| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45056 | |||
| Azide and BSA Free | NBP2-34601-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-88461-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-34130-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-73845-10mg | |||
![Immunohistochemistry-Paraffin: IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) [NBP2-34252] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM MAb (DA4-4)](http://images.novusbio.com/fullsize/IgM-Antibody-DA4-4-same-as-SA-DA4-or-HB57-Immunohistochemistry-Paraffin-NBP2-34252-img0001.jpg "Immunohistochemistry-Paraffin: IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) [NBP2-34252] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM MAb (DA4-4)")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: FMNL1 Antibody [NBP1-88461] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/FMNL1-Antibody-Immunohistochemistry-Paraffin-NBP1-88461-img0005.jpg)
![Immunohistochemistry-Paraffin: FMNL1 Antibody [NBP1-88461] - Staining of human bone marrow shows high expression.](http://images.novusbio.com/fullsize/FMNL1-Antibody-Immunohistochemistry-Paraffin-NBP1-88461-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: BP1 Antibody [NBP2-34130] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-34130-img0005.jpg "Immunocytochemistry/Immunofluorescence: BP1 Antibody [NBP2-34130] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunohistochemistry-Paraffin: BP1 Antibody [NBP2-34130] - Staining of human placenta shows cytoplasmic and nuclear positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/BP1-Antibody-Immunohistochemistry-Paraffin-NBP2-34130-img0006.jpg)
![Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73556] - Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None.](http://images.novusbio.com/fullsize/Goat-anti-Monkey-IgM-Heavy-Chain-Secondary-Antibody-[Biotin]-Western-Blot-NBP1-73556-img0003.jpg "Western Blot: Goat anti-Monkey IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73556] - Lane 1: Monkey IgM. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Biotin goat secondary antibody at 1:1,000 for 60 min at RT. Block: incubated with blocking buffer for 30 min t RT. Predicted/Observed size: 72 kDa, 72 kDa for Monkey IgM. Other band(s): None.")
![Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73379] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Rat-IgM-Secondary-Antibody-[Rhodamine]-Western-Blot-NBP1-73379-img0004.jpg "Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [Rhodamine] [NBP1-73379] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73679] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Goat-anti-Rat-IgM-Secondary-Antibody-[Biotin]-Western-Blot-NBP1-73679-img0004.jpg "Western Blot: Goat anti-Rat IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-73679] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (GOAT) Antibody 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
![Dot Blot: Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73847] - Dot Blot results of Rabbit anti-Canine IgM Heavy Chain Secondary antibody [HRP].Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit Anti-Dog IgM -HRP at 1ug/mL for 1hr at RT.](http://images.novusbio.com/fullsize/Rabbit-anti-Canine-IgM-Heavy-Chain-Secondary-Antibody-[HRP]-Dot-Blot-NBP1-73847-img0001.jpg "Dot Blot: Rabbit anti-Canine IgM Heavy Chain Secondary Antibody [HRP] [NBP1-73847] - Dot Blot results of Rabbit anti-Canine IgM Heavy Chain Secondary antibody [HRP].Dots are Dog IgM at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: 30 min at RT.Primary Antibody: none.Secondary Antibody: Rabbit Anti-Dog IgM -HRP at 1ug/mL for 1hr at RT.")
![Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72789-img0001.jpg "Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.")
![ELISA: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - ELISA Results of Rabbit anti-Mouse IgM Heavy Chain Secondary antibody tested against purified Mouse IgM. Each well was coated in duplicate with 10 ug of Mouse IgM. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Rabbit IgG mx10 HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72789-img0004.jpg)