| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-39033-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82016-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85955-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-00093SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-159SS | |||
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Analysis in human parathyroid gland and placenta tissues. Corresponding LRP2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Analysis in human parathyroid gland and placenta tissues. Corresponding LRP2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human placenta shows no positivity in trophoblastic cells as expected.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0012.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0009.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human lymph node shows no positivity in germinal center cells as expected.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0010.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human parathyroid gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0011.jpg)
![Immunohistochemistry-Paraffin: LRP2 Antibody [NBP2-39033] - Staining of human small intestine shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/LRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-39033-img0013.jpg)
![Western Blot: LRSAM1 Antibody [NBP1-82016] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-329](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Western-Blot-NBP1-82016-img0005.jpg "Western Blot: LRSAM1 Antibody [NBP1-82016] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-329")
![Immunocytochemistry/Immunofluorescence: LRSAM1 Antibody [NBP1-82016] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82016-img0006.jpg)
![Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunohistochemistry-Paraffin-NBP1-82016-img0009.jpg)
![Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunohistochemistry-Paraffin-NBP1-82016-img0001.jpg)
![Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunohistochemistry-Paraffin-NBP1-82016-img0007.jpg)
![Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human Fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunohistochemistry-Paraffin-NBP1-82016-img0008.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85955-img0010.jpg "Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human heart muscle shows strong cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85955-img0007.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85955-img0008.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody [NBP1-85955] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-Kinase-1-PDK1-Antibody-Immunohistochemistry-Paraffin-NBP1-85955-img0009.jpg)
![Western Blot: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Analysis of tyrosinase on melanoma cells lysate using anti- tyrosinase antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-C2-B5-Western-Blot-NBP2-67233-img0003.jpg "Western Blot: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Analysis of tyrosinase on melanoma cells lysate using anti- tyrosinase antibody at 1/1,000 dilution.")
![Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Staining tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-C2-B5-Immunocytochemistry-Immunofluorescence-NBP2-67233-img0004.jpg)
![Immunohistochemistry-Paraffin: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Analysis of paraffin-embedded human tyrosinase tissue using anti- tyrosinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-C2-B5-Immunohistochemistry-Paraffin-NBP2-67233-img0002.jpg)
![Flow Cytometry: Tyrosinase Antibody (C2-B5) [NBP2-67233] - Analysis of B16F1 cells with tyrosinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti mouse IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-C2-B5-Flow-Cytometry-NBP2-67233-img0001.jpg)
![Western Blot: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Western blot analysis of Tyrosinase on B16F1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Western-Blot-NBP2-67232-img0006.jpg "Western Blot: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Western blot analysis of Tyrosinase on B16F1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunocytochemistry-Immunofluorescence-NBP2-67232-img0005.jpg)
![Immunohistochemistry-Paraffin: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Analysis of paraffin-embedded human stomach cancer tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunohistochemistry-Paraffin-NBP2-67232-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunocytochemistry-Immunofluorescence-NBP2-67232-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Staining Tyrosinase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunocytochemistry-Immunofluorescence-NBP2-67232-img0002.jpg)
![Immunohistochemistry-Paraffin: Tyrosinase Antibody (JA52-11) [NBP2-67232] - Analysis of paraffin-embedded human liver tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Tyrosinase-Antibody-JA52-11-Immunohistochemistry-Paraffin-NBP2-67232-img0003.jpg)
![Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Western-Blot-NBP1-00093-img0009.jpg "Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.")
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NBP1-00093] - The SR-AI/MSR antibody was tested in Raw cells at a 1:50 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00093-img0008.jpg)
![Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.](http://images.novusbio.com/fullsize/Macrophage-Scavenger-Receptor-I-Antibody-Western-Blot-NB400-159-img0005.jpg "Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.")
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NB400-159] - Antibody was tested in Raw cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Macrophage-Scavenger-Receptor-I-Antibody-Immunocytochemistry-Immunofluorescence-NB400-159-img0006.jpg)
![Western Blot: FACL4 Antibody (GT186) [NBP3-13533] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (NBP3-13533) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/FACL4-Antibody-GT186-Western-Blot-NBP3-13533-img0004.jpg "Western Blot: FACL4 Antibody (GT186) [NBP3-13533] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (NBP3-13533) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: FACL4 Antibody (GT186) [NBP3-13533] - FACL4 antibody [GT186] detects FACL4 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. FACL4 stained by FACL4 antibody [GT186] (NBP3-13533) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/FACL4-Antibody-GT186-Immunohistochemistry-Paraffin-NBP3-13533-img0003.jpg)
![Western Blot: FACL4 Antibody (GT186) [NBP3-13533] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (NBP3-13533) diluted at 1:500.](http://images.novusbio.com/fullsize/FACL4-Antibody-GT186-Western-Blot-NBP3-13533-img0001.jpg)
![Western Blot: FACL4 Antibody (GT186) [NBP3-13533] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with FACL4 antibody [GT186] (NBP3-13533) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13533_mouse-facl4-mab-gt186-910202420122091.jpg)
![FACL4 antibody [GT186] detects FACL4 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FACL4 stained by FACL4 antibody [GT186] (NBP3-13533) diluted at 1:100.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13533_mouse-facl4-mab-gt186-immunocytochemistry-immunofluorescence-2611202518411310.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0005.jpg "Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 expression in bovine skeletal muscle whole lysate. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Glut4 Antibody [NBP1-49840] - 3.75ug/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Glut4-Antibody-Immunohistochemistry-Paraffin-NBP1-49840-img0006.jpg)
![Western Blot: Glut4 Antibody [NBP1-49840] - Glucose Transporter GLUT4 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49840 at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Glut4-Antibody-Western-Blot-NBP1-49840-img0004.jpg)
![Western Blot: SR-BI Antibody (1C1I8) [NBP3-15311] - Western blot analysis of extracts of various cell lines, using SR-BI Rabbit mAb (NBP3-15311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/SR-BI-Antibody-1C1I8-Western-Blot-NBP3-15311-img0002.jpg "Western Blot: SR-BI Antibody (1C1I8) [NBP3-15311] - Western blot analysis of extracts of various cell lines, using SR-BI Rabbit mAb (NBP3-15311) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunohistochemistry-Paraffin: SR-BI Antibody (1C1I8) [NBP3-15311] - Immunohistochemistry of paraffin-embedded rat ovary using SR-BI Rabbit mAb (NBP3-15311) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SR-BI-Antibody-1C1I8-Immunohistochemistry-Paraffin-NBP3-15311-img0001.jpg)
![Immunohistochemistry: SR-BI Antibody (1C1I8) [SR-BI] - Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SR-BI Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15311_rabbit-sr-bi-mab-1c1i8-52202517184528.jpg)
![Immunohistochemistry: SR-BI Antibody (1C1I8) [SR-BI] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using SR-BI Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15311_rabbit-sr-bi-mab-1c1i8-52202517223954.jpg)
![Immunohistochemistry: SR-BI Antibody (1C1I8) [SR-BI] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using SR-BI Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15311_rabbit-sr-bi-mab-1c1i8-5220251723571.jpg)
![Immunohistochemistry: SR-BI Antibody (1C1I8) [SR-BI] - Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using SR-BI Rabbit mAb at a dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15311_rabbit-sr-bi-mab-1c1i8-5220251726013.jpg)
![Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Western-Blot-NBP1-47398-img0011.jpg "Western Blot: NM23-H1 Antibody (4B2) [NBP1-47398] - Western blot analysis using NME1 mAb against NME1-hIgGFc transfected HEK293 cell lysate.")
![Immunocytochemistry/Immunofluorescence: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunofluorescence analysis of Hela cells using NME1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunocytochemistry-Immunofluorescence-NBP1-47398-img0010.jpg)
![Immunohistochemistry-Paraffin: NM23-H1 Antibody (4B2) [NBP1-47398] - Immunohistochemical analysis of paraffin-embedded human Placenta tissues using anti-NME1 mouse mAb.](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Immunohistochemistry-Paraffin-NBP1-47398-img0007.jpg)
![Flow Cytometry: NM23-H1 Antibody (4B2) [NBP1-47398] - Flow cytometric analysis of Jurkat cells using NME1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/NM23-H1-Antibody-4B2-Flow-Cytometry-NBP1-47398-img0009.jpg)
![Western Blot: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Western blot analysis of PLC-gamma 2 on different lysates with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 148 kDa Observed band size: 148 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32827) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-288202412461651.jpg "Western Blot: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Western blot analysis of PLC-gamma 2 on different lysates with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: Daudi cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 148 kDa Observed band size: 148 kDa Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32827) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32827) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-298202414333622.jpg)
![Immunocytochemistry/ Immunofluorescence: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Immunocytochemistry analysis of Raji cells labeling PLC-gamma 2 with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-PLC-gamma 2 antibody (NBP3-32827) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-29202410334047.jpg)
![Flow Cytometry: PLC-gamma 2 Antibody (JE39-12) [NBP3-32827] - Flow cytometric analysis of Raji cells labeling PLC-gamma 2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32827, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32827_rabbit-plc-gamma-2-mab-je39-12-392024884627.jpg)