| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0207A488 | |||
| Alexa Fluor 546 | DDX0207A546 | |||
| Alexa Fluor 647 | DDX0207A647 | |||
| Biotin | DDX0207B | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89546-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37265-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76680-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76465-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-97601SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-25259 | |||
| Azide and BSA Free | NBP2-25259SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-80826-25ul | |||
![Immunocytochemistry/Immunofluorescence: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Murine peyer patch: DC-SIGN](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Immunocytochemistry-Immunofluorescence-DDX0207-img0007.jpg "Immunocytochemistry/Immunofluorescence: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Murine peyer patch: DC-SIGN")
![Immunohistochemistry-Paraffin: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Tonsil](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Immunohistochemistry-Paraffin-DDX0207-img0008.jpg)
![Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis of Alexa Fluor (R) 647 conjugate of DDX0207. FACS staining of CD14+ CD209+ cells. (CD14+ cells cultured 5 days with GM CSF and IL-10)](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Flow-Cytometry-DDX0207-img0011.jpg)
![Flow Cytometry: DC-SIGN/CD209 Antibody (109H12.03) [DDX0207] - Analysis using the Alexa Fluor (R) 488 conjugate of DDX0207. A) Staining of DC-SIGN transfected HeLa cells. B) IF staining of DC-SIGN transfected cells with 109H12.03.](http://images.novusbio.com/fullsize/DC-SIGN-CD209-Antibody-109H12-03-Flow-Cytometry-DDX0207-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-89546] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89546-img0011.jpg "Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-89546] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0015.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0012.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human liver shows very weak cytoplasmic positivity in hepatocytes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0013.jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-89546] - Staining of human skeletal muscle shows moderate granular cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/FXR1-Antibody-Immunohistochemistry-Paraffin-NBP1-89546-img0014.jpg)
![Simple Western: FXR1 Antibody [NBP1-89546] - Simple Western lane view shows a specific band for FXR1 in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/FXR1-Antibody-Simple-Western-NBP1-89546-img0006.jpg)
![Simple Western: FXR1 Antibody [NBP1-89546] - Electropherogram image(s) of corresponding Simple Western lane view. FXR1 antibody was used at 1:100 dilution on RT-4 and U-251MG sp lysates(s).](http://images.novusbio.com/fullsize/FXR1-Antibody-Simple-Western-NBP1-89546-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunocytochemistry-Immunofluorescence-NBP2-37265-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Immunofluorescence analysis of Hela cells using CD68 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.")
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mouse mAb against U937 (1), Hela (2), HepG2 (3), Jurkat (4) cell lysate.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0004.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Analysis of bladder cancer tissues using CD68 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunohistochemistry-NBP2-37265-img0008.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Flow cytometric analysis of Hela cells using CD68 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Flow-Cytometry-NBP2-37265-img0006.jpg)
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mAb against human CD68 (AA: 42-155) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0002.jpg)
![Western Blot: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Western blot analysis using CD68 mAb against HEK293 (1) and CD68 (AA: 42-155)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Western-Blot-NBP2-37265-img0003.jpg)
![Immunohistochemistry-Paraffin: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Analysis of endometrial cancer tissues using CD68 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-Immunohistochemistry-NBP2-37265-img0007.jpg)
![ELISA: CD68/SR-D1 Antibody (3F7D3) [NBP2-37265] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD68-SR-D1-Antibody-3F7D3-ELISA-NBP2-37265-img0001.jpg)
![Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-DDX0380P-100-img0005.jpg "Immunohistochemistry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Staining of human lung frozen section.")
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of swine DC in skin and offerent lymph](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0007.jpg)
![Immunohistochemistry-Paraffin: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - Expression of C-type lectin receptors on human skin](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Immunohistochemistry-Paraffin-DDX0380P-100-img0004.jpg)
![Flow Cytometry: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - FACS staining of human in vitro generated DC's](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-Flow-Cytometry-DDX0380P-100-img0006.jpg)
![In-situ Hybridization: MMR/CD206/Mannose Receptor Antibody (122D2.08) [DDX0380P-100] - In vitro staining of swine skin DCS](http://images.novusbio.com/fullsize/MMR-CD206-Mannose-Receptor-Antibody-122D2-08-In-situ-Hybridization-DDX0380P-100-img0008.jpg)
![Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: TLR9 Antibody [NBP1-76680] - (G) Levels of TLR9 (NBP1-76680), COX4 (NB110-39115), MyD88, and NF-kB protein by Western blot. (H) Relative expression of TLR9 and COX4 protein. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680] - Immunofluorescence of TLR9 in Mouse Spleen tissue with TLR9 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76680_rabbit-polyclonal-tlr9-antibody-271120242013147.jpg)
![Western Blot: TLR9 Antibody [NBP1-76680] - Analysis of TLR9 in Jurkat cell lysate with TLR9 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0006.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: TLR9 Antibody [NBP1-76680] - (A) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB mRNA. (B) Levels of COX4 (NB110-39115), TLR9 (NBP1-76680), MyD88, and NF-kB protein by Western blot. Image collected and cropped by CiteAb from the following publication (frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/TLR9-Antibody-Western-Blot-NBP1-76680-img0007.jpg)
![Western Blot: TLR9 Antibody - BSA Free [NBP1-76680] - Levels of toll-like receptor (TLR) 9, myeloid differentiation factor 88 (MyD88), & nuclear factor (NF)-kappa B in lung tissues from animals with spontaneous breathing (CON), mechanical ventilation at normal tidal volume (NTV), high tidal volume (HTV), & ODN2088 pretreatment upon HTV stimulation (HTV + ODN). aP < 0.05 vs. CON group;bP < 0.05 vs. NTV group; & cP < 0.05 vs. HTV group. Image collected & cropped by CiteAb from the following publication (//www.frontiersin.org/article/10.3389/fimmu.2018.01477/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-76680_rabbit-polyclonal-tlr9-antibody-31020241535698.jpg)
![Immunocytochemistry/ Immunofluorescence: TLR9 Antibody - BSA Free [NBP1-76680] - Immunocytochemistry of TLR9 in Jurkat cells with TLR9 antibody at 2 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76680_rabbit-polyclonal-tlr9-antibody-2711202420243383.jpg)
![Western Blot: MyD88 Antibody [NBP1-76465] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/nbp1-76465_rabbit-polyclonal-myd88-antibody-2711202419463463.gif "<b>Genetic Strategies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - KO Validation in HeLa Cells. Loading: 10 ug of HeLa WT cell lysate or MyD88 KO cell lysate. Antibodies: MyD88 (2 ug/mL) and beta-actin (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Genetic Strategies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Western Blot Validation of MyD88 in human cell lines. Loading: 15 ug of lysates per lane. Antibodies: NBP1-76465 (2 ug/mL) 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Predicted band size: 35 kDa](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0010.jpg)
![Western Blot: MyD88 Antibody [NBP1-76465] - Animal Species ReactivityLoading: Lysates/proteins at 15 ug per lane.Antibodies: NBP1-76925 (2 ug/mL) or NBP1-76465 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0007.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 ug of lysates per lane.Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human TissuesLoading: 15 ug of lysates per lane.Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate.](http://images.novusbio.com/fullsize/MyD88-Antibody-Western-Blot-NBP1-76465-img0009.jpg)
![Immunocytochemistry/ Immunofluorescence: MyD88 Antibody - BSA Free [NBP1-76465] - Validation of MyD88 In HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling MyD88 with at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.](http://images.novusbio.com/fullsize/nbp1-76465_rabbit-polyclonal-myd88-antibody-2711202420392736.jpg)
![Immunocytochemistry/ Immunofluorescence: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunofluorescence Validation of MyD88 in Jurkat Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling MyD88 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunocytochemistry-immunofluorescence-22102025124527.gif)
![Immunohistochemistry: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunohistochemistry Validation of MyD88 in Human Colon. Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MYD88 antibody at 1 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunohistochemistry-22102025124757.jpg)
![Immunohistochemistry: Rabbit Polyclonal MyD88 Antibody [NBP1-76465] - Immunohistochemistry Validation of MyD88 in Mouse Heart. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-MYD88 antibody at 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/antibody/nbp1-76465_rabbit-polyclonal-myd88-antibody-imgenex-img-5499-immunohistochemistry-22102025125425.jpg)
![Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).](http://images.novusbio.com/fullsize/NLRP3-NALP3-Antibody-Nalpy3-b-Immunohistochemistry-Frozen-NBP1-97601-img0002.jpg "Immunohistochemistry-Frozen: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Staining of endogenous NLRP3/NALP3 in epithelial layer of human tonsil (frozen section) using NLRP3/NALP3 (human), mAb (Nalpy3-b). Method: 5uM frozen section of tissue are dried and fixed with aceton. Tissue is washed with PBS and incubated with NLRP3/NALP3 (human), mAb (Nalpy3-b).")
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3/NALP3 (NBP1-97601) infammasomes in VSMCs. Representative immunoblots for NLRP3/NALP3, Procaspase-1, cleaved caspase-1, IL-1 beta, and IL-18 in SCAP-overexpressing VSMCs. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-2552023153958.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression in VSMCs causes inflammation & lipid deposition in the aortas of SCAPD443N mice. (A & B) Representative images of an aortic root stained with Oil Red O in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding. (C) Quantification of plaque areas in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (D) Plasma levels of TC, TG, LDL-C & HDL-C in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (E) mRNA expression of proin?ammatory cytokines (IL-1 beta , IL-6, IL-18, & TNF-alpha ) as measured by qRT-PCR (n=4). (F) mRNA expression of pro-chemokine cytokines (MCP-1, MIP-1 alpha , MIP-1 beta , & CX3CL1) as measured by qRT-PCR (n=4). (G) mRNA expression of ICAM-1 & VCAM-1 in aortas as measured by qRT-PCR (n=4). (H) Representative immunoblots for NLRP3 proteins in SCAP+/+ mice & SCAPD443N mice (n=3). (I) Representative images of immuno?uorescence staining of NLRP3 proteins in the aortas of SCAPD443N mice & SCAP+/+ mice (n=5). The data are presented as the means±SD of 3-5 independent experiments. *P<0.05, vs. the SCAP+/+ group. Statistical significance was calculated for the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612244.jpg)
![Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3 in?ammasomes in VSMCs. (A) Representative immunoblots for NLRP3, Procaspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs. (B) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (C) Immunoblotting of immunoprecipitation with anti-SCAP or anti-NLRP3 in SCAP-overexpressing VSMCs. (D) Representative images of immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi(red) in control & SCAP-overexpressing VSMCs. (E) Representative immunoblot for NLRP3, Pro-caspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs treated with lycorine for 4 h. (F) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (G) Representative images of the immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi (red) in SCAP VSMCs after treatment with lycorine for 4 h. The data are presented as the means±SD of 3 independent experiments. *P<0.05, vs the control group. Statistical significance was calculated for to the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-97601_mouse-monoclonal-nlrp3-nalp3-antibody-nalpy3-b-31020241612193.jpg)
![Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Western-Blot-NBP2-75397-img0008.jpg "Western Blot: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of Iba1 on THP-1 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Staining AIF-1/Iba1 in SH-SY5Y cells using (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100, PBS.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunocytochemistry-Immunofluorescence-NBP2-75397-img0002.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence analysis of paraffin-embedded rat brain tissue labeling Iba1 with Rabbit anti-AIF-1/Iba1 antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4 , washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0015.jpg)
![Flow Cytometry: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of THP-1 cells withAIF-1:Iba1 Antibody (JM36-62) at 1:100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Flow-Cytometry-NBP2-75397-img0001.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human lung cancer tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0003.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0004.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0005.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse spleen tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0006.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded rat lung tissue using AIF-1/Iba1 Antibody (JM36-62). Counterstained with hematoxylin.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0007.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded human spleen tissue using anti-AIF-1/Iba1rabbit polyclonal antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4C and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0009.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Immunofluorescence staining of paraffin- embedded mouse brain tissue using anti-AIF-1/Iba1 antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with at 1/50 dilution for 10 hours at 4 and detected using Alexa Fluor(R) 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0010.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0011.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0012.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded human spleen tissue with Rabbit anti-AIF-1/Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0013.jpg)
![Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (JM36-62) [NBP2-75397] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Iba1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-JM36-62-Immunohistochemistry-Paraffin-NBP2-75397-img0014.jpg)
![Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of human lymph node tissue using TLR2 antibody at a concentration of 1 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Western-Blot-NBP2-25245-img0004.jpg "Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of human lymph node tissue using TLR2 antibody at a concentration of 1 ug/ml.")
![Immunohistochemistry-Paraffin: TLR2 Antibody (67N8F8) [NBP2-25245] - FFPE human esophageal squamous cell carcinoma stained with TLR2 antibody at 5 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Immunohistochemistry-Paraffin-NBP2-25245-img0003.jpg)
![Flow (Cell Surface): TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis using the FITC conjugate of NBP2-25259. Surface staining of stable HEK293/hTLR2 cells (red) and vector control cells (green) using TLR2 antibody at 5 ul/10^6 cells. Image using the Azide Free form of this antibody.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Flow-Cell-Surface-NBP2-25245-img0005.jpg)
![Western Blot: TLR2 Antibody (67N8F8) [NBP2-25245] - Analysis of (A) TLR2 partial recombinant protein, (B) transfected cell lysate, and (C) control 293T lysate using TLR2 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Western-Blot-NBP2-25245-img0001.jpg)
![Flow Cytometry: TLR2 Antibody (67N8F8) [NBP2-25245] - (A) Surface staining of transfected TLR2 HEK293 cells (red) and mock transfected cells (green) using TLR2 antibody at 1 ug/10^6 cells. FITC-conjugated anti-mouse IgG secondary was used for this test. (B) Surface staining of PPI-negative PBMC (monocyte-gated) using TLR2 antibody (red) and isotype control antibody (green) at 1 ug/10^6 cells. PE-conjugated anti-mouse IgG secondary was used for this test.](http://images.novusbio.com/fullsize/TLR2-Antibody-67N8F8-Flow-Cytometry-NBP2-25245-img0002.jpg)
![Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NBP1-80826-img0015.jpg "Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunocytochemistry/Immunofluorescence: TRAF-6 Antibody [NBP1-80826] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-80826-img0009.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0013.jpg)
![Western Blot: TRAF-6 Antibody [NBP1-80826] - Analysis in human cell line SH-SY5Y.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Western-Blot-NBP1-80826-img0014.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human lung shows moderate cytoplasmic positivity in macrophages.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0010.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human placenta shows weak to moderate cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0011.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-80826] - Staining of human skeletal muscle shows weak cytoplasmic positivity in myocytes.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-80826-img0012.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0023.jpg "Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Rat brain.TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - 59T xenograft, using TRAF6 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0012.jpg)
![Immunohistochemistry-Paraffin: TRAF-6 Antibody [NBP1-33357] - Mouse lung. TRAF6 antibody [N2C2], Internal diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunohistochemistry-Paraffin-NBP1-33357-img0020.jpg)
![Immunoprecipitation: TRAF-6 Antibody [NBP1-33357] - IP samples: K562 whole cell extract. A. Control with 4 ug of preimmune Rabbit IgG. B. Immunoprecipitation of TRAF6 protein by 4 ug TRAF6 antibody [N2C2], Internal 7.5 % SDS-PAGE. The immunoprecipitated TRAF6 protein was detected by TRAF6 antibody [N2C2], Internal diluted at 1:500.](http://images.novusbio.com/fullsize/TRAF-6-Antibody-Immunoprecipitation-NBP1-33357-img0016.jpg)
![Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-western-blot-26112025193619.jpg)
![TRAF6 antibody [N2C2], Internal detects TRAF6 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TRAF6 protein stained by TRAF6 antibody [N2C2], Internal (NBP1-33357) diluted at 1:100.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp1-33357_rabbit-polyclonal-traf-6-antibody-immunocytochemistry-immunofluorescence-2611202518503015.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0010.jpg "Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: MIF Antibody [NBP1-32192] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32192-img0002.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - Paraffin-embedded mouse brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0012.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate)A: Molt-4 15% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0005.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - : Paraffin-embedded rat brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0011.jpg)
![ELISA: MIF Antibody [NBP1-32192] - ELISA detection of MIF [N1C3] capture at a concentration of 5 ug/mL and MIF C-term antibody for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-ELISA-NBP1-32192-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32192_rabbit-polyclonal-mif-antibody-western-blot-141120251840435.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32192_rabbit-polyclonal-mif-antibody-western-blot-1411202518443421.jpg)