| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73364 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35630-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03522-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92202-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-97524-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89830-50ul | |||
![Western Blot: PGM3 Antibody (OTI5C6) [NBP2-46380] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3.](http://images.novusbio.com/fullsize/PGM3-Antibody-OTI5C6-Western-Blot-NBP2-46380-img0003.jpg "Western Blot: PGM3 Antibody (OTI5C6) [NBP2-46380] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGM3.")
![Immunohistochemistry: PGM3 Antibody (OTI5C6) [NBP2-46380] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/PGM3-Antibody-OTI5C6-Immunohistochemistry-NBP2-46380-img0002.jpg)
![Flow Cytometry: PGM3 Antibody (OTI5C6) [NBP2-46380] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by PGM3 antibody.](http://images.novusbio.com/fullsize/PGM3-Antibody-OTI5C6-Flow-Cytometry-NBP2-46380-img0001.jpg)
![Western Blot: MCT2 Antibody [NB300-877] - (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MCT2-Antibody-Western-Blot-NB300-877-img0002.jpg "Western Blot: MCT2 Antibody [NB300-877] - (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: MCT2 Antibody [NB300-877] - Staining of Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/MCT2-Antibody-Immunohistochemistry-Paraffin-NB300-877-img0003.jpg)
![Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human ACAT2 protein](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-Western-Blot-NBP2-42614-img0001.jpg "Western Blot: ACAT2 Antibody (55D5) [NBP2-42614] - The recombinant protein (50ng) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human ACAT2 protein")
![Immunocytochemistry/Immunofluorescence: ACAT2 Antibody (55D5) [NBP2-42614] - ICC/IF analysis of ACAT2 in HeLa cells. The cell was stained with ATGA0335 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-Immunocytochemistry-Immunofluorescence-NBP2-42614-img0004.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - The cell lysates(5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: ACAT2 Transfected 293T cell lysate](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0002.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACAT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HepG2 cell lysateLane 2.: Ramos cell lysateLane 3.: MCF7 cell lysateLane 4.: HL-60 cell lysateLane 5.: SW480 cell lysate](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0003.jpg)
![ACAT2 Antibody (55D5) [NBP2-42614] - Analysis of ACAT2 in PC3 cells. The cells were stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mousemonoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/ACAT2-Antibody-55D5-NBP2-42614-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP3-35630] - Immunofluorescence analysis of C6 cells using ETFB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35630_rabbit-etfb-pab-912202415161829.jpg "Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP3-35630] - Immunofluorescence analysis of C6 cells using ETFB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP3-35630] - Immunofluorescence analysis of U-2 OS cells using ETFB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35630_rabbit-etfb-pab-912202414112844.jpg)
![Immunocytochemistry/ Immunofluorescence: ETFB Antibody [NBP3-35630] - Immunofluorescence analysis of L929 cells using ETFB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35630_rabbit-etfb-pab-912202415121391.jpg)
![Western Blot: ETFB Antibody [NBP3-35630] - Western blot analysis of various lysates using ETFB Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.](http://images.novusbio.com/fullsize/nbp3-35630_rabbit-etfb-pab-912202414213765.jpg)
![Western Blot: PI 3-Kinase p85 alpha Antibody (7Z9H0) [NBP3-16525] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 alpha Rabbit mAb (NBP3-16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/PI-3-Kinase-p85-alpha-Antibody-7Z9H0-Western-Blot-NBP3-16525-img0002.jpg "Western Blot: PI 3-Kinase p85 alpha Antibody (7Z9H0) [NBP3-16525] - Western blot analysis of extracts of various cell lines, using PI 3-Kinase p85 alpha Rabbit mAb (NBP3-16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.")
![Western Blot: PI 3-Kinase p85 alpha Antibody (3X3T8) [NBP3-16525] - Western blot analysis of various lysates, using PI 3-Kinase p85 alpha Rabbit mAb at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp3-16525_rabbit-pi-3-kinase-p85-alpha-mab-3x3t8-52202511365130.jpg)
![Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.](http://images.novusbio.com/fullsize/GAPDH-2-Antibody-Western-Blot-NBP3-03522-img0001.jpg "Western Blot: GAPDH-2 Antibody [NBP3-03522] - Analysis of extracts of various cell lines, using GAPDH-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.")
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Rat heart using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516334192.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded human breast using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-52202516293948.jpg)
![Immunohistochemistry: GAPDH-2 Antibody - BSA Free [GAPDH-2] - Immunohistochemistry analysis of paraffin-embedded Mouse testis using GAPDH-2 Rabbit pAb at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol](http://images.novusbio.com/fullsize/nbp3-03522_rabbit-polyclonal-gapdh-2-antibody-5220251628067.jpg)
![Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Western-Blot-NBP2-92202-img0002.jpg "Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ACSS1 Antibody [NBP2-92202] - Analysis of L929 cells using ACSS1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92202-img0001.jpg)
![Immunohistochemistry-Paraffin: ACSS1 Antibody [NBP2-92202] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACSS1 Rabbit pAb (NBP2-92202) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-92202-img0003.jpg)
![Western Blot: ETFB Antibody [NBP2-97524] - Anti-ETFB rabbit polyclonal antibody at 1:500 dilution. Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa](http://images.novusbio.com/fullsize/ETFB-Antibody-Western-Blot-NBP2-97524-img0005.jpg "Western Blot: ETFB Antibody [NBP2-97524] - Anti-ETFB rabbit polyclonal antibody at 1:500 dilution. Lane A: MOLT4 Whole Cell Lysate Lane B: Mouse liver tissue lysate Lane C: U-251 MG Whole Cell Lysate Lane D: NIH-3T3 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 27 kDa. Observed band size: 27 kDa")
![Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524] - Immunochemical staining of human ETFB in human liver with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ETFB-Antibody-Immunohistochemistry-Paraffin-NBP2-97524-img0004.jpg)
![Immunohistochemistry-Paraffin: ETFB Antibody [NBP2-97524] - Immunochemical staining of human ETFB in cynomolgus heart with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/ETFB-Antibody-Immunohistochemistry-Paraffin-NBP2-97524-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ETFB Antibody [NBP2-97524] - Immunofluorescence staining of ETFB in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-97524 antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp2-97524_rabbit-polyclonal-etfb-antibody-235202514492380.png)
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-288202412442923.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Western blot analysis of Pyruvate Dehydrogenase E1-alpha subunit on different lysates with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: Jurkat cell lysate (20 ug/Lane) Lane 3: HepG2 cell lysate (20 ug/Lane) Lane 4: HeLa cell lysate (20 ug/Lane) Lane 5: HEK-293 cell lysate (20 ug/Lane) Lane 6: Human kidney tissue lysate (40 ug/Lane) Lane 7: Mouse brain tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Predicted band size: 43 kDa Observed band size: 43 kDa Exposure time: 39 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32787) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32787) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-298202414115418.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody (JE58-27) [NBP3-32787] - Immunocytochemistry analysis of Jurkat cells treated with or without lambda pp labeling Pyruvate Dehydrogenase E1-alpha subunit with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Dehydrogenase E1-alpha subunit antibody (NBP3-32787) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32787_rabbit-pyruvate-dehydrogenase-e1-alpha-subunit-p-ser293-mab-2920241114815.jpg)
![Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-278202415295516.jpg "Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-HADH antibody (NBP3-32416) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32416) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29820241420931.jpg)
![Immunocytochemistry/ Immunofluorescence: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunocytochemistry analysis of HeLa cells labeling HADH with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29202410334032.jpg)
![Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-064-Western-Blot-NBP2-89830-img0001.jpg "Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa")
![Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human liver with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-235202511545677.png)
![Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human kidney with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-235202511444489.png)
![Immunoprecipitation: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - ENTPD5 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL NBP2-89830 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP2-89830 at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 47 kDa](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-23520251154565.png)
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)