| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89884-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50435-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11983-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75900SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12009-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52658-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12048-0.025mg | |||
![Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/Clusterin-APOJ-Antibody-013-Flow-Cytometry-NBP2-89884-img0002.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: Clusterin/APOJ Antibody (013) [NBP2-89884] - Analysis of Human CLU on A549 cells. Cells were treated, stained with purified anti-Human CLU, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (013) [NBP2-89884] - Staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-89884 antibody (1:60) at 37c 1 hour. Then cells were stained with the Alexa Fluor(R) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm.](http://images.novusbio.com/fullsize/nbp2-89884_rabbit-monoclonal-clusterin-apoj-antibody-013-235202514371330.png)
![Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-278202416285213.jpg "Western Blot: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Western blot analysis of LAG-3 on different lysates with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/2,000 dilution. Lane 1: NIH/3T3 transfected with LAG3 expression vector, whole cell lysate Lane 2: NIH/3T3 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 57.4 kDa Observed band size: 55/70 kDa (Glycosylation) Exposure time: 1 minute 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32513) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: LAG-3 Antibody (PSH0-42) [NBP3-32513] - Immunocytochemistry analysis of NIH/3T3 cells transfected with or without LAG3 labeling LAG-3 with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution. Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-LAG-3 antibody (NBP3-32513) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32513_rabbit-lag-3-mab-psh0-42-2920241192322.jpg)
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0002.jpg "Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).")
![Flow (Intracellular): Myeloperoxidase/MPO Antibody (MPO421-8B2) [NBP2-41406] - Intracellular staining pattern of human peripheral whole blood stained using anti-human Myeloperoxidase (MPO421-8B2) purified antibody (concentration in sample 1 ug/ml).](http://images.novusbio.com/fullsize/Myeloperoxidase-MPO-Antibody-MPO421-8B2-Flow-Intracellular-NBP2-41406-img0001.jpg)
![Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.](http://images.novusbio.com/fullsize/Complement-C3d-Antibody-E28-P-Immunohistochemistry-Paraffin-NBP1-79074-img0002.jpg "Immunohistochemistry-Paraffin: Complement C3d Antibody (E28-P) [NBP1-79074] - Formalin-fixed and paraffin-embedded human skin biopsy (4 um) from lesion of the early pemphigus vulgaris (without blister formation), stained with anti-C3d complement antibody shows strong positive intraepidermal intercellular immunostaining.")
![Immunohistochemistry-Paraffin: IkB-beta Antibody (1B5) [H00004793-M01] - Analysis of monoclonal antibody to NFKBIB on formalin-fixed paraffin-embedded human lung. Antibody concentration 5 ug/ml.](http://images.novusbio.com/fullsize/IkB-beta-Antibody-1B5-Immunohistochemistry-Paraffin-H00004793-M01-img0004.jpg "Immunohistochemistry-Paraffin: IkB-beta Antibody (1B5) [H00004793-M01] - Analysis of monoclonal antibody to NFKBIB on formalin-fixed paraffin-embedded human lung. Antibody concentration 5 ug/ml.")
![Sandwich ELISA: IkB-beta Antibody (1B5) [H00004793-M01] - Detection limit for recombinant GST tagged NFKBIB is approximately 1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/IkB-beta-Antibody-1B5-Sandwich-ELISA-H00004793-M01-img0005.jpg)
![Western Blot: IFN-gamma Antibody (N3-P3A5A10) [NBP2-50435]](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-N3-P3A5A10-Western-Blot-NBP2-50435-img0002.jpg "Western Blot: IFN-gamma Antibody (N3-P3A5A10) [NBP2-50435]")
![Immunohistochemistry: IFN-gamma Antibody (N3-P3A5/A10) [NBP2-50435] - Staining of a whole mount Zebra fish embryo.](http://images.novusbio.com/fullsize/IFN-gamma-Antibody-N3-P3A5A10-Immunofluorescence-NBP2-50435-img0001.jpg)
![Immunohistochemistry: CD4 Antibody (YNB46.1.8 (Campath-9H)) [NBP2-75900] - Anti-CD4 staining of formaldehyde fixed paraffin embedded rabbit spleen tissue, at 40x magnification. The rat IgG1-chimeric version of YNB46.1.8 (NBP2-75900) was used to stain samples at a concentration of 5 ug/ml.](http://images.novusbio.com/fullsize/CD4-Antibody-YNB46-1-8-Campath-9H-Immunohistochemistry-NBP2-75900-img0001.jpg "Immunohistochemistry: CD4 Antibody (YNB46.1.8 (Campath-9H)) [NBP2-75900] - Anti-CD4 staining of formaldehyde fixed paraffin embedded rabbit spleen tissue, at 40x magnification. The rat IgG1-chimeric version of YNB46.1.8 (NBP2-75900) was used to stain samples at a concentration of 5 ug/ml.")
![Western Blot: CD4 Antibody (YNB46.1.8 (Campath-9H)) - Azide and BSA Free [NBP2-75900] - Mouse thymus (lane 1) and mouse spleen (lane 2) were resolved on a 10% SDS PAGE gel and blots probed with NBP2-75900 at 1.5 ug/ml before being detected by a secondary antibody. The expected band size (51 kDa) is seen along with bands corresponding to dimers.](http://images.novusbio.com/fullsize/nbp2-75900_rat-monoclonal-cd4-antibody-ynb46-1-8-campath-9h-7420252211305.png)
![Flow Cytometry: IL6R Antibody (D7715A7) - Chimeric [NBP3-12009] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (NBP3-12009, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/IL6R-Antibody-D7715A7-Chimeric-Flow-Cytometry-NBP3-12009-img0002.jpg "Flow Cytometry: IL6R Antibody (D7715A7) - Chimeric [NBP3-12009] - Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (NBP3-12009, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: IL6R Antibody (D7715A7) - Chimeric - Azide and BSA Free [NBP3-12009] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix(TM) cover-slips and stained with NBP3-12009 at 10 ug/ml followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP3-12009, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12009_rabbit-monoclonal-il6r-antibody-d7715a7-chimeric-74202522141243.png)
![Flow Cytometry: CD4 Antibody (YNB46.1.8 (Campath-9H)) - Chimeric [NBP2-52658] - Flow-cytometry using anti-CD4 antibody YNB46.1.8 (Campath-9H) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of YNB46.1.8/Campath-9H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD4-Antibody-YNB46-1-8-Campath-9H-Chimeric-Flow-Cytometry-NBP2-52658-img0002.jpg "Flow Cytometry: CD4 Antibody (YNB46.1.8 (Campath-9H)) - Chimeric [NBP2-52658] - Flow-cytometry using anti-CD4 antibody YNB46.1.8 (Campath-9H) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of YNB46.1.8/Campath-9H (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Immunocytochemistry /Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048] - Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with NBP3-12048 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12048, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12048_rabbit-monoclonal-marco-antibody-plk1-chimeric-74202522113028.png "Immunocytochemistry /Immunofluorescence: MARCO Antibody (PLK1) - Chimeric - Azide and BSA Free [NBP3-12048] - Immunofluorescence analysis of paraformaldehyde fixed Human peripheral blood leukocytes on Shi-fix(TM) coverslips stained with NBP3-12048 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12048, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")