| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81113-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20109-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52657-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-27121-0.025ml | |||
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0015.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.")
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0014.jpg)
![Western Blot: HLA Class I Antibody [NBP2-75928] - Western blot analysis of HLA Class I on Hela cell lysate using anti-HLA Class I antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/HLA-Class-I-Antibody-Western-Blot-NBP2-75928-img0008.jpg "Western Blot: HLA Class I Antibody [NBP2-75928] - Western blot analysis of HLA Class I on Hela cell lysate using anti-HLA Class I antibody at 1/1,000 dilution.")
![Flow Cytometry: HLA Class I Antibody [NBP2-75928] - analysis of Jurkat cells with HLA Class 1 ABC antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/HLA-Class-I-Antibody-Flow-Cytometry-NBP2-75928-img0007.jpg)
![Immunohistochemistry-Paraffin: HLA Class I Antibody [NBP2-75928] - analysis of paraffin-embedded human colon cancer tissue using anti-HLA Class 1 ABC antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/HLA-Class-I-Antibody-Immunohistochemistry-Paraffin-NBP2-75928-img0003.jpg)
![Western Blot: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Western blot analysis of TRAILR3/TNFRSF10C on different lysates with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27 kDa Observed band size: 27/70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32257) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-27820241529552.jpg "Western Blot: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Western blot analysis of TRAILR3/TNFRSF10C on different lysates with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/1,000 dilution. Lane 1: JAR cell lysate Lane 2: Jurkat cell lysate Lane 3: A549 cell lysate Lane 4: HeLa cell lysate Lane 5: Raji cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 27 kDa Observed band size: 27/70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32257) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Immunocytochemistry analysis of Jurkat cells labeling TRAILR3/TNFRSF10C with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TRAILR3/TNFRSF10C antibody (NBP3-32257) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-2920241114817.jpg)
![Flow Cytometry: TRAILR3/TNFRSF10C Antibody (JE58-51) [NBP3-32257] - Flow cytometric analysis of Jurkat cells labeling TRAILR3/TNFRSF10C. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32257, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32257_rabbit-trailr3-tnfrsf10c-mab-je58-51-29202416372111.jpg)
![Western Blot: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Western blot analysis of CD43/Sialophorin on different lysates with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32154) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-278202415212446.jpg "Western Blot: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Western blot analysis of CD43/Sialophorin on different lysates with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32154) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32154) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-298202414161888.jpg)
![Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunocytochemistry analysis of U-937 (positive) and HT-29 (negative) labeling CD43/Sialophorin with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-2920241114826.jpg)
![Western Blot: CD74 Antibody (PDM0-22) [NBP3-32167] - Western blot analysis of CD74 on different lysates with Mouse anti-CD74 antibody (NBP3-32167) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32167) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32167_mouse-cd74-mab-pdm0-22-278202415295513.jpg "Western Blot: CD74 Antibody (PDM0-22) [NBP3-32167] - Western blot analysis of CD74 on different lysates with Mouse anti-CD74 antibody (NBP3-32167) at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32167) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:150,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD74 Antibody (PDM0-22) [NBP3-32167] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Mouse anti-CD74 antibody (NBP3-32167) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32167) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32167_mouse-cd74-mab-pdm0-22-298202414262622.jpg)
![Immunocytochemistry/ Immunofluorescence: CD74 Antibody (PDM0-22) [NBP3-32167] - Immunocytochemistry analysis of LOVO cells labeling CD74 with Mouse anti-CD74 antibody (NBP3-32167) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-CD74 antibody (NBP3-32167) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32167_mouse-cd74-mab-pdm0-22-292024111481.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-MEM-189-Flow-Cytometry-NB500-493-img0001.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.")
![Western Blot: CD53 Antibody (JE60-38) [NBP3-32159] - Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (NBP3-32159) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32159) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-278202416331213.jpg "Western Blot: CD53 Antibody (JE60-38) [NBP3-32159] - Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (NBP3-32159) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Raji cell lysate Lane 3: Ramos cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 24 kDa Observed band size: 35-42 kDa Exposure time: 1 minute 14 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32159) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD53 Antibody (JE60-38) [NBP3-32159] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD53 antibody (NBP3-32159) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32159) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-298202414333654.jpg)
![Immunocytochemistry/ Immunofluorescence: CD53 Antibody (JE60-38) [NBP3-32159] - Immunocytochemistry analysis of Jurkat cells labeling CD53 with Rabbit anti-CD53 antibody (NBP3-32159) at 1/100 dilution. Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD53 antibody (NBP3-32159) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32159_rabbit-cd53-mab-je60-38-29202410334023.jpg)
![Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Western-Blot-NBP2-81113-img0002.jpg "Western Blot: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Western Blot using Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Human testis (A) and human ovary (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of R-125224 [NBP2-81113] at 2 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified Fas is 37.7kDa, but this protein is glycosylated at several positions leading to the observed running size.")
![Flow Cytometry: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric [NBP2-81113] - Flow-cytometry using the Fas/TNFRSF6/CD95 Antibody (R-125224) [NBP2-81113]. Jurkat cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of R-125224 (NBP2-81113, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Fas-TNFRSF6-CD95-Antibody-R-125224-Chimeric-Flow-Cytometry-NBP2-81113-img0003.jpg)
![Immunocytochemistry /Immunofluorescence: Fas/TNFRSF6/CD95 Antibody (R-125224) - Chimeric - Azide and BSA Free [NBP2-81113] - Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells permeabilized with 0.15% Triton and stained with NBP2-81113 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81113, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81113_rabbit-monoclonal-fas-tnfrsf6-cd95-antibody-r-125224-chimeric-74202522141233.png)
![Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).](http://images.novusbio.com/fullsize/nbp3-23309_rabbit-plexin-a1-pab-1992023185690.jpg "Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP3-23309] - Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin A1 rabbit polyclonal (NBP3-23309) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin A1 peptide in the presence of this anti-Plexin A1 antibody (C,F).](http://images.novusbio.com/fullsize/nbp3-23309_rabbit-plexin-a1-pab-119202316331615.jpg)
![Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924288.png "Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-43B-8 (NBP3-20109) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20109, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924248.png)
![Flow Cytometry: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-43B-8 (NBP3-20109, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-20109_rabbit-clusterin-apoj-mab-saic-43b-8-azide-and-bsa-free-2742023924262.png)
![Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric [NBP2-52657] - Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (NBP2-52657, panel B) at a concentration of 0.25 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD52-Antibody-YTH-34-5-G2b-Campath-1G-Chimeric-Flow-Cytometry-NBP2-52657-img0001.jpg "Flow Cytometry: CD52 Antibody (YTH 34.5-G2b (Campath-1G)) - Chimeric [NBP2-52657] - Flow-cytometry using the anti-CD52 research biosimilar antibody Campath-1G (YTH 34.5-G2b) Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Campath-1G / YTH 34.5-G2b (NBP2-52657, panel B) at a concentration of 0.25 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-104202492029.jpg%3Fv=1666701381 "Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).")
![Flow Cytometry: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of HepG2 cells using CARD12 (p Ser533) rat mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-10420249141939.jpg%3Fv=1666701381)
![Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-104202491625.jpg%3Fv=1666701381)
![Immunocytochemistry/Immunofluorescence: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Analysis of Hela cells using CARD12 (p Ser533) rat mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-1042024920223.jpg%3Fv=1666701381)
![ELISA: CARD12 [p Ser533] Antibody (9A6A1) [NBP3-27121] - Black line: Control Antigen (100 ng); Purple line: Antigen (10 ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](http://images.novusbio.com/fullsize/nbp3-27121_mouse-card12-p-ser533-mab-9a6a1-1042024916215.jpg%3Fv=1666701381)