| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48028 | |||
| Azide and BSA Free | NBP2-34637-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56127SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-18057SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-43299-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-70291 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-63062-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37349-0.025ml | |||
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB p65 [p Ser276] Antibody-Western Blot-NBP1-77807-img0010.jpg "Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method.")
![Immunohistochemistry: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - Diluted to1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Immunohistochemistry-NBP1-77807-img0007.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS529 shows phospho p65 staining in carcinoma cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0005.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0006.jpg)
![Western Blot: RelA/NFkB p65 [p Ser276] Antibody [NBP1-77807] - TNF Induces phosphorylation of p65 in KBM-5 cells.](http://images.novusbio.com/fullsize/RelA-NFkB-p65-[p-Ser276]-Antibody-Western-Blot-NBP1-77807-img0008.jpg)
![Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Western-Blot-NB600-477-img0006.jpg "Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.")
![Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Paraffin-embedded mouse brain sections (IKK beta KO or IKK beta WT) were stained with IKK beta (green). Note: during staining, remember to add 0.1-0.3% Triton-100 in the antibody incubation buffer. See PMID 25253847 for further details regarding the experiemental conditions. Image from verified customer review.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NB600-477-img0003.jpg)
![Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Human placenta tissue.](http://images.novusbio.com/fullsize/IKK-beta-Antibody-Immunohistochemistry-Paraffin-NB600-477-img0004.jpg)
![Western Blot: CD79A Antibody (HM47/A9) [NBP2-44738] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Western-Blot-NBP2-44738-img0005.jpg "Western Blot: CD79A Antibody (HM47/A9) [NBP2-44738] - Western Blot Analysis of Raji cell lysate using CD79A Antibody (HM47/A9).")
![Immunocytochemistry/Immunofluorescence: CD79A Antibody (HM47/A9) [NBP2-44738] - Immunofluorescence Analysis of Raji cells labeling CD79a with CD79A Antibody (HM47/A9). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunocytochemistry-Immunofluorescence-NBP2-44738-img0003.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM47/A9) [NBP2-44738] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD79A Antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunohistochemistry-Paraffin-NBP2-44738-img0004.jpg)
![Flow Cytometry: CD79A Antibody (HM47/A9) [NBP2-44738] - Flow Cytometric Analysis of Raji cells. CD79A Antibody (HM47/A9) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Flow-Cytometry-NBP2-44738-img0006.jpg)
![Immunohistochemistry-Paraffin: CD79A Antibody (HM47/A9) [NBP2-44738] - Formalin-fixed, paraffin-embedded human tonsil stained with CD79a antibody (HM47/A9).](http://images.novusbio.com/fullsize/CD79A-Antibody-HM47-A9-Immunohistochemistry-Paraffin-NBP2-44738-img0001.jpg)
![Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127] - FFPE tissue section of human tonsil stained for CD40 expression using this antibody at 1:2000. Staining is seen in the dendritic cells. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/CD40-TNFRSF5-Antibody-Immunohistochemistry-Paraffin-NB100-56127-img0003.jpg "Immunohistochemistry-Paraffin: CD40/TNFRSF5 Antibody [NB100-56127] - FFPE tissue section of human tonsil stained for CD40 expression using this antibody at 1:2000. Staining is seen in the dendritic cells. Hematoxylin-eosin counterstain.")
![Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0010.jpg "Western Blot: MIF Antibody [NBP1-32192] - Various tissue extracts (50 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Immunocytochemistry/Immunofluorescence: MIF Antibody [NBP1-32192] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32192-img0002.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - Paraffin-embedded mouse brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0012.jpg)
![Western Blot: MIF Antibody [NBP1-32192] - Sample (30 ug of whole cell lysate)A: Molt-4 15% SDS PAGE, antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/MIF-Antibody-Western-Blot-NBP1-32192-img0005.jpg)
![Immunohistochemistry-Paraffin: MIF Antibody [NBP1-32192] - : Paraffin-embedded rat brain. MIF antibody [N1C3] diluted at 1:500.](http://images.novusbio.com/fullsize/MIF-Antibody-Immunohistochemistry-Paraffin-NBP1-32192-img0011.jpg)
![ELISA: MIF Antibody [NBP1-32192] - ELISA detection of MIF [N1C3] capture at a concentration of 5 ug/mL and MIF C-term antibody for detection at a concentration of 1.5 ug/mL.](http://images.novusbio.com/fullsize/MIF-Antibody-ELISA-NBP1-32192-img0008.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp1-32192_rabbit-polyclonal-mif-antibody-western-blot-141120251840435.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with MIF antibody [N1C3] (NBP1-32192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32192_rabbit-polyclonal-mif-antibody-western-blot-1411202518443421.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Western-Blot-NBP1-20180-img0006.jpg "Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - WB on brain lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:5000 incubated at 4C overnight.")
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min.Blocking: 0.2% LFDM in TBST filtered thru 0.2 um.Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen.Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer.Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0022.jpg)
![Western Blot: P2X7/P2RX7 Antibody [NBP1-20180] - Rat brain lysate using Rabbit antibody to P2RX7 at 1:500 dilution. Incubated 30 min at RT with shake. Blocking: 0.5% LFDM in 1x PBS containing 0.1% Tween-20](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Western-Blot-NBP1-20180-img0005.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0010.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0011.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0012.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat DRG. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0013.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0014.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0015.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0016.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0017.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0018.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min using. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0019.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat brain. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0020.jpg)
![Immunohistochemistry-Paraffin: P2X7/P2RX7 Antibody [NBP1-20180] - IHC-P on paraffin sections of rat cerebellum. The animal was perfused using Autoperfuser at a pressure of 130 mmHg with 300 ml 4% FA being processed for paraffin embedding. HIER: Tris-EDTA, pH 9 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer; DAB chromogen: Candela DAB chromogen. Primary antibody: dilution 1: 2000, incubated 30 min at RT using Autostainer. Sections were counterstained with Harris Hematoxylin](http://images.novusbio.com/fullsize/P2X7-P2RX7-Antibody-Immunohistochemistry-Paraffin-NBP1-20180-img0021.jpg)
![Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Western-Blot-NBP3-13648-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membranes were blotted with AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat brain. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse spinal cord. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:200. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0001.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Mouse hippocampus. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - Rat cerebellum. AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Paraffin-NBP3-13648-img0003.jpg)
![Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - AIF-1/Iba1 antibody [HL22] detects AIF-1/Iba1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse brain. Green: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22] (NBP3-13648) diluted at 1:100. Red: AIF-1/Iba1 stained by AIF-1/Iba1 antibody [HL22-RT] diluted at 1:100. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/AIF-1-Iba1-Antibody-HL22-Immunohistochemistry-Frozen-NBP3-13648-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: AIF-1/Iba1 Antibody (HL22) [NBP3-13648] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Iba1 stained by Iba1 antibody [HL22] diluted at 1:500.Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunocytochemistry-immunofluorescence-244202411033..jpg)
![Iba1 antibody [HL22] detects Iba1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Iba1 stained by Iba1 antibody [HL22] (NBP3-13648) conjugated with DyLight488 diluted at 1:200.Blue: Fluoroshield with DAPI .Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-immunohistochemistry-paraffin-261120251850420.jpg)
![Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Iba1 antibody [HL22] (NBP3-13648) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-13648_rabbit-aif-1-iba1-mab-hl22-western-blot-2611202518454020.jpg)
![Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-18057-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD4 Antibody [NBP3-18057] - U-251 MG cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with CD4 Antibody (NBP3-18057) at 1ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Immunohistochemistry-Paraffin: CD4 Antibody [NBP3-18057] - Analysis of a FFPE tissue section of human spleen using 1:200 dilution of CD4 antibody (NBP3-18057). The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.](http://images.novusbio.com/fullsize/CD4-Antibody-Immunohistochemistry-Paraffin-NBP3-18057-img0001.jpg)
![Flow Cytometry: CD4 Antibody [NBP3-18057] - An intracellular stain was performed on Jurkat cells with CD4 Antibody NBP3-18057 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).](http://images.novusbio.com/fullsize/CD4-Antibody-Flow-Cytometry-NBP3-18057-img0003.jpg)
![Immunohistochemistry-Paraffin: Rabbit CD4 pAb [NBP3-18057] - Immunofluorescence staining of human tonsil FFPE tissue. Antibody diluted 1:50 (Catalog # NBP3-18057AF647) in 3% BSA and incubated over night at 4 °C. Heat mediated antigen retrieval at pH 9. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp3-18057_rabbit-cd4-pab-immunohistochemistry-paraffin-49202411280..jpg)
![Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.](http://images.novusbio.com/fullsize/T-bet-TBX21-Antibody-39D-Western-Blot-NBP1-43299-img0004.jpg "Western Blot: T-bet/TBX21 Antibody (39D) [NBP1-43299] - CD4+ T cells were sorted from mouse splenocytes, then activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-stimulation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-stimulated (right) cells were probed with Anti-T-bet Purified at 2 ug/ml, and revealed with Anti-Mouse IgG HRP.")
![Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Western-Blot-NBP2-02472-img0002.jpg "Western Blot: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BTK (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BTK.")
![Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] Staining of COS7 cells transiently transfected by pCMV6-ENTRY BTK.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP2-02472-img0009.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded prostate tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0008.jpg)
![Flow Cytometry: BTK Antibody (3A2) [NBP2-02472] - HEK293T cells transfected with either pCMV6-ENTRY BTK.(Red) or empty vector control plasmid (Blue) were immunostained with anti-BTK mouse monoclonal, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Flow-Cytometry-NBP2-02472-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: BTK Antibody (3A2) [NBP2-02472] - Immunofluorescent staining of HT29 cells using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunocytochemistry-Immunofluorescence-NBP2-02472-img0003.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0004.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0005.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Carcinoma of liver tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0006.jpg)
![Immunohistochemistry-Paraffin: BTK Antibody (3A2) [NBP2-02472] - Staining of paraffin-embedded Kidney tissue using anti-BTK mouse monoclonal antibody.](http://images.novusbio.com/fullsize/BTK-Antibody-3A2-Immunohistochemistry-Paraffin-NBP2-02472-img0007.jpg)
![CyTOF-ready: Lysozyme Antibody (BGN/0696/5B1) [NB100-63062] - Human bone marrow after RBC lysis was stained with surface antibodies, fixed, permeabilized with methanol, stained with anti-lysozyme NB100-63062, and gated for the above populations per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/Lysozyme-Antibody-BGN-0696-5B1-CyTOF-ready-NB100-63062-img0001.jpg "CyTOF-ready: Lysozyme Antibody (BGN/0696/5B1) [NB100-63062] - Human bone marrow after RBC lysis was stained with surface antibodies, fixed, permeabilized with methanol, stained with anti-lysozyme NB100-63062, and gated for the above populations per Nat Med. 2020. 26:408-417. CyTOF image submitted by a verified customer review.")
![Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Western-Blot-NBP2-37349-img0004.jpg "Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mouse mAb against Hela (1), MOLT4 (2), HEK293 (3), A549 (4), Jurkat (5), K562 (6), Cos7 (7), PC-12 (8) and NIH/3T3 (9) cell lysate.")
![Immunocytochemistry/Immunofluorescence: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of Hela cells using IL2RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Immunocytochemistry-Immunofluorescence-NBP2-37349-img0005.jpg)
![Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of bladder cancer tissues using IL2RA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Immunohistochemistry-NBP2-37349-img0007.jpg)
![Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against human IL2RA recombinant protein. (Expected MW is 37.5 kDa)](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Western-Blot-NBP2-37349-img0002.jpg)
![Western Blot: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis using IL2RA mAb against HEK293 (1) and IL2RA (AA: 34-139)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Western-Blot-NBP2-37349-img0003.jpg)
![Immunohistochemistry-Paraffin: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Analysis of rectum cancer tissues using IL2RA mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-Immunohistochemistry-NBP2-37349-img0006.jpg)
![ELISA: CD25/IL-2R alpha Antibody (1B5D12) [NBP2-37349] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/CD25-IL-2-R-alpha-Antibody-1B5D12-ELISA-NBP2-37349-img0001.jpg)