| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92668-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59483-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03486-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05935-50ul | |||
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-06014-img0009.jpg "Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody [NBP1-06014] - Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using AIF-1/Iba1 Antibody (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).")
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NBP1-06014] - Staining of paraffin embedded Human Cerebellum AIF-1/Iba1 Antibody at 4 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Immunohistochemistry-Paraffin-NBP1-06014-img0007.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody [NBP1-06014] - Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) with AIF-1:Iba1 Antibody, followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-Flow-Cytometry-NBP1-06014-img0008.jpg)
![Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Western-Blot-NB100-1503-img0008.jpg "Western Blot: Lipocalin-2/NGAL Antibody [NB100-1503] - (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - Analysis of LCN2 in human xenograft tumor. Image courtesy of product review submitted by Yi-Ling Huang.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Immunohistochemistry-Paraffin-NB100-1503-img0006.jpg)
![Immunohistochemistry-Paraffin: Lipocalin-2/NGAL Antibody [NB100-1503] - (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Lipocalin-2-NGAL-Antibody-Immunohistochemistry-Paraffin-NB100-1503-img0005.jpg)
![Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of various lysates, using ALOX15B Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-western-blot-2212025105133..png "Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of various lysates, using ALOX15B Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of NIH/3T3 cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-4220251425187.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of U2OS cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-42202514264439.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of PC-12 cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-42202514275258.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0009.jpg "Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The recombinat proteins (100ng) were resolved by SDS PAGE, transferred to PVDF membrane and probed with antihuman GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Recombinant human GOT1 Lane 2 : Recombinant human GOT2")
![Immunocytochemistry/Immunofluorescence: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Analysis of GOT1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of GOT1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Immunocytochemistry-Immunofluorescence-NBP2-59483-img0002.jpg)
![Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Flow-Cytometry-NBP2-59483-img0006.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Lane 1: 293T cell lysates, Lane 2: GOT1 Transfected 293T cell lysates](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0001.jpg)
![Western Blot: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : A427 cell lysate](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Western-Blot-NBP2-59483-img0008.jpg)
![Flow Cytometry: Aspartate Aminotransferase Antibody (3G11) [NBP2-59483] - Flow cytometry analysis of GOT1 in HeLa cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/Aspartate-Aminotransferase-Antibody-3G11-Flow-Cytometry-NBP2-59483-img0003.jpg)
![Western Blot: SHP-2/PTPN11 Antibody [NBP3-03486] - Analysis of extracts from normal (control) and PTPN11 knockout (KO) 293T cells, using SHP-2/PTPN11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-Western-Blot-NBP3-03486-img0002.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SHP-2/PTPN11 Antibody [NBP3-03486] - Analysis of extracts from normal (control) and PTPN11 knockout (KO) 293T cells, using SHP-2/PTPN11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Western Blot: SHP-2/PTPN11 Antibody [NBP3-03486] - Analysis of extracts of various cell lines, using SHP-2/PTPN11 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-Western-Blot-NBP3-03486-img0003.jpg)
![Immunoprecipitation: SHP-2/PTPN11 Antibody [NBP3-03486] - Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug SHP-2/PTPN11 antibody (NBP3-03486). Western blot was performed from the immunoprecipitate using SHP-2/PTPN11 antibody (NBP3-03486) at a dilution of 1:2000.](http://images.novusbio.com/fullsize/SHP-2-PTPN11-Antibody-Immunoprecipitation-NBP3-03486-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: SHP-2/PTPN11 Antibody - Azide and BSA Free [SHP-2/PTPN11] - Immunofluorescence analysis of C6 cells using [KO Validated] SHP-2/PTPN11 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03486_rabbit-polyclonal-shp-2-ptpn11-antibody-5220251634524.jpg)
![Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of extracts of various cell lines, using COX-2 antibody (NBP3-16220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/COX-2-Antibody-2V7G8-Western-Blot-NBP3-16220-img0002.jpg "Western Blot: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of extracts of various cell lines, using COX-2 antibody (NBP3-16220) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon carcinoma tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-155202485950..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Human colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249058..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] -Aanalysis of COX2/PTGS2 in paraffin-embedded mouse intestin tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249247..jpg)
![Immunohistochemistry-Paraffin: COX-2 Antibody (2V7G8) [NBP3-16220] - Analysis of COX2/PTGS2 in paraffin-embedded rat colon tissue using COX2/PTGS2 Rabbit mAb at a dilution of 1:200 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-16220_rabbit-cox-2-mab-2v7g8-immunohistochemistry-paraffin-15520249513.jpg)
![Western Blot: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Western blot analysis of extracts of various cell lines, using FABP1/L-FABP Rabbit mAb (NBP3-15353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-8Q8S10-Western-Blot-NBP3-15353-img0003.jpg "Western Blot: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Western blot analysis of extracts of various cell lines, using FABP1/L-FABP Rabbit mAb (NBP3-15353) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.")
![Immunocytochemistry/Immunofluorescence: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Immunofluorescence analysis of U-2 OS cells using FABP1/L-FABP Rabbit mAb (NBP3-15353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-8Q8S10-Immunocytochemistry-Immunofluorescence-NBP3-15353-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: FABP1/L-FABP Antibody (8Q8S10) [NBP3-15353] - Immunofluorescence analysis of NIH-3T3 cells using FABP1/L-FABP Rabbit mAb (NBP3-15353) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/FABP1-L-FABP-Antibody-8Q8S10-Immunocytochemistry-Immunofluorescence-NBP3-15353-img0001.jpg)
![Western Blot: FABP1/L-FABP Antibody (8Q8S10) [FABP1/L-FABP] - Western blot analysis of various lysates using FABP1/L-FABP Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-15353_rabbit-fabp1-l-fabp-mab-8q8s10-52202517192965.jpg)
![Western Blot: FABP1/L-FABP Antibody (8Q8S10) [FABP1/L-FABP] - Western blot analysis of lysates from Hep G2 cells using FABP1/L-FABP Rabbit mAb at 1:1000 dilution incubated at room temperature for 1.5 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-15353_rabbit-fabp1-l-fabp-mab-8q8s10-52202517284936.jpg)
![Western Blot: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:88 kDaObserved band size:83 kDa](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Western-Blot-NBP3-05935-img0001.jpg "Western Blot: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A: HeLa Whole Cell LysateLane B: NIH3T3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:88 kDaObserved band size:83 kDa")
![Immunohistochemistry-Paraffin: Phospholipase C delta 1 Antibody [NBP3-05935] - Staining of human PLCD1 in human kidney with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunohistochemistry-Paraffin-NBP3-05935-img0002.jpg)
![Immunoprecipitation: Phospholipase C delta 1 Antibody [NBP3-05935] - Lane A:0.5 mg NIH3T3 Whole Cell Lysate4 uL anti-PLCD1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PLCD1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 86 kDaObserved band size :90 kDa](http://images.novusbio.com/fullsize/Phospholipase-C-delta-1-Antibody-Immunoprecipitation-NBP3-05935-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Phospholipase C delta 1 Antibody [NBP3-05935] - Staining of PLCD1 in U251MG cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-05935 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-05935_rabbit-polyclonal-phospholipase-c-delta-1-antibody-235202511494035.png)
![Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-278202416285246.jpg "Western Blot: Granulin Antibody (JE37-73) [NBP3-32371] - Western blot analysis of Granulin on different lysates with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. Lane 1: HepG2 cell lysate Lane 2: HEK-293 cell lysate Lane 3: TF-1 cell lysate Lane 4: THP-1 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 64 kDa Observed band size: 50-75 kDa Exposure time: 1 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32371) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Granulin Antibody (JE37-73) [NBP3-32371] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Granulin antibody (NBP3-32371) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32371) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-298202414262694.jpg)
![Immunocytochemistry/ Immunofluorescence: Granulin Antibody (JE37-73) [NBP3-32371] - Immunocytochemistry analysis of THP-1 cells labeling Granulin with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Granulin antibody (NBP3-32371) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-2920241114832.jpg)
![Flow Cytometry: Granulin Antibody (JE37-73) [NBP3-32371] - Flow cytometric analysis of THP-1 cells labeling Granulin. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32371, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32371_rabbit-granulin-mab-je37-73-29202416453258.jpg)
![Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 expression in transfected 293T cell line by ALDH1A1 monoclonal antibody (M05), clone 1G6. Lane 1: ALDH1A1 transfected lysate (Predicted MW: 54.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-1G6-Western-Blot-H00000216-M05-img0004.jpg "Western Blot: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 expression in transfected 293T cell line by ALDH1A1 monoclonal antibody (M05), clone 1G6. Lane 1: ALDH1A1 transfected lysate (Predicted MW: 54.9 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of monoclonal antibody to ALDH1A1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-1G6-Immunohistochemistry-Paraffin-H00000216-M05-img0001.jpg)
![Immunoprecipitation: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Analysis of ALDH1A1 transfected lysate using anti-ALDH1A1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ALDH1A1 rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-1G6-Immunoprecipitation-H00000216-M05-img0002.jpg)
![ELISA: Aldehyde Dehydrogenase 1-A1/ALDH1A1 Antibody (1G6) [H00000216-M05] - Detection limit for recombinant GST tagged ALDH1A1 is 1 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Aldehyde-Dehydrogenase-1-A1-ALDH1A1-Antibody-1G6-ELISA-H00000216-M05-img0005.jpg)
![Western Blot: ECH1 Antibody [NBP1-31662] - Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP1-31662-img0005.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ECH1 Antibody [NBP1-31662] - Wild-type (WT) and ECH1 knockout (KO) 293T cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ECH1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-31662] - Sample: Paraffin-embedded mouse intestine. ECH1 antibody diluted at 1:500.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunohistochemistry-Paraffin-NBP1-31662-img0004.jpg)
![Western Blot: ECH1 Antibody [NBP1-31662] - Sample (30 ug of whole cell lysate) A: 293T B: Hep G2 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/ECH1-Antibody-Western-Blot-NBP1-31662-img0003.jpg)
![Immunohistochemistry-Paraffin: ECH1 Antibody [NBP1-31662] - Paraffin-embedded OV90 xenograft, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/ECH1-Antibody-Immunohistochemistry-Paraffin-NBP1-31662-img0002.jpg)
![Western Blot: MVD Antibody [NBP1-33050] - A. 30 ug 293T whole lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug A37C whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.](http://images.novusbio.com/fullsize/MVD-Antibody-Western-Blot-NBP1-33050-img0006.jpg "Western Blot: MVD Antibody [NBP1-33050] - A. 30 ug 293T whole lysate/extract, B. 30 ug A431 whole cell lysate/extract, C. 30 ug HeLa whole cell lysate/extract. D. 30 ug A37C whole cell lysate/extract 10 % SDS-PAGE gel, antibody dilution 1:1000.")
![Immunocytochemistry/Immunofluorescence: MVD Antibody [NBP1-33050] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MVD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33050-img0003.jpg)
![Immunohistochemistry-Paraffin: MVD Antibody [NBP1-33050] - Paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/MVD-Antibody-Immunohistochemistry-Paraffin-NBP1-33050-img0004.jpg)
![Western Blot: MVD Antibody [NBP1-33050] - Sample (50 ug of whole cell lysate) A: mouse Liver 10% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MVD-Antibody-Western-Blot-NBP1-33050-img0005.jpg)