| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52662-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-58867SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54394-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-75977SS | |||
| Azide and BSA Free | NBP2-80764 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-43982 | |||
| BSA Free | NBP2-61415-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80699-25ul | |||
![Western Blot: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - analysis of Integrin alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: MCF-7 cell lysateLane 2: A431 cell lysate](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Western-Blot-NBP2-67691-img0009.jpg "Western Blot: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - analysis of Integrin alpha 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: MCF-7 cell lysateLane 2: A431 cell lysate")
![Immunocytochemistry/Immunofluorescence: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Staining ITGA2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunocytochemistry-Immunofluorescence-NBP2-67691-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded mouse stomach tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0008.jpg)
![Flow Cytometry: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of A431 cells with ITGA2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Flow-Cytometry-NBP2-67691-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Staining ITGA2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunocytochemistry-Immunofluorescence-NBP2-67691-img0002.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0004.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded human colon cancer tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0005.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded human kidney tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0006.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded mouse colon tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Integrin-alpha-2-CD49b-Antibody-SN0752-Immunohistochemistry-Paraffin-NBP2-67691-img0007.jpg)
![Immunohistochemistry-Paraffin: CCR8 Antibody [NB100-709] - Section of mouse spleen.](http://images.novusbio.com/fullsize/CCR8-Antibody-Immunohistochemistry-Paraffin-NB100-709-img0002.jpg "Immunohistochemistry-Paraffin: CCR8 Antibody [NB100-709] - Section of mouse spleen.")
![Flow Cytometry: CD19 Antibody (HD37) - Chimeric [NBP2-52662] - Flow-cytometry using anti-CD19 antibody HD37. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of HD37 (NBP2-52662, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD19-Antibody-HD37-Chimeric-Flow-Cytometry-NBP2-52662-img0001.jpg "Flow Cytometry: CD19 Antibody (HD37) - Chimeric [NBP2-52662] - Flow-cytometry using anti-CD19 antibody HD37. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of HD37 (NBP2-52662, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Western Blot: Coronin-1a Antibody [NB110-58867] - Analysis of tissue lysates using Coronin-1a Antibody, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the Coronin-1a protein.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Western-Blot-NB110-58867-img0003.jpg "Western Blot: Coronin-1a Antibody [NB110-58867] - Analysis of tissue lysates using Coronin-1a Antibody, dilution 1:5,000: [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55kDa corresponds to the Coronin-1a protein.")
![Immunocytochemistry/Immunofluorescence: Coronin-1a Antibody [NB110-58867] - Analysis of cortical neuron-glial cell culture from E20 rat stained with Coronin-1a Antibody, dilution 1:1,000 (Red), and costained with mouse mAb to GFAP, dilution 1:1,000 (Green). Hoechst staining of nuclear DNA (Blue). The Coronin-1a Antibody labels protein expressed in the cytoplasm of microglia cells, while GFAP antibody stains intermediate filaments in astrocytic cells.](http://images.novusbio.com/fullsize/Coronin-1a-Antibody-Immunocytochemistry-Immunofluorescence-NB110-58867-img0004.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-IFNG-466-Immunohistochemistry-Paraffin-NBP2-53332-img0003.jpg "Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)")
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-IFNG-466-Immunohistochemistry-Paraffin-NBP2-53332-img0001.jpg)
![Immunohistochemistry-Paraffin: IFN-gamma Antibody (IFNG/466) [NBP2-53332] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Monoclonal Antibody (IFNG/466)](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-IFNG-466-Immunohistochemistry-Paraffin-NBP2-53332-img0002.jpg)
![Immunohistochemistry: CD45 Antibody (5C16) [NB110-93609] - CD45 antibody (5C16) [NB110-93609] immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-NB110-93609-img0001.jpg)
![Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609] - Correlation of MAP17 expression with inflammation in human samples of Crohn's disease & psoriasisMAP17, CD45 & CD68 staining of histological slides of human samples of psoriasis & Crohn's disease. MAP17 staining in both inflammatory diseases showed high expression levels, correlated with the inflammatory markers CD45 & CD68. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29228712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-93609_rat-monoclonal-cd45-antibody-5c16-310202415171311.jpg)
![SDS-Page: Recombinant Human Perforin Protein [H00005551-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Perforin-Protein-SDS-Page-H00005551-P01-img0002.jpg "SDS-Page: Recombinant Human Perforin Protein [H00005551-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Western-Blot-NBP2-75977-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Total protein from Human U87 cells treated with or without CoCl2 and under Normoxic and Hypoxic conditions was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-HIF-1 alpha in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HIF-1 alpha knockout HeLa cell line (KO) untreated (-) or treated (+) with 1 mM DFO for overnight. PVDF membrane was probed with 2 ug/mL of Rabbit Anti-Human HIF-1 alpha Monoclonal Antibody (NBP2-75977) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HIF-1 alpha at an approximatel molecular weight of 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control.This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Knockdown-Validated-NBP2-75977-img0003.jpg)
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Immunofluorescent staining of HIF-1 alpha using catalog number NBP2-75977 (clone 2443B). Staining was observed in Hela cells treated with DFO but not in untreated Hela cells.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Immunocytochemistry-Immunofluorescence-NBP2-75977-img0001.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Lane view shows a specific band for HIF-1 alpha in HepG2 +/- DFO cell lysate using 20ug/ml. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 116 KDa. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0004.jpg)
![Simple Western: HIF-1 alpha Antibody (2443B) [NBP2-75977] - Simple Western lane view shows lysates of MCF +/- DMOG, BioSpherix MSCs in hypoxic conditions, and BioSpherix MSCs in normoxic conditions loaded at 0.5 mg/ml. A band was detected for HIF-1 alpha at approximately 116 kDa (as indicated) using NBP2-75977 (1:100 dilution) followed by Anti-Rabbit Secondary Antibody (042-206, ProteinSimple). This experiment was conducted under standard assay conditions, and using the 12-230 kDa separation module (SW-W004). Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody. Image from an internal validation.](http://images.novusbio.com/fullsize/HIF-1-alpha-Antibody-2443B-Simple-Western-NBP2-75977-img0005.jpg)
![Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.](http://images.novusbio.com/fullsize/CTGF-CCN2-Antibody-CL5339-Western-Blot-NBP2-61415-img0007.jpg "Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.")
![Immunohistochemistry-Paraffin: EOMES Antibody [NBP1-80699] - Staining of human lymph node tissues shows moderate nuclear positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/EOMES-Antibody-Immunohistochemistry-Paraffin-NBP1-80699-img0008.jpg "Immunohistochemistry-Paraffin: EOMES Antibody [NBP1-80699] - Staining of human lymph node tissues shows moderate nuclear positivity in non-germinal center cells.")
![Immunohistochemistry-Paraffin: EOMES Antibody [NBP1-80699] - Staining of human cerebral cortex shows moderate nuclear positivity in neurons.](http://images.novusbio.com/fullsize/EOMES-Antibody-Immunohistochemistry-Paraffin-NBP1-80699-img0007.jpg)
.png "CD4 was detected in immersion fixed paraffin-embedded sections of mouse spleen using Rabbit Anti-Mouse CD4, Monoclonal Antibody (Catalog# NBP3-14741) at 1:250 dilution at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.")
![Western Blot: CD4 Antibody (BLR167J) [NBP3-14741] - Whole cell lysate (10 ug) from NIH 3T3, BW5147.3, mouseThymus, 70Z/3, and EL4 cells prepared using NETN lysisbuffer. Antibody: Rabbit anti-CD4 recombinant monoclonalantibody [BLR167J] used at 1:1000.Secondary: HRP-conjugated goat anti-rabbit IgGChemiluminescence with an exposure timeof 30 seconds. Lower Panel: Rabbit anti-Actin recombinantmonoclonal antibody [BLR057F].](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Western-Blot-NBP3-14741-img0003.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse lung, : Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0006.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse spleen, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0004.jpg)
![Immunohistochemistry-Paraffin: CD4 Antibody (BLR167J) [NBP3-14741] - Mouse gut, Rabbit anti-CD4 recombinant monoclonal antibody [BLR167J]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD4-Antibody-BLR167J-Immunohistochemistry-Paraffin-NBP3-14741-img0005.jpg)
![Immunoprecipitation: CD4 Antibody [NBP3-14741] - s: Whole cell lysate (200 ugper IP reaction; 10% of IP loaded) from mouse thymus.Antibodies: Rabbit anti-CD4 recombinant monoclonalantibody used for IP at 6 ul/200ug lysate. CD4 was also immunoprecipitated by anotherantibody against a different epitope of CD4 For blotting immunoprecipitated CD4, was used at 1:1000. Chemiluminescence withan exposure time of 30 seconds.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunoprecipitation-NBP3-14741-img0001.jpg)
![ELISA: Rat IFN-beta ELISA Kit (Colorimetric) [NBP3-06753] - Samples were spiked with high concentrations of Rat IFN-beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06753_rat-ifn-beta-elisa-kit-colorimetric-13220241613093.jpg "ELISA: Rat IFN-beta ELISA Kit (Colorimetric) [NBP3-06753] - Samples were spiked with high concentrations of Rat IFN-beta and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat IFN-beta ELISA Kit (Colorimetric) [NBP3-06753] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06753_rat-ifn-beta-elisa-kit-colorimetric-4320248595118.jpg)