| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89364-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11976-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08997-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12000-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75189-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12080-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12086-0.025mg | |||
![Western Blot: Fc gamma RIIB/CD32b Antibody (110) [NBP2-89364] - Lane A: Daudi Whole Cell Lysate Lane B: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa](http://images.novusbio.com/fullsize/Fc-gamma-RIIB-CD32b-Antibody-110-Western-Blot-NBP2-89364-img0002.jpg "Western Blot: Fc gamma RIIB/CD32b Antibody (110) [NBP2-89364] - Lane A: Daudi Whole Cell Lysate Lane B: THP1 Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:34 kDa Observed band size:38 kDa")
![Immunohistochemistry-Paraffin: Fc gamma RIIB/CD32b Antibody (110) [NBP2-89364] - Staining of human lymphonode showing membrane staining of B cells and some other mononuclear cell (1:10000).](http://images.novusbio.com/fullsize/Fc-gamma-RIIB-CD32b-Antibody-110-Immunohistochemistry-Paraffin-NBP2-89364-img0001.jpg)
![Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-278202415212472.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/2,000 dilution. Lane 1: TF-1 cell lysate Lane 2: Jurkat cell lysate (negative) Lane 3: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32147) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202410334035.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-51) [NBP3-32147] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32147, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32147_rabbit-siglec-3-cd33-mab-psh02-51-29202416453237.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0005.jpg "Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.")
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Analysis of anti-CCR1 antibody with human spleen, lymphocytes.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0001.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Analysis of anti-CCR1 antibody with human colon, carcinoma.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0003.jpg)
![Immunohistochemistry-Paraffin: CCR1 Antibody [NLS1242] - Anti-CCR1 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.](http://images.novusbio.com/fullsize/CCR1-Antibody-Immunohistochemistry-Paraffin-NLS1242-img0004.jpg)
![Western Blot: CD-M6PR Antibody (PSH01-06) [NBP3-32555] - Western blot analysis of CD-M6PR on different lysates with Rabbit anti-CD-M6PR antibody (NBP3-32555) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: PC-12 cell lysate (20 ug/Lane) Lane 3: Mouse kidney tissue lysate (40 ug/Lane) Lane 4: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 37/46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32555) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32555_rabbit-cd-m6pr-mab-psh01-06-278202416285299.jpg "Western Blot: CD-M6PR Antibody (PSH01-06) [NBP3-32555] - Western blot analysis of CD-M6PR on different lysates with Rabbit anti-CD-M6PR antibody (NBP3-32555) at 1/1,000 dilution. Lane 1: A549 cell lysate (20 ug/Lane) Lane 2: PC-12 cell lysate (20 ug/Lane) Lane 3: Mouse kidney tissue lysate (40 ug/Lane) Lane 4: Rat kidney tissue lysate (40 ug/Lane) Predicted band size: 31 kDa Observed band size: 37/46 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32555) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD-M6PR Antibody (PSH01-06) [NBP3-32555] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-CD-M6PR antibody (NBP3-32555) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32555) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32555_rabbit-cd-m6pr-mab-psh01-06-298202414333687.jpg)
![Western Blot: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-278202415212431.jpg "Western Blot: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Western blot analysis of Siglec-3/CD33 on different lysates with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: TF-1 cell lysate Lane 3: HL-60 cell lysate Lane 4: Jurkat cell lysate (negative) Lane 5: SK-MEL-28 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 70 kDa Exposure time: 3 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32146) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Immunocytochemistry analysis of TF-1 (positive) and Jurkat (negative) labeling Siglec-3/CD33 with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Siglec-3/CD33 antibody (NBP3-32146) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202410484839.jpg)
![Flow Cytometry: Siglec-3/CD33 Antibody (PSH02-50) [NBP3-32146] - Flow cytometric analysis of TF-1 cells labeling Siglec-3/CD33. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32146, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32146_rabbit-siglec-3-cd33-mab-psh02-50-29202416453211.jpg)
![Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (RM80) - Chimeric [NBP3-11976] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RM80 (NBP3-11976) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11976, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-RM80-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11976-img0001.jpg "Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (RM80) - Chimeric [NBP3-11976] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RM80 (NBP3-11976) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11976, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Immunocytochemistry/Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric [NBP3-08997] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of AT130-5 (NBP3-08997) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-08997, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/Fc-gamma-RII-CD32-Antibody-AT130-5-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-08997-img0001.jpg "Immunocytochemistry/Immunofluorescence: Fc gamma RII/CD32 Antibody (AT130-5) - Chimeric [NBP3-08997] - Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of AT130-5 (NBP3-08997) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (1 ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-08997, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor(R) 488 secondary antibody.")
![Western Blot: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Jurkat (A), Molt4 (B), K562 (C) and Daudi (D) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RC-10 (NBP3-12000) at 0.0003 ug/ml, 0.001 ug/ml, 0.003 ug/ml and unknown ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CCR5-Antibody-RC-10-Chimeric-Western-Blot-NBP3-12000-img0001.jpg "Western Blot: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Jurkat (A), Molt4 (B), K562 (C) and Daudi (D) cell lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of RC-10 (NBP3-12000) at 0.0003 ug/ml, 0.001 ug/ml, 0.003 ug/ml and unknown ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CCR5 Antibody (RC-10) - Chimeric [NBP3-12000] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of RC-10 (NBP3-12000) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12000, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CCR5-Antibody-RC-10-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12000-img0002.jpg)
![Flow Cytometry: CCR5 Antibody (RC-10) - Chimeric - Azide and BSA Free [NBP3-12000] - Paraformaldehyde fixed human peripheral blood monocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12000 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12000_rabbit-monoclonal-ccr5-antibody-rc-10-chimeric-7420252214129.png)
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0003.jpg "Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Rhesus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.")
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Human lymphocytes were stained with an isotype control (panel A) or the rabbit-chimeric version of Eptratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0001.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (hL22 (Epratuzumab)) - Chimeric [NBP2-75189] - Flow-cytometry using anti-CD22 antibody Epratuzumab. Cynomolgus monkey lymphocytes were stained with an isotype control (NBP2-52636, panel A) or the rabbit-chimeric version of Epratuzumab (NBP2-75189, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-hL22-Epratuzumab-Chimeric-Flow-Cytometry-NBP2-75189-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric [NBP3-12080] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 (NBP3-12080) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12080, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD81-Antibody-1-3-3-22-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12080-img0001.jpg "Immunocytochemistry/Immunofluorescence: CD81 Antibody (1.3.3.22) - Chimeric [NBP3-12080] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 1.3.3.22 (NBP3-12080) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12080, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CD81 Antibody (1.3.3.22) - Chimeric - Azide and BSA Free [NBP3-12080] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12080 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12080_rabbit-monoclonal-cd81-antibody-1-3-3-22-chimeric-74202522141241.png)
![Immunocytochemistry/Immunofluorescence: CCR5 Antibody (T21/8) - Chimeric [NBP3-12086] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version T21/8 (NBP3-12086) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12086, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CCR5-Antibody-T21-8-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12086-img0001.jpg "Immunocytochemistry/Immunofluorescence: CCR5 Antibody (T21/8) - Chimeric [NBP3-12086] - Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version T21/8 (NBP3-12086) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12086, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: CCR5 Antibody (T21/8) - Chimeric - Azide and BSA Free [NBP3-12086] - Paraformaldehyde fixed U937 cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12086 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12086_rabbit-monoclonal-ccr5-antibody-t21-8-chimeric-74202522141224.png)
![Immunocytochemistry/Immunofluorescence: CD46 Antibody (M037) [NBP3-23191] - Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (NBP3-23191). The antibody was detected using goat anti-mouse Ig:DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23191_mouse-cd46-mab-m037-19920231759445.jpg "Immunocytochemistry/Immunofluorescence: CD46 Antibody (M037) [NBP3-23191] - Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (NBP3-23191). The antibody was detected using goat anti-mouse Ig:DyLight(R) 594.")
![Immunocytochemistry/Immunofluorescence: CD46 Antibody (M037) [NBP3-23191] - Immunocytochemical labeling of CD46 in aldehyde fixed human NCIH1915 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-CD46 (NBP3-23191). The antibody was detected using goat anti-mouse Ig:DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23191_mouse-cd46-mab-m037-19920231240371.jpg)
![Western Blot: CD46 Antibody (M037) [NBP3-23191] - Western blot of native lysates from NCI-H2052 mesothelial cells (lane 1), SK-MES-1 squamous carcinoma (lane 2), MDA-MB-231 breast carcinoma (lane 3), MeWo melanoma (lane 4), A431 skin adenocarcinoma (lane 5), LNCaP prostate cancer cells (lane 6), and MCF7 breast cancer cells (lane 7). The blot was probed with mouse monoclonal anti-CD46 (NBP3-23191) at 1:1000.](http://images.novusbio.com/fullsize/nbp3-23191_mouse-cd46-mab-m037-19920231240375.jpg)