| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-72941 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49050-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37303-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48701-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15135-25ul | |||
| Azide and BSA Free | NBP3-44364 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12068-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11515 | |||
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0015.jpg "Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - A. 50 ug rat kidney lysate/extract 7.5 % SDS-PAGECD10 antibody [N2C1], Internal dilution: 1:500](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0007.jpg)
![Western Blot: Neprilysin/CD10 Antibody [NBP2-15771] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD10 antibody [N2C1], Internal diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Western-Blot-NBP2-15771-img0013.jpg)
![Western Blot: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of Neprilysin/CD10 on different lysates with Rabbit anti-Neprilysin/CD10 antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate (10 ug/Lane) Lane 2: Mouse small intestine tissue lysate (20 ug/Lane) Lane 3: Mouse kidney tissue lysate (20 ug/Lane) Lane 4: Mouse placenta tissue lysate (20 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Western-Blot-NBP2-67717-img0009.jpg "Western Blot: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of Neprilysin/CD10 on different lysates with Rabbit anti-Neprilysin/CD10 antibody at 1/1,000 dilution. Lane 1: Daudi cell lysate (10 ug/Lane) Lane 2: Mouse small intestine tissue lysate (20 ug/Lane) Lane 3: Mouse kidney tissue lysate (20 ug/Lane) Lane 4: Mouse placenta tissue lysate (20 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of 293 cells labeling CD10 with Rabbit anti-Neprilysin/CD10 antibody (permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD10 antibody was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunocytochemistry-Immunofluorescence-NBP2-67717-img0010.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human small intestine tissue with Rabbit anti-Neprilysin/CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0013.jpg)
![Flow Cytometry: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of Jurkat cells with CD10 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Flow-Cytometry-NBP2-67717-img0001.jpg)
![Western Blot: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of CD10 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Western-Blot-NBP2-67717-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Staining CD10 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunocytochemistry-Immunofluorescence-NBP2-67717-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Staining CD10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunocytochemistry-Immunofluorescence-NBP2-67717-img0003.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-CD10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0004.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0005.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human tonsil tissue using anti-CD10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0006.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0007.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-Neprilysin/CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0011.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (SN75-07) [NBP2-67717] - Analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-Neprilysin/CD10 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-SN75-07-Immunohistochemistry-Paraffin-NBP2-67717-img0012.jpg)
![Western Blot: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neprilysin/CD10.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Western-Blot-NBP2-45817-img0010.jpg "Western Blot: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Neprilysin/CD10.")
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Carcinoma of Human bladder tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0009.jpg)
![Flow Cytometry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - analysis of living 293T cells transfected with MME overexpression plasmid .](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Flow-Cytometry-NBP2-45817-img0011.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human liver tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0001.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Carcinoma of Human kidney tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0002.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human prostate tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0003.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human pancreas tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0004.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Adenocarcinoma of Human ovary tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0005.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Carcinoma of Human thyroid tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0006.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human lymphoma tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0007.jpg)
![Immunohistochemistry: Neprilysin/CD10 Antibody (OTI3D11) [NBP2-45817] - Analysis of Human Kidney tissue.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-OTI3D11-Immunohistochemistry-NBP2-45817-img0008.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0006.jpg "Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP-CyTM5.5.")
![Flow Cytometry: CD10 Antibody (MEM-78) [NB500-372]-Fig. 1. Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD10 (MEM-78) PE. Total viable cells were used for analysis.](http://images.novusbio.com/fullsize/p_444_1.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0001.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) APC-CyTM7.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0002.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-CyTM5.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0003.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PE-DyLigt(R) 594.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0004.jpg)
![Flow (Cell Surface): Neprilysin/CD10 Antibody (MEM-78) [NB500-372] - Surface staining of human peripheral blood with anti-human CD10 (MEM-78) PerCP.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-MEM-78-Flow-Cell-Surface-NB500-372-img0005.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-49050] - Staining of human prostate.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-49050-img0007.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-49050] - Staining of human prostate.")
![Immunohistochemistry: Neprilysin/CD10 Antibody [NBP2-49050] - Staining of human duodenum shows strong positivity brush border.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-NBP2-49050-img0001.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-49050] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-49050-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-49050] - Staining in human duodenum and cerebral cortex tissues using anti-MME antibody. Corresponding MME RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-49050-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-49050] - Staining of human cerebral cortex, kidney, prostate and small intestine using Anti-MME antibody NBP2-49050 (A) shows similar protein distribution across tissues to independent antibody NBP2-48701 (B).](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-49050-img0004.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-49050] - Staining of human small intestine.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-49050-img0005.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-49050] - Staining of human kidney.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-49050-img0006.jpg)
![Western Blot: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Western blot analysis using CD10 mouse mAb against LNCAP cell lysate.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-7D4B1-Western-Blot-NBP2-37303-img0004.jpg "Western Blot: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Western blot analysis using CD10 mouse mAb against LNCAP cell lysate.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Analysis of paraffin-embedded cervical cancer tissues using CD10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-7D4B1-Immunohistochemistry-Paraffin-NBP2-37303-img0007.jpg)
![Western Blot: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Western blot analysis using CD10 mAb against human CD10 (AA: 52-246) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-7D4B1-Western-Blot-NBP2-37303-img0002.jpg)
![Western Blot: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 52-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-7D4B1-Western-Blot-NBP2-37303-img0003.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Analysis of paraffin-embedded prostate cancer tissues using CD10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-7D4B1-Immunohistochemistry-Paraffin-NBP2-37303-img0008.jpg)
![ELISA: Neprilysin/CD10 Antibody (7D4B1) [NBP2-37303] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-7D4B1-ELISA-NBP2-37303-img0001.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human prostate.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0005.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human prostate.")
![Immunohistochemistry: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human small intestine shows membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-NBP2-48701-img0001.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0002.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining in human small intestine and cerebral cortex tissues using anti-MME antibody. Corresponding MME RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0003.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human cerebral cortex, kidney, prostate and small intestine using Anti-MME antibody NBP2-48701 (A) shows similar protein distribution across tissues to independent antibody NBP2-49050 (B).](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0004.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody [NBP2-48701] - Staining of human kidney.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-Immunohistochemistry-Paraffin-NBP2-48701-img0006.jpg)
![Western Blot: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Western blot analysis of Neprilysin/CD10 on different lysates with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: Ramos cell lysate (20 ug/Lane) Lane 3: LNCaP cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) (negative) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-27820241631626.jpg "Western Blot: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Western blot analysis of Neprilysin/CD10 on different lysates with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: Ramos cell lysate (20 ug/Lane) Lane 3: LNCaP cell lysate (20 ug/Lane) Lane 4: HT-29 cell lysate (20 ug/Lane) (negative) Lane 5: Mouse kidney tissue lysate (40 ug/Lane) Predicted band size: 86 kDa Observed band size: 100 kDa Exposure time: 5 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32118) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32118) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-298202414161875.jpg)
![Immunocytochemistry/ Immunofluorescence: Neprilysin/CD10 Antibody (A1G3-R) [NBP3-32118] - Immunocytochemistry analysis of Raji cells labeling Neprilysin/CD10 with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Neprilysin/CD10 antibody (NBP3-32118) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32118_mouse-neprilysin-cd10-mab-a1g3-r-29202410484874.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human small intestine shows strong apical membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0004.jpg "Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human small intestine shows strong apical membranous positivity in glandular cells.")
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human kidney shows strong apical membranous positivity in cells in tubules and cells in glomeruli.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0001.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human pancreas shows very weak positivity in exocrine glandular cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0002.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Staining of human skin shows very weak positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0003.jpg)
![Immunohistochemistry-Paraffin: Neprilysin/CD10 Antibody (CL12448) [NBP3-15135] - Analysis in human small intestine and pancreas tissues using NBP3-15135 antibody. Corresponding MME RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-CL12448-Immunohistochemistry-Paraffin-NBP3-15135-img0005.jpg)
![Western Blot: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 52-246)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-5E9A5-Western-Blot-NBP2-37301-img0003.jpg "Western Blot: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Western blot analysis using CD10 mAb against HEK293 (1) and CD10 (AA: 52-246)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunohistochemistry: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CD10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-5E9A5-Immunohistochemistry-NBP2-37301-img0004.jpg)
![Western Blot: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Western blot analysis using CD10 mAb against human CD10 (AA: 52-246) recombinant protein. (Expected MW is 41.4 kDa)](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-5E9A5-Western-Blot-NBP2-37301-img0002.jpg)
![ELISA: Neprilysin/CD10 Antibody (5E9A5) [NBP2-37301] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-5E9A5-ELISA-NBP2-37301-img0001.jpg)
![Western Blot: Neprilysin/CD10 Antibody (FR4D11) - Chimeric [NBP3-12068] - Daudi cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (NBP3-12068) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-FR4D11-Chimeric-Western-Blot-NBP3-12068-img0001.jpg "Western Blot: Neprilysin/CD10 Antibody (FR4D11) - Chimeric [NBP3-12068] - Daudi cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of FR4D11 (NBP3-12068) at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) - Chimeric - Azide and BSA Free [NBP3-12068] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with NBP3-12068 at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12068, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-12068_rabbit-monoclonal-neprilysin-cd10-antibody-fr4d11-chimeric-74202522141215.png)
![Flow Cytometry: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868] - Flow Cytometric Analysis of Ramos cells using Neprilysin/CD10 Antibody (FR4D11) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-FR4D11-Flow-Cytometry-NBP2-44868-img0002.jpg "Flow Cytometry: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868] - Flow Cytometric Analysis of Ramos cells using Neprilysin/CD10 Antibody (FR4D11) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).")
![Immunofluorescence: Neprilysin/CD10 Antibody (FR4D11) [NBP2-44868] - ICC/IF staining of Ramos cells using followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/Neprilysin-CD10-Antibody-FR4D11-Immunofluorescence-NBP2-44868-img0003.jpg)
