| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87258-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44465 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38199-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-36770 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70150 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-03895-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26590-50ul | |||
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-87258] - Staining in human liver and skeletal muscle tissues using NBP1-87258 antibody. Corresponding ABCD3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87258-img0019.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-87258] - Staining in human liver and skeletal muscle tissues using NBP1-87258 antibody. Corresponding ABCD3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-87258] - Staining of human kidney, liver, skeletal muscle and testis using Anti-ABCD3 antibody NBP1-87258 (A) shows similar protein distribution across tissues to independent antibody NBP2-38199 (B).](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87258-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody [NBP1-87258] - Staining of human cell line A-431 shows localization to peroxisomes. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-87258-img0004.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-87258] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87258-img0020.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-87258] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87258-img0016.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-87258] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87258-img0017.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-87258] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87258-img0018.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot- PMP70 Antibody [NBP1-87258] - Analysis in human cell lines RT-4 and MCF-7 using Anti-ABCD3 antibody. Corresponding ABCD3 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/antibody/nbp1-87258_rabbit-polyclonal-pmp70-antibody-western-blot-261202492347..jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in human liver and pancreas tissues. Corresponding ABCD3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0025.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in human liver and pancreas tissues. Corresponding ABCD3 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in U251 cell line showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in HeLa cell line showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human cell line A431 showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in MCF7 cell line showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (CL2524) [NBP2-36770] - Staining in U2OS cell line showing specific staining of peroxisomes in green. Microtubule-staining and nuclear probes are visualized in red and blue respectively (where available). Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunocytochemistry-Immunofluorescence-NBP2-36770-img0010.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human rectum shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0015.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human liver shows moderate granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0019.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0020.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human small intestine shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0021.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human fallopian tube shows moderate granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0023.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody (CL2524) [NBP2-36770] - Staining of human pancreas shows weak granular cytoplasmic positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/PMP70-Antibody-CL2524-Immunohistochemistry-Paraffin-NBP2-36770-img0024.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody [NBP2-38199] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-38199-img0004.jpg "Immunocytochemistry/Immunofluorescence: PMP70 Antibody [NBP2-38199] - Staining of human cell line A-431 shows localization to vesicles. Antibody staining is shown in green. Antibody staining is shown in green.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining in human liver and skeletal muscle tissues using NBP2-38199 antibody. Corresponding ABCD3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0016.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0011.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human kidney, liver, skeletal muscle and testis using Anti-ABCD3 antibody NBP2-38199 (A) shows similar protein distribution across tissues to independent antibody NBP1-87258 (B).](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0012.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0013.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0014.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP2-38199] - Staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP2-38199-img0015.jpg)
![Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Knockdown-Validated-NBP2-46476-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Western-Blot-NBP2-46476-img0004.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0003.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0001.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) [NBP2-46476] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Immunohistochemistry-NBP2-46476-img0002.jpg)
![Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Western-Blot-NBP3-16348-img0003.jpg "Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Immunofluorescence analysis of NIH-3T3 cells using PMP70 Rabbit mAb (NBP3-16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Immunocytochemistry-Immunofluorescence-NBP3-16348-img0002.jpg)
![Western Blot: PMP70 Antibody (3V4K3) [NBP3-16348] - Western blot analysis of extracts of various cell lines, using PMP70 Rabbit mAb (NBP3-16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Western-Blot-NBP3-16348-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Immunofluorescence analysis of HeLa cells using PMP70 Rabbit mAb (NBP3-16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-3V4K3-Immunocytochemistry-Immunofluorescence-NBP3-16348-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Confocal imaging of C2C12 cells using PMP70 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16348_rabbit-pmp70-mab-3v4k3-52202511402772.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (3V4K3) [NBP3-16348] - Confocal imaging of HeLa cells using PMP70 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L).The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green).DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16348_rabbit-pmp70-mab-3v4k3-522025125489.jpg)
![Western Blot: ELOVL4 Antibody [NBP3-03895] - Analysis of A-431, using ELOVL4 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/ELOVL4-Antibody-Western-Blot-NBP3-03895-img0004.jpg "Western Blot: ELOVL4 Antibody [NBP3-03895] - Analysis of A-431, using ELOVL4 antibody at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Western Blot: ELOVL4 Antibody [NBP3-03895] -analysis of lysates from A-431 cells using ELOVL4 Rabbit pAb at 1:600 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time:30s.](http://images.novusbio.com/fullsize/antibody/nbp3-03895_rabbit-polyclonal-elovl4-antibody-western-blot-4122024101636..png)
![Immunocytochemistry/ Immunofluorescence: ELOVL4 Antibody - Azide and BSA Free [ELOVL4] - Immunofluorescence analysis of A-431 cells using ELOVL4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03895_rabbit-polyclonal-elovl4-antibody-5220251633416.jpg)
![Immunocytochemistry/ Immunofluorescence: ELOVL4 Antibody - Azide and BSA Free [ELOVL4] - Immunofluorescence analysis of NIH/3T3 cells using ELOVL4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03895_rabbit-polyclonal-elovl4-antibody-52202516321487.jpg)
![Immunocytochemistry/ Immunofluorescence: ELOVL4 Antibody - Azide and BSA Free [ELOVL4] - Immunofluorescence analysis of C6 cells using ELOVL4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03895_rabbit-polyclonal-elovl4-antibody-5220251636317.jpg)
![Immunocytochemistry/ Immunofluorescence: ELOVL4 Antibody - Azide and BSA Free [ELOVL4] - Immunofluorescence analysis of L929 cells using ELOVL4 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03895_rabbit-polyclonal-elovl4-antibody-5220251628323.jpg)
![Western Blot: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Western-Blot-NBP2-70150-img0001.jpg "Western Blot: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ABCD1.")
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0004.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0002.jpg)
![Immunohistochemistry: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70150-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Knockout Validated: ABCD1 Antibody (OTI2C12) - Azide and BSA Free [NBP2-70150] - Western blot shows lysates of TIME human endothelial parental cell line and ABCD1 knockout (KO) TIME cell line. PVDF membrane was probed with 1:2000 of Mouse Anti-Human ABCD1 Monoclonal Antibody (Catalog # NBP2-46476) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #HAF018). Specific band was detected for ABCD1 at approximately 75 kDa (as indicated) in the parental TIME cell line, but is not detectable in the knockout TIME cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/ABCD1-Antibody-OTI2C12-Azide-and-BSA-Free-Knockout-Validated-NBP2-70150-img0005.jpg)
![Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-288202412461658.jpg "Western Blot: PMP70 Antibody (PSH03-27) [NBP3-32834] - Western blot analysis of PMP70 on different lysates with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: SH-SY5Y cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 76 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32834) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32834) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-298202414333688.jpg)
![Immunocytochemistry/ Immunofluorescence: PMP70 Antibody (PSH03-27) [NBP3-32834] - Immunocytochemistry analysis of HepG2 cells labeling PMP70 with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PMP70 antibody (NBP3-32834) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-29202410334056.jpg)
![Flow Cytometry: PMP70 Antibody (PSH03-27) [NBP3-32834] - Flow cytometric analysis of NIH/3T3 cells labeling PMP70. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32834, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32834_rabbit-pmp70-mab-psh03-27-2920241637212.jpg)
![Immunohistochemistry: FADS1 Antibody (7A10) [NBP3-26590] - Image of FADS1 Antibody (7A10) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26590_rabbit-fads1-mab-7a10-262202414471574.jpg "Immunohistochemistry: FADS1 Antibody (7A10) [NBP3-26590] - Image of FADS1 Antibody (7A10) diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Western Blot: FADS1 Antibody (7A10) [NBP3-26590] - Positive Western Blot detected in: MCF-7 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate. All lanes: FADS1 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 43 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26590_rabbit-fads1-mab-7a10-28220241152510.jpg)
![Immunocytochemistry/Immunofluorescence: FADS1 Antibody (7A10) [NBP3-26590] - Staining of HepG2 cell with FADS1 Antibody (7A10) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 565-conjugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/nbp3-26590_rabbit-fads1-mab-7a10-282202412475112.jpg)
![Flow Cytometry: FADS1 Antibody (7A10) [NBP3-26590] - Overlay Peak curve showing HepG2 cells stained with FADS1 Antibody (7A10) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26590_rabbit-fads1-mab-7a10-282202414183689.jpg)
![Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - Staining of Human Testis with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/PMP70-Antibody-Immunohistochemistry-Paraffin-NBP1-20871-img0001.jpg "Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - Staining of Human Testis with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.")
![Flow Cytometry: PMP70 Antibody [NBP1-20871] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/PMP70-Antibody-Flow-Cytometry-NBP1-20871-img0003.jpg)