| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-11914-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90272-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99029-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89345-50ul | |||
![Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18 P3 B6) [NBP3-30887] - A. and B. Western blot using 3A5 antibody (Cat. No. NBP3-30887) on recombinant CYP450 proteins.C. Immunohistochemistry stain using 3A5 antibody on oesophagus cancer tissue.](http://images.novusbio.com/fullsize/nbp3-30887_mouse-cytochrome-p450-3a5-mab-f18-p3-b6-811202420592140.jpg "Immunohistochemistry: Cytochrome P450 3A5 Antibody (F18 P3 B6) [NBP3-30887] - A. and B. Western blot using 3A5 antibody (Cat. No. NBP3-30887) on recombinant CYP450 proteins.C. Immunohistochemistry stain using 3A5 antibody on oesophagus cancer tissue.")
![Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483] - Western blot analysis of IRP2 on different lysates with Mouse anti-IRP2 antibody (NBP3-32483) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Mouse liver tissue lysate (40 ug/Lane) Lane 4: Mouse kidney tissue lysate (40 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32483) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32483_mouse-irp2-mab-9d1-r-27820241631635.jpg "Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483] - Western blot analysis of IRP2 on different lysates with Mouse anti-IRP2 antibody (NBP3-32483) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Mouse liver tissue lysate (40 ug/Lane) Lane 4: Mouse kidney tissue lysate (40 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32483) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.")
![Western Blot: PDE8A Antibody (EM-52) [NBP2-62213] - Analysis (reducing conditions) of PDE8a in HEK293T/17-PDE8a transfectants using mouse monoclonal antibody EM-52.](http://images.novusbio.com/fullsize/PDE8A-Antibody-EM-52-Western-Blot-NBP2-62213-img0001.jpg "Western Blot: PDE8A Antibody (EM-52) [NBP2-62213] - Analysis (reducing conditions) of PDE8a in HEK293T/17-PDE8a transfectants using mouse monoclonal antibody EM-52.")
![Western Blot: CYP3A7 Antibody (F19 P2 H2) [NBP3-11914] - A. Western blot analysis using CYP3A7 antibody on various recombinant CYP450 proteins. B. Immunohistochemistry staining of prostate cancer tissue using CYP3A7 antibody.](http://images.novusbio.com/fullsize/CYP3A7-Antibody-F19-P2-H2-Western-Blot-NBP3-11914-img0001.jpg "Western Blot: CYP3A7 Antibody (F19 P2 H2) [NBP3-11914] - A. Western blot analysis using CYP3A7 antibody on various recombinant CYP450 proteins. B. Immunohistochemistry staining of prostate cancer tissue using CYP3A7 antibody.")
![Western Blot: Aspartate Aminotransferase Antibody (A2D6-R) [NBP3-32050] - Western blot analysis of Aspartate Aminotransferase on different lysates with Mouse anti-Aspartate Aminotransferase antibody (NBP3-32050) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Mouse liver tissue lysate (40 ug/Lane) Lane 7: Mouse heart tissue lysate (40 ug/Lane) Lane 8: Rat liver tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 46 kDa Observed band size: 38 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32050_mouse-aspartate-aminotransferase-mab-a2d6-r-278202416331228.jpg "Western Blot: Aspartate Aminotransferase Antibody (A2D6-R) [NBP3-32050] - Western blot analysis of Aspartate Aminotransferase on different lysates with Mouse anti-Aspartate Aminotransferase antibody (NBP3-32050) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: K-562 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: Caco-2 cell lysate (20 ug/Lane) Lane 5: Human liver tissue lysate (40 ug/Lane) Lane 6: Mouse liver tissue lysate (40 ug/Lane) Lane 7: Mouse heart tissue lysate (40 ug/Lane) Lane 8: Rat liver tissue lysate (40 ug/Lane) Lane 9: Rat heart tissue lysate (40 ug/Lane) Lysates/proteins at 10 ug/Lane. Predicted band size: 46 kDa Observed band size: 38 kDa Exposure time: 1 minute 41 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: CYP4F2 Antibody [NBP2-87240] - WB Suggested Anti-CYP4F2 Antibody. Titration: 1.0 ug/ml. Positive Control: HepG2 Whole CellCYP4F2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells](http://images.novusbio.com/fullsize/CYP4F2-Antibody-Western-Blot-NBP2-87240-img0001.jpg "Western Blot: CYP4F2 Antibody [NBP2-87240] - WB Suggested Anti-CYP4F2 Antibody. Titration: 1.0 ug/ml. Positive Control: HepG2 Whole CellCYP4F2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells")
![Western Blot: CYP4F2 Antibody [NBP2-87240] - Host: Rabbit. Target: CYP4F2. Positive control (+): Human Liver (LI). Negative control (-): Human Brain (BR). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/CYP4F2-Antibody-Western-Blot-NBP2-87240-img0002.jpg)
![Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Western-Blot-NBP2-90272-img0001.jpg "Western Blot: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A: THP-1 Whole Cell LysateLane B: HL-60 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:17 kDaObserved band size:15 kDa")
![Immunoprecipitation: AIF-1/Iba1 Antibody (145) [NBP2-90272] - Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg THP-1 Whole Cell Lysate2 uL anti-AIF1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AIF1 rabbit monoclonal antibody,at 1:100 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 17 kDaObserved band size: 17 kDa](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-145-Immunoprecipitation-NBP2-90272-img0002.jpg)
![Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Western-Blot-NBP2-99029-img0002.jpg "Western Blot: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody at 1:500 dilution. Lane A: A549 Whole Cell Lysate. Lysates/proteins at 60 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 70 kDa. Observed band size: 63 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Leukotriene A4 Hydrolase/LTA4H Antibody [NBP2-99029] - Leukotriene A4 Hydrolase/LTA4H was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate. 2 ul anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Leukotriene A4 Hydrolase/LTA4H rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 69 kDa. Observed band size: 69 kDa](http://images.novusbio.com/fullsize/Leukotriene-A4-Hydrolase-LTA4H-Antibody-Immunoprecipitation-NBP2-99029-img0001.jpg)
![Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Leptin-OB-Antibody-002-Flow-Cytometry-NBP2-89345-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Leptin/OB Antibody (002) [NBP2-89345] - Analysis of Human LEP expression on HeLa cells. The cells were treated, stained with purified anti-Human LEP, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Leptin/OB Antibody (002) [NBP2-89345] - Staining of LEP in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP2-89345 (dilution ratio 1:60) at 4c overnight. Then cells were stained with the Alexa Fluor(R)594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to Cytoplasm .](http://images.novusbio.com/fullsize/nbp2-89345_rabbit-monoclonal-leptin-ob-antibody-002-23520251129371.png)
![Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32409) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 72 kDa Observed band size: 70/72 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32409) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32409_mouse-grp78-hspa5-mab-a5-c9-g6-r-278202415242693.jpg "Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32409) at 1/2,000 dilution. Lane 1: HeLa cell lysate (20 ug/Lane) Lane 2: HepG2 cell lysate (20 ug/Lane) Lane 3: NIH/3T3 cell lysate (20 ug/Lane) Lane 4: MCF7 cell lysate (20 ug/Lane) Lane 5: Mouse testis tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 72 kDa Observed band size: 70/72 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32409) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402] - Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (NBP3-32402) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: U87-MG cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 81 kDa Observed band size: 68 kDa Exposure time: 10s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32402_rabbit-gpd2-mab-psh0-31-278202416331284.png "Western Blot: GPD2 Antibody (PSH0-31) [NBP3-32402] - Western blot analysis of GPD2 on different lysates with Rabbit anti-GPD2 antibody (NBP3-32402) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate Lane 2: THP-1 cell lysate Lane 3: U87-MG cell lysate Lane 4: HeLa cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 81 kDa Observed band size: 68 kDa Exposure time: 10s; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32402) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Flow Cytometry: GPD2 Antibody (PSH0-31) [NBP3-32402] - Flow cytometric analysis of Hela cells labeling GPD2. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32402, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32402_rabbit-gpd2-mab-psh0-31-392024884666.png)