| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35131-1mg | |||
| Carrier-Free | NBP2-35131-100ug | |||
| Carrier-Free | NBP2-35131-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75003 | |||
| Pre-adsorbed | NBP1-75004 | |||
| Pre-adsorbed | NBP1-75005 | |||
| Pre-adsorbed | NBP1-75006 | |||
| Pre-adsorbed | NBP1-75851 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NB110-85473-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52708-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-52662-0.025mg | |||
![SDS-Page: Mouse IL-4 Protein [NBP2-35131]](http://images.novusbio.com/fullsize/Mouse-IL-4-Protein-SDS-Page-NBP2-35131-img0001.jpg "SDS-Page: Mouse IL-4 Protein [NBP2-35131]")
![Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.](http://images.novusbio.com/fullsize/E-selectin-Antibody-RM0034-9J12-Immunohistochemistry-Paraffin-NB110-85473-img0006.jpg "Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - 4% PFA fixed and paraffin embedded mouse Lung and Heart tissue section was subjected to IHC staining of mouse E-Selectin using NB110-85473.")
![Immunohistochemistry-Paraffin: E-selectin Antibody (RM0034-9J12) [NB110-85473] - The Bouins solution fixed and paraffin embedded mouse kidney section from anti-GBM model was subjected to IHC with anti mouse E-Selectin using NB110-85473.](http://images.novusbio.com/fullsize/E-selectin-Antibody-RM0034-9J12-Immunohistochemistry-Paraffin-NB110-85473-img0005.jpg)
![Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Western-Blot-NBP2-75397-img0008.jpg "Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Staining AIF-1/Iba1 in SH-SY5Y cells using (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunocytochemistry-Immunofluorescence-NBP2-75397-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Iba1 with Rabbit anti-AIF-1/Iba1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0015.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of THP-1 cells withAIF-1:Iba1 Antibody (JM36-62) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Flow-Cytometry-NBP2-75397-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human lung cancer tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded rat lung tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0007.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-AIF-1/Iba1rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0009.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded mouse brain tissue using anti-AIF-1/Iba1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0010.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0011.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0012.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0013.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0014.jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Immunocytochemistry-Immunofluorescence-NBP2-66994-img0002.jpg "Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] - Staining C3 in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.")
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Analysis of HepG2 cells with C3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Complement-C3-Antibody-JF10-30-Flow-Cytometry-NBP2-66994-img0001.jpg)
![Flow Cytometry: Complement C3 Antibody (JF10-30) [NBP2-66994] - Cells were fixed and permeabilized. Then stained with the primary antibody (1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4c for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4 c. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-flow-cytometry-1542024105623..jpg)
![Immunocytochemistry/Immunofluorescence: Complement C3 Antibody (JF10-30) [NBP2-66994] -Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-C3 antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4 c. Goat Anti-Mouse IgG H&L (iFluor™ ) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp2-66994_rabbit-monoclonal-complement-c3-antibody-jf10-30-immunocytochemistry-immunofluorescence-15420241102..jpg)
![Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Western-Blot-NBP2-67605-img0006.jpg "Western Blot: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of CD21 on Daudi celllysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0002.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: CD21 Antibody (SC0681) [NBP2-67605] - Staining CD21 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunocytochemistry-Immunofluorescence-NBP2-67605-img0001.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human gastric cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0003.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0004.jpg)
![Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0005.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: CD21 Antibody (SC0681) [NBP2-67605] - Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-61, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/CD21-Antibody-SC0681-Immunohistochemistry-Paraffin-NBP2-67605-img0007.jpg)
![Western blot analysis on different lysates with NBP2-67605 at 1/5,000 dilution. Lane 1: Raji cell lysate Lane 2: K-562 cell lysate (negative) Lysates/proteins at 20 µg/Lane. Predicted band size: 113 kDa Observed band size: 150 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67605 at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67605_-western-blot-639204586671610263.jpg)
![Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Western blot analysis of DPPIV/CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse heart tissue lysate Lane 3: human kidney tissue lysate Lane 4: Raji cell lysate Lane 5: PC-3 cell lysate](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Western-Blot-NBP2-78791-img0007.jpg "Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Western blot analysis of DPPIV/CD26 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse lung tissue lysate Lane 2: mouse heart tissue lysate Lane 3: human kidney tissue lysate Lane 4: Raji cell lysate Lane 5: PC-3 cell lysate")
![Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunocytochemistry-Immunofluorescence-NBP2-78791-img0005.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded human liver tissue using anti-CD26 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunohistochemistry-Paraffin-NBP2-78791-img0006.jpg)
![Flow Cytometry: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of Hela cells with CD26 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Flow-Cytometry-NBP2-78791-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Staining CD26 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunocytochemistry-Immunofluorescence-NBP2-78791-img0002.jpg)
![Immunohistochemistry-Paraffin: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - Analysis of paraffin-embedded rat epididymis tissue using anti-CD26 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Immunohistochemistry-Paraffin-NBP2-78791-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: DPPIV/CD26 Antibody (JM11-42) [NBP2-78791] - All lanes: Western blot analysis of CD26 with anti-CD26 antibody at 1:500 dilution.Lane 1: Wild-type Hela whole cell lysate (10 ug). Lane 2: CD26 knockout Hela whole cell lysate (10 ug).NBP2-78791 was shown to specifically react with CD26 in wild-type Hela cells. NO band was observed when CD26 knockout sample was tested. Wild-type and CD26 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/DPPIV-CD26-Antibody-JM11-42-Knockout-Validated-NBP2-78791-img0009.jpg)
![Immunohistochemistry-Paraffin: CCR8 Antibody [NB100-709] - Section of mouse spleen.](http://images.novusbio.com/fullsize/CCR8-Antibody-Immunohistochemistry-Paraffin-NB100-709-img0002.jpg "Immunohistochemistry-Paraffin: CCR8 Antibody [NB100-709] - Section of mouse spleen.")
![Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) [NBP2-52708] - Flow-cytometry using anti-CD29 antibody K20 (NBP2-52708) Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of K20 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Integrin-beta-1-CD29-Antibody-K20-NBP2-52708-img0001.jpg "Flow Cytometry: Integrin beta 1/CD29 Antibody (K20) [NBP2-52708] - Flow-cytometry using anti-CD29 antibody K20 (NBP2-52708) Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of K20 (panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: CD19 Antibody (HD37) - Chimeric [NBP2-52662] - Flow-cytometry using anti-CD19 antibody HD37. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of HD37 (NBP2-52662, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD19-Antibody-HD37-Chimeric-Flow-Cytometry-NBP2-52662-img0001.jpg "Flow Cytometry: CD19 Antibody (HD37) - Chimeric [NBP2-52662] - Flow-cytometry using anti-CD19 antibody HD37. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of HD37 (NBP2-52662, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")