| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-62554-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11996-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81045-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35895-100ug | |||
| Carrier-Free | NBP2-35895-500ug | |||
| Carrier-Free | NBP2-35895-1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48030 | |||
![Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Colon, Carcinoma](http://images.novusbio.com/fullsize/XCR1-CCXCR1-Antibody-Immunohistochemistry-Paraffin-NLS156-img0006.jpg "Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Colon, Carcinoma")
![Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Analysis of anti-XCR1 antibody with human spleen at 2.5 ug/ml.](http://images.novusbio.com/fullsize/XCR1-CCXCR1-Antibody-Immunohistochemistry-NLS156-img0002.jpg)
![Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Skin, Malignant Melanoma](http://images.novusbio.com/fullsize/XCR1-CCXCR1-Antibody-Immunohistochemistry-Paraffin-NLS156-img0004.jpg)
![Immunohistochemistry-Paraffin: XCR1/CCXCR1 Antibody [NLS156] - Ovary, carcinoma](http://images.novusbio.com/fullsize/XCR1-CCXCR1-Antibody-Immunohistochemistry-Paraffin-NLS156-img0005.jpg)
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0015.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.")
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0014.jpg)
![Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).](http://images.novusbio.com/fullsize/HLA-G-Antibody-MEM-G-4-Western-Blot-NB500-533-img0001.jpg "Western Blot: HLA G Antibody (MEM-G/4) [NB500-533] - Analysis of HLA-G by the antibody MEM-G/4 on HLA-G1 transfectants (LCL-HLA-G1).")
![ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241141613.jpg "ELISA: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - IL-1 beta/IL-1F2 Antibody (NBP3-32464) in indirect ELISA. Indirect ELISA analysis of IL-1 beta was performed by coating wells of a 96-well plate with 50 µl per well of IL-1 beta antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with blocking buffer, and incubated with 50 µl per well of a mouse IL-1 beta monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 2 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.")
![Western Blot: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Western blot analysis of IL-1 beta/IL-1F2 on different lysates with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours add 300ng/mL BFA for 3 hours cell lysate Lysates/proteins at 10 ug/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32464) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-27820241631654.jpg)
![Immunohistochemistry: IL-1 beta/IL-1F2 Antibody (A7F7-R) [NBP3-32464] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-IL-1 beta/IL-1F2 antibody (NBP3-32464) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32464) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32464_mouse-il-1-beta-il-1f2-mab-a7f7-r-29820241420948.jpg)
![Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.](http://images.novusbio.com/fullsize/TfR-Transferrin-R-Antibody-MEM-189-Flow-Cytometry-NB500-493-img0001.jpg "Flow Cytometry: TfR (Transferrin R) Antibody (MEM-189) [NB500-493] - taining of CD71 in K562 cells with anti-CD71 (MEM-189) purified, GAM-APC.")
![Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Western-Blot-NBP2-62554-img0003.jpg "Western Blot: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Western Blot using anti-CD34 antibody My10. Jurkat cell extract (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of My10 (NBP2-62554) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for the canonical isoform of CD34 is 40.9kDa, though a second isoform at 35.2kDa is also present, with a 56 amino acid truncation at the C-terminus of the protein (Uniprot ID: Q64314). CD34 is also glycosylated at several positions. NBP2-62554 successfully detected both isoforms of human CD34.")
![Immunohistochemistry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Immunohistochemical staining of human placenta tissue using anti-CD34 antibody. My10 Anti-CD34 staining of paraffin embedded human placenta tissue using the rabbit-chimeric version of My10 (NBP2-62554). Antigen retreival was acheived by microwaving in citrate buffer (pH6), followed by blocking with protein block serum-free buffer. Primary antibody incubation with NBP2-62554 was carried out at 4 ug/ml for 30 minutes. Samples were then incubated with an anti-rabbit IgG HRP secondary antibody for 20 mins followed by DAB (3,3'-diaminobenzidine), and counter-staining with haemotoxylin. Strong endothelial staining of within villi may be observed. Recommended concentration, 2-4 ug/ml.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Immunohistochemistry-NBP2-62554-img0002.jpg)
![Flow Cytometry: CD34 Antibody (My10) - Chimeric [NBP2-62554] - Flow-cytometry using anti-CD34 antibody My10. Human leukocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of My10 (NBP2-62554, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD34-Antibody-My10-Chimeric-Flow-Cytometry-NBP2-62554-img0001.jpg)
![Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23316_mouse-plexin-a1-mab-m535-19920231856930.jpg "Western Blot: Plexin A1 Antibody (M535) [NBP3-23316] - Western blots showing mouse recombinant Plexin A1 extracellular domain (lanes 1-4). These blots were probed with rabbit polyclonal anti-Plexin A1 (NBP3-23309) at 1:250 (lane 1) and 1:1000 (lane 2) or with mouse monoclonal anti-Plexin A1 (NBP3-23316) at 1:250 (lane 3) and 1:1000 (lane 4).")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody (M535) [NBP3-23316] - Immunocytochemical labeling of Plexin A1 in aldehyde fixed and NP-40 permeabilized human NCI-H1299 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-Plexin A1 (NBP3-23316). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23316_mouse-plexin-a1-mab-m535-19920231856954.jpg)
![Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).](http://images.novusbio.com/fullsize/nbp3-23309_rabbit-plexin-a1-pab-1992023185690.jpg "Western Blot: Plexin A1 Antibody [NBP3-23309] - Western blots showing Cos-7 cells transfected with mouse Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin A1 immunoprecipitated from Myc-tagged Plexin A1 transfected cells using anti-Myc (lane 4) or anti-Plexin A1 (NBP3-23309; lane 5). These blots were probed with either the affinity purified anti-Plexin A1 (NBP3-23309; lanes 1 & 2) or with mouse monoclonal anti-Myc (lanes 3-5).")
![Immunocytochemistry/Immunofluorescence: Plexin A1 Antibody [NBP3-23309] - Immunocytochemical double labeling using anti-Myc mouse monoclonal and anti-Plexin A1 rabbit polyclonal (NBP3-23309) antibodies in Cos-7 cells mock transfected (A,D) or transfected with Myc-tagged mouse Plexin A1 construct (B,E). The specificity of the binding in E was demonstrated by using Plexin A1 peptide in the presence of this anti-Plexin A1 antibody (C,F).](http://images.novusbio.com/fullsize/nbp3-23309_rabbit-plexin-a1-pab-119202316331615.jpg)
![Western Blot: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 (NBP3-11996) at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCR5-p-Ser337-Antibody-V14-2-Chimeric-Western-Blot-NBP3-11996-img0001.jpg "Western Blot: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - K562 cell lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 (NBP3-11996) at 2ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CCR5 [p Ser337] Antibody (V14/2) - Chimeric [NBP3-11996] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of V14/2 (NBP3-11996) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11996, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CCR5-p-Ser337-Antibody-V14-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11996-img0002.jpg)
![Flow Cytometry: CCR5 [p Ser337] Antibody (V14/2) - Chimeric - Azide and BSA Free [NBP3-11996] - U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11996 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11996_rabbit-monoclonal-ccr5-p-ser337-antibody-v14-2-chimeric-74202522113035.png)
![Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-BBM-1-Chimeric-Western-Blot-NBP2-81045-img0003.jpg "Western Blot: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Western Blot using anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cell lysate (35ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of BBM.1 [NBP2-81045] at 0.03 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for Beta-2 microglobulin is 13.7 kDa. NBP2-81045 successfully detected human Beta-2 microglobulin in both HeLa cell extract and human duodenum samples.")
![Flow Cytometry: beta 2-Microglobulin Antibody (BBM.1) - Chimeric [NBP2-81045] - Flow-cytometry using the anti-Beta-2 microglobulin antibody BBM.1 [NBP2-81045]. HeLa cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of BBM.1 (NBP2-81045, blue line) at a concentration of 10 ug/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody at 2 ug/ml and cells analyzed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/beta-2-Microglobulin-Antibody-BBM-1-Chimeric-Flow-Cytometry-NBP2-81045-img0002.jpg)
![Immunocytochemistry /Immunofluorescence: beta 2-Microglobulin Antibody (BBM.1) - Chimeric - Azide and BSA Free [NBP2-81045] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton stained with NBP2-81045 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom: NBP2-81045, DAPI, merged channels and a negative control. The negative control was stained with unimmunized rabbit IgG followed by Alexa Fluor 488 secondary antibody.](http://images.novusbio.com/fullsize/nbp2-81045_rabbit-monoclonal-beta-2-microglobulin-antibody-bbm-1-chimeric-74202522113026.png)
![Functional: Human IL1 beta Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL1-beta-Protein-Functional-NBP2-35895-img0001.jpg "Functional: Human IL1 beta Protein [NBP2-35895]")
![SDS-Page: Human IL-1 beta/IL-1F2 Protein [NBP2-35895]](http://images.novusbio.com/fullsize/Human-IL-1-beta-IL-1F2-Protein-SDS-Page-NBP2-35895-img0002.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (IGA/515) - Azide and BSA Free [NBP2-47839] - Formalin-fixed, paraffin-embedded human Lymphoma stained with CD79A Antibody (IGA/515).](http://images.novusbio.com/fullsize/CD79A-Antibody-IGA-515-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47839-img0002.jpg "Immunohistochemistry-Paraffin: CD79A Antibody (IGA/515) - Azide and BSA Free [NBP2-47839] - Formalin-fixed, paraffin-embedded human Lymphoma stained with CD79A Antibody (IGA/515).")