| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89209-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57753-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84791-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48704-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74173 | |||
![Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-288202412442940.jpg "Western Blot: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Western blot analysis of SEC14L2 on different lysates with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/1,000 dilution. Lane 1: Mouse liver tissue lysate Lane 2: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 46 kDa Observed band size: 46 kDa Exposure time: 16 seconds. 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32935) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32935) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-298202414262627.jpg)
![Immunocytochemistry/ Immunofluorescence: SEC14L2 Antibody (JE61-74) [NBP3-32935] - Immunocytochemistry analysis of A549 cells labeling SEC14L2 with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-SEC14L2 antibody (NBP3-32935) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32935_rabbit-sec14l2-mab-je61-74-29202410234211.jpg)
![Western Blot: PON1 Antibody (JE30-90) [NBP3-32843] - Western blot analysis of PON1 on different lysates with Rabbit anti-PON1 antibody (NBP3-32843) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32843) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-288202412461639.jpg "Western Blot: PON1 Antibody (JE30-90) [NBP3-32843] - Western blot analysis of PON1 on different lysates with Rabbit anti-PON1 antibody (NBP3-32843) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Mouse liver tissue lysate Lane 3: Rat liver tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32843) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PON1 Antibody (JE30-90) [NBP3-32843] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-PON1 antibody (NBP3-32843) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32843) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-298202414304136.jpg)
![Flow Cytometry: PON1 Antibody (JE30-90) [NBP3-32843] - Flow cytometric analysis of HepG2 cells labeling PON1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32843, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32843_rabbit-pon1-mab-je30-90-29202416453293.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-278202416285219.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Western blot analysis of 5'-Nucleotidase/CD73 on different lysates with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/2,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: U-87 MG cell lysate (no heat) Lysates/proteins at 30 ug/Lane. Predicted band size: 63 kDa Observed band size: 70 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32166) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunohistochemical analysis of paraffin-embedded NCI-H441 xenograft tissue with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32166) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-298202414304184.jpg)
![Immunocytochemistry/ Immunofluorescence: 5'-Nucleotidase/CD73 Antibody (A6G2-R) [NBP3-32166] - Immunocytochemistry analysis of U-87 MG cells labeling 5'-Nucleotidase/CD73 with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-5'-Nucleotidase/CD73 antibody (NBP3-32166) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32166_mouse-5-nucleotidase-cd73-mab-a6g2-r-2920241192315.jpg)
![Western Blot: PON1 Antibody [NBP2-19893] - . Human tissue extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PON1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/PON1-Antibody-Western-Blot-NBP2-19893-img0002.jpg "Western Blot: PON1 Antibody [NBP2-19893] - . Human tissue extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with PON1 antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: PON1 Antibody [NBP2-19893] - Analysis of mouse liver. PON1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/PON1-Antibody-Immunohistochemistry-Paraffin-NBP2-19893-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Apolipoprotein A-II/ApoA2 Antibody (1H6) [H00000336-M03] - Analysis of monoclonal antibody to APOA2 on HeLa cell. Antibody concentration 10 ug/ml](http://images.novusbio.com/fullsize/Apolipoprotein-A-II-ApoA2-Antibody-1H6-Immunocytochemistry-Immunofluorescence-H00000336-M03-img0008.jpg "Immunocytochemistry/Immunofluorescence: Apolipoprotein A-II/ApoA2 Antibody (1H6) [H00000336-M03] - Analysis of monoclonal antibody to APOA2 on HeLa cell. Antibody concentration 10 ug/ml")
![Immunohistochemistry-Paraffin: Apolipoprotein A-II/ApoA2 Antibody (1H6) [H00000336-M03] - Analysis of monoclonal antibody to APOA2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Apolipoprotein-A-II-ApoA2-Antibody-1H6-Immunohistochemistry-Paraffin-H00000336-M03-img0009.jpg)
![ELISA: Apolipoprotein A-II/ApoA2 Antibody (1H6) [H00000336-M03] - Detection limit for recombinant GST tagged APOA2 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Apolipoprotein-A-II-ApoA2-Antibody-1H6-ELISA-H00000336-M03-img0004.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody [NBP1-89209] - Staining in human prostate and skeletal muscle tissues using anti-PGM3 antibody. Corresponding PGM3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PGM3-Antibody-Immunohistochemistry-Paraffin-NBP1-89209-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PGM3 Antibody [NBP1-89209] - Staining in human prostate and skeletal muscle tissues using anti-PGM3 antibody. Corresponding PGM3 RNA-seq data are presented for the same tissues.")
![<b>Independent Antibodies Validation and Orthogonal Strategies Validation.</b>Western Blot: PGM3 Antibody [NBP1-89209] - Analysis using Anti-PGM3 antibody NBP1-89209 (A) shows similar pattern to independent antibody NBP1-89208 (B).](http://images.novusbio.com/fullsize/PGM3-Antibody-Western-Blot-NBP1-89209-img0009.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody [NBP1-89209] - Staining of human prostate shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PGM3-Antibody-Immunohistochemistry-Paraffin-NBP1-89209-img0006.jpg)
![Immunohistochemistry-Paraffin: PGM3 Antibody [NBP1-89209] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize/PGM3-Antibody-Immunohistochemistry-Paraffin-NBP1-89209-img0007.jpg)
![PMM2/Phosphomannomutase 2 Antibody [NBP2-57753] - Western blot -Analysis using Anti-PMM2 antibody NBP2-57753 (A) shows similar pattern to independent antibody (B).](http://images.novusbio.com/fullsize/antibody/nbp2-57753_rabbit-polyclonal-pmm2-phosphomannomutase-2-antibody-western-blot-1942023123616.jpg "<b>Independent Antibodies Validation. </b>PMM2/Phosphomannomutase 2 Antibody [NBP2-57753] - Western blot -Analysis using Anti-PMM2 antibody NBP2-57753 (A) shows similar pattern to independent antibody (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PMM2/Phosphomannomutase 2 Antibody [NBP2-57753] - Staining of human cell line SH-SY5Y shows localization to nucleus & cytosol.](http://images.novusbio.com/fullsize/PMM2-Phosphomannomutase-2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57753-img0001.jpg)
![Immunohistochemistry-Paraffin: PMM2/Phosphomannomutase 2 Antibody [NBP2-57753] - Staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PMM2-Phosphomannomutase-2-Antibody-Immunohistochemistry-Paraffin-NBP2-57753-img0003.jpg)
![Immunohistochemistry-Paraffin: PON1 Antibody [NBP1-84791] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/PON1-Antibody-Immunohistochemistry-Paraffin-NBP1-84791-img0002.jpg "Immunohistochemistry-Paraffin: PON1 Antibody [NBP1-84791] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.")
![Western Blot: DNAJC3 Antibody [NBP2-48704] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/DNAJC3-Antibody-Western-Blot-NBP2-48704-img0003.jpg "Western Blot: DNAJC3 Antibody [NBP2-48704] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4")
![Immunocytochemistry/Immunofluorescence: DNAJC3 Antibody [NBP2-48704] - Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.](http://images.novusbio.com/fullsize/DNAJC3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-48704-img0005.jpg)
![Immunohistochemistry-Paraffin: DNAJC3 Antibody [NBP2-48704] - Staining of human thyroid gland shows cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/DNAJC3-Antibody-Immunohistochemistry-Paraffin-NBP2-48704-img0004.jpg)
![Western Blot: PTPN11/SHP2 Antibody (3F2) [NBP2-03063] Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN11/SHP2 monoclonal antibody.](http://images.novusbio.com/fullsize/PTPN11-SHP2-Antibody-3F2-Western-Blot-NBP2-03063-img0004.jpg "Western Blot: PTPN11/SHP2 Antibody (3F2) [NBP2-03063] Analysis of extracts (35ug) from 9 different cell lines by using anti-PTPN11/SHP2 monoclonal antibody.")
![Flow Cytometry: PTPN11/SHP2 Antibody (3F2) [NBP2-03063] - Analysis of Jurkat cells, using anti-PTPN11/SHP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PTPN11-SHP2-Antibody-3F2-Flow-Cytometry-NBP2-03063-img0002.jpg)
![Western Blot: PTPN11/SHP2 Antibody (3F2) [NBP2-03063] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PTPN11/SHP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PTPN11/SHP2.](http://images.novusbio.com/fullsize/PTPN11-SHP2-Antibody-3F2-Western-Blot-NBP2-03063-img0003.jpg)
![Flow Cytometry: PTPN11/SHP2 Antibody (3F2) [NBP2-03063] - Analysis of Hela cells, using anti-PTPN11/SHP2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/PTPN11-SHP2-Antibody-3F2-Flow-Cytometry-NBP2-03063-img0001.jpg)
![Western Blot: SEC14L2 Antibody [NBP2-15112] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Western-Blot-NBP2-15112-img0002.jpg "Western Blot: SEC14L2 Antibody [NBP2-15112] - Sample (30 ug of whole cell lysate) A: U87-MG 10% SDS PAGE, antibody diluted at 1:500.")
![Immunohistochemistry-Paraffin: SEC14L2 Antibody [NBP2-15112] - Paraffin-embedded H1299 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SEC14L2-Antibody-Immunohistochemistry-Paraffin-NBP2-15112-img0001.jpg)
![SEC14L2 antibody [N1C1] detects SEC14L2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SEC14L2 protein stained by SEC14L2 antibody [N1C1] (NBP2-15112) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp2-15112_rabbit-polyclonal-sec14l2-antibody-immunocytochemistry-immunofluorescence-261120251849136.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Western-Blot-NBP1-32951-img0006.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Sample (30 ug of whole cell lysate) A: H1299 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - MDAMB231 xenograft, using CD73 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/5'-Nucleotidase-CD73-Antibody-Immunohistochemistry-Paraffin-NBP1-32951-img0005.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: 5'-Nucleotidase/CD73 Antibody [NBP1-32951] - Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD73 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32951_rabbit-polyclonal-5-nucleotidase-cd73-antibody-1610202419405720.jpg)