| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71059 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12091-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-64798-0.05mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76799-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-41209-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76740SS | |||
![Western Blot: TAK1 Antibody [NBP3-13401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP3-13401-img0004.jpg "Western Blot: TAK1 Antibody [NBP3-13401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: TAK1 Antibody [NBP3-13401] - TAK1 antibody detects TAK1 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TAK1 stained by TAK1 antibody (NBP3-13401) diluted at 1:500. Blue: Fluoroshield with DAPI. Scale bar= 10 um.](http://images.novusbio.com/fullsize/TAK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13401-img0005.jpg)
![Western Blot: TAK1 Antibody [NBP3-13401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP3-13401-img0001.jpg)
![Western Blot: TAK1 Antibody [NBP3-13401] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP3-13401-img0002.jpg)
![Western Blot: TAK1 Antibody [NBP3-13401] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TAK1 antibody (NBP3-13401) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/TAK1-Antibody-Western-Blot-NBP3-13401-img0003.jpg)
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0002.jpg "Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0004.jpg)
![Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysate](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in Balb/3T3 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0003.jpg)
![Western Blot: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of Human and Mouse INPPL2/SHIP2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-Western-Blot-NB100-1592-img0011.jpg "<b>Biological Strategies Validation. </b>Western Blot: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of Human and Mouse INPPL2/SHIP2 by Western Blot. Samples: Whole cell lysate (50, 15, 5 ug) from HeLa and (50 ug) from 293T and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: SHIP2/INPPL1 Antibody [NB100-1592] - Sample: FFPE serial sections of human colon carcinoma. Antibody: Affinity purified rabbit anti-expndtw-6 INPPL1/SHIP2 Cat. No. Lot 1 (left) and Lot 2 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-Immunohistochemistry-NB100-1592-img0009.jpg)
![Immunoprecipitation: SHIP2/INPPL1 Antibody [NB100-1592] - Detection of human INPPL2/SHIP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-INPPL2/SHIP2 antibody NB100-1592 (lot NB100-1592-2) used for IP at 6 ug per reaction. INPPL2/SHIP2 was also immunoprecipitated by a previous lot of this antibody (NB100-1592-1) and another rabbit anti-INPPL2/SHIP2 antibody. For blotting immunoprecipitated INPPL2/SHIP2, NB100-1592 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-Antibody-NB100-1592-img0012.jpg)
![Western Blot: Jun Antibody (3G2) [NBP2-02258] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jun (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Jun.](http://images.novusbio.com/fullsize/Jun-Antibody-3G2-Western-Blot-NBP2-02258-img0004.jpg "Western Blot: Jun Antibody (3G2) [NBP2-02258] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Jun (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Jun.")
![Immunocytochemistry/Immunofluorescence: Jun Antibody (3G2) [NBP2-02258] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Jun.](http://images.novusbio.com/fullsize/Jun-Antibody-3G2-Immunocytochemistry-Immunofluorescence-NBP2-02258-img0005.jpg)
![Flow Cytometry: Jun Antibody (3G2) [NBP2-02258] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Jun antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Jun-Antibody-3G2-Flow-Cytometry-NBP2-02258-img0003.jpg)
![Flow Cytometry: Jun Antibody (3G2) [NBP2-02258] - Analysis of Hela cells, using anti-Jun antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Jun-Antibody-3G2-Flow-Cytometry-NBP2-02258-img0001.jpg)
![Flow Cytometry: Jun Antibody (3G2) [NBP2-02258] - Analysis of Jurkat cells, using anti-Jun antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/Jun-Antibody-3G2-Flow-Cytometry-NBP2-02258-img0002.jpg)
![Flow Cytometry: CD90/Thy1 Antibody (5E10) [NBP1-43379] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43379. Staining of the HEL cell line with Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (open histogram) or Anti-Human CD90 (Thy-1) PerCP-Cy5.5 (filled histogram). Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-5E10-Flow-Cytometry-NBP1-43379-img0003.jpg "Flow Cytometry: CD90/Thy1 Antibody (5E10) [NBP1-43379] - Analysis using the PerCP/Cy5.5 conjugate of NBP1-43379. Staining of the HEL cell line with Mouse IgG1 kappa Isotype Control PerCP-Cy5.5 (open histogram) or Anti-Human CD90 (Thy-1) PerCP-Cy5.5 (filled histogram). Total viable cells were used for analysis.")
![Flow Cytometry: CD90/Thy1 Antibody (5E10) [NBP1-43379] - Staining of human erythroleukemia (HEL) cell line with 0.25 ug of Mouse IgG1 kappa Isotype Control Purified (NBP1-43319) (open histogram) or 0.25 ug of Anti-Human CD90 (Thy-1) Purified (filled histogram) followed by Anti-Mouse IgG FITC. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/CD90-Thy1-Antibody-5E10-Flow-Cytometry-NBP1-43379-img0001.jpg)
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IF staining of human kidney with NBP3-12091. Strong staining of veins of nephritis kidney. Dilution: 1:200](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0004.jpg "Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IF staining of human kidney with NBP3-12091. Strong staining of veins of nephritis kidney. Dilution: 1:200")
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IHC staining of human kidney with NBP3-12091. Strong staining of veins of nephritis kidney. Dilution: 1:600](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0001.jpg)
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IHC staining of human liver with NBP3-12091. Cytoplasm of hepatocytes moderately labelled and plasma of veins strongly in liver tissue. Dilution: 1:600](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0002.jpg)
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IHC staining in human tonsil with NBP3-12091. Plasma in lumen of veins is stained strongly as well as follicular dendritic cells in germinal center of tonsil. Dilution: 1:600](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0003.jpg)
![Immunohistochemistry: Complement C4c Antibody (99-72-18) - BSA Free [NBP3-12091] - IF staining in human tonsil with NBP3-12091. Plasma in lumen of veins is stained strongly in tonsil tissue. Dilution: 1:200](http://images.novusbio.com/fullsize/Complement-C4c-Antibody-99-72-18-BSA-Free-Immunohistochemistry-NBP3-12091-img0005.jpg)
![ELISA: Mouse Caspase-1 ELISA Kit (Colorimetric) [NBP2-75014] - Samples were spiked with high concentrations of Mouse Caspase-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-75014_mouse-caspase-1-elisa-kit-colorimetric-13220241692273.jpg "ELISA: Mouse Caspase-1 ELISA Kit (Colorimetric) [NBP2-75014] - Samples were spiked with high concentrations of Mouse Caspase-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse Caspase-1 ELISA Kit (Colorimetric) [NBP2-75014] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-75014_mouse-caspase-1-elisa-kit-colorimetric-4320248582540.jpg)
![Immunocytochemistry/Immunofluorescence: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of expression of IL-4 and IL-4 type II receptor subunits in undifferentiated mouse ESCs and mouse primary myoblasts. Representative images of immunocytochemistry analysis of IL-4Ralpha, IL-13Ralpha1, and IL-4 in mouse primary myoblasts at day 6 (white arrows indicate myoblasts, grey arrows indicate myotubes). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/16/3932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-4-Antibody-BVD4-1D11-Immunocytochemistry-Immunofluorescence-NB100-64798-img0003.jpg "Immunocytochemistry/Immunofluorescence: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of expression of IL-4 and IL-4 type II receptor subunits in undifferentiated mouse ESCs and mouse primary myoblasts. Representative images of immunocytochemistry analysis of IL-4Ralpha, IL-13Ralpha1, and IL-4 in mouse primary myoblasts at day 6 (white arrows indicate myoblasts, grey arrows indicate myotubes). Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/20/16/3932), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of expression of IL-4 and IL-4 type II receptor subunits in undifferentiated mouse ESCs and mouse primary myoblasts. Representative images of immunocytochemistry analysis of IL-4Ralpha, IL-13Ralpha1, and IL-4 in undifferentiated ESCs. Scale bar: 20 um. Scale bar: 20 um. Image collected and cropped by CiteAb from the following publication (mdpi.com/1422-0067/20/16/3932), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/IL-4-Antibody-BVD4-1D11-Immunocytochemistry-Immunofluorescence-NB100-64798-img0004.jpg)
![Western Blot: IL-4 Antibody (BVD4-1D11) [NB100-64798] - Analysis of mouse IL-4 recombinant protein probed with RAT ANTI MOUSE INTERLEUKIN-4 followed by F(ab')2 RABBIT ANTI RAT IgG:HRP.](http://images.novusbio.com/fullsize/IL-4-Antibody-BVD4-1D11-Western-Blot-NB100-64798-img0002.jpg)
![Western Blot: IKK gamma Antibody [NBP1-76799] - Analysis of IKKg in HeLa whole cell lysate with anti-IKKg/NEMO using NBP1-76799 at 1 ug/ml.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Western-Blot-NBP1-76799-img0003.jpg "Western Blot: IKK gamma Antibody [NBP1-76799] - Analysis of IKKg in HeLa whole cell lysate with anti-IKKg/NEMO using NBP1-76799 at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody - BSA Free [NBP1-76799] - Immunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 u/mL.Red: IKK gamma Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76799_rabbit-polyclonal-ikk-gamma-antibody-2711202420175134.gif)
![Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody - BSA Free [NBP1-76799] - Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76799_rabbit-polyclonal-ikk-gamma-antibody-2711202419261832.jpg)
![Western Blot: CCL2/MCP1 Antibody [NBP2-41209] - Analysis of CCL2 in K562 cell lysate with CCL2 antibody at 1 ug/mL.](http://images.novusbio.com/fullsize/CCL2-MCP1-Antibody-Western-Blot-NBP2-41209-img0005.jpg "Western Blot: CCL2/MCP1 Antibody [NBP2-41209] - Analysis of CCL2 in K562 cell lysate with CCL2 antibody at 1 ug/mL.")
![Immunohistochemistry: CCL2/MCP1 Antibody - BSA Free [NBP2-41209] - Immunohistochemistry of CCL2/MCP1 in human spleen tissue with CCL2/MCP1 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41209_rabbit-polyclonal-ccl2-mcp1-antibody-2711202420414544.jpg)
![Immunocytochemistry/ Immunofluorescence: CCL2/MCP1 Antibody - BSA Free [NBP2-41209] - Immunofluorescence of CCL2/MCP1 in human spleen tissue with CCL2/MCP1 antibody at 20 ug/ml.](http://images.novusbio.com/fullsize/nbp2-41209_rabbit-polyclonal-ccl2-mcp1-antibody-27112024200419.jpg)
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0007.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Expression of Tagged Proteins Validation. </b>Immunocytochemistry/Immunofluorescence: IL-21 Antibody [NBP1-76740] - Immunocytochemistry Validation of IL-21 in K562 Cells.Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (NBP1-76740) at 2 ug/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IL-21-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76740-img0008.jpg)
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740 (1 ug/mL), IL-21 from Company B (5 ug/mL), beta-actin (5 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0005.jpg)
![Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Figure 3 Western Blot Validation in Human HL-60 Cell LysateLoading: 15 u of lysates per lane.Antibodies: IL-21 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-76740_rabbit-polyclonal-il-21-antibody-271120241937752.jpg)