| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-09017-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44715 | |||
| Azide and BSA Free | NBP2-34519-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-81332-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79885 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-38265-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84882-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50163-0.025ml | |||
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/MHC-Class-I-Antibody-R1-21-2-Chimeric-Western-Blot-NBP3-09017-img0003.jpg "Western Blot: MHC Class I Antibody (R1-21.2) - Chimeric [NBP3-09017] - Mouse thymus tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with NBP3-09017 at 1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry /Immunofluorescence: MHC Class I Antibody (R1-21.2) - Chimeric - Azide and BSA Free [NBP3-09017] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized and stained with NBP3-09017 at 10 ug/ml followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-09017, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (NBP2-52638) followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-09017_rabbit-monoclonal-mhc-class-i-antibody-r1-21-2-chimeric-74202522265217.png)
![Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-33627] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:5000.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Western-Blot-NBP1-33627-img0007.jpg "Western Blot: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-33627] - Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE; antibody diluted at 1:5000.")
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-33627] - GSTP1 antibody detects GSTP1 protein at cytosol on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. GSTP1 antibody dilution: 1:500.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP1-33627-img0009.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-33627] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP1-33627-img0006.jpg)
![GSTP1 antibody [N1N2], N-term detects GSTP1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GSTP1 protein stained by GSTP1 antibody [N1N2], N-term (NBP1-33627) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33627_rabbit-polyclonal-glutathione-s-transferase-pi-1-gstp1-antibody-immunocytochemistry-immunofluorescence-261120251827234.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5 + 123A8) [NBP2-34280] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD56 Monoclonal Antibody (123C3.D5 + 123A8)](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-+-123A8-Immunohistochemistry-Paraffin-NBP2-34280-img0003.jpg "Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5 + 123A8) [NBP2-34280] - Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with CD56 Monoclonal Antibody (123C3.D5 + 123A8)")
![Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5 + 123A8) [NBP2-34280] - Flow cytometry of lymphocyte gated PBMCs unstained (gray) or stained with CF568-labeled NCAM-1/CD56 antibody (123C3.D5 + 123A8) (orange).](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-+-123A8-Flow-Cytometry-NBP2-34280-img0004.jpg)
![Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5 + 123A8) [NBP2-34280] - Analysis using the Azide and BSA Free version of NBP2-34280. Staining of human colon ganglion stained with CD56 MAb (123C3.D5+123A8).](http://images.novusbio.com/fullsize/NCAM-1-CD56-Antibody-123C3-D5-+-123A8-Immunohistochemistry-Paraffin-NBP2-34280-img0001.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Western-Blot-H00001108-M01-img0009.jpg "Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Immunohistochemistry-Paraffin-H00001108-M01-img0006.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - CHD4 monoclonal antibody (M01), clone 4H4 Analysis of CHD4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Western-Blot-H00001108-M01-img0008.jpg)
![Sandwich ELISA: CHD4 Antibody (4H4) [H00001108-M01] - Detection limit for recombinant GST tagged CHD4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Sandwich-ELISA-H00001108-M01-img0007.jpg)
![Western Blot: Serpin G1/C1 Inhibitor Antibody (3F4-1D9) [H00000710-M01] - Analysis of SERPING1 expression in transfected 293T cell line by SERPING1 monoclonal antibody (M01), clone 3F4-1D9.Lane 1: SERPING1 transfected lysate (Predicted MW: 55.2 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-3F4-1D9-Western-Blot-H00000710-M01-img0010.jpg "Western Blot: Serpin G1/C1 Inhibitor Antibody (3F4-1D9) [H00000710-M01] - Analysis of SERPING1 expression in transfected 293T cell line by SERPING1 monoclonal antibody (M01), clone 3F4-1D9.Lane 1: SERPING1 transfected lysate (Predicted MW: 55.2 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: Serpin G1/C1 Inhibitor Antibody (3F4-1D9) [H00000710-M01] - Analysis of monoclonal antibody to SERPING1 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1 ug/ml.](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-3F4-1D9-Immunohistochemistry-Paraffin-H00000710-M01-img0006.jpg)
![Western Blot: Serpin G1/C1 Inhibitor Antibody (3F4-1D9) [H00000710-M01] - SERPING1 monoclonal antibody (M01), clone 3F4-1D9. Analysis of SERPING1 expression in human liver.](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-3F4-1D9-Western-Blot-H00000710-M01-img0009.jpg)
![Immunoprecipitation: Serpin G1/C1 Inhibitor Antibody (3F4-1D9) [H00000710-M01] - Analysis of SERPING1 transfected lysate using anti-SERPING1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SERPING1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-3F4-1D9-Immunoprecipitation-H00000710-M01-img0007.jpg)
![Sandwich ELISA: Serpin G1/C1 Inhibitor Antibody (3F4-1D9) [H00000710-M01] - Detection limit for recombinant GST tagged SERPING1 is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Serpin-G1-C1-Inhibitor-Antibody-3F4-1D9-Sandwich-ELISA-H00000710-M01-img0008.jpg)
![Western Blot: RPL3 Antibody [NBP1-81332] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp](http://images.novusbio.com/fullsize/RPL3-Antibody-Western-Blot-NBP1-81332-img0005.jpg "Western Blot: RPL3 Antibody [NBP1-81332] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp")
![Immunocytochemistry/Immunofluorescence: RPL3 Antibody [NBP1-81332] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/RPL3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81332-img0006.jpg)
![Immunohistochemistry-Paraffin: RPL3 Antibody [NBP1-81332] - Staining of human cerebral cortex shows strong cytoplasmic and nuclear(nucleolar) positivity in neuronal cells.](http://images.novusbio.com/fullsize/RPL3-Antibody-Immunohistochemistry-Paraffin-NBP1-81332-img0002.jpg)
![Western Blot: BOB1 Antibody (BOB1/2421) [NBP2-79750] - Western Blot Analysis of Raji cell lysate using BOB1 Antibody (BOB1/2421).](http://images.novusbio.com/fullsize/BOB1-Antibody-BOB1-2421-Western-Blot-NBP2-79750-img0004.jpg "Western Blot: BOB1 Antibody (BOB1/2421) [NBP2-79750] - Western Blot Analysis of Raji cell lysate using BOB1 Antibody (BOB1/2421).")
![Immunohistochemistry-Paraffin: BOB1 Antibody (BOB1/2421) [NBP2-79750] - Formalin-fixed, paraffin-embedded human Spleen stained with BOB1 Antibody (BOB1/2421).](http://images.novusbio.com/fullsize/BOB1-Antibody-BOB1-2421-Immunohistochemistry-Paraffin-NBP2-79750-img0002.jpg)
![Flow Cytometry: BOB1 Antibody (BOB1/2421) [NBP2-79750] - Flow Cytometric Analysis of Human Raji cells using BOB1 Antibody (BOB1/2421)followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/BOB1-Antibody-BOB1-2421-Flow-Cytometry-NBP2-79750-img0001.jpg)
![Protein Array: BOB1 Antibody (BOB1/2421) [NBP2-79750] - Analysis of Protein Array containing more than 19,000 full-length human proteins using BOB1 Antibody (BOB1/2421). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/BOB1-Antibody-BOB1-2421-Protein-Array-NBP2-79750-img0003.jpg)
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-2120249413389.jpg "Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-2120249415936.jpg)
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-212024941421.jpg)
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-2120249374988.jpg)
![Western Blot: RNase L Antibody (HL2341) - Azide and BSA Free [NBP3-25640] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RNase L antibody [HL2341] (NBP3-25640) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25640_rabbit-rnase-l-mab-hl2341-azide-and-bsa-free-2120249432829.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody [NBP2-38265] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/TrkA-Antibody-Immunohistochemistry-Paraffin-NBP2-38265-img0005.jpg "Immunohistochemistry-Paraffin: TrkA Antibody [NBP2-38265] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.")
![Immunohistochemistry-Paraffin: TrkA Antibody [NBP2-38265] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neuronal cells.](http://images.novusbio.com/fullsize/TrkA-Antibody-Immunohistochemistry-Paraffin-NBP2-38265-img0001.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody [NBP2-38265] - Staining of human adrenal gland shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/TrkA-Antibody-Immunohistochemistry-Paraffin-NBP2-38265-img0002.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody [NBP2-38265] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TrkA-Antibody-Immunohistochemistry-Paraffin-NBP2-38265-img0003.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody [NBP2-38265] - Staining of human liver shows no positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/TrkA-Antibody-Immunohistochemistry-Paraffin-NBP2-38265-img0004.jpg)
![Immunohistochemistry-Paraffin: TrkA Antibody [NBP2-38265] - Analysis in human adrenal gland and prostate tissues using NBP2-38265 antibody. Corresponding TrkA RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/TrkA-Antibody-Immunohistochemistry-Paraffin-NBP2-38265-img0006.jpg)
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84882] - Staining of human testis.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84882-img0008.jpg "Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84882] - Staining of human testis.")
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84882] - Staining of human placenta shows nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84882-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84882] - Staining of human colon, kidney, testis and vagina using Anti-ZNF521 antibody NBP1-84882 (A) shows similar protein distribution across tissues to independent antibody NBP1-84883 (B).](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84882-img0005.jpg)
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84882] - Staining of human colon.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84882-img0006.jpg)
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84882] - Staining of human kidney.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84882-img0007.jpg)
![Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84882] - Staining of human vagina.](http://images.novusbio.com/fullsize/ZNF521-Antibody-Immunohistochemistry-Paraffin-NBP1-84882-img0009.jpg)
![Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CHD4-Antibody-CHD4-3F2-4-Flow-Cytometry-NBP2-50163-img0001.jpg "Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")