| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34039-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72069 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97564-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27224 | |||
| BSA Free | NBP2-27223SS | |||
| Azide and BSA Free | NBP2-27224SS | |||
![Immunohistochemistry-Paraffin: CD8 Antibody [NBP2-34039] - CD8 alpha Antibody [NBP2-34039] - Analysis in human spleen and skeletal muscle tissues. Corresponding CD8A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 Antibody [NBP2-34039] - CD8 alpha Antibody [NBP2-34039] - Analysis in human spleen and skeletal muscle tissues. Corresponding CD8A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 alpha Antibody [NBP2-34039] - Staining of human spleen shows moderate to strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0003.jpg)
![Immunohistochemistry-Paraffin: CD8 alpha Antibody [NBP2-34039] - Staining of human lymph node shows moderate to strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0004.jpg)
![Immunohistochemistry-Paraffin: CD8 alpha Antibody [NBP2-34039] - Staining of human small intestine shows moderate to strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0005.jpg)
![Immunohistochemistry-Paraffin: CD8 alpha Antibody [NBP2-34039] - Staining of human skeletal muscle shows no cytoplasmic positivity as expected.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-34039-img0006.jpg)
![Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Western-Blot-NBP3-14749-img0003.jpg "Western Blot: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Whole cell lysate (25 ug) from HeLa, Daudi, U2OS, SUP-T1, and Jurkat cells prepared using NETN lysis buffer. Rabbit anti-CD127 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.")
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Immunohistochemistry-NBP3-14749-img0002.jpg)
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749] - Rabbit anti-CD127/IL-7R alpha recombinant monoclonal antibody. HRP-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/IL-7R-alpha-CD127-Antibody-BLR177J-Immunohistochemistry-NBP3-14749-img0001.jpg)
![Immunohistochemistry: IL-7R alpha/CD127 Antibody (BLR177J) [NBP3-14749]- Rabbit anti-CD127/IL-7R alpha recombinantmonoclonal antibody [BLR177J] Secondary: HRP-conjugated goat anti-rabbit IgG .Substrate: DAB.](http://images.novusbio.com/fullsize/nbp3-14749_rabbit-il-7r-alpha-cd127-mab-immunohistochemistry-paraffin-1012024122450..jpg)
![Western Blot: Latexin Antibody (OTI1E10) [NBP2-01382] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LXN.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Western-Blot-NBP2-01382-img0001.jpg "Western Blot: Latexin Antibody (OTI1E10) [NBP2-01382] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LXN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LXN.")
![Immunohistochemistry-Paraffin: Latexin Antibody (OTI1E10) [NBP2-01382] - Staining of paraffin-embedded Human lymphoma tissue using anti-LXN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Immunohistochemistry-Paraffin-NBP2-01382-img0009.jpg)
![Flow Cytometry: Latexin Antibody (OTI1E10) [NBP2-01382] - HEK293T cells transfected with either Latexin Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LXN antibody , and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Flow-Cytometry-NBP2-01382-img0010.jpg)
![Immunohistochemistry-Paraffin: Latexin Antibody (OTI1E10) [NBP2-01382] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-LXN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Immunohistochemistry-Paraffin-NBP2-01382-img0002.jpg)
![Immunohistochemistry-Paraffin: Latexin Antibody (OTI1E10) [NBP2-01382] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-LXN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Immunohistochemistry-Paraffin-NBP2-01382-img0003.jpg)
![Immunohistochemistry-Paraffin: Latexin Antibody (OTI1E10) [NBP2-01382] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-LXN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Immunohistochemistry-Paraffin-NBP2-01382-img0004.jpg)
![Immunohistochemistry-Paraffin: Latexin Antibody (OTI1E10) [NBP2-01382] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-LXN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Immunohistochemistry-Paraffin-NBP2-01382-img0005.jpg)
![Immunohistochemistry-Paraffin: Latexin Antibody (OTI1E10) [NBP2-01382] - Staining of paraffin-embedded Human endometrium tissue using anti-LXN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Immunohistochemistry-Paraffin-NBP2-01382-img0006.jpg)
![Immunohistochemistry-Paraffin: Latexin Antibody (OTI1E10) [NBP2-01382] - Staining of paraffin-embedded Human Kidney tissue using anti-LXN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Immunohistochemistry-Paraffin-NBP2-01382-img0007.jpg)
![Immunohistochemistry-Paraffin: Latexin Antibody (OTI1E10) [NBP2-01382] - Staining of paraffin-embedded Human lymph node tissue using anti-LXN mouse monoclonal antibody.](http://images.novusbio.com/fullsize/Latexin-Antibody-OTI1E10-Immunohistochemistry-Paraffin-NBP2-01382-img0008.jpg)
![Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0005.jpg "Western Blot: MIF Antibody [NB100-1789] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.")
![Western Blot: MIF Antibody [NB100-1789] - Staining of Daudi (A) and Jurkat (B) lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NB100-1789-img0003.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NB100-1789] - Staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Antibody at 5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NB100-1789-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: MIF Antibody [NB100-1789] - Co-localization of CXCR4 & MIF in urothelium.Representative sections from rats treated with saline (A–C) or CYP (D–I) are shown. The figure shows MIF immunostaining (green immunofluorescence), CXCR4 immunostaining (red immunofluorescence) & an overlay panel combining both immunostaining & a DAPI nuclear stain. MIF immunostaining is seen in basal & intermediate cells & in fibroblasts in the lamina propria of saline treated rats (A), while superficial cells do not stain for MIF. Arrows show luminal edge of urothelium. CXCR4 is restricted to basal & intermediate cells of urothelium (B) & lamina propria is not stained. Overlay of these panels (C) demonstrate co-localization of MIF & CXCR4 as orange coloring of cells. CYP treatment resulted in superficial cell staining for MIF (D,G) & CXCR4 (E,H) & overlay panels (F,I) demonstrate co-localization as orange color in urothelial cells. Arrows point to superficial cells showing MIF-CXCR4 co-localization. Calibration bar = 50 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/19066630), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1789_goat-polyclonal-mif-antibody-31020241554531.jpg)
![Western Blot: Bim Antibody (SU0318) [NBP2-67456] - Analysis of Bim on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Western-Blot-NBP2-67456-img0006.jpg "Western Blot: Bim Antibody (SU0318) [NBP2-67456] - Analysis of Bim on mouse thymus tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Immunocytochemistry-Immunofluorescence-NBP2-67456-img0003.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody (SU0318) [NBP2-67456] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Bim antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Immunohistochemistry-Paraffin-NBP2-67456-img0004.jpg)
![Western Blot: Bim Antibody (SU0318) [NBP2-67456] - Analysis of Bim on different lysates using anti-Bim antibody at 1/1,000 dilution. Positive control: Lane 1: Raji Lane 2: Jurkat Lane 3: A431](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Western-Blot-NBP2-67456-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Immunocytochemistry-Immunofluorescence-NBP2-67456-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Bim Antibody (SU0318) [NBP2-67456] - Staining Bim in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Bim-Antibody-SU0318-Immunocytochemistry-Immunofluorescence-NBP2-67456-img0002.jpg)
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-BSA-Free-Flow-Cytometry-NBP1-97564-img0005.jpg "Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Separation of human CD103 positive cells (red-filled) from CD103 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).")
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on human peripheral blood cells with anti-CD103 (Ber-ACT8) APC.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-Flow-Cytometry-NBP1-97564-img0001.jpg)
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) FITC.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-Flow-Cytometry-NBP1-97564-img0002.jpg)
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) [NBP1-97564] - Staining of CD103 on PHA-activated PBMC with anti-CD103 (Ber-ACT8) PE.](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-Flow-Cytometry-NBP1-97564-img0003.jpg)
![Flow Cytometry: Integrin alpha E/CD103 Antibody (Ber-ACT8) - BSA Free [NBP1-97564] - Staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD103 (Ber-ACT8) purified antibody (concentration in sample 3 ug/ml, GAM APC).](http://images.novusbio.com/fullsize/Integrin-alpha-E-CD103-Antibody-Ber-ACT8-BSA-Free-Flow-Cytometry-NBP1-97564-img0004.jpg)
![Western Blot: Leukotriene B4 R1 Antibody [NBP2-27422] - Analysis using BLT1 antibody. Lysate from human muscle in the 1) absence, 2) presence of immunizing peptide probed with BLT1 antibody at 3 ug/ml.](http://images.novusbio.com/fullsize/Leukotriene-B4-R1-Antibody-Western-Blot-NBP2-27422-img0004.jpg "Western Blot: Leukotriene B4 R1 Antibody [NBP2-27422] - Analysis using BLT1 antibody. Lysate from human muscle in the 1) absence, 2) presence of immunizing peptide probed with BLT1 antibody at 3 ug/ml.")
![Immunohistochemistry-Paraffin: Leukotriene B4 R1 Antibody [NBP2-27422] - Analysis of human liver using BLT1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/Leukotriene-B4-R1-Antibody-Immunohistochemistry-Paraffin-NBP2-27422-img0003.jpg)
![Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/Bim-Antibody-Western-Blot-NBP2-53088-img0001.jpg "Western Blot: Bim Antibody [NBP2-53088] - Total protein from human K562, HeLa, A549, HepG2 cells and mouse Raw264.7 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Bim/BCL2L11 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Bim Antibody [NBP2-53088] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-Bim at a 1:50 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-53088-img0003.jpg)
![Immunohistochemistry-Paraffin: Bim Antibody [NBP2-53088] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human endometrial cancer xenograft using Bim antibody at 1:50 dilution. The signal was detected using HRP labeled secondary antibody and DAB staining method which followed counter-staining with hematoxylin. The antibody generated a specific cytoplasmic staining with punctate appearance, signifying Bim/Bcl2L11 presence on mitochondria.](http://images.novusbio.com/fullsize/Bim-Antibody-Immunohistochemistry-Paraffin-NBP2-53088-img0002.jpg)
![Western Blot: OTUD5 Antibody [NBP2-24634] - Analysis of DUBA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/OTUD5-Antibody-Western-Blot-NBP2-24634-img0002.jpg "Western Blot: OTUD5 Antibody [NBP2-24634] - Analysis of DUBA in human liver lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse liver lysate and 4) rat liver lysate using this antibody. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Immunohistochemistry-Paraffin: OTUD5 Antibody [NBP2-24634] - Analysis of DUBA in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/OTUD5-Antibody-Immunohistochemistry-Paraffin-NBP2-24634-img0001.jpg)
![Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Western-Blot-NBP2-24461-img0004.jpg "Western Blot: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 (Y497) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 497 and 2) an unphosphorylated tyrosine at position 497, using SHIP2 phospho antibody at 0.01 ug/ml.")
![Immunohistochemistry-Paraffin: SHIP2/INPPL1 [p Tyr497] Antibody [NBP2-24461] - Analysis of phosphorylated SHIP2 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize/SHIP2-INPPL1-[p-Tyr497]-Antibody-Immunohistochemistry-Paraffin-NBP2-24461-img0003.jpg)
![Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Western-Blot-NBP2-27223-img0004.jpg "Western Blot: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Analysis using Azide Free version of NBP2-27223. TNF alpha recombinant protein using TNF alpha antibody at 3 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![<b>Biological Strategies Validation. </b>Flow Cytometry: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Human PBMC were stimulated with PMA (10 ng/mL) and Ionomycin (500 ng/mL) for 5h in presence of Brefeldin A. At the end of stimulation, cells were surface stained before testing by intracellular staining. Fixed and permeabilized cells were incubated with 10 uL (0.5 ug) of TNF- alpha antibody for 30 minutes on ice. Lymphocyte population was gated for analysis to show TNF alpha positive T cells.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Flow-Cytometry-NBP2-27223-img0002.jpg)
![<b>Biological Strategies Validation. </b>Inhibition Activity: TNF-alpha Antibody (6N1E7) [NBP2-27223] - Validation of inhibitory effects of TNF alpha antibody on TNF alpha activity in the cell line. NF-kB/SEAPorter cells were plated in 96-well plate at 5 x 10^4 cells per well for 16 h. Cells were pre-incubated with TNF alpha antibody at 10 and 20 ug/ml for 1 hr, then treated with 10 ng/ml TNF alpha or 10 ng/ml PMA. After 24h, the secreted alkaline phosphatase (SEAP) was analyzed using the SEAPorterAssay Kit . Image using the Azide Free format of this antibody.](http://images.novusbio.com/fullsize/TNF-alpha-Antibody-6N1E7-Inhibition-Activity-NBP2-27223-img0006.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0008.jpg "Western Blot: IKK beta Antibody [NBP1-32258] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:500.")
![Immunocytochemistry/Immunofluorescence: IKK beta Antibody [NBP1-32258] - analysis of A431, using IKK beta antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32258-img0002.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody [NBP1-32258] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NBP1-32258-img0003.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - IKK beta antibody detects IKK beta protein by western blot analysis. A. 30 ug 293T whole cell extract. B. 30 ug whole cell extract of DDDDK-human IKBKB-transfected 293T cells. 7.5 % SDS-PAGE. IKK beta antibody dilution: 1:5000](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0006.jpg)
![Western Blot: IKK beta Antibody [NBP1-32258] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IKK beta antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NBP1-32258-img0007.jpg)