| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47178SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47179SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47181SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47180SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB600-1005-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26877-25ul | |||
| Azide and BSA Free | NBP3-44280 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91212-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49516-25ul | |||
![Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-278202415212466.jpg "Western Blot: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Western blot analysis of HIF-1 alpha on different lysates with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with 250uM CoCl2 for 6 hours cell lysate Lane 3: HepG2 cell lysate Lane 4: HepG2 treated with 250uM CoCl2 for 6 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 93 kDa Observed band size: 120 kDa Exposure time: 1minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32424) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32424) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-2982024149260.jpg)
![Immunocytochemistry/ Immunofluorescence: HIF-1 alpha Antibody (JE75-33) [NBP3-32424] - Immunocytochemistry analysis of HeLa cells treated with or without 500μM CoCl2 for 24 hours labeling HIF-1 alpha with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HIF-1 alpha antibody (NBP3-32424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32424_rabbit-hif-1-alpha-mab-je75-33-29202410334071.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 9 [NBP1-47178] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-9-Antibody-exon-9-Western-Blot-NBP1-47178-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 9 [NBP1-47178] - Human recombinant HIF-1 alpha protein.")
![Western Blot: TCF-12/HTF4 Antibody [NB100-2337] - Detection of Human TCF12 on HeLa whole cell lysate using NB100-2337. TCF12 was also immunoprecipitated using rabbit anti-TCF12 antibody NB100-2338.](http://images.novusbio.com/fullsize/TCF-12-HTF4-Antibody-Western-Blot-NB100-2337-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: TCF-12/HTF4 Antibody [NB100-2337] - Detection of Human TCF12 on HeLa whole cell lysate using NB100-2337. TCF12 was also immunoprecipitated using rabbit anti-TCF12 antibody NB100-2338.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: TCF-12/HTF4 Antibody [NB100-2337] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- TCF12 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/TCF-12-HTF4-Antibody-Immunohistochemistry-NB100-2337-img0007.jpg)
![Immunohistochemistry-Paraffin: TCF-12/HTF4 Antibody [NB100-2337] - FFPE section of human colon carcinoma. Affinity purified rabbit anti-TCF12 used at a dilution of 1:250.](http://images.novusbio.com/fullsize/TCF-12-HTF4-Antibody-Immunohistochemistry-Paraffin-NB100-2337-img0003.jpg)
![Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-10-Antibody-exon-10-Western-Blot-NBP1-47179-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 10 [NBP1-47179] - Human recombinant HIF-1 alpha protein")
![Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-[exon-13]-Antibody-Western-Blot-NBP1-47181-img0002.jpg "Western Blot: HIF-1 alpha Antibody - Exon 13 [NBP1-47181] - Human recombinant HIF-1 alpha protein.")
![Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.](http://images.novusbio.com/fullsize/HIF-1-alpha-Exon-12-Antibody-exon-12-Western-Blot-NBP1-47180-img0003.jpg "Western Blot: HIF-1 alpha Antibody - Exon 12 [NBP1-47180] - Human recombinant HIF-1 alpha protein.")
![Immunohistochemistry: LYVE-1 Antibody [NB600-1005] - LYVE1 Antibody [NB600-1005] - Staining of human skin showing positivity in lymphatic vessels. Image provided by Patricia Redondo.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Immunohistochemistry-NB600-1005-img0011.jpg "Immunohistochemistry: LYVE-1 Antibody [NB600-1005] - LYVE1 Antibody [NB600-1005] - Staining of human skin showing positivity in lymphatic vessels. Image provided by Patricia Redondo.")
![Flow Cytometry: LYVE-1 Antibody [NB600-1005] - Analysis with primary human dermal microvascular endothelial cells (HDMVEC).](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Flow-Cytometry-NB600-1005-img0010.jpg)
![Western Blot: LYVE-1 Antibody [NB600-1005] - Analysis of recombinant human sLYVE-1 and mouse sLYVE-1 using an anti-human LYVE-1 polyclonal antibody directed against the extracellular domain of human LYVE-1. There is more or less no cross reactivity withmouse LYVE-1.](http://images.novusbio.com/fullsize/LYVE-1-Antibody-Western-Blot-NB600-1005-img0009.jpg)
![Western Blot: Endoglin/CD105 Antibody [NBP1-91212] - Analysis in human cell line U-138 MG.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Western-Blot-NBP1-91212-img0006.jpg "Western Blot: Endoglin/CD105 Antibody [NBP1-91212] - Analysis in human cell line U-138 MG.")
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Analysis in human placenta and pancreas tissues. Corresponding ENG RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Analysis in human placenta and pancreas tissues. Corresponding ENG RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human testis shows moderate membranous positivity in peritubular myoid cells.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0008.jpg)
![Immunohistochemistry: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human placenta shows membranous positivity in trophoblasts.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-NBP2-49516-img0001.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human kidney shows strong membranous positivity in cells in glomeruli.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0006.jpg)
![Immunohistochemistry-Paraffin: Endoglin/CD105 Antibody [NBP2-49516] - Staining of human pancreas shows low positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Endoglin-CD105-Antibody-Immunohistochemistry-Paraffin-NBP2-49516-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CXCR2/IL-8RB Antibody (547) [NBP2-43810] - CXCR2 antibody [547] detects CXCR2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR2 stained by CXCR2 antibody [547] diluted at 1:2000.Blue: Hoechst 33342 staining. Scale bar= 10um.](http://images.novusbio.com/fullsize/CXCR2-IL-8RB-Antibody-547-Immunocytochemistry-Immunofluorescence-NBP2-43810-img0002.jpg "Immunocytochemistry/Immunofluorescence: CXCR2/IL-8RB Antibody (547) [NBP2-43810] - CXCR2 antibody [547] detects CXCR2 protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR2 stained by CXCR2 antibody [547] diluted at 1:2000.Blue: Hoechst 33342 staining. Scale bar= 10um.")
![Non-transfected (-) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR2 antibody [GT547] (NBP2-43810) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-43810_mouse-monoclonal-cxcr2-il-8rb-antibody-547-western-blot-261120251974512.jpg)
![Western Blot: Fucosyltransferase 2/FUT2 Antibody [NBP2-26116] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Fucosyltransferase-2-FUT2-Antibody-Western-Blot-NBP2-26116-img0001.jpg "Western Blot: Fucosyltransferase 2/FUT2 Antibody [NBP2-26116] - Staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Immunohistochemistry-Paraffin: Fucosyltransferase 2/FUT2 Antibody [NBP2-26116] - Staining of Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Fucosyltransferase-2-FUT2-Antibody-Immunohistochemistry-Paraffin-NBP2-26116-img0002.jpg)