| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-62698-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50163-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45323 | |||
| Azide and BSA Free | NBP2-34589-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45323 | |||
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Western-Blot-H00001108-M01-img0009.jpg "Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Immunohistochemistry-Paraffin-H00001108-M01-img0006.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - CHD4 monoclonal antibody (M01), clone 4H4 Analysis of CHD4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Western-Blot-H00001108-M01-img0008.jpg)
![Sandwich ELISA: CHD4 Antibody (4H4) [H00001108-M01] - Detection limit for recombinant GST tagged CHD4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Sandwich-ELISA-H00001108-M01-img0007.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Analysis in human bone marrow and skeletal muscle tissues using NBP2-62698 antibody. Corresponding ADORA2A RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0005.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Analysis in human bone marrow and skeletal muscle tissues using NBP2-62698 antibody. Corresponding ADORA2A RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0009.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human caudate shows strong membranous positivity.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0006.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human bone marrow shows strong cytoplasmic positivity in hematopoietic cells.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0007.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human duodenum shows strong cytoplasmic positivity in enteroendocrine cells.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0008.jpg)
![Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NBP2-62698] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Adenosine-A2aR-Antibody-Immunohistochemistry-Paraffin-NBP2-62698-img0010.jpg)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-40799-img0007.jpg "Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - HEK293 cell lysates at 0.05ug/mL. Untransfected (lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.")
![Immunohistochemistry-Paraffin: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Staining of Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Immunohistochemistry-Paraffin-NB100-40799-img0005.jpg)
![Western Blot: Autoimmune Regulator/AIRE Antibody [NB100-40799] - Analysis of HEK293 cell lysates. Untransfected (Lane 3) and transfected with Human AIRE (lane 4). Data kindly provided by Prof. Part Peterson, University of Tartu, Estonia.](http://images.novusbio.com/fullsize/Autoimmune-Regulator-AIRE-Antibody-Western-Blot-NB100-40799-img0006.jpg)
![Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CHD4-Antibody-CHD4-3F2-4-Flow-Cytometry-NBP2-50163-img0001.jpg "Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-33458-img0014.jpg "Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - MDA-MB-231 xenograft, using HLA-DRA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-33458-img0003.jpg)
![Western Blot: HLA DRA Antibody [NBP1-33458] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA DRA antibody [N2C3] (NBP1-33458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33458_rabbit-polyclonal-hla-dra-antibody-9102024201570.jpg)
![HLA-DRA antibody [N2C3] detects HLA-DRA protein by immunofluorescent analysis. Sample: Raji cells were fixed in 100% Methenol for 5 min. Green: HLA-DRA protein stained by HLA-DRA antibody [N2C3] (NBP1-33458) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33458_rabbit-polyclonal-hla-dra-antibody-immunocytochemistry-immunofluorescence-261120251821566.jpg)
![Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0005.jpg "Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: CHD4 Antibody [NB100-57519] - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody NB100-57519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57519] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57519 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57520, NB100-57521), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). For blotting immunoprecipitated CHD4, NBP2-77358 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunoprecipitation-NB100-57519-img0006.jpg)
![Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57521-img0004.jpg "Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57521] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57521 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-NB100-57521-img0003.jpg)
![Western Blot: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Western blot analysis of HLA-DR on different lysates with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: HUT 102 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32435) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-278202416331238.jpg "Western Blot: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Western blot analysis of HLA-DR on different lysates with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution. Lane 1: Raji cell lysate Lane 2: HUT 102 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 29 kDa Observed band size: 33 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32435) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32435) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-298202414262655.jpg)
![Immunohistochemistry: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunofluorescence analysis of paraffin-embedded human stomach cancer tissue labeling HLA-DR with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-32435, green) at 1/100 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-298202414161847.jpg)
![Immunocytochemistry/ Immunofluorescence: HLA-DR Antibody (10-D8-R) [NBP3-32435] - Immunocytochemistry analysis of Daudi cells labeling HLA-DR with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HLA-DR antibody (NBP3-32435) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32435_mouse-hla-dr-mab-10-d8-r-2920241192357.jpg)
![Western Blot: IKB zeta Antibody [NB100-56390] - Analysis at 3 ug/ml in mouse RAW cell lysate.](http://images.novusbio.com/fullsize/IKB-zeta-Antibody-Western-Blot-NB100-56390-img0001.jpg "Western Blot: IKB zeta Antibody [NB100-56390] - Analysis at 3 ug/ml in mouse RAW cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (C8/468) [NBP2-32952] - Immunofluorescence staining of human PBMC cells using CD8 Mouse Monoclonal Antibody (C8/468) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Immunocytochemistry-Immunofluorescence-NBP2-32952-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD8 Antibody (C8/468) [NBP2-32952] - Immunofluorescence staining of human PBMC cells using CD8 Mouse Monoclonal Antibody (C8/468) followed by goat anti-mouse IgG-CF488 (green).")
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/468) [NBP2-32952] - Formalin-fixed, paraffin-embedded human tonsil stained with CD8 Mouse Monoclonal Antibody (C8/468).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Immunohistochemistry-Paraffin-NBP2-32952-img0003.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/468) [NBP2-32952] - Formalin-fixed, paraffin-embedded human lymph node stained with CD8 Mouse Monoclonal Antibody (C8/468).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Immunohistochemistry-Paraffin-NBP2-32952-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (C8/468) - Azide and BSA Free [NBP2-34589] - Immunofluorescence staining of human PBMC cells using CD8 Mouse Monoclonal Antibody (C8/468) followed by goat anti-mouse IgG-CF488 (green).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-34589-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD8 Antibody (C8/468) - Azide and BSA Free [NBP2-34589] - Immunofluorescence staining of human PBMC cells using CD8 Mouse Monoclonal Antibody (C8/468) followed by goat anti-mouse IgG-CF488 (green).")
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/468) - Azide and BSA Free [NBP2-34589] - Formalin-fixed, paraffin-embedded human lymph node stained with CD8 Mouse Monoclonal Antibody (C8/468).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34589-img0004.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (C8/468) - Azide and BSA Free [NBP2-34589] - Formalin-fixed, paraffin-embedded human tonsil stained with CD8 Mouse Monoclonal Antibody (C8/468).](http://images.novusbio.com/fullsize/CD8-Antibody-C8-468-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34589-img0003.jpg)