| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03403-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier-Free | NBP2-35076-1mg | |||
| Carrier-Free | NBP2-35076-100ug | |||
| Carrier-Free | NBP2-35076-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61726-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-05122-0.025mg | |||
![Western Blot: ORAI3 Antibody [NBP2-82013] - Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.](http://images.novusbio.com/fullsize/ORAI3-Antibody-Western-Blot-NBP2-82013-img0001.jpg "Western Blot: ORAI3 Antibody [NBP2-82013] - Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 1, (B) 2 and (C) 4 ug/mL.")
![Immunohistochemistry: ORAI3 Antibody - BSA Free [NBP2-82013] - Immunohistochemistry of ORAI3 in mouse spleen tissue with ORAI3 antibody at 2 u/mL.](http://images.novusbio.com/fullsize/nbp2-82013_rabbit-polyclonal-orai3-antibody-271120241918645.gif)
![Immunocytochemistry/ Immunofluorescence: ORAI3 Antibody - BSA Free [NBP2-82013] - Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp2-82013_rabbit-polyclonal-orai3-antibody-2711202420441150.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytochrome b245 alpha Antibody - BSA Free [NBP3-03403] - Immunofluorescence analysis of MCF7 cells using Cytochrome b245 alpha Rabbit pAb (A10694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03403_rabbit-polyclonal-cytochrome-b245-alpha-antibody-52202519294549.jpg "Immunocytochemistry/ Immunofluorescence: Cytochrome b245 alpha Antibody - BSA Free [NBP3-03403] - Immunofluorescence analysis of MCF7 cells using Cytochrome b245 alpha Rabbit pAb (A10694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: Cytochrome b245 alpha Antibody - BSA Free [NBP3-03403] - Immunofluorescence analysis of PC-12 cells using Cytochrome b245 alpha Rabbit pAb (A10694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03403_rabbit-polyclonal-cytochrome-b245-alpha-antibody-5220251931371.jpg)
![Immunocytochemistry/ Immunofluorescence: Cytochrome b245 alpha Antibody - BSA Free [NBP3-03403] - Immunofluorescence analysis of NIH/3T3 cells using Cytochrome b245 alpha Rabbit pAb (A10694) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03403_rabbit-polyclonal-cytochrome-b245-alpha-antibody-52202519313425.jpg)
![Western Blot: Cytochrome b245 alpha Antibody - BSA Free [NBP3-03403] - Western blot analysis of RAW264.7, using Cytochrome b245 alpha antibody (A10694) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-03403_rabbit-polyclonal-cytochrome-b245-alpha-antibody-52202519294554.jpg)
![Western Blot: Cytochrome b245 alpha Antibody - BSA Free [NBP3-03403] - Western blot analysis of Rat lung, using Cytochrome b245 alpha antibody (A10694) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-03403_rabbit-polyclonal-cytochrome-b245-alpha-antibody-5220251931352.jpg)
![Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0002.jpg "Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in HeLa cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0004.jpg)
![Western Blot: MIF Antibody (4E4) [NBP1-04319] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Jurkat cell lysateLane 2.: THP-1 cell lysateLane 3.: HeLa cell lysateLane 4.: U937 cell lysate](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Western-Blot-NBP1-04319-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: MIF Antibody (4E4) [NBP1-04319] - Analysis of MIF in Balb/3T3 cells. The cell was stained at (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).](http://images.novusbio.com/fullsize/MIF-Antibody-4E4-Immunocytochemistry-Immunofluorescence-NBP1-04319-img0003.jpg)
![SDS-Page: Human TNF-alpha Protein [NBP2-35076]](http://images.novusbio.com/fullsize/Human-TNF-alpha-Protein-SDS-Page-NBP2-35076-img0001.jpg "SDS-Page: Human TNF-alpha Protein [NBP2-35076]")
![Western Blot: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis using CD1C mAb against human CD1C (AA: extra 18-302) recombinant protein. (Expected MW is 58.1 kDa)](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-Western-Blot-NBP2-61726-img0004.jpg "Western Blot: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis using CD1C mAb against human CD1C (AA: extra 18-302) recombinant protein. (Expected MW is 58.1 kDa)")
![Immunohistochemistry-Paraffin: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis of cervical cancer tissues using CD1C mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-Immunohistochemistry-NBP2-61726-img0003.jpg)
![Flow Cytometry: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis of Ramos cells using CD1C mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-Flow-Cytometry-NBP2-61726-img0002.jpg)
![Western Blot: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Analysis using CD1C mAb against HEK293 (1) and CD1C (AA: extra 18-302)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-Western-Blot-NBP2-61726-img0005.jpg)
![ELISA: CD1c/BDCA-1 Antibody (2A7C11) [NBP2-61726] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/CD1c-BDCA-1-Antibody-2A7C11-ELISA-NBP2-61726-img0001.jpg)
![Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP1-05122-img0011.jpg "Immunohistochemistry: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining in human kidney. Strong staining of plasma of nephritis kidney was seen. Dilution: 1:75.")
![Western Blot: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Lane 1: purified C3, lane 2: purifed C3 reduced/DTT, lane 3: purified C3a , lane 4: EDTA-plasma. Lane 5: zymosan-activated serum](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Western-Blot-NBP1-05122-img0004.jpg)
![Immunohistochemistry-Paraffin: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - Staining of human kidney. Strong staining of veins of nephritis kidney was seen. Dilution: 1:150.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Immunohistochemistry-Paraffin-NBP1-05122-img0007.jpg)
![Sandwich ELISA Capture: Complement C3a Antibody (K13/16-5.7) - BSA Free [NBP1-05122] - The calibration curve of a sandwich assay for Complement C3a using NBP1-05122 as the capture antibody and NBP2-23496 as the biotinylated detection antibody.](http://images.novusbio.com/fullsize/Complement-C3a-Antibody-K13-16-5-7-BSA-Free-Sandwich-ELISA-Capture-NBP1-05122-img0005.jpg)
![Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Knockout-Validated-NBP1-03250-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Lysates of THP-1 (WT and SYK KO) were prepared and 30 ug of protein were processed for immunoblot with SYK Antibody (SYK-01). The Ponceau stained transfer is shown. Antibody dilution used: 1/500. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).")
![<b>Genetic Strategies Validation. </b>Western Blot: SYK Antibody (SYK-01) [NBP1-03250] - Western blot of extracts from Human PBMC, SYK Antibody (SYK-01)](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP1-03250-img0002.jpg)
![Immunohistochemistry-Paraffin: SYK Antibody (SYK-01) [NBP1-03250] - Staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunohistochemistry-Paraffin-NBP1-03250-img0001.jpg)
![Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Western-Blot-NBP3-09107-img0001.jpg "Western Blot: SYK Antibody (SYK-01) [NBP3-09107] - Analysis (non-reducing conditions) of whole cell lysate of RAMOS human Burkitt lymphoma cell line (1), RBL rat basophilic leukemia cell line (2) and HeLa human cervix carcinoma cell line (3, 4). Lane 1, 2: immunostaining with anti-Syk (SYK-01) Lane 3, 4: immunostaining with anti-human Cytokeratin 18, Lane 5: negative control")
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human HeLa cell line using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SYK Antibody (SYK-01) [NBP3-09107] - Analysis of Syk in human primary fibroblasts using anti-Syk (SYK-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).](http://images.novusbio.com/fullsize/SYK-Antibody-SYK-01-Immunocytochemistry-Immunofluorescence-NBP3-09107-img0002.jpg)
![Western Blot: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-278202415295599.jpg "Western Blot: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Western blot analysis of CD68/SR-D1 on different lysates with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. Lane 1: THP-1 cell lysate Lane 2: U-937 cell lysate Lane 3: U-87 MG cell lysate Lane 4: Jurkat cell lysate (negative) Lysates/proteins at 10 ug/Lane. Predicted band size: 37 kDa Observed band size: 100-150 kDa Exposure time: 2 minutes 45 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32163) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32163) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-298202414115445.jpg)
![Immunocytochemistry/ Immunofluorescence: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Immunocytochemistry analysis of THP-1 (positive) and Jurkat (negative) labeling CD68/SR-D1 with Mouse anti-CD68/SR-D1/SR-D1 antibody (NBP3-32163) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD68/SR-D1 antibody (NBP3-32163) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-29202410234233.jpg)
![Flow Cytometry: CD68/SR-D1 Antibody (A3C3-R) [NBP3-32163] - Flow cytometric analysis of THP-1 (positive) and Jurkat (negative) cells labeling CD68/SR-D1. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32163, 1μg/mL) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32163_mouse-cd68-sr-d1-mab-a3c3-r-392024884626.jpg)
![Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-278202415295515.jpg "Western Blot: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Western blot analysis of Complement C5 on different lysates with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/2,000 dilution. Lane 1: HepG2 cell lysate (20 ug/Lane) Lane 2: Human liver tissue lysate (40 ug/Lane) Predicted band size: 188 kDa Observed band size: 188/72 kDa Exposure time: 43 seconds; ECL 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32209) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32209) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29820241435282.jpg)
![Immunocytochemistry/ Immunofluorescence: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Immunocytochemistry analysis of HepG2 cells labeling Complement C5 with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Complement C5 antibody (NBP3-32209) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-2920241192373.jpg)
![Flow Cytometry: Complement C5 Antibody (PSH04-01) [NBP3-32209] - Flow cytometric analysis of HepG2 cells labeling Complement C5. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32209, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32209_rabbit-complement-c5-mab-psh04-01-29202416372127.jpg)
![Western Blot: CD21 Antibody (PD00-23) [NBP3-32135] - Western blot analysis of CD21 on Raji cell lysates with Rabbit anti-CD21 antibody (NBP3-32135) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32135) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-278202415295561.jpg "Western Blot: CD21 Antibody (PD00-23) [NBP3-32135] - Western blot analysis of CD21 on Raji cell lysates with Rabbit anti-CD21 antibody (NBP3-32135) at 1/1,000 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 113 kDa Observed band size: 130 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32135) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD21 Antibody (PD00-23) [NBP3-32135] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD21 antibody (NBP3-32135) at 1/4,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32135) at 1/4,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-298202413585521.jpg)
![Immunocytochemistry/ Immunofluorescence: CD21 Antibody (PD00-23) [NBP3-32135] - Immunocytochemistry analysis of Raji cells labeling CD21 with Rabbit anti-CD21 antibody (NBP3-32135) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD21 antibody (NBP3-32135) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-29202410484856.jpg)
![Flow Cytometry: CD21 Antibody (PD00-23) [NBP3-32135] - Flow cytometric analysis of Raji cells labeling CD21. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32135, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32135_rabbit-cd21-mab-pd00-23-29202416453281.jpg)
![ELISA: IL-10 Antibody (JES3-12G8) [NBP2-27577] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (Clone JES3-12G8) followed by Streptavidin-HRP.](http://images.novusbio.com/fullsize/IL-10-Antibody-JES3-12G8-ELISA-NBP2-27577-img0001.jpg "ELISA: IL-10 Antibody (JES3-12G8) [NBP2-27577] - Standard curve generated with Rat Anti-Human IL-10-UNLB ( Clone JES3-9D7) and Rat Anti-Human IL-10-BIOT (Clone JES3-12G8) followed by Streptavidin-HRP.")