| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92683SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38622-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72582 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71422 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72584 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84687-25ul | |||
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunocytochemistry-Immunofluorescence-NBP2-67295-img0010.jpg "Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Analysis of Hela cells with ALP antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Flow-Cytometry-NBP2-67295-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunocytochemistry-Immunofluorescence-NBP2-67295-img0003.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0011.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minut](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0012.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0013.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] -Analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody at 1/5,000 dilution.Lane 1: Saos-2 cell lysate (15ug/Lane)Lane 2: HeLa cell lysate (15 ug/Lane)Lane 3: A549 cell lysate (15 ug/Lane)Lane 4: Mouse liver tissue lysate (20 ug/Lane)Lane 5: Rat liver tissue lysate (20 ug/Lane)Predicted band size: 57 kDaObserved band size: 75 kDaExposure time: 24 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67295_rabbit-monoclonal-alkaline-phosphatase-tissue-non-specific-antibody-sa40-00-western-blot-1482024111635..jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0005.jpg "Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of HEK293 cell lysates using Adenylate Cyclase 3 antibody, dilution 1:2000 (Green): [1] protein standard, [2] non-transfected HEK293 cells, and [3] HEK293 cells transfected with an expression construct containing a Myc-DDK tagged full length human Adenylate Cyclase 3 cDNA. The strong band at about 130kDa in the transfected cells demonstrates overexpression of the human Adenylate Cyclase 3 protein, and the bands over 250kDa presumably correspond to either glycosylated or aggregated forms of Adenylate Cyclase 3. The blot was simultaneously probed with mouse GAPDH mAb, dilution 1:5000 (Red), which reveals the single band at ~37kDa in both transfected and non-transfected cells.")
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Analysis of rat hippocampus stained with NBP1-92683 antibody to adenylate cyclase III in red and mouse antibody to MECP2 in green, DNA is revealed with DAPI in blue. This is a stacked confocal image made with a 100X objective lens. The ACIII antibody reveals neuronal cilia while the MECP2 antibody reveals the nuclei of certain neurons to a variable degree.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0004.jpg)
![Western Blot: Adenylate Cyclase 3 Antibody [NBP1-92683] - Western blot of rat olfactory epithelium (OE) and frontal cortex (FC). NBP1-92683 stains a band at about 200kDa in olfactory epithelium which is rich in cilia. Fewer cilia are found in frontal cortex, and the protein is less heavily glycosylated, and a less prominent band is seen at about 160kDa. Data generated in the laboratory of Matt Sarkisian in the University of Florida.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Western-Blot-NBP1-92683-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Adenylate Cyclase 3 Antibody [NBP1-92683] - Mixed neuron-glial cultures stained with Adenylate Cyclase 3 antibody (NBP1-92683, red) and Alpha Fodrin antibody (NBP1-92689, green). Note the strong and clean staining of neuronal cilia. Since Alpha Fodrin is specific for neurons in the CNS, the glial cells in this culture are not recognized by this antibody.](http://images.novusbio.com/fullsize/Adenylate-Cyclase-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-92683-img0003.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well. Data courtesy of the Innovators Program.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0007.jpg "Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of 8.4ug of rabbit subcutaneous white adipose tissue (scWAT), gonadal white adipose tissue (gWAT), perirenal white adipose tissue (pWAT), tibia marrow adipose tissue (tibMAT), tibia red marrow (tibRM), and 3T3-L1 positive control adipocyte lysates per well. Data courtesy of the Innovators Program.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Effect of whey peptides on 3T3-L1 adipocyte differentiation. 3T3-L1 preadipocytes were differentiated in the presence of 2.5 mg/mL WPI (whey protein isolate), WPH (whey peptide hydrolysate), BPI (BSA protein isolate) or BPH (BSA peptide hydrolysate). Immunoblotting and densitometric analysis of (A,D) adiponectin (n = 6). mRNA levels of (F) adiponectin (n = 8) were determined. * p < 0.05, **** p < 0.0001 vs. protein isolate controls. PS, protein stain. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32041341) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis of Adiponectin was performed using 10% confluent 3T3-L1 cells differentiated with Adipogenesis supplement for 5 days.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunocytochemistry-Immunofluorescence-NBP2-22450-img0011.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis was performed on biopsies of deparaffinized Human skin tissue.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunohistochemistry-Paraffin-NBP2-22450-img0010.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Analysis was performed using 10 ul of conditioned media of Hep G2 cell line (lane 1).](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Western-Blot-NBP2-22450-img0009.jpg)
![Immunohistochemistry: Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Biopsies of deparaffinized Human skin tissue.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-19F1-Immunohistochemistry-NBP2-22450-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Mouse Monoclonal Adiponectin/Acrp30 Antibody (19F1) [NBP2-22450] - Adipocytes isolated from the pelvic area of the male mouse. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-22450_mouse-monoclonal-adiponectin-acrp30-antibody-19f1-immunocytochemistry-immunofluorescence-1111202494957..jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP2-38622 (A) shows similar protein distribution across tissues to independent antibody NBP1-84687 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0012.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP2-38622-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0014.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0015.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human pancreas shows weak to moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0018.jpg)
![Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Western-Blot-NBP2-67129-img0011.jpg "Western Blot: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Western blot analysis of S1P1/EDG-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SH-SY5Y cell lysate Lane 2: HepG2 cell lysate")
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0009.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse heart tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0008.jpg)
![Flow Cytometry: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of Jurkat cells with EDG1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Flow-Cytometry-NBP2-67129-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Staining EDG1 in HUVEC cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunocytochemistry-Immunofluorescence-NBP2-67129-img0003.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0004.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human kidney tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0005.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded human liver tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0006.jpg)
![Immunohistochemistry-Paraffin: S1P1/EDG-1 Antibody (JM10-66) [NBP2-67129] - Analysis of paraffin-embedded mouse brain tissue using anti-EDG1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/S1P1-EDG-1-Antibody-JM10-66-Immunohistochemistry-Paraffin-NBP2-67129-img0007.jpg)
![Western Blot: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mannose Phosphate Isomerase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Mannose Phosphate Isomerase.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Western-Blot-NBP2-02043-img0002.jpg "Western Blot: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mannose Phosphate Isomerase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Mannose Phosphate Isomerase.")
![Immunocytochemistry/Immunofluorescence: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Mannose Phosphate Isomerase.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Immunocytochemistry-Immunofluorescence-NBP2-02043-img0009.jpg)
![Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - Staining of paraffin-embedded Human prostate tissue using anti-Mannose Phosphate Isomerase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Immunohistochemistry-Paraffin-NBP2-02043-img0008.jpg)
![Flow Cytometry: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Mannose Phosphate Isomerase antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Flow-Cytometry-NBP2-02043-img0001.jpg)
![Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-Mannose Phosphate Isomerase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Immunohistochemistry-Paraffin-NBP2-02043-img0003.jpg)
![Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Mannose Phosphate Isomerase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Immunohistochemistry-Paraffin-NBP2-02043-img0004.jpg)
![Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Mannose Phosphate Isomerase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Immunohistochemistry-Paraffin-NBP2-02043-img0005.jpg)
![Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Mannose Phosphate Isomerase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Immunohistochemistry-Paraffin-NBP2-02043-img0006.jpg)
![Immunohistochemistry-Paraffin: Mannose Phosphate Isomerase Antibody (1C7) [NBP2-02043] - Staining of paraffin-embedded Human pancreas tissue using anti-Mannose Phosphate Isomerase mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-1C7-Immunohistochemistry-Paraffin-NBP2-02043-img0007.jpg)
![Western Blot: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adiponectin.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Western-Blot-NBP2-01418-img0019.jpg "Western Blot: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Adiponectin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Adiponectin.")
![Immunocytochemistry/Immunofluorescence: Adiponectin/Acrp30 Antibody (OTI1H1) [NBP2-01418] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Adiponectin.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunocytochemistry-Immunofluorescence-NBP2-01418-img0011.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Human liver tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0018.jpg)
![Flow Cytometry: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Adiponectin antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Flow-Cytometry-NBP2-01418-img0010.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0012.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0014.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0015.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Human breast tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0016.jpg)
![Immunohistochemistry-Paraffin: Adiponectin/Acrp30 Antibody (1H1) [NBP2-01418] - Staining of paraffin-embedded Human Kidney tissue using anti-Adiponectin mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Adiponectin-Acrp30-Antibody-1H1-Immunohistochemistry-Paraffin-NBP2-01418-img0017.jpg)
![Western Blot: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mannose Phosphate.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Western-Blot-NBP2-45834-img0015.jpg "Western Blot: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mannose Phosphate.")
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Human breast tissue . (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0013.jpg)
![Flow Cytometry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Hela cells, using MPI antibody (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Flow-Cytometry-NBP2-45834-img0001.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Human tonsil within the normal limits using MPI mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0002.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0003.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0004.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0005.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0006.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0007.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0008.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0009.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0010.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0011.jpg)
![Immunohistochemistry: Mannose Phosphate Isomerase Antibody (OTI4G7) [NBP2-45834] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/Mannose-Phosphate-Isomerase-Antibody-OTI4G7-Immunohistochemistry-NBP2-45834-img0012.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Western-Blot-NBP2-67580-img0004.jpg "Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Tryptophan Hydroxylase 1/TPH1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HL-60 cell lysate Lane 2: Hela cell lysate")
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-Tryptophan Hydroxylase/TPH-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0005.jpg)
![Flow Cytometry: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of Hela cells with TPH1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Flow-Cytometry-NBP2-67580-img0001.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded mouse cerebellum tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0002.jpg)
![Immunohistochemistry-Paraffin: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of paraffin-embedded rat brain tissue using anti-TPH1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tryptophan-Hydroxylase-1-TPH-1-Antibody-SC53-07-Immunohistochemistry-Paraffin-NBP2-67580-img0003.jpg)
![Western Blot: Tryptophan Hydroxylase 1/TPH-1 Antibody (SC53-07) [NBP2-67580] - Analysis of TPH1 on different lysates with Rabbit anti-TPH1 antibody at 1/1,000 dilution.Lane 1: MDA-MB-231-si NT cell lysateLane 2: MDA-MB-231-si TPH1 cell lysateLysates/proteins at 10 ug/Lane.Predicted band size: 51 kDaObserved band size: 72 kDaExposure time: 3 minutes;4-20% SDS-PAGE gel.NBP2-67580 was shown to specifically react with TPH1 in MDA-MB-231-si NT cells. Weakened band was observed when MDA-MB-231-si TPH1 sample was tested. MDA-MB-231-si NT and Hela-si TPH1 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1/1,000) and Loading control antibody (Rabbit anti-GAPDH, (1/10,000) were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-rabbit IgG-HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67580_rabbit-monoclonal-tryptophan-hydroxylase-1-tph-1-antibody-sc53-07-western-blot-462024131133..jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249342724.jpg "Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249422658.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249413360.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249383187.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249362716.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249413379.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249403843.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP1-84687-img0005.jpg "Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0020.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows strong cytoplasmic positivity in subset of cells in tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0007.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0008.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0011.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0012.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0013.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP1-84687 (A) shows similar protein distribution across tissues to independent antibody NBP2-38622 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP1-84687 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0018.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human pancreas shows moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0019.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0021.jpg)