| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-80607 | |||
| BSA Free | NB100-1830SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38622-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 405 | NLS142AF405 | |||
| Alexa Fluor 488 | NLS142AF488 | |||
| Alexa Fluor 647 | NLS142AF647 | |||
| Alexa Fluor 700 | NLS142AF700 | |||
| Biotin | NLS142B | |||
| DyLight 405 | NLS142V | |||
| DyLight 488 | NLS142G | |||
| DyLight 550 | NLS142R | |||
| DyLight 650 | NLS142C | |||
| FITC | NLS142F | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84687-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41271-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92808-0.02ml | |||
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0012.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Detection of Calcium Sensing Receptor in transfected 293 cell lysate using NB 100-1830. ECL exposure, 5 minutes. In this photo, the protein has aggregated and thus the higher MW band as expected.")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Analysis of Calcium Sensing Receptor expression in rat kidney tissue.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Western-Blot-NB100-1830-img0011.jpg)
![Simple Western: Calcium-sensing R/CaSR Antibody (HL 1499) [NB100-1830] - Image shows a specific band for Calcium Sensing Receptor in 0.5 mg/mL of HEK293 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-HL-1499-Simple-Western-NB100-1830-img0010.jpg)
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - U2OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with antibody at 5 ug/mL overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241003248.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on Hek293 cells with Calcium-sensing R/CaSR Antibody (HL 1499) NB100-1830PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-1772024959417.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Analysis of FFPE human brain using Calcium-sensing R/CaSR antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxid](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720241044639.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - An intracellular stain was performed on HEK293 cells with Calcium-sensing R/CaSR Antibody [HL 1499] NB100-1830F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to FITC.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-177202495941.jpg)
![Flow Cytometry: Calcium-sensing R/CaSR Antibody (HL 1499) - BSA Free [NB100-1830] - Intracellular flow cytometric staining of 1 x 10^6 CHO (A) and HEK-293 (B) cells using calcium sensing receptor antibody (dark blue). Isotype control shown in orange. Antibody at 1 ug/1 x 10^6 cells.](http://images.novusbio.com/fullsize/nb100-1830_mouse-monoclonal-calcium-sensing-r-casr-antibody-hl-1499-17720249362617.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP2-38622 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP2-38622 (A) shows similar protein distribution across tissues to independent antibody NBP1-84687 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0012.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP2-38622-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0014.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0015.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human pancreas shows weak to moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP2-38622] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP2-38622-img0018.jpg)
![Immunohistochemistry: GPCR GPR39 Antibody [NLS142] - IHC analysis of GPR39 in human brain using Fast Red with hematoxylin counterstain.](http://images.novusbio.com/fullsize/GPCR-GPR39-Antibody-Immunohistochemistry-NLS142-img0005.jpg "Immunohistochemistry: GPCR GPR39 Antibody [NLS142] - IHC analysis of GPR39 in human brain using Fast Red with hematoxylin counterstain.")
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249342724.jpg "Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249422658.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249413360.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249383187.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249362716.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249413379.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody (HL2357) - Azide and BSA Free [NBP3-25393] - Calcium Sensing Receptor antibody [HL2357] detects Calcium Sensing Receptor protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Calcium Sensing Receptor stained by Calcium Sensing Receptor antibody [HL2357] (NBP3-25393) diluted at 1:400. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25393_rabbit-calcium-sensing-r-casr-mab-hl2357-azide-bsa-free-2120249403843.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Western-Blot-NBP1-84687-img0005.jpg "Western Blot: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in control (vector only transfected HEK293T lysate) and CASR over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0020.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows strong cytoplasmic positivity in subset of cells in tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0006.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows high expression.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0007.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0008.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0011.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human liver.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0012.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0013.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle using Anti-CASR antibody NBP1-84687.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0014.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney, liver, pancreas and parathyroid gland using Anti-CASR antibody NBP1-84687 (A) shows similar protein distribution across tissues to independent antibody NBP2-38622 (B).](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0015.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Analysis in human parathyroid gland and skeletal muscle tissues using NBP1-84687 antibody. Corresponding CASR RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0016.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0017.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human kidney shows moderate membranous positivity in cells in distal tubules.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0018.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human pancreas shows moderate membranous positivity in islets of Langerhans.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0019.jpg)
![Immunohistochemistry-Paraffin: Calcium-sensing R/CaSR Antibody [NBP1-84687] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Calcium-sensing-R-CaSR-Antibody-Immunohistochemistry-Paraffin-NBP1-84687-img0021.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunohistochemistry of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120241943225.jpg "Immunohistochemistry: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunohistochemistry of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 5 ug/mL.")
![Immunocytochemistry/ Immunofluorescence: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Immunofluorescence of Calcium-sensing R/CaSR in human kidney tissue with Calcium-sensing R/CaSR antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120241928876.jpg)
![Western Blot: Calcium-sensing R/CaSR Antibody - BSA Free [NBP2-41271] - Western blot analysis of Calcium-sensing R/CaSR in EL4 cell lysate with Calcium-sensing R/CaSR antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp2-41271_rabbit-polyclonal-calcium-sensing-r-casr-antibody-271120242092044.gif)
![Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-278202416331274.jpg "Western Blot: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Western blot analysis of Calcium-sensing R/CaSR on different lysates with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate (no heat) Lane 3: Rat kidney tissue lysate (no heat) Lysates/proteins at 40 ug/Lane. Predicted band size: 121 kDa Observed band size: 160 kDa Exposure time: 1 minute 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32111) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32111) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-298202414333644.jpg)
![Immunohistochemistry: Calcium-sensing R/CaSR Antibody (PSH03-47) [NBP3-32111] - Immunofluorescence analysis of paraffin-embedded mouse kidney tissue labeling Calcium-sensing R/CaSR with Rabbit anti-Calcium-sensing R/CaSR antibody (NBP3-32111) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32111, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32111_rabbit-calcium-sensing-r-casr-mab-psh03-47-29820241492610.jpg)
![Immunohistochemistry-Paraffin: GPR39 Antibody [NBP3-13032] - GPR39 antibody detects GPR39 protein at cell membrane in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. GPR39 antibody (NBP3-13032) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GPR39-Antibody-Immunohistochemistry-Paraffin-NBP3-13032-img0002.jpg "Immunohistochemistry-Paraffin: GPR39 Antibody [NBP3-13032] - GPR39 antibody detects GPR39 protein at cell membrane in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. GPR39 antibody (NBP3-13032) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: GPR39 Antibody [NBP3-13032] - GPR39 antibody detects GPR39 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.GPR39 stained by GPR39 antibody (NBP3-13032) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-13032_rabbit-gpr39-pab-91020242001042.jpg)
![Western Blot: Cytochrome P450 2C18 Antibody [NBP2-92808] - Analysis of extracts of various cell lines, using Cytochrome P450 2C18 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C18-Antibody-Western-Blot-NBP2-92808-img0003.jpg "Western Blot: Cytochrome P450 2C18 Antibody [NBP2-92808] - Analysis of extracts of various cell lines, using Cytochrome P450 2C18 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
