| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71525 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38154-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92202-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-59390-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89830-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-55293SS | |||
![Western Blot: CAR/NR1I3 Antibody (1C6) [NBP2-46226] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3.](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-1C6-Western-Blot-NBP2-46226-img0005.jpg "Western Blot: CAR/NR1I3 Antibody (1C6) [NBP2-46226] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3.")
![Immunohistochemistry: CAR/NR1I3 Antibody (1C6) [NBP2-46226] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-1C6-Immunohistochemistry-NBP2-46226-img0004.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (1C6) [NBP2-46226] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-1C6-Immunohistochemistry-NBP2-46226-img0001.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (1C6) [NBP2-46226] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-1C6-Immunohistochemistry-NBP2-46226-img0002.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (1C6) [NBP2-46226] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-1C6-Immunohistochemistry-NBP2-46226-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ACSL5 Antibody [NB100-2918] - Staining of Human Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Western-Blot-NB100-2918-img0005.jpg)
![Immunohistochemistry-Paraffin: ACSL5 Antibody [NB100-2918] - Staining of human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/ACSL5-Antibody-Immunohistochemistry-Paraffin-NB100-2918-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: ERAB Antibody [NBP3-38154] - Immunofluorescence analysis of U-2 OS cells using ERAB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38154_rabbit-erab-pab-31120251704751.jpg "Immunocytochemistry/ Immunofluorescence: ERAB Antibody [NBP3-38154] - Immunofluorescence analysis of U-2 OS cells using ERAB Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: ERAB Antibody [NBP3-38154] - Western Blot analysis of various lysates using ERAB Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38154_rabbit-erab-pab-3112025173125.jpg)
![Immunohistochemistry: ERAB Antibody [NBP3-38154] - Immunohistochemistry analysis of paraffin-embedded Human colon using ERAB/ERAB/HSD17B10 Rabbit pAb (A5448) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/nbp3-38154_rabbit-erab-pab-1012202411552814.jpg)
![Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Western-Blot-NBP2-92202-img0002.jpg "Western Blot: ACSS1 Antibody [NBP2-92202] - Analysis of extracts of various cell lines, using ACSS1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ACSS1 Antibody [NBP2-92202] - Analysis of L929 cells using ACSS1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-92202-img0001.jpg)
![Immunohistochemistry-Paraffin: ACSS1 Antibody [NBP2-92202] - Immunohistochemistry of paraffin-embedded Human colon carcinoma using ACSS1 Rabbit pAb (NBP2-92202) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/ACSS1-Antibody-Immunohistochemistry-Paraffin-NBP2-92202-img0003.jpg)
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0004.jpg "Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - The mouse adipose tissue lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ATGL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection syste")
![Immunocytochemistry/Immunofluorescence: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis of ATGL in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). Analysis of ATGL in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human ATGL antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Immunocytochemistry-Immunofluorescence-NBP2-59390-img0002.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in HeLa cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0006.jpg)
![Western Blot: PEDF R/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Analysis in A431 cell lysates (40ug), resolved by SDS-PAGE.](http://images.novusbio.com/fullsize/PEDF-R-PNPLA2-ATGL-Antibody-AT18E6-Western-Blot-NBP2-59390-img0001.jpg)
![Flow Cytometry: PEDFR/PNPLA2/ATGL Antibody (AT18E6) [NBP2-59390] - Flow cytometry analysis of ATGL in Hep3B cells. The cell was stained with NBP2-59390 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).](http://images.novusbio.com/fullsize/PEDFR-PNPLA2-ATGL-Antibody-AT18E6-Flow-Cytometry-NBP2-59390-img0005.jpg)
![Western Blot: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 4A (CYP4A11) Rabbit mAb (NBP3-15847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Cytochrome-P450-4A-Antibody-10D10W7-Western-Blot-NBP3-15847-img0004.jpg "Western Blot: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Western blot analysis of extracts of various cell lines, using Cytochrome P450 4A (CYP4A11) Rabbit mAb (NBP3-15847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded mouse kidney tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15847_rabbit-cytochrome-p450-4a-mab-10d10w7-72202512435322.jpg)
![Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded rat kidney tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15847_rabbit-cytochrome-p450-4a-mab-10d10w7-7220251245339.jpg)
![Immunohistochemistry: Cytochrome P450 4A Antibody (10D10W7) [NBP3-15847] - Immunohistochemistry analysis of Cytochrome P450 4A in paraffin-embedded human liver tissue using Cytochrome P450 4A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15847_rabbit-cytochrome-p450-4a-mab-10d10w7-72202512435385.jpg)
![Western Blot: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3.](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Western-Blot-NBP2-71525-img0001.jpg "Western Blot: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR1I3.")
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0004.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0002.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0003.jpg)
![Immunohistochemistry: CAR/NR1I3 Antibody (OTI1C6) - Azide and BSA Free [NBP2-71525] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/CAR-NR1I3-Antibody-OTI1C6-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71525-img0005.jpg)
![Western Blot: FABP2/I-FABP Antibody (OTI2C4) - Azide and BSA Free [NBP2-70699] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY intestinal FABP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-intest](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-OTI2C4-Azide-and-BSA-Free-Western-Blot-NBP2-70699-img0001.jpg "Western Blot: FABP2/I-FABP Antibody (OTI2C4) - Azide and BSA Free [NBP2-70699] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY intestinal FABP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-intest")
![Immunohistochemistry: FABP2/I-FABP Antibody (OTI2C4) - Azide and BSA Free [NBP2-70699] - Staining of paraffin-embedded Human colon tissue using anti-intestinal FABP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-OTI2C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70699-img0003.jpg)
![Flow Cytometry: FABP2/I-FABP Antibody (OTI2C4) - Azide and BSA Free [NBP2-70699] - intestinal FABP Antibody (2C4) [NBP2-01239](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-OTI2C4-Azide-and-BSA-Free-Flow-Cytometry-NBP2-70699-img0002.jpg)
![Immunohistochemistry: FABP2/I-FABP Antibody (OTI2C4) - Azide and BSA Free [NBP2-70699] - Staining of paraffin-embedded Human pancreas tissue using anti-intestinal FABP mouse monoclonal antibody.](http://images.novusbio.com/fullsize/FABP2-I-FABP-Antibody-OTI2C4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-70699-img0004.jpg)
![Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa](http://images.novusbio.com/fullsize/CD39L4-ENTPD5-Antibody-064-Western-Blot-NBP2-89830-img0001.jpg "Western Blot: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Anti-CD39L4/ENTPD5 rabbit monoclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lysates/proteins at 30 µg per lane. Secondary: Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 53 kDa")
![Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human liver with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-235202511545677.png)
![Immunohistochemistry-Paraffin: CD39L4/CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - Immunochemical staining of human CD39L4/ENTPD5 in human kidney with NBP2-89830 at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-235202511444489.png)
![Immunoprecipitation: CD39L4/ENTPD5 Antibody (064) [NBP2-89830] - ENTPD5 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 0.5 uL NBP2-89830 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP2-89830 at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 47 kDa Observed band size: 47 kDa](http://images.novusbio.com/fullsize/nbp2-89830_rabbit-monoclonal-cd39l4-entpd5-antibody-064-23520251154565.png)
![Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-278202415295516.jpg "Western Blot: HADH Antibody (D10-E7-R) [NBP3-32416] - Western blot analysis of HADH on different lysates with Mouse anti-HADH antibody (NBP3-32416) at 1/1,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: HT-29 cell lysate (20 ug/Lane) Lane 3: HL-60 cell lysate (20 ug/Lane) Lane 4: A431 cell lysate (20 ug/Lane) Lane 5: K-562 cell lysate (20 ug/Lane) Lane 6: Human liver tissue lysate (40 ug/Lane) Lane 7: Mouse liver tissue lysate (40 ug/Lane) Lane 8: Mouse heart tissue lysate (40 ug/Lane) Lane 9: Rat liver tissue lysate (40 ug/Lane) Lane 10: Rat heart tissue lysate (40 ug/Lane) Predicted band size: 34 kDa Observed band size: 34 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-HADH antibody (NBP3-32416) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32416) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29820241420931.jpg)
![Immunocytochemistry/ Immunofluorescence: HADH Antibody (D10-E7-R) [NBP3-32416] - Immunocytochemistry analysis of HeLa cells labeling HADH with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HADH antibody (NBP3-32416) at 1/150 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32416_mouse-hadh-mab-d10-e7-r-29202410334032.jpg)
![Western Blot: PGC-1 beta Antibody [NBP1-28722] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PGC-1beta antibody NBP1-28722 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. PGC-1beta was also immunoprecipitated by rabbit anti-PGC-1beta antibody NBP1-28721, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).](http://images.novusbio.com/fullsize/PGC-1-beta-Antibody-Western-Blot-NBP1-28722-img0001.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PGC-1 beta Antibody [NBP1-28722] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PGC-1beta antibody NBP1-28722 used for WB at 0.4 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. PGC-1beta was also immunoprecipitated by rabbit anti-PGC-1beta antibody NBP1-28721, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).")
![<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: PGC-1 beta Antibody [NBP1-28722] - Whole cell lysate (50 ug) from HeLa, HEK293T, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PGC-1beta antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/antibody/nbp1-28722_rabbit-polyclonal-pgc-1-beta-antibody-western-blot-286202382436.jpg)
![Western Blot: GPIHBP1 Antibody [NB110-55293] - Detection of HDLBP in CHO transfected lysate using NB110-55293. Lane 1: empty vector Lane 2: HDLBP transfected lysate](http://images.novusbio.com/fullsize/GPIHBP1-Antibody-Western-Blot-NB110-55293-img0002.jpg "Western Blot: GPIHBP1 Antibody [NB110-55293] - Detection of HDLBP in CHO transfected lysate using NB110-55293. Lane 1: empty vector Lane 2: HDLBP transfected lysate")