| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-24767SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-33220 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | MAB90782-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Allophycocyanin | NB7100APC | |||
| Biotin | NB7100B | |||
| DyLight 350 | NB7100UV | |||
| DyLight 405 | NB7100V | |||
| DyLight 488 | NB7100G | |||
| DyLight 550 | NB7100R | |||
| DyLight 594 | NB7100DL594 | |||
| DyLight 650 | NB7100C | |||
| DyLight 680 | NB7100FR | |||
| DyLight 755 | NB7100IR | |||
| FITC | NB7102 | |||
| HRP | NB7103 | |||
| Janelia Fluor 525 | NB7100JF525 | |||
| Janelia Fluor 669 | NB7100JF669 | |||
| PE | NB7100PE | |||
| PerCP | NB7100PCP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85345-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-76810SS | |||
| Azide and BSA Free | NBP2-80713 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-15133-25ul | |||
| Azide and BSA Free | NBP3-44362 | |||
![Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunohistochemistry-NB100-79807-img0011.jpg "Immunohistochemistry: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RCH1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Importin alpha 2/KPNA2 Antibody [NB100-79807] - Detection of human RCH1Importin alpha 2/KPNA2 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCH1 antibody NB100-79807 (lot 2) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. RCH1 was also immunoprecipitated by a previous lot of NB100-79807 (lot 1) and by rabbit anti-RCH1 antibody NB100-79808, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).](http://images.novusbio.com/fullsize/Importin-alpha-2-KPNA2-Antibody-Immunoprecipitation-NB100-79807-img0012.jpg)
![Flow Cytometry: TLR7 Antibody [NBP2-24767] - Intracellular staining by analysis of TLR7 in human PBMCs using this antibody at 2 ug/ml. Shaded histogrm represents cells alone; green represents rabbit IgG isotype control, and red represents the TLR7 antibody.](http://images.novusbio.com/fullsize/TLR7-Antibody-Flow-Cytometry-NBP2-24767-img0003.jpg "Flow Cytometry: TLR7 Antibody [NBP2-24767] - Intracellular staining by analysis of TLR7 in human PBMCs using this antibody at 2 ug/ml. Shaded histogrm represents cells alone; green represents rabbit IgG isotype control, and red represents the TLR7 antibody.")
![Western Blot: TLR7 Antibody [NBP2-24767] - Analysis of human TLR7 in Ramos cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody.](http://images.novusbio.com/fullsize/TLR7-Antibody-Western-Blot-NBP2-24767-img0001.jpg)
![Immunohistochemistry-Paraffin: TLR7 Antibody [NBP2-24767] - Human brain using 1:50 dilution of TLR7 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) was performed using Aperio AT2 (Leica Biosystems). Punctate staining of neurons was observed. Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TLR7-Antibody-Immunohistochemistry-Paraffin-NBP2-24767-img0005.jpg)
![Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Western-Blot-NB100-56532-img0007.jpg "Western Blot: IKK gamma Antibody (46B844) [NB100-56532] - Analysis using NEMO (IKKgamma) antibody. Lysate from human Jurkat cells probed with NEMO antibody at 2 ug/ml. The goat anti-mouse IgG1 HRP secondary and PicoTect ECL substrate solution were used for this test.")
![Flow Cytometry: IKK gamma Antibody (46B844) [NB100-56532] - Human Jurkat cells were probed using 0.1 ug of NEMO antibody (red) and 0.1 ug of isotype control (green), with shaded histogram representing cells alone and an anti-mouse IgG-FITC secondary antibody.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-46B844-Flow-Cytometry-NB100-56532-img0005.jpg)
![Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody (2096A) [MAB90782] - B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using B7-H1 Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.](http://images.novusbio.com/fullsize/B7-H1-PD-L1-CD274-Antibody-2096A-Immunohistochemistry-MAB90782-img0002.jpg "Immunohistochemistry: B7-H1/PD-L1/CD274 Antibody (2096A) [MAB90782] - B7-H1 was detected in perfusion fixed frozen sections of mouse thymus using B7-H1 Monoclonal Antibody at 1.7 ug/ml. Tissue was stained using Rabbit IgG VisUCyte HRP Polymer Antibody (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm and plasma membrane.")
![Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-Paraffin-NB110-93609-img0002.jpg "Immunohistochemistry-Paraffin: CD45 Antibody (5C16) [NB110-93609] - Correlation of MAP17 expression with inflammation (CD45) in human samples of tumors of different originMAP17 and CD45 staining of serial sections of human samples of tumors of different origin. MAP17 staining in all samples showed high expression levels, correlated with the inflammatory marker CD45. Image collected and cropped by CiteAb from the following publication (//www.oncotarget.com/fulltext/21651) licensed under a CC-BY license.")
![Immunohistochemistry: CD45 Antibody (5C16) [NB110-93609] - CD45 antibody (5C16) [NB110-93609] immunohistochemistry staining of paraffin sections of mouse kidney and liver tissues from LPS exposed animals.](http://images.novusbio.com/fullsize/CD45-Antibody-5C16-Immunohistochemistry-NB110-93609-img0001.jpg)
![Immunohistochemistry: CD45 Antibody (5C16) - Azide and BSA Free [NB110-93609] - Correlation of MAP17 expression with inflammation in human samples of Crohn's disease & psoriasisMAP17, CD45 & CD68 staining of histological slides of human samples of psoriasis & Crohn's disease. MAP17 staining in both inflammatory diseases showed high expression levels, correlated with the inflammatory markers CD45 & CD68. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29228712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-93609_rat-monoclonal-cd45-antibody-5c16-310202415171311.jpg)
![SDS-Page: Recombinant Human NLRP3/NALP3 Protein [H00114548-P01] - 12.5% SDS-PAGE stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-NLRP3-NALP3-Protein-SDS-Page-H00114548-P01-img0002.jpg "SDS-Page: Recombinant Human NLRP3/NALP3 Protein [H00114548-P01] - 12.5% SDS-PAGE stained with Coomassie Blue.")
![Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01), clone 3F9-2C2.Lane 1: B2M transfected lysate(13.7 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Western-Blot-H00000567-M01-img0015.jpg "Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody (M01), clone 3F9-2C2.Lane 1: B2M transfected lysate(13.7 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of monoclonal antibody to B2M on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Immunocytochemistry-Immunofluorescence-H00000567-M01-img0009.jpg)
![Immunohistochemistry-Paraffin: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Immunohistochemistry-Paraffin-H00000567-M01-img0011.jpg)
![Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi ( Cat # H00000567-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody (M01), clone 3F9-2C2 (Cat # H00000567-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Western-Blot-H00000567-M01-img0012.jpg)
![Western Blot: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - B2M monoclonal antibody (M01), clone 3F9-2C2 Analysis of B2M expression in U-2 OS.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Western-Blot-H00000567-M01-img0014.jpg)
![Proximity Ligation Assay: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Analysis of protein-protein interactions between CALR and B2M. HeLa cells were stained with anti-CALR rabbit purified polyclonal 1:1200 and anti-B2M mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-Proximity-Ligation-Assay-H00000567-M01-img0007.jpg)
![ELISA: beta 2-Microglobulin Antibody (3F9-2C2) [H00000567-M01] - Detection limit for recombinant GST tagged B2M is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-3F9-2C2-ELISA-H00000567-M01-img0016.jpg)
![Western Blot: STAT6 Antibody [NBP1-85345] - Analysis in human cell lines A-549 and U-251MG using Anti-STAT6 antibody. Corresponding STAT6 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.](http://images.novusbio.com/fullsize/STAT6-Antibody-Western-Blot-NBP1-85345-img0014.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: STAT6 Antibody [NBP1-85345] - Analysis in human cell lines A-549 and U-251MG using Anti-STAT6 antibody. Corresponding STAT6 RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining in human fallopian tube and skeletal muscle tissues using NBP1-85345 antibody. Corresponding STAT6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunohistochemistry-Paraffin-NBP1-85345-img0019.jpg)
![Simple Western: STAT6 Antibody [NBP1-85345] - Simple Western lane view shows a specific band for STAT6 in 0.2 mg/ml of RT-4 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/STAT6-Antibody-Simple-Western-NBP1-85345-img0006.jpg)
![Simple Western: STAT6 Antibody [NBP1-85345] - Electropherogram image(s) of corresponding Simple Western lane view. STAT6 antibody was used at 1:20 dilution on RT-4 lysate(s).](http://images.novusbio.com/fullsize/STAT6-Antibody-Simple-Western-NBP1-85345-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: STAT6 Antibody [NBP1-85345] - Staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85345-img0011.jpg)
![Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human Fallopian tube shows moderate to strong nuclear and cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunohistochemistry-Paraffin-NBP1-85345-img0015.jpg)
![Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunohistochemistry-Paraffin-NBP1-85345-img0016.jpg)
![Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human skin shows weak to moderate nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunohistochemistry-Paraffin-NBP1-85345-img0017.jpg)
![Immunohistochemistry-Paraffin: STAT6 Antibody [NBP1-85345] - Staining of human tonsil shows moderate nuclear and cytoplasmic positivity in non-germinal center cells.](http://images.novusbio.com/fullsize/STAT6-Antibody-Immunohistochemistry-Paraffin-NBP1-85345-img0018.jpg)
![Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunohistochemistry-Paraffin-NBP2-76810-img0003.jpg "Immunohistochemistry-Paraffin: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed paraffin-embedded sections of human kidney using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog #NBP2-76810) at 3 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyt(TM) HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm and nuclei.")
![Western Blot: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Total protein from human HeLa, THP-1 and U87 cell lines was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-PHD2 antibody in block buffer and detected with an anti-rabbit HRP secondary antibody using West Pico PLUS chemiluminescence detection reagent.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Western-Blot-NBP2-76810-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - EGLN1/PHD2 was detected in immersion fixed WM-115 human malignant melanoma cell line using Rabbit Anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810) at 3 ug/mL for 3 hours at room temperature. Cells were stained using the NorthernLight(TM) 557-conjugated Anti-Rabbit IgG secondary antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm and nuclei.](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Immunocytochemistry-Immunofluorescence-NBP2-76810-img0002.jpg)
![Flow Cytometry: EGLN1/PHD2 Antibody (2445B) [NBP2-76810] - Human Jurkat T Cell Leukemia Cell Line was stained with Rabbit anti-EGLN1/PHD2 Monoclonal Antibody (Catalog # NBP2-76810, filled histogram) or Rabbit IgG Isotype Control Antibody (Catalog # MAB1050, open histogram) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012).](http://images.novusbio.com/fullsize/EGLN1-PHD2-Antibody-2445B-Flow-Cytometry-NBP2-76810-img0001.jpg)
![Western Blot: CD5 Antibody (CL12334) [NBP3-15133] - Analysis in human cell line MOLT-4.](http://images.novusbio.com/fullsize/CD5-Antibody-CL12334-Western-Blot-NBP3-15133-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CD5 Antibody (CL12334) [NBP3-15133] - Staining of A-431 cells using the Anti-CD5 monoclonal antibody, showing localization in plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively.](http://images.novusbio.com/fullsize/CD5-Antibody-CL12334-Immunocytochemistry-Immunofluorescence-NBP3-15133-img0006.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Analysis in human lymph node and skeletal muscle tissues using NBP3-15133 antibody. Corresponding CD5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0005.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Staining of human chronic lymphocytic leukemia shows strong membranous positivity in tumor cells.](http://images.novusbio.com/fullsize/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0001.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Staining of human lymph node shows strong membranous positivity in germinal non-germinal center cells.](http://images.novusbio.com/fullsize/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0002.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0003.jpg)
![Immunohistochemistry-Paraffin: CD5 Antibody (CL12334) [NBP3-15133] - Staining of human small intestine shows strong membranous positivity in lymphoid cells.](http://images.novusbio.com/fullsize/CD5-Antibody-CL12334-Immunohistochemistry-Paraffin-NBP3-15133-img0004.jpg)
![Western Blot: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA by WB of Jurkat, MOLT-4, MJ, RPMI-8226, HeLa, KG-1, HEK293T, and SUP-M2 lysate. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG.Lower Panel: Rabbit anti-GAPDH.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Western-Blot-NBP2-76406-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA (red) in FFPE ovarian carcinoma by IHC-IF. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] NBP2-76406. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunocytochemistry-Immunofluorescence-NBP2-76406-img0004.jpg)
![Immunohistochemistry-Paraffin: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA in FFPE lung carcinoma by IHC. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunohistochemistry-NBP2-76406-img0002.jpg)
![Flow Cytometry: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Rabbit anti-VISTA recombinant monoclonal [BLR035F] (right) or isotype control (left). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Flow-Cytometry-NBP2-76406-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA in FFPE NCI-H226 cells by ICC. Antibody: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunocytochemistry-NBP2-76406-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: VISTA/B7-H5/PD-1H Antibody (BLR035F) [NBP2-76406] - Detection of human VISTA by WB of immunoprecipitates from Jurkat lysate. Antibodies: Rabbit anti-VISTA recombinant monoclonal [BLR035F] (NBP2-76406) and another rabbit anti-VISTA recombinant monoclonal.](http://images.novusbio.com/fullsize/VISTA-B7-H5-PD-1H-Antibody-BLR035F-Immunoprecipitation-NBP2-76406-img0006.jpg)