| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88054-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-14276-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89534-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82016-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87203-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-92275-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48148 | |||
| Azide and BSA Free | NBP3-11456 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77459SS | |||
![Western Blot: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis using Anti-CYP2C8 antibody NBP1-88054 (A) shows similar pattern to independent antibody NBP1-88055 (B).](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Western-Blot-NBP1-88054-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Cytochrome P450 2C8 Antibody [NBP1-88054] - Analysis using Anti-CYP2C8 antibody NBP1-88054 (A) shows similar pattern to independent antibody NBP1-88055 (B).")
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0016.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0017.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human cerebellum shows no positivity in Purkinje cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0014.jpg)
![Immunohistochemistry-Paraffin: Cytochrome P450 2C8 Antibody [NBP1-88054] - Staining of human Fallopian tube shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Cytochrome-P450-2C8-Antibody-Immunohistochemistry-Paraffin-NBP1-88054-img0015.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Analysis in human spleen and skeletal muscle tissues using . Corresponding AIF-1/Iba1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0001.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Analysis in human spleen and skeletal muscle tissues using . Corresponding AIF-1/Iba1 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human spleen using AIF-1/Iba1 Antibody shows very strong membranous positivity in cells in red pulp.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human cerebral cortex using AIF-1/Iba1 Antibody shows strong membranous positivity in glial cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human kidney using AIF-1/Iba1 Antibody shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human lymphoid tissues using AIF-1/Iba1 Antibody shows very strong membranous positivity in non-germinal, and germinal center cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human placenta using AIF-1/Iba1 Antibody shows very strong cytoplasmic positivity in Hofbauer cells.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0005.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP2-14276] - Staining of human skeletal muscle using AIF-1/Iba1 Antibody shows very weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP2-14276-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: EPHX2 Antibody [NBP1-89534] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/EPHX2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89534-img0007.jpg "Immunocytochemistry/Immunofluorescence: EPHX2 Antibody [NBP1-89534] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.")
![Immunohistochemistry-Paraffin: EPHX2 Antibody [NBP1-89534] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/EPHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-89534-img0011.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody [NBP1-89534] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/EPHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-89534-img0009.jpg)
![Immunohistochemistry-Paraffin: EPHX2 Antibody [NBP1-89534] - Staining of human prostate shows no positivity in smooth muscle cells as expected.](http://images.novusbio.com/fullsize/EPHX2-Antibody-Immunohistochemistry-Paraffin-NBP1-89534-img0010.jpg)
![Western Blot: LRSAM1 Antibody [NBP1-82016] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-329](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Western-Blot-NBP1-82016-img0005.jpg "Western Blot: LRSAM1 Antibody [NBP1-82016] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-329")
![Immunocytochemistry/Immunofluorescence: LRSAM1 Antibody [NBP1-82016] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-82016-img0006.jpg)
![Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunohistochemistry-Paraffin-NBP1-82016-img0009.jpg)
![Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunohistochemistry-Paraffin-NBP1-82016-img0001.jpg)
![Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunohistochemistry-Paraffin-NBP1-82016-img0007.jpg)
![Immunohistochemistry-Paraffin: LRSAM1 Antibody [NBP1-82016] - Staining of human Fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/LRSAM1-Antibody-Immunohistochemistry-Paraffin-NBP1-82016-img0008.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Analysis in human fallopian tube and skeletal muscle tissues using NBP1-87203 antibody. Corresponding Phosphoserine phosphatase RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0016.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Analysis in human fallopian tube and skeletal muscle tissues using NBP1-87203 antibody. Corresponding Phosphoserine phosphatase RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Phosphoserine phosphatase Antibody [NBP1-87203] - Analysis in human cell lines A-431 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Western-Blot-NBP1-87203-img0009.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0012.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0013.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0014.jpg)
![Immunohistochemistry-Paraffin: Phosphoserine phosphatase Antibody [NBP1-87203] - Staining of human skeletal muscle shows no postivity in myocytes as expected.](http://images.novusbio.com/fullsize/Phosphoserine-phosphatase-Antibody-Immunohistochemistry-Paraffin-NBP1-87203-img0015.jpg)
![Immunohistochemistry-Paraffin: PLEC1 Antibody [NBP1-92275] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PLEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-92275-img0007.jpg "Immunohistochemistry-Paraffin: PLEC1 Antibody [NBP1-92275] - Staining of human lymph node.")
![Immunohistochemistry-Paraffin: PLEC1 Antibody [NBP1-92275] - Staining of human urinary bladder shows moderate cytoplasmic positivity in urothelial cells.](http://images.novusbio.com/fullsize/PLEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-92275-img0004.jpg)
![Immunohistochemistry-Paraffin: PLEC1 Antibody [NBP1-92275] - Staining of human colon.](http://images.novusbio.com/fullsize/PLEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-92275-img0005.jpg)
![Immunohistochemistry-Paraffin: PLEC1 Antibody [NBP1-92275] - Staining of human kidney.](http://images.novusbio.com/fullsize/PLEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-92275-img0006.jpg)
![Immunohistochemistry-Paraffin: PLEC1 Antibody [NBP1-92275] - Staining of human liver.](http://images.novusbio.com/fullsize/PLEC1-Antibody-Immunohistochemistry-Paraffin-NBP1-92275-img0008.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249374925.jpg "<b>Genetic Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Wild-type (WT) and HMOX1 knockout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249393543.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects secreted Heme Oxygenase 1 protein by immunohistochemical analysis. Sample: Paraffin-embedded cat spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-212024940885.jpg)
![Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-212024943288.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249413392.jpg)
![Immunohistochemistry-Paraffin: HO-1/HMOX1/HSP32 Antibody (HL1780) - Azide and BSA Free [NBP3-25498] - Heme Oxygenase 1 antibody [HL1780] detects Heme Oxygenase 1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody [HL1780] (NBP3-25498) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25498_rabbit-ho-1-hmox1-hsp32-mab-hl1780-azide-and-bsa-free-2120249415928.jpg)
![Western Blot: Cytochrome c Antibody (CTC05) [NBP2-44445] - Western Blot Analysis of heart tissue lysate using Cytochrome c Antibody (CTC05).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CTC05-Western-Blot-NBP2-44445-img0003.jpg "Western Blot: Cytochrome c Antibody (CTC05) [NBP2-44445] - Western Blot Analysis of heart tissue lysate using Cytochrome c Antibody (CTC05).")
![Immunohistochemistry-Paraffin: Cytochrome c Antibody (CTC05) [NBP2-44445] - Human Heart stained with Cytochrome C Monoclonal Antibody (CTC05).](http://images.novusbio.com/fullsize/Cytochrome-c-Antibody-CTC05-Immunohistochemistry-Paraffin-NBP2-44445-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77459-img0008.jpg "Immunocytochemistry/Immunofluorescence: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - HepG2 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).")
![Immunohistochemistry: HO-1/HMOX1/HSP32 Antibody [NBP1-77459] - Analysis of heme oxygenase mouse kidney using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/HO-1-HMOX1-HSP32-Antibody-Immunohistochemistry-NBP1-77459-img0007.jpg)
![Immunohistochemistry-Paraffin: Guanine deaminase Antibody [NBP2-16769] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/Guanine-deaminase-Antibody-Immunohistochemistry-Paraffin-NBP2-16769-img0002.jpg "Immunohistochemistry-Paraffin: Guanine deaminase Antibody [NBP2-16769] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:500 dilution.")
![Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody [NBP1-33064] - Paraffin-embedded BT483 xenograft.SHP2 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-Immunohistochemistry-Paraffin-NBP1-33064-img0011.jpg "Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody [NBP1-33064] - Paraffin-embedded BT483 xenograft.SHP2 antibody dilution: 1:500.")
![Western Blot: SHP-2/PTPN11 Antibody [NBP1-33064] - Sample (50 ug of whole cell lysate) A: Mouse brain 7. 5% SDS PAGE, antibody diluted at 1:500.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-Western-Blot-NBP1-33064-img0008.jpg)
![Western Blot: SHP-2/PTPN11 Antibody [NBP1-33064] - 50 ug rat brain lysate/extract 7. 5 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-Western-Blot-NBP1-33064-img0009.jpg)
![Immunohistochemistry-Paraffin: SHP-2/PTPN11 Antibody [NBP1-33064] - Paraffin-embedded A549 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-Immunohistochemistry-Paraffin-NBP1-33064-img0007.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: SHP-2/PTPN11 Antibody [NBP1-33064] - Wild-type (WT) and SHP2 knockout (KO) 293T cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33064_rabbit-polyclonal-shp-2-ptpn11-antibody-1610202419414556.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Western-Blot-H00023569-M01-img0001.jpg "Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - detection against Immunogen (38.1 KDa) .")
![Immunocytochemistry/Immunofluorescence: PADI4 Antibody (4D8) [H00023569-M01] - Analysis of monoclonal antibody to PADI4 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PADI4-Antibody-4D8-Immunocytochemistry-Immunofluorescence-H00023569-M01-img0004.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Detection of enzymatically active peptidyl arginine deiminase (PAD) in the synovial fluid (SF) of patients with rheumatoid arthritis (RA). A, Immunoblotting of albumin‐depleted SF samples from 8 RA patients shows presence of neutrophil elastase (NE), PAD4, & PAD2. B, PAD activity in the SF of patients with RA (n = 12) was significantly higher than that in the SF of patients with osteoarthritis (OA) (n = 9). C, PAD activity was observed in the OA SF & RA SF both at physiologic calcium concentrations & at higher calcium concentrations (5 mM). In B & C, symbols represent individual patients; horizontal bars show the median. D, PAD activity is significantly correlated with DNA levels in the SF of RA patients (n = 22). Data were analyzed using Spearman's rho test for correlation. ∗ = P < 0.05; ∗∗ = P < 0.01, by Mann‐Whitney U test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415482220.jpg)
![Western Blot: PADI4 Antibody (4D8) [H00023569-M01] - Release of peptidyl arginine deiminases (PADs) into the supernatant during NETosis, & detection of enzymatically active PADs. A, DNA release in peripheral blood neutrophils from healthy donors that were left unstimulated or stimulated with 25 mM phorbol myristate acetate (PMA) was assessed for up to 240 minutes after stimulation. Bars show the mean ± SD of 8 samples per group. B, For isolation of neutrophil extracellular traps, supernatants (SN) of unstimulated (unst.) or stimulated (stim.) neutrophils were collected. Subsequently, the cells were washed 3 times with RPMI medium (W1–W3), & stimulated cells were incubated in the absence or presence of DNase I; unstimulated cells treated with DNase I served as a control. Bars show the mean ± SD DNA concentration (conc) in 7 samples per group. C, Proteins (PAD2, PAD4, & neutrophil elastase [NE]) were precipitated from the same supernatants of unstimulated or stimulated cells as described in B & analyzed by Western blotting. Citrullinated proteins (citr.prot.) were detected using chemical modification & anti–modified citrulline antibody. PAD2 & PAD4 antibodies were tested for cross‐reactivity using human (hu) recombinant PAD4 (rPAD4) (250 ng) & human skeletal muscle tissue lysate (15 μg). One representative blot of 4 independent experiments is shown for each group. D, PAD activity in supernatants of unstimulated & stimulated cells was compared. Symbols represent individual donors (n = 10); bars show the median. ∗ = P < 0.05; ∗∗ = P < 0.01, by Wilcoxon's matched pairs signed rank test. AFU = arbitrary fluorescence units; NS = not significant. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26245941), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/h00023569-m01_mouse-monoclonal-padi4-antibody-4d8-310202415485135.jpg)