| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71790 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73228 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73332 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92924-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37271-0.025ml | |||
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024936274.jpg "Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422681.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425469.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249425455.jpg)
![Immunohistochemistry-Paraffin: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - IGF1R antibody [HL1957] detects IGF1R protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IGF1R stained by IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-2120249422643.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: IGF-I R/IGF1R Antibody (HL1957) - Azide and BSA Free [NBP3-25519] - Wild-type (WT) and IGF1R knockout (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with IGF1R antibody [HL1957] (NBP3-25519) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25519_rabbit-igf-i-r-igf1r-mab-hl1957-azide-and-bsa-free-212024940886.jpg)
![Western Blot: ARH Antibody (7E11) [NBP2-01435] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARH.](http://images.novusbio.com/fullsize/ARH-Antibody-7E11-Western-Blot-NBP2-01435-img0010.jpg "Western Blot: ARH Antibody (7E11) [NBP2-01435] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARH (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARH.")
![Immunocytochemistry/Immunofluorescence: ARH Antibody (OTI7E11) [NBP2-01435] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ARH.](http://images.novusbio.com/fullsize/ARH-Antibody-7E11-Immunocytochemistry-Immunofluorescence-NBP2-01435-img0007.jpg)
![Immunohistochemistry-Paraffin: ARH Antibody (7E11) [NBP2-01435] - Staining of paraffin-embedded Human Kidney tissue using anti-ARH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ARH-Antibody-7E11-Immunohistochemistry-Paraffin-NBP2-01435-img0009.jpg)
![Flow Cytometry: ARH Antibody (7E11) [NBP2-01435] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARH antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/ARH-Antibody-7E11-Flow-Cytometry-NBP2-01435-img0006.jpg)
![Immunohistochemistry-Paraffin: ARH Antibody (7E11) [NBP2-01435] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-ARH mouse monoclonal antibody.](http://images.novusbio.com/fullsize/ARH-Antibody-7E11-Immunohistochemistry-Paraffin-NBP2-01435-img0008.jpg)
![Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Western-Blot-NBP2-46310-img0008.jpg "Western Blot: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PADI4.")
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0007.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0001.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0002.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0003.jpg)
![Immunohistochemistry: PADI4 Antibody (OTI5C10) [NBP2-46310] - Human Ovary tissue within the normal limits using anti-PADI4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0004.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0005.jpg)
![Immunohistochemistry-Paraffin: PADI4 Antibody (OTI5C10) [NBP2-46310] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/PADI4-Antibody-5C10-Immunohistochemistry-NBP2-46310-img0006.jpg)
![Western Blot: PDX-1/IPF1 Antibody (OTI3H3) [NBP2-46371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 .](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Western-Blot-NBP2-46371-img0016.jpg "Western Blot: PDX-1/IPF1 Antibody (OTI3H3) [NBP2-46371] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 .")
![Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (OTI3H3) [NBP2-46371] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-46371-img0013.jpg)
![Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (OTI3H3) [NBP2-46371] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-46371-img0014.jpg)
![Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (OTI3H3) [NBP2-46371] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-46371-img0015.jpg)
![Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (OTI3H3) [NBP2-46371] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PDX1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-46371-img0005.jpg)
![Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (OTI3H3) [NBP2-46371] - Staining paraffin-embedded Human liver tissue using anti-PDX1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-46371-img0009.jpg)
![Immunohistochemistry-Paraffin: PDX-1/IPF1 Antibody (OTI3H3) [NBP2-46371] - Staining of paraffin-embedded Human Kidney tissue using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,2) (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Immunohistochemistry-Paraffin-NBP2-46371-img0011.jpg)
![Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Western-Blot-NBP1-97762-img0004.jpg "Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A1 Antibody [NBP1-97762] - Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-97762-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-97762-img0006.jpg)
![Western Blot: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of Rat Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.1 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Western-Blot-NBP1-97762-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 1A1 Antibody [NBP1-97762] - Staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with Tris-EDTA pH 9, HRP-Staining. Antibody at 1:500.](http://images.novusbio.com/fullsize/Cytochrome-P450-1A1-Antibody-Immunohistochemistry-Paraffin-NBP1-97762-img0005.jpg)
![Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Western-Blot-NB100-1448-img0007.jpg "Western Blot: 58K Golgi Protein Antibody [NB100-1448] - Staining of liver lysates (A) Human with antibody at 0.1 ug/mL, Mouse (B) and Pig (C) with antibody at 0.03 ug/mL (35 ug protein in RIPA buffer). Detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1448-img0009.jpg)
![Flow Cytometry: 58K Golgi Protein Antibody [NB100-1448] - Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Flow-Cytometry-NB100-1448-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: 58K Golgi Protein Antibody [NB100-1448] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/58K-Golgi-Protein-Antibody-Immunocytochemistry-Immunofluorescence-NB100-1448-img0008.jpg)
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0003.jpg "Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.")
![Western Blot: RXR alpha/NR2B1 Antibody (6Y10M1) [NBP3-15667] - Western blot analysis of extracts of various cell lines, using RXR alpha/NR2B1 antibody (NBP3-15667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-6Y10M1-Western-Blot-NBP3-15667-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Confocal imaging of HeLa cells using RXR alpha/NR2B1 Rabbit mAb . The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-5220251731103.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR alpha/NR2B1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of RXR alpha/NR2B1 across chromosome 19 (upper panel) and the genomic region encompassing ECH1, a representative gene enriched in RXR alpha/NR2B1 (lower panel).](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517344269.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation was performed with 25 ug of cross-linked chromatin from HepG2 cells using 5 ug of RXR alpha/NR2B1 Rabbit mAb . DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 . The ChIP sequencing results indicate the enrichment pattern of RXR alpha/NR2B1 in the representative genomic region surrounding ECH1 gene.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517315534.jpg)
![Chromatin Immunoprecipitation: RXR alpha/NR2B1 Antibody (6Y10M1) [RXR alpha/NR2B1] - Chromatin immunoprecipitation analysis of extracts from HepG2 cells, using RXR alpha/NR2B1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/nbp3-15667_rabbit-rxr-alpha-nr2b1-mab-6y10m1-52202517352787.jpg)
![Western Blot: CAD Antibody [NBP2-92924] - Analysis of extracts of various cell lines, using CAD at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/CAD-Antibody-Western-Blot-NBP2-92924-img0009.jpg "Western Blot: CAD Antibody [NBP2-92924] - Analysis of extracts of various cell lines, using CAD at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Rat testis using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0008.jpg)
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Human tonsil using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0006.jpg)
![Immunohistochemistry-Paraffin: CAD Antibody [NBP2-92924] - Mouse spleen using CAD antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/CAD-Antibody-Immunohistochemistry-Paraffin-NBP2-92924-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CAD Antibody [NBP2-92924] - Analysis of NIH-3T3 cells using CAD at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CAD-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92924-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: CAD Antibody - BSA Free [NBP2-92924] - Immunofluorescence analysis of U-2 OS cells using CAD Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92924_rabbit-polyclonal-cad-antibody-4220251429027.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0004.jpg "Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mouse mAb against A431 cell lysate.")
![Immunohistochemistry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of esophageal cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0007.jpg)
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of A431 cells using NT5E mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Flow-Cytometry-NBP2-37271-img0005.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against human NT5E (AA: 30-250) recombinant protein. (Expected MW is 26.6 kDa)](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0002.jpg)
![Western Blot: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis using NT5E mAb against HEK293 (1) and NT5E (AA: 30-250)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Western-Blot-NBP2-37271-img0003.jpg)
![Immunohistochemistry-Paraffin: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Analysis of endometrial cancer tissues using NT5E mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-Immunohistochemistry-NBP2-37271-img0006.jpg)
![ELISA: 5'-Nucleotidase/CD73 Antibody (4G6E3) [NBP2-37271] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/5-Nucleotidase-CD73-Antibody-4G6E3-ELISA-NBP2-37271-img0001.jpg)
![Western Blot: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 .](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Western-Blot-NBP2-73332-img0001.jpg "Western Blot: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDX1 .")
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining of paraffin-embedded Human Kidney tissue using anti-PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min,2) (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0006.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0002.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0003.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti PDX1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0004.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-PDX1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0005.jpg)
![Immunohistochemistry: PDX-1/IPF1 Antibody (OTI3H3) - Azide and BSA Free [NBP2-73332] - Staining paraffin-embedded Human liver tissue using anti-PDX1 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150)](http://images.novusbio.com/fullsize/PDX-1-IPF1-Antibody-OTI3H3-Azide-and-BSA-Free-Immunohistochemistry-NBP2-73332-img0007.jpg)
![Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2682024158262.jpg "Immunoprecipitation: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Pyruvate Carboxylase was immunoprecipitated in 0.2mg HeLa cell lysate with NBP3-32722 at 2 ug/25 ul agarose. Western blot was performed from the immunoprecipitate using NBP3-32722 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: Rabbit IgG instead of NBP3-32722 in HeLa cell lysate Lane 3: NBP3-32722 IP in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 24 seconds")
![Western Blot: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Western blot analysis of Pyruvate Carboxylase on different lysates with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: MCF7 cell lysate Lane 3: A549 cell lysate Lane 4: Huh7 cell lysate Lane 5: Hela cell lysate Lane 6: NIH/3T3 cell lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 130 kDa Observed band size: 130 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32722) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-27820241631640.jpg)
![Immunohistochemistry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32722) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29820241492649.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Immunocytochemistry analysis of HepG2 cells labeling Pyruvate Carboxylase with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Pyruvate Carboxylase antibody (NBP3-32722) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-2920241023429.jpg)
![Flow Cytometry: Pyruvate Carboxylase Antibody (PSH01-55) [NBP3-32722] - Flow cytometric analysis of PC-12 cells labeling Pyruvate Carboxylase. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32722, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32722_rabbit-pyruvate-carboxylase-mab-psh01-55-29202416372196.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ECH1 Antibody [NBP2-16261] - Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody diluted at 1:2000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: ECH1 Antibody [NBP2-16261] - Confocal immunofluorescence analysis of methanol-fixed HeLa, using ECH1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16261-img0003.jpg)
![Immunohistochemistry-Paraffin: ECH1 Antibody [NBP2-16261] - ECH1 antibody detects ECH1 protein at mitochondrion on Cal27 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded Cal27 xenograft. ECH1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunohistochemistry-Paraffin-NBP2-16261-img0004.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0001.jpg)
![Western Blot: ECH1 Antibody [NBP2-16261] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 10% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP2-16261-img0002.jpg)