| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-26393SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89293-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89292-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80670-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-00093SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-159SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70528 | |||
![Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2682024158262.jpg "Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Huh7 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32722) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-27820241631640.jpg)
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29820241492649.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Carboxylase with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2920241023429.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32722, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29202416372196.jpg)
![Western Blot: SCD Antibody [NBP1-26393] - Normal human liver.](http://images.novusbio.com/fullsize/SCD-Antibody-Western-Blot-NBP1-26393-img0001.jpg "Western Blot: SCD Antibody [NBP1-26393] - Normal human liver.")
![Immunocytochemistry/Immunofluorescence: SCD Antibody [NBP1-26393] - SCD staining in Hela cells detected with a Dylight 488 labeled secondary antibody (Green) with Hoechst 33258 nuclear counterstain (Blue).](http://images.novusbio.com/fullsize/SCD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-26393-img0004.jpg)
![Immunohistochemistry: ACADM Antibody [NBP1-89293] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-NBP1-89293-img0013.jpg "Immunohistochemistry: ACADM Antibody [NBP1-89293] - Staining of human skin shows no positivity in squamous epithelial cells as expected.")
![Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89293] - Staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89293-img0002.jpg)
![Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89293] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89293-img0006.jpg)
![Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89293] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89293-img0007.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89293] - Staining in human kidney and skin tissues using anti-ACADM antibody. Corresponding ACADM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89293-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89293] - Staining of human kidney, liver, lymph node and skin using Anti-ACADM antibody NBP1-89293 (A) shows similar protein distribution across tissues to independent antibody NBP1-89292 (B).](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89293-img0010.jpg)
![Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89293] - Staining of human liver shows strong to very strong positivity in cytoplasm granular in hepatocytes.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89293-img0011.jpg)
![Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89293] - Staining of human lymph node shows strong granular cytoplasmic positivity in germinal center cells.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89293-img0012.jpg)
![Western Blot: ACADM Antibody [NBP1-89292] - Staining of human kidney shows high expression.](http://images.novusbio.com/fullsize/ACADM-Antibody-Western-Blot-NBP1-89292-img0008.jpg "Western Blot: ACADM Antibody [NBP1-89292] - Staining of human kidney shows high expression.")
![Immunocytochemistry/Immunofluorescence: ACADM Antibody [NBP1-89292] - Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89292-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human kidney, liver, lymph node and skin using Anti-ACADM antibody NBP1-89292 (A) shows similar protein distribution across tissues to independent antibody NBP1-89293 (B).](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89292-img0011.jpg)
![Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction center.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89292-img0002.jpg)
![Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human skin shows low expression as expected.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89292-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining in human kidney and skin tissues using anti-ACADM antibody. Corresponding ACADM RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89292-img0010.jpg)
![Immunohistochemistry-Paraffin: ACADM Antibody [NBP1-89292] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/ACADM-Antibody-Immunohistochemistry-Paraffin-NBP1-89292-img0012.jpg)
![Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining in human thyroid gland and cerebral cortex tissues using anti-TPO antibody. Corresponding TPO RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Thyroid-Peroxidase-Antibody-Immunohistochemistry-Paraffin-NBP1-80670-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining in human thyroid gland and cerebral cortex tissues using anti-TPO antibody. Corresponding TPO RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Thyroid-Peroxidase-Antibody-Immunohistochemistry-Paraffin-NBP1-80670-img0002.jpg)
![Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-80670] - Staining of human thyroid gland shows high expression.](http://images.novusbio.com/fullsize/Thyroid-Peroxidase-Antibody-Immunohistochemistry-Paraffin-NBP1-80670-img0003.jpg)
![Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Western-Blot-NBP1-00093-img0009.jpg "Western Blot: SR-AI/MSR Antibody [NBP1-00093] - Detection of SR-AI/MSR in human liver.")
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NBP1-00093] - The SR-AI/MSR antibody was tested in Raw cells at a 1:50 dilution against DyLight 488 (Green). Alpha-tubulin and nuclei were counterstained against DyLight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SR-AI-MSR-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-00093-img0008.jpg)
![Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.](http://images.novusbio.com/fullsize/Macrophage-Scavenger-Receptor-I-Antibody-Western-Blot-NB400-159-img0005.jpg "Western Blot: SR-AI/MSR Antibody [NB400-159] - Detection of MSR in human liver using NB400-159.")
![Immunocytochemistry/Immunofluorescence: SR-AI/MSR Antibody [NB400-159] - Antibody was tested in Raw cells at a 1:50 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/Macrophage-Scavenger-Receptor-I-Antibody-Immunocytochemistry-Immunofluorescence-NB400-159-img0006.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Western-Blot-H00023569-M01-img0001.jpg "Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (4D8) [H00023569-M01] - Analysis of monoclonal antibody to PADI4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Immunocytochemistry-Immunofluorescence-H00023569-M01-img0004.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Detection of enzymatically active peptidyl arginine deiminase (PAD) in the synovial fluid (SF) of patients with rheumatoid arthritis (RA). A, Immunoblotting of albumin‐depleted SF samples from 8 RA patients shows presence of neutrophil elastase (NE), PAD4, & PAD2. B, PAD activity in the SF of patients with RA (n = 12) was significantly higher than that in the SF of patients with osteoarthritis (OA) (n = 9). C, PAD activity was observed in the OA SF & RA SF both at physiologic calcium concentrations & at higher calcium concentrations (5 mM). In B & C, symbols represent individual patients; horizontal bars show the median. D, PAD activity is significantly correlated with DNA levels in the SF of RA patients (n = 22). Data were analyzed using Spearman's rho test for correlation. ∗ = P < 0.05; ∗∗ = P < 0.01, by Mann‐Whitney U test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415482220.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Release of peptidyl arginine deiminases (PADs) into the supernatant during NETosis, & detection of enzymatically active PADs. A, DNA release in peripheral blood neutrophils from healthy donors that were left unstimulated or stimulated with 25 mM phorbol myristate acetate (PMA) was assessed for up to 240 minutes after stimulation. Bars show the mean ± SD of 8 samples per group. B, For isolation of neutrophil extracellular traps, supernatants (SN) of unstimulated (unst.) or stimulated (stim.) neutrophils were collected. Subsequently, the cells were washed 3 times with RPMI medium (W1–W3), & stimulated cells were incubated in the absence or presence of DNase I; unstimulated cells treated with DNase I served as a control. Bars show the mean ± SD DNA concentration (conc) in 7 samples per group. C, Proteins (PAD2, PAD4, & neutrophil elastase [NE]) were precipitated from the same supernatants of unstimulated or stimulated cells as described in B & analyzed by Western blotting. Citrullinated proteins (citr.prot.) were detected using chemical modification & anti–modified citrulline antibody. PAD2 & PAD4 antibodies were tested for cross‐reactivity using human (hu) recombinant PAD4 (rPAD4) (250 ng) & human skeletal muscle tissue lysate (15 μg). One representative blot of 4 independent experiments is shown for each group. D, PAD activity in supernatants of unstimulated & stimulated cells was compared. Symbols represent individual donors (n = 10); bars show the median. ∗ = P < 0.05; ∗∗ = P < 0.01, by Wilcoxon's matched pairs signed rank test. AFU = arbitrary fluorescence units; NS = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415485135.jpg)
![Western Blot: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2C9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2C9.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C9-Antibody-1D7-Western-Blot-NBP2-01397-img0002.jpg "Western Blot: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2C9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 2C9.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2C9.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C9-Antibody-1D7-Immunocytochemistry-Immunofluorescence-NBP2-01397-img0005.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - Staining of paraffin-embedded Human liver tissue using anti-Cytochrome P450 2C9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C9-Antibody-1D7-Immunohistochemistry-Paraffin-NBP2-01397-img0004.jpg)
![Flow Cytometry: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 2C9 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C9-Antibody-1D7-Flow-Cytometry-NBP2-01397-img0001.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C9 Antibody (1D7) [NBP2-01397] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Cytochrome P450 2C9 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C9-Antibody-1D7-Immunohistochemistry-Paraffin-NBP2-01397-img0003.jpg)
![Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357] - Western blot analysis of extracts of various cell lines, using Catalase Rabbit mAb (NBP3-15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/Catalase-Antibody-7D2Y4-Western-Blot-NBP3-15357-img0002.jpg "Western Blot: Catalase Antibody (7D2Y4) [NBP3-15357] - Western blot analysis of extracts of various cell lines, using Catalase Rabbit mAb (NBP3-15357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: Catalase Antibody (7D2Y4) [NBP3-15357] - Immunohistochemistry of paraffin-embedded Mouse kidney using Catalase antibody (NBP3-15357) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Catalase-Antibody-7D2Y4-Immunohistochemistry-Paraffin-NBP3-15357-img0001.jpg)
![Western Blot: Catalase Antibody (7D2Y4) [Catalase] - Western blot analysis of lysates from Hep G2 cells using Catalase Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517213587.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-5220251728490.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517223975.jpg)
![Immunohistochemistry: Catalase Antibody (7D2Y4) [Catalase] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Catalase Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15357_rabbit-catalase-mab-7d2y4-52202517172853.jpg)
![Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Western blot analysis using RBP4 mAb against HEK293 (1) and RBP4(AA: 1-201)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-Western-Blot-NBP2-37614-img0002.jpg "Western Blot: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Western blot analysis using RBP4 mAb against HEK293 (1) and RBP4(AA: 1-201)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Immunofluorescence analysis of HepG2 cells using RBP4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-Immunocytochemistry-Immunofluorescence-NBP2-37614-img0003.jpg)
![Immunohistochemistry: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and stomach cancer tissues (right) using RBP4 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-Immunohistochemistry-NBP2-37614-img0005.jpg)
![Flow Cytometry: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Flow cytometric analysis of HepG2 cells using RBP4 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-Flow-Cytometry-NBP2-37614-img0004.jpg)
![ELISA: RBP4/Retinol-Binding Protein 4 Antibody (4C2) [NBP2-37614] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/RBP4-Retinol-Binding-Protein-4-Antibody-4C2-ELISA-NBP2-37614-img0001.jpg)
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-12 cell lysate Lane 4: mouse lymph node tissue Lane 5: rat liver tissue Lane 6: rat brain tissue Lysates/proteins at 30 ug/Lane. Predicted band size: 129.6 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32723) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-278202415295530.jpg "Western Blot: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HeLa cell lysate Lane 3: PC-12 cell lysate Lane 4: mouse lymph node tissue Lane 5: rat liver tissue Lane 6: rat brain tissue Lysates/proteins at 30 ug/Lane. Predicted band size: 129.6 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32723) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32723) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-29820241420961.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Immunocytochemistry analysis of PC-12 cells labeling PBC with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32723) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-29202410334022.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-56) [NBP3-32723] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32723, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32723_rabbit-pyruvate-carboxylase-mab-psh01-56-392024884671.jpg)