| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76740SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12566-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56439SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76287-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-35252-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92814-0.02ml | |||
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0007.jpg "<b>Expression of Tagged Proteins Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation with Recombinant Protein.Loading: 30 ng of human IL-21 recombinant protein per lane.Antibodies: IL-21 NBP1-76740 (0.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.")
![<b>Expression of Tagged Proteins Validation. </b>Immunocytochemistry/Immunofluorescence: IL-21 Antibody [NBP1-76740] - Immunocytochemistry Validation of IL-21 in K562 Cells.Immunocytochemical analysis of K562 cells using anti-IL-21 antibody (NBP1-76740) at 2 ug/mL. Cells were fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.](http://images.novusbio.com/fullsize/IL-21-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76740-img0008.jpg)
![Western Blot: IL-21 Antibody [NBP1-76740] - Western Blot Validation in Human, Mouse and Rat Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Western Blot: IL-21 Antibody [NBP1-76740] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: IL-21 NBP1-76740 (1 ug/mL), IL-21 from Company B (5 ug/mL), beta-actin (5 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.](http://images.novusbio.com/fullsize/IL-21-Antibody-Western-Blot-NBP1-76740-img0005.jpg)
![Western Blot: IL-21 Antibody - BSA Free [NBP1-76740] - Figure 3 Western Blot Validation in Human HL-60 Cell LysateLoading: 15 u of lysates per lane.Antibodies: IL-21 (1 u/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.(A) Absence of blocking peptide(B) Presence of blocking peptide.](http://images.novusbio.com/fullsize/nbp1-76740_rabbit-polyclonal-il-21-antibody-271120241937752.jpg)
![Western Blot: NLRX1 Antibody [NBP3-12566] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:108 kDaObserved band size:110 kDa](http://images.novusbio.com/fullsize/NLRX1-Antibody-Western-Blot-NBP3-12566-img0001.jpg "Western Blot: NLRX1 Antibody [NBP3-12566] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:108 kDaObserved band size:110 kDa")
![Immunohistochemistry-Paraffin: NLRX1 Antibody [NBP3-12566] - Staining of human NLRX1 in human urinary bladder with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/NLRX1-Antibody-Immunohistochemistry-Paraffin-NBP3-12566-img0003.jpg)
![Immunoprecipitation: NLRX1 Antibody [NBP3-12566] - Lane A:0.5 mg MCF7 Whole Cell Lysate4 uL anti-NLRX1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-NLRX1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 108 kDaObserved band size :110 kDa](http://images.novusbio.com/fullsize/NLRX1-Antibody-Immunoprecipitation-NBP3-12566-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: NLRX1 Antibody [NBP3-12566] - Staining of NLRX1 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12566 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12566_rabbit-polyclonal-nlrx1-antibody-23520251628127.png)
![Western Blot: IL-21 R Antibody [NB100-56439] - Analysis of IL-21 R in 30 ug of Raji cell lysate using this antibody.](http://images.novusbio.com/fullsize/IL-21-R-Antibody-Western-Blot-NB100-56439-img0003.jpg "Western Blot: IL-21 R Antibody [NB100-56439] - Analysis of IL-21 R in 30 ug of Raji cell lysate using this antibody.")
![Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody [NBP1-33728] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2BP2 protein stained by IGF2BP2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33728-img0007.jpg "Immunocytochemistry/Immunofluorescence: IMP2/IGF2BP2 Antibody [NBP1-33728] - Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IGF2BP2 protein stained by IGF2BP2 antibody diluted at 1:500. Blue: Hoechst 33343 staining.")
![Immunohistochemistry-Paraffin: IMP2/IGF2BP2 Antibody [NBP1-33728] - Paraffin-embedded Colon ca, using antibody at 1:250 dilution.](http://images.novusbio.com/fullsize/IMP2-IGF2BP2-Antibody-Immunohistochemistry-Paraffin-NBP1-33728-img0008.jpg)
![Western Blot: IMP2/IGF2BP2 Antibody [NBP1-33728] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IMP2/IGF2BP2 antibody (NBP1-33728) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33728_rabbit-polyclonal-imp2-igf2bp2-antibody-91020242001046.jpg)
![Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by Western blot. Image from verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182) antibody.](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells, treated with TNFa or calf intestinal phosphatase (CIP).](http://images.novusbio.com/fullsize/p38-alpha-[p-Tyr182]-Antibody-Western-Blot-NB100-82097-img0005.jpg)
![Immunohistochemistry-Paraffin: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of paraffin-embedded human breast carcinoma tissue, using NB100-82097(left) or the same antibody preincubated with blocking peptide(right).](http://images.novusbio.com/fullsize/nb100-82097_rabbit-polyclonal-p38-alpha-p-tyr182-antibody-10320252332107.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0005.jpg "Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 3 antibody diluted by 1:10000.")
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0001.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: PC-12 7. 5% SDS PAGE; antibody diluted at 1:10000.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Western-Blot-NBP2-15356-img0002.jpg)
![Immunoprecipitation: Alpha Actinin 3 Antibody [NBP2-15356] - Immunoprecipitation of alpha Actinin 3 protein from HeLa whole cell extracts using 5 ug of alpha Actinin 3 antibody. Western blot analysis was performed using alpha Actinin 3 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Alpha-Actinin-3-Antibody-Immunoprecipitation-NBP2-15356-img0004.jpg)
![Western Blot: Alpha Actinin 3 Antibody [NBP2-15356] - Sample (30 ug of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE NBP2-15356 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp2-15356_rabbit-polyclonal-alpha-actinin-3-antibody-9102024205628.jpg)
![Western Blot: Calcineurin A Antibody [NB110-40553] - Detection of human and mouse PPP3CA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP3CA antibody NB110-40553 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-Western-Blot-NB110-40553-img0009.jpg "Western Blot: Calcineurin A Antibody [NB110-40553] - Detection of human and mouse PPP3CA by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPP3CA antibody NB110-40553 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Immunohistochemistry-Paraffin: Calcineurin A Antibody [NB110-40553] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- PPP3CA used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-Immunohistochemistry-NB110-40553-img0008.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: Calcineurin A Antibody [NB110-40553] - Detection of human Calcineurin A (PPP3CA) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Calcineurin A antibody NB110-40553 used for IP at 3 ug per reaction. Calcineurin A was also immunoprecipitated by rabbit anti-Calcineurin A antibody NB110-40552. For blotting immunoprecipitated Calcineurin A, NB110-40553 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Calcineurin-A-Antibody-Immunoprecipitation-NB110-40553-img0010.jpg)
![Western Blot: NLRX1 Antibody [NBP1-76287] - Rat spleen tissue cell lysate with NOD5 antibody at (A) 1 and (B) 2 ug/mL.](http://images.novusbio.com/fullsize/NLRX1-Antibody-Western-Blot-NBP1-76287-img0003.jpg "Western Blot: NLRX1 Antibody [NBP1-76287] - Rat spleen tissue cell lysate with NOD5 antibody at (A) 1 and (B) 2 ug/mL.")
![Immunohistochemistry: NLRX1 Antibody - BSA Free [NBP1-76287] - Immunohistochemistry of NLRX1 in rat spleen tissue with NLRX1 antibody at 10 u/mL.](http://images.novusbio.com/fullsize/nbp1-76287_rabbit-polyclonal-nlrx1-antibody-2711202420195311.jpg)
![Immunocytochemistry/ Immunofluorescence: NLRX1 Antibody - BSA Free [NBP1-76287] - Immunofluorescence of NLRX1 in rat spleen tissue with NLRX1 antibody at 20 u/mL.](http://images.novusbio.com/fullsize/nbp1-76287_rabbit-polyclonal-nlrx1-antibody-27112024195844.jpg)
![Immunocytochemistry/ Immunofluorescence: EMR1 Antibody [NBP3-35252] - Immunofluorescence analysis of THP-1 cells using EMR1 Rabbit pAb at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35252_rabbit-emr1-pab-9122024151953.jpg "Immunocytochemistry/ Immunofluorescence: EMR1 Antibody [NBP3-35252] - Immunofluorescence analysis of THP-1 cells using EMR1 Rabbit pAb at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunocytochemistry/ Immunofluorescence: EMR1 Antibody [NBP3-35252] - Immunofluorescence analysis of RAW264.7 cells using EMR1 Rabbit pAb at dilution of 1:25 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35252_rabbit-emr1-pab-912202415175282.jpg)
![Western Blot: EMR1 Antibody [NBP3-35252] - Western blot analysis of lysates from Mouse lung, using EMR1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-35252_rabbit-emr1-pab-912202414163077.jpg)
![Immunocytochemistry/ Immunofluorescence: EMR1 Antibody [NBP3-35252] - Immunofluorescence analysis of paraffin-embedded Mouse spleen using EMR1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-35252_rabbit-emr1-pab-912202415111836.jpg)
![Western Blot: ECSIT Antibody - BSA Free [NBP2-92814] - Western blot analysis of extracts of HepG2 cells, using ECSIT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-42202514251823.jpg%3Ft=1690982332 "Western Blot: ECSIT Antibody - BSA Free [NBP2-92814] - Western blot analysis of extracts of HepG2 cells, using ECSIT antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.")
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of L929 cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-42202514275299.jpg%3Ft=1690982383)
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of U2OS cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-422025143047.jpg%3Ft=1690982390)
![Immunocytochemistry/ Immunofluorescence: ECSIT Antibody - BSA Free [NBP2-92814] - Immunofluorescence analysis of H9C2 cells using ECSIT Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92814_rabbit-polyclonal-ecsit-antibody-422025143045.jpg%3Ft=1690982372)
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)
![Western Blot: EMR1 Antibody - BSA Free [NBP3-46535] - HL-60 cells were subjected to SDS PAGE followed by western blot with (EMR1 Antibody) at dilution of 1:1000](http://images.novusbio.com/fullsize/nbp3-46535_rabbit-emr1-pab-bsa-free-28420251154181.jpg "Western Blot: EMR1 Antibody - BSA Free [NBP3-46535] - HL-60 cells were subjected to SDS PAGE followed by western blot with (EMR1 Antibody) at dilution of 1:1000")
![Immunohistochemistry: EMR1 Antibody - BSA Free [NBP3-46535] - Immunohistochemistry of paraffin-embedded mouse spleen tissue slide using (EMR1 Antibody) at dilution of 1:200](http://images.novusbio.com/fullsize/nbp3-46535_rabbit-emr1-pab-bsa-free-284202511524730.jpg)
![Immunocytochemistry/ Immunofluorescence: EMR1 Antibody - BSA Free [NBP3-46535] - Immunofluorescent analysis of ( 10% Formaldehyde ) fixed Raw264.7 cells using (EMR1 antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Rabbit IgG(H+L)](http://images.novusbio.com/fullsize/nbp3-46535_rabbit-emr1-pab-bsa-free-28420251146315.jpg)