| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44076 | |||
| BSA Free | NBP2-52899-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77252-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-37912-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62680-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87536-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48646-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58912-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12888-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-57151SS | |||
![Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Western-Blot-NBP2-52899-img0006.jpg "Western Blot: ACSL5 Antibody (CL0275) [NBP2-52899] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: ACSL5 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)")
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human small intestine shows very strong cytoplasmic and membrane immunoreactivity in the glandular cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0005.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human colon (colorectal cancer) shows moderate membrane positivity in cancer cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0001.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human colon shows strong positivity in the intestinal glands.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0002.jpg)
![Immunohistochemistry: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human duodenum shows strong cytoplasmic and membrane immunoreactivity in the glandular cells.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-NBP2-52899-img0003.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody (CL0275) [NBP2-52899] - Staining of human liver shows moderate cytoplasmic immunoreactivity in hepatocytes.](http://images.novusbio.com/fullsize/ACSL5-Antibody-CL0275-Immunohistochemistry-Paraffin-NBP2-52899-img0004.jpg)
![Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0003.jpg "Western Blot: XBP1 Antibody [NBP1-77252] - PC-3 cell lysate with XBP-1 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.")
![Western Blot: XBP1 Antibody [NBP1-77252] - Hepatic ALOX15KO inhibited alcohol-induced XBP1 up-regulation. Image from verified customer review.](http://images.novusbio.com/fullsize/XBP1-Antibody-Western-Blot-NBP1-77252-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: XBP1 Antibody [NBP1-77252] - HepG2 cells with XBP-1 antibody at 2 ug/ml.](http://images.novusbio.com/fullsize/XBP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77252-img0002.jpg)
![Western Blot: Sorbitol Dehydrogenase Antibody [NBP3-37912] - Western blot analysis of various lysates using Sorbitol Dehydrogenase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-37912_rabbit-sorbitol-dehydrogenase-pab-91220241514364.jpg "Western Blot: Sorbitol Dehydrogenase Antibody [NBP3-37912] - Western blot analysis of various lysates using Sorbitol Dehydrogenase Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Immunocytochemistry/ Immunofluorescence: Sorbitol Dehydrogenase Antibody [NBP3-37912] - Immunofluorescence analysis of NIH/3T3 cells using Sorbitol Dehydrogenase Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37912_rabbit-sorbitol-dehydrogenase-pab-912202415143695.jpg)
![Immunohistochemistry: Sorbitol Dehydrogenase Antibody [NBP3-37912] - Immunohistochemistry analysis of paraffin-embedded Human kidney using Sorbitol Dehydrogenase Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-37912_rabbit-sorbitol-dehydrogenase-pab-912202415133417.jpg)
![Immunocytochemistry/ Immunofluorescence: Sorbitol Dehydrogenase Antibody [NBP3-37912] - Immunofluorescence analysis of Huh7 cells using Sorbitol Dehydrogenase Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-37912_rabbit-sorbitol-dehydrogenase-pab-912202415133498.jpg)
![Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-62680] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Western-Blot-NBP2-62680-img0004.jpg "Western Blot: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-62680] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-62680] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunohistochemistry-Paraffin-NBP2-62680-img0007.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-62680] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunohistochemistry-Paraffin-NBP2-62680-img0002.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-62680] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunohistochemistry-Paraffin-NBP2-62680-img0005.jpg)
![Immunohistochemistry-Paraffin: Apolipoprotein E R2/ApoE R2 Antibody [NBP2-62680] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Apolipoprotein-E-R2-ApoE-R2-Antibody-Immunohistochemistry-Paraffin-NBP2-62680-img0006.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase VI Antibody [NBP1-87536] - Staining of human liver.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-87536-img0010.jpg "Immunohistochemistry-Paraffin: Carbonic Anhydrase VI Antibody [NBP1-87536] - Staining of human liver.")
![Immunohistochemistry-Paraffin: Carbonic Anhydrase VI Antibody [NBP1-87536] - Staining of human colon shows low expression as expected.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-87536-img0005.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase VI Antibody [NBP1-87536] - Staining of human salivary gland shows high expression.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-87536-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase VI Antibody [NBP1-87536] - Staining in human salivary gland and colon tissues using anti-CA6 antibody. Corresponding CA6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-87536-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Carbonic Anhydrase VI Antibody [NBP1-87536] - Staining of human colon, liver, lymph node and salivary gland using Anti-CA6 antibody NBP1-87536 (A) shows similar protein distribution across tissues to independent antibody NBP1-87537 (B).](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-87536-img0008.jpg)
![Immunohistochemistry-Paraffin: Carbonic Anhydrase VI Antibody [NBP1-87536] - Staining of human lymph node.](http://images.novusbio.com/fullsize/Carbonic-Anhydrase-VI-Antibody-Immunohistochemistry-Paraffin-NBP1-87536-img0009.jpg)
![Immunohistochemistry-Paraffin: CUGBP2 Antibody [NBP2-48646] - Immunohistochemistry analysis in human cerebral cortex and kidney tissues. Corresponding RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CUGBP2-Antibody-Immunohistochemistry-Paraffin-NBP2-48646-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CUGBP2 Antibody [NBP2-48646] - Immunohistochemistry analysis in human cerebral cortex and kidney tissues. Corresponding RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CUGBP2 Antibody [NBP2-48646] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/CUGBP2-Antibody-Immunohistochemistry-Paraffin-NBP2-48646-img0014.jpg)
![Immunohistochemistry-Paraffin: CUGBP2 Antibody [NBP2-48646] - Staining of human kidney shows no nuclear positivity in cells in tubules.](http://images.novusbio.com/fullsize/CUGBP2-Antibody-Immunohistochemistry-Paraffin-NBP2-48646-img0011.jpg)
![Immunohistochemistry-Paraffin: CUGBP2 Antibody [NBP2-48646] - Staining of human colon shows strong nuclear positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CUGBP2-Antibody-Immunohistochemistry-Paraffin-NBP2-48646-img0012.jpg)
![Immunohistochemistry-Paraffin: CUGBP2 Antibody [NBP2-48646] - Staining of human tonsil shows moderate to strong nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/CUGBP2-Antibody-Immunohistochemistry-Paraffin-NBP2-48646-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: PLC-beta 4 Antibody [NBP2-58912] - Staining of human cell line U-251 MG shows localization to microtubules. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PLC-beta-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58912-img0001.jpg "Immunocytochemistry/Immunofluorescence: PLC-beta 4 Antibody [NBP2-58912] - Staining of human cell line U-251 MG shows localization to microtubules. Antibody staining is shown in green.")
![PLC-beta 4 Antibody [NBP2-58912] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/antibody/nbp2-58912_rabbit-polyclonal-plc-beta-4-antibody-immunohistochemistry-paraffin-4520239024..jpg)
![PLC-beta 4 Antibody [NBP2-58912]- Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.](http://images.novusbio.com/fullsize/antibody/nbp2-58912_rabbit-polyclonal-plc-beta-4-antibody-immunohistochemistry-paraffin-4520239150..jpg)
![PLC-beta 4 Antibody [NBP2-58912] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/antibody/nbp2-58912_rabbit-polyclonal-plc-beta-4-antibody-immunohistochemistry-paraffin-4520239314..jpg)
![PLC-beta 4 Antibody [NBP2-58912] -Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/antibody/nbp2-58912_rabbit-polyclonal-plc-beta-4-antibody-immunohistochemistry-paraffin-4520239436..jpg)
![Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Western-Blot-NBP2-12888-img0003.jpg "Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.")
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.](http://images.novusbio.com/fullsize/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0005.jpg)
![Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-29820241420974.jpg "Immunohistochemistry: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32538) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Immunocytochemistry analysis of HeLa cells labeling LIMPII/SR-B2 with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-292024119239.jpg)
![Western Blot: LIMPII/SR-B2 Antibody (PSH03-25) [NBP3-32538] - Western blot analysis of LIMPII/SR-B2 on different lysates with Rabbit anti-LIMPII/SR-B2 antibody (NBP3-32538) at 1/5,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: HepG2 cell lysate (15 µg/Lane) Lane 3: SH-SY5Y cell lysate (15 µg/Lane) Lane 4: Neuro-2a cell lysate (15 µg/Lane) Lane 5: MEF cell lysate (15 µg/Lane) Lane 6: PC-12 cell lysate (15 µg/Lane) Lane 7: COS-1 cell lysate (15 µg/Lane) Lane 8: Mouse kidney tissue lysate (30 µg/Lane) Lane 9: Rat kidney tissue lysate (30 µg/Lane) Lane 10: Mouse liver tissue lysate (30 µg/Lane) Lane 11: Rat liver tissue lysate (30 µg/Lane) Predicted band size: 54 kDa Observed band size: 80 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32538_rabbit-limpii-sr-b2-mab-psh03-25-392024845352.jpg)
![Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-278202415212468.jpg "Western Blot: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/1,000 dilution. Lane 1: MCF7 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: Mouse brain tissue lysate (30 ug/Lane) Lane 4: U-87 MG cell lysate (30 ug/Lane) Lane 5: RAW264.7 cell lysate (30 ug/Lane) Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 ug/Lane) Lane 7: Mouse liver tissue lysate (30 ug/Lane) Lane 8: Rat liver tissue lysate (30 ug/Lane) Lane 9: Rat pancreas tissue lysate (30 ug/Lane) Predicted band size: 72 kDa Observed band size: 72 kDa Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32408) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32408) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29820241411540.jpg)
![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (C9-9-R) [NBP3-32408] - Immunocytochemistry analysis of Hela cells labeling GRP78/HSPA5 with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-GRP78/HSPA5 antibody (NBP3-32408) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32408_mouse-grp78-hspa5-mab-c9-9-r-29202410484873.png)
![Western Blot: LPIN1 Antibody [NB110-57151] - Detection of LPIN1 in 3T3L1 lysate.](http://images.novusbio.com/fullsize/LPIN1-Antibody-Western-Blot-NB110-57151-img0002.jpg "Western Blot: LPIN1 Antibody [NB110-57151] - Detection of LPIN1 in 3T3L1 lysate.")
