NB21-1074G | |||
| DyLight 550 | NB21-1074R | |||
| DyLight 650 | NB21-1074C | |||
| FITC | NB21-1074F | |||
| HRP | NB21-1074H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1074SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1062AF405 | |||
| Alexa Fluor 488 | NB21-1062AF488 | |||
| Alexa Fluor 647 | NB21-1062AF647 | |||
| Alexa Fluor 700 | NB21-1062AF700 | |||
| Biotin | NB21-1062B | |||
| DyLight 405 | NB21-1062V | |||
| DyLight 488 | NB21-1062G | |||
| DyLight 550 | NB21-1062R | |||
| DyLight 650 | NB21-1062C | |||
| FITC | NB21-1062F | |||
| HRP | NB21-1062H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1062SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NB21-1002AF405 | |||
| Alexa Fluor 488 | NB21-1002AF488 | |||
| Alexa Fluor 647 | NB21-1002AF647 | |||
| Alexa Fluor 700 | NB21-1002AF700 | |||
| Biotin | NB21-1002B | |||
| DyLight 405 | NB21-1002V | |||
| DyLight 488 | NB21-1002G | |||
| DyLight 550 | NB21-1002R | |||
| DyLight 650 | NB21-1002C | |||
| FITC | NB21-1002F | |||
| HRP | NB21-1002H | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB21-1002SS | |||
![Chromatin Immunoprecipitation: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NBP2-36468 and 25 ul of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Chromatin-Immunoprecipitation-NBP2-36468-img0003.jpg "Chromatin Immunoprecipitation: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Chromatin from one million formaldehyde cross-linked HeLa cells was precipitated using 2 ug of NBP2-36468 and 25 ul of magnetic IgG beads, using standard ChIP methods. A similar sample containing no antibody was included as a negative control. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin. Representative target genes from active, inactive and heterochromatic regions of the genome show amplification, indicative of the presence of Histone H3.")
![Western Blot: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Western blot image of Anti-Histone H3 antibody (clone 1B1-B2). Whole cell protein from HeLa (lane 1), NIH-3T3 (lane 2) and PC12 (lane 3) were separated by 4-15% SDS-PAGE and transfered to PVDF membrane. The membrane was probed with purified Anti-Histone H3 antibody at 2 ug/ml and detected with an HRP conjugated anti-mouse secondary using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Western-Blot-NBP2-36468-img0002.jpg)
![Immunohistochemistry-Paraffin: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Analysis of Histone H3 in human breast cancer using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Immunohistochemistry-Paraffin-NBP2-36468-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 Antibody (1B1-B2) [NBP2-36468] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Histone H3 (1B1-B2) NBP2-36468 at a 1:200 dilution overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Immunocytochemistry-Immunofluorescence-NBP2-36468-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Histone H3 Antibody (1B1-B2) [NBP2-36468] - Analysis in Hela cells using siRNA to knockdown Pol II, controls are GAPDH and Histone H3. Theoretical molecular weight of Histone H3 is 15 kDa. Image from a verified customer review.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-1B1-B2-Western-Blot-NBP2-36468-img0009.jpg)
![Flow Cytometry: Histone H3 Antibody (1B1-B2) - BSA Free [NBP2-36468] - Analysis using Alexa Fluor (R) 647 conjugate of NBP2-36468. Jurkat cells stained with Alexa Fluor 647 conjugated Histone H3 antibody (red) or isotype control (blue). Flow cytometry image submitted by a verified customer review.](http://images.novusbio.com/fullsize/nbp2-36468_mouse-monoclonal-histone-h3-antibody-1b1-b2-17720241044623.jpg)
![Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Western-Blot-NBP2-59783-img0012.jpg "Western Blot: Lamin B1 Antibody (CL3929) [NBP2-59783] - Analysis in human cell line MCF-7.")
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human colon shows nuclear membrane positivity in glandular and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0006.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human tonsil shows strong nuclear membrane immunoreactivity in lymphoid cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0001.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human oral mucosa shows nuclear membrane positivity in the epithelial cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0002.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human prostate shows nuclear membrane immunoreactivity in glandular epithelium cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0003.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human small intestine shows nuclear membrane immunoreactivity in glandular and connective tissue cells.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0004.jpg)
![Immunohistochemistry-Paraffin: Lamin B1 Antibody (CL3929) [NBP2-59783] - Staining of human testis shows strong nuclear membrane positivity in a subset of cells in the seminiferous tubules.](http://images.novusbio.com/fullsize/Lamin-B1-Antibody-CL3929-Immunohistochemistry-Paraffin-NBP2-59783-img0005.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Detection of mouse and rat GAPDH by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GAPDH antibody NB300-325 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0006.jpg "Western Blot: GAPDH Antibody [NB300-325] - Detection of mouse and rat GAPDH by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified rabbit anti-GAPDH antibody NB300-325 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-325] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-325-img0003.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Whole cell lysate (50 ug) from HeLa (H) and 293T (50 ug) probed with GAPDH Antibody diluted at 0.04 ug/ml](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0001.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - Detection of Mouse GAPDH Whole cell lysate (5, 15 and 50 ug) from mouse NIH3T3 cells. Another Affinity purified rabbit anti-GAPDH used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-325-img0005.jpg)
![Simple Western: GAPDH Antibody [NB300-325] - Simple Western lane view shows a specific band for GAPDH in 0.05 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-325-img0004.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416212345.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416212378.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-310202416205140.jpg)
![Western Blot: GAPDH Antibody [NB300-325] - GCNY-medicated serum decreased Nrf2 levels & inflammatory cytokines of NF-kappa B & IL-6. (A) Intracellular relative ROS levels in HMC-3 cells treated with different concentrations (0, 50, 100, & 200 μm) of H2O2 for 4 h. Auto-fluo refers to the auto-fluorescence intensity value of HMC-3 cells. (B,C) The mRNA & protein levels of Nrf2 were expressed by HMC-3 treated with different concentrations of H2O2. (D) The significantly elevated whole protein level in NC + H2O2 group cells indicates an increased proliferation of HMC-3 cells compared to the NC group cells & (E) ROS levels were attenuated in cells treated by GCNY-medicated serum. (F–H) Through automated Western blot analysis, GCNY group cells expressed lower levels of Nrf2, NF-kappa s p65, pNF-kappa B p65 (ser536), & IL-6 compared with NC + H2O2 group cells. ns, no significance, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/36188472), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb300-325_rabbit-polyclonal-gapdh-antibody-31020241621812.jpg)
![Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Western-Blot-NB600-1168-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - Whole extract of HeLa cell treated with nocodazole was separated on SDS-PAGE and probed with Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG-Peroxidase and a chemiluminescent substrate.Lanes:1. Antibody dilution 0.5 ug/mL2. Antibody dilution 1 ug/mL3. Antibody dilution 2 ug/mL")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - HeLa cells were fixed and permeabilized with methanol followed by methanol:acetone. Fixed cells were stained with 5 ug/mL Monoclonal Anti-phospho-Histone H3 (pSer28), Clone: HTA28. The antibody was developed using Goat Anti-Rat IgG, FITC conjugate (green). Cells were counterstained with DAPI (blue) to stain nuclei.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Immunocytochemistry-Immunofluorescence-NB600-1168-img0007.jpg)
![Flow Cytometry: Histone H3 [p Ser28] Antibody (HTA28) [NB600-1168] - FACS profile of human leukemic cells with Anti-phospho-Histone H3.](http://images.novusbio.com/fullsize/Histone-H3-p-Ser28-Antibody-HTA28-Flow-Cytometry-NB600-1168-img0006.jpg)
![Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of HIstone H3pS10 with picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Dot-Blot-NB21-1091-img0009.jpg "Dot Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of HIstone H3pS10 with picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [p Ser10] Antibody [NB21-1091] - IF Confocal analysis of HeLa cells using Histone H3 [p Ser10] antibody (NB21-1091, 1:5). An Alexa Fluor 488-conjugated Goat to rabbit IgG was used as secondary antibody (green). Actin filaments were labeled with Alexa Fluor 568 phalloidin (red). DAPI was used to stain the cell nuclei (blue).](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1091-img0017.jpg)
![Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of Histone H3pS10 in HeLa histone prep lysates. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Western-Blot-NB21-1091-img0010.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of FFPE human testis using Histone H3 [pS10] antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclei staining of Histone H3 was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Immunohistochemistry-Paraffin-NB21-1091-img0015.jpg)
![Western Blot: Histone H3 [p Ser10] Antibody [NB21-1091] - Analysis of H3pS10 in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[p-Ser10]-Antibody-Western-Blot-NB21-1091-img0011.jpg)
![Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-324-img0009.jpg "Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH by Western Blot. Theoretical molecular weight: 36 kDa. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GAPDH antibody NB300-324 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.")
![Immunocytochemistry/Immunofluorescence: GAPDH Antibody [NB300-324] - GAPDH antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunocytochemistry-Immunofluorescence-NB300-324-img0002.jpg)
![Immunohistochemistry: GAPDH Antibody [NB300-324] - Detection of human GAPDH by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-GAPDH (NB100-79808). Detection: DAB](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-NB300-324-img0010.jpg)
![Western Blot: GAPDH Antibody [NB300-324] - Detection of Human and Mouse GAPDH, theoretical molecular weight 36 kDa. Whole cell lysate from HeLa (5, 15 and 50 mcg) and mouse NIH3T3 (M; 50 mcg). Affinity purified rabbit anti-GAPDH antibody used at 0.04 mcg/ml. Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Western-Blot-NB300-324-img0008.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded human lung carcinoma tissue sections with NB300-324 at a dilution of 1:200](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-324-img0003.jpg)
![Immunohistochemistry-Paraffin: GAPDH Antibody [NB300-324] - IHC detection of GAPDH in formalin-fixed paraffin-embedded mouse squamous cell carcinoma tissue sections with NB300-324 at a dilution of 1:200.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Immunohistochemistry-Paraffin-NB300-324-img0004.jpg)
![Simple Western: GAPDH Antibody [NB300-324] - Simple Western lane view shows a specific band for GAPDH in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. Note: band observed higher than predicted molecular weight of 36 kDa.](http://images.novusbio.com/fullsize/GAPDH-Antibody-Simple-Western-NB300-324-img0007.jpg)
![Western Blot: Histone H3 Antibody [NB500-267] - Detection of Histone H3 in lysates using NB500-267. (1) MEF and (2) HeLa. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-267-img0003.jpg "Western Blot: Histone H3 Antibody [NB500-267] - Detection of Histone H3 in lysates using NB500-267. (1) MEF and (2) HeLa. Observed molecular weight is ~15 kDa.")
![Western Blot: Histone H3 Antibody [NB500-267] - Analysis of HeLa lysates using NB500-267. Image courtesy of Gregg Semenza, (PMID: 21620138). Theoretical molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-Antibody-Western-Blot-NB500-267-img0004.jpg)
![Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Dot-Blot-NB21-1074-img0011.jpg "Dot Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9 acetyl in picomoles of peptide.")
![Chromatin Immunoprecipitation: Histone H3 [ac Lys9] Antibody [NB21-1074] - 2 ug of NB21-1074 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Chromatin-Immunoprecipitation-NB21-1074-img0014.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of H3K9ac in C. elegans embryo lysate. Observed molecular weight is ~12 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [ac Lys9] Antibody [NB21-1074] - Histone H3.2 K9ac antibody was tested at 1:100 in HeLa cells with FITC (green).](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1074-img0018.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in HeLa histone prep. Observed molecular weight is ~17 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0016.jpg)
![Western Blot: Histone H3 [ac Lys9] Antibody [NB21-1074] - Analysis of Histone H3 K9ac in NIH/3T3 histone preps. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[ac-Lys9]-Antibody-Western-Blot-NB21-1074-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16840-img0027.jpg "Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Mouse intestine. Histone H3K27me3 (Tri-methyl Lys27) stained by Histone H3K27me3 (Tri-methyl Lys27) antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunohistochemistry-Paraffin-NBP2-16840-img0026.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody U87 cells were grown under normoxic (DMEM 10% FBS, 16% O2), hypoxic (DMEM 10% FBS, 1% O2), and neurosphere conditions (DMEM/F12, B-27 supplement, growth factor (10ng/ml FGF and 20ng/ml EGF)). Cell lysate were Western blotted for H3K27me3 and Histone-H3 (loading control).](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Western-Blot-NBP2-16840-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 [Trimethyl Lys27] Antibody Confocal immunofluorescence analysis of paraformaldehyde-fixed A431, using HIST1H3A (tri-Methyl K27) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16840-img0005.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Hela xenograft, using Histon H3 (tri-Methyl K27) antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Histone-H3-[Trimethyl-Lys27]-Antibody-Immunohistochemistry-Paraffin-NBP2-16840-img0006.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (NBP2-16840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16840_rabbit-polyclonal-histone-h3-trimethyl-lys27-antibody-91020242063319.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (NBP2-16840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-16840_rabbit-polyclonal-histone-h3-trimethyl-lys27-antibody-9102024201141.jpg)
![Immunocytochemistry/ Immunofluorescence: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Histone H3 antibody detects Histone H3 protein at nucleus by immunofluorescent analysis.<br>Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.<br>Green: Histone H3 stained by Histone H3 antibody (NBP2-16840) diluted at 1:500.<br>Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp2-16840_rabbit-polyclonal-histone-h3-trimethyl-lys27-antibody-91020242021980.jpg)
![Western Blot: Histone H3 [Trimethyl Lys27] Antibody [NBP2-16840] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 antibody (NBP2-16840) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp2-16840_rabbit-polyclonal-histone-h3-trimethyl-lys27-antibody-910202420122015.jpg)
![Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1062 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg8]-Antibody-Chromatin-Immunoprecipitation-NB21-1062-img0007.jpg "Chromatin Immunoprecipitation: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Chromatin from one million formaldehyde cross-linked HeLa cells was used with 2ug of NB21-1062 and 20ul of magnetic IgG beads per immunoprecipitation. A no antibody (No Ab) control was also used. Immunoprecipitated DNA was quantified using quantitative real-time PCR and SYBR green dye, then normalized to the non-precipitated input chromatin, which is equal to one.")
![Western Blot: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Analysis of Histone H3R8me2 in: (1) HeLa Histone Prep Lysate, (2) NIH 3T3 Histone Prep Lysate, and (3) C. elegans Embryo Lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg8]-Antibody-Western-Blot-NB21-1062-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg8] Antibody [NB21-1062] - Immunofluorescence: [NB21-1062] - Histone H3.2 R8me2a antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg8]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1062-img0011.jpg)
![Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Dot-Blot-NB21-1002-img0007.jpg "Dot Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in picomoles of peptide.")
![Immunocytochemistry/Immunofluorescence: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Histone H3 [Asym-dimethyl Arg2] antibody was tested in HeLa cells with DyLight 488 (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-1002-img0006.jpg)
![Western Blot: Histone H3 [Asym-dimethyl Arg2] Antibody [NB21-1002] - Analysis of Histone H3 [Asym-dimethyl Arg2] in C. elegans embryo lysate. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/fullsize/Histone-H3-[Asym-dimethyl-Arg2]-Antibody-Western-Blot-NB21-1002-img0008.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody; alpha tubulin molecular weight: 50 kDa. Image from verified customer review.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0007.jpg "Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of Tubulin in HEK293T and NIH/3T3 cells using Tubulin antibody; alpha tubulin molecular weight: 50 kDa. Image from verified customer review.")
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-tubulin (TU-01; green) and anti-Vimentin (VI-01; red). Nucleus is stained with DAPI (blue).](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0001.jpg)
![Immunoprecipitation: alpha Tubulin Antibody (TU-01) [NB500-333] - alpha Tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Molecular weight of alpha tubulin is around 50 kDa. L = lysate IPr = immunoprecipitate (reducing conditions) IPn = immunoprecipitate (non-reducing conditions)](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunoprecipitation-NB500-333-img0006.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Analysis of alpha-tubulin (molecular weight of 50 kDa) in porcine brain lysate by antibody TU-01 biotin.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Tubulin filament in HeLa. Image from verified customer review.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0005.jpg)
![Western Blot: alpha Tubulin Antibody (TU-01) [NB500-333] - Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of lysates of various cell lines by a newly developed monoclonal antibody (B). Alpha tubulin molecular weight: 50 kDa.](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Western-Blot-NB500-333-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: alpha Tubulin Antibody (TU-01) [NB500-333] - Staining of HeLa human cervix carcinoma cell line using anti-alpha-tubulin (TU-01; red). Nucleus is stained with DAPI (blue).](http://images.novusbio.com/fullsize/Tubulin-Antibody-TU-01-Immunocytochemistry-Immunofluorescence-NB500-333-img0002.jpg)
