| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92396-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47908-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15122-100ul | |||
| BSA Free | NBP3-15122-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59338-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89374-50ul | |||
![Western Blot: Cysteinyl Leukotriene R1/CysLTR1 Antibody [NBP2-92396] - Analysis of extracts of various cell lines, using Cysteinyl Leukotriene R1/CysLTR1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.](http://images.novusbio.com/fullsize/Cysteinyl-Leukotriene-R1-CysLTR1-Antibody-Western-Blot-NBP2-92396-img0001.jpg "Western Blot: Cysteinyl Leukotriene R1/CysLTR1 Antibody [NBP2-92396] - Analysis of extracts of various cell lines, using Cysteinyl Leukotriene R1/CysLTR1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 3s.")
![Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody - BSA Free [NB110-40449] - Analysis of anti-SPHK1 antibody with human adrenal, pheochromocytes at concentration 10 ug/ml.](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody---BSA-Free-Immunohistochemistry-Paraffin-NB110-40449-img0001.jpg "Immunohistochemistry-Paraffin: Sphingosine Kinase 1/SPHK1 Antibody - BSA Free [NB110-40449] - Analysis of anti-SPHK1 antibody with human adrenal, pheochromocytes at concentration 10 ug/ml.")
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SPHK1 protein stained by SPHK1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20472-img0003.jpg "Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - A375 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SPHK1 protein stained by SPHK1 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP2-20472] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Sphingosine Kinase 1/SPHK1 antibody (NBP2-20472) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20472_rabbit-polyclonal-sphingosine-kinase-1-sphk1-antibody-9102024205622.jpg)
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope](http://images.novusbio.com/fullsize/Sphingosine-Kinase-1-SPHK1-Antibody-Western-Blot-NBP1-22974-img0002.jpg "Western Blot: Sphingosine Kinase 1/SPHK1 Antibody [NBP1-22974] - SPHK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti- SPHK1 antibody NBP1-22974 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SPHK1 was also immunoprecipitated by rabbit anti-SPHK1 antibody NBP1-22973, which recognizes an upstream epitope")
![Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2. Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-6B8-2F2-Western-Blot-NBP2-59338-img0004.jpg "Western Blot: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Analysis of Human, Mouse, Rat Rat Kidney Lysate showing detection of ~31 kDa HO-1 protein using Mouse Anti-HO-1 Monoclonal Antibody, Clone 6B8-2F2. Lane 1: MW Ladder. Lane 2: Rat Kidney Lysate. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-HO-1 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 5 min at RT. Predicted/Observed Size: ~31 kDa.")
![Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody (6B8-2F2) [NBP2-59338] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HO-1/HMOX1/HSP32 (Rat) Monoclonal Antibody, Clone 6B8-2F2 (NBP2-59338). Tissue: Fibroblast cell line (NIH 3T3). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HO-1/HMOX1/HSP32 (Rat) Monoclonal Antibody (NBP2-59338) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 60X.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-6B8-2F2-Immunocytochemistry-Immunofluorescence-NBP2-59338-img0005.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Western-Blot-NBP2-89374-img0002.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A: Hela Whole Cell Lysate Lane B: A549 Whole Cell Lysate Lane C: 293T Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:69 kDa Observed band size:69 kDa")
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Lane A:0.5 mg Hela Whole Cell Lysate Lane B:0.5 mg 293T Whole Cell Lysate0.5 uL anti-LTA4H rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-LTA4H rabbit monoclonal antibody,at 1:500 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions.Predicted band size: 110 kDa Observed band size: 110 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-201-Immunoprecipitation-NBP2-89374-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Leukotriene A4 Hydrolase/LTA4H Antibody (201) [NBP2-89374] - Staining of Human LTA4H in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89374 (1:300) at 4c overnight. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89374_rabbit-monoclonal-leukotriene-a4-hydrolase-lta4h-antibody-201-235202512205518.png)
![Western Blot: iNOS [p Tyr1055] Antibody [NBP3-23217] - Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1ug/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23217_rabbit-inos-p-tyr1055-pab-19920231759440.jpg "Western Blot: iNOS [p Tyr1055] Antibody [NBP3-23217] - Western blot analysis of mouse macrophages (J774A.1) treated with LPS (1ug/ml) for 18 hrs followed by pervanadate (1 mM) for 30 min. (lanes 1, 3 & 5). The blots were then treated with akaline phosphatase (lanes 2, 4 & 6). Blots were probed with rabbit polyclonal anti-inducible Nitric Oxide Synthase (iNOS) (lanes 1 & 2), anti-iNOS (Tyr-1055) (lanes 3 & 4), and mouse monoclonal anti-iNOS (lanes 5 & 6).")
![Immunocytochemistry/Immunofluorescence: iNOS [p Tyr1055] Antibody [NBP3-23217] - Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NBP3-23224), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23217_rabbit-inos-p-tyr1055-pab-19920231992812.jpg)
![Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Immunocytochemical labeling of Sphingosine Kinase 1/SPHK1 in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (NBP3-23419). The antibody was detected using goat anti-rat DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23419_rat-sphingosine-kinase-1-sphk1-mab-m540-199202312523633.jpg "Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Immunocytochemical labeling of Sphingosine Kinase 1/SPHK1 in paraformaldehyde fixed and NP-40 permeabilized A431 cells. The cells were labeled with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (NBP3-23419). The antibody was detected using goat anti-rat DyLight(R) 594.")
![Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (M540) [NBP3-23419] - Western blot image of human Hela cells (lanes 1 & 2) and recombinant his-tagged human Sphingosine Kinase 1/SPHK1 protein (lanes 3 & 4). The blots were probed with rat monoclonal anti-Sphingosine Kinase 1/SPHK1 (C-terminal Region) at 1:250 (lanes 1 & 3) and 1:1000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23419_rat-sphingosine-kinase-1-sphk1-mab-m540-19920231856961.jpg)
![Western Blot: Thioredoxin Antibody [NBP1-31090] - A: H1299 B: HeLa S3 12% SDS PAGE diluted at 1:3000](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Western-Blot-NBP1-31090-img0001.jpg "Western Blot: Thioredoxin Antibody [NBP1-31090] - A: H1299 B: HeLa S3 12% SDS PAGE diluted at 1:3000")
![Immunocytochemistry/Immunofluorescence: Thioredoxin Antibody [NBP1-31090] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Trx protein stained by Trx antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/Thioredoxin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31090-img0004.jpg)