| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08830 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-61863-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79916 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79919 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50163-0.025ml | |||
![Western Blot: CHD4 Antibody (3F2/4) [NBP3-07505] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Western-Blot-NBP3-07505-img0003.jpg "Western Blot: CHD4 Antibody (3F2/4) [NBP3-07505] - Western Blot Analysis of SK-Br3 cell lysate using CHD4 Mouse Monoclonal Antibody (3F2/4).")
![Immunocytochemistry/Immunofluorescence: CHD4 Antibody (3F2/4) [NBP3-07505] - Confocal immunofluorescence image of MCF-7 cells using CHD4 Mouse Monoclonal Antibody (3F2/4) stained in Green (CF488) and Phalloidin is used to label the membranes.](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Immunocytochemistry-Immunofluorescence-NBP3-07505-img0001.jpg)
![Immunohistochemistry-Paraffin: CHD4 Antibody (3F2/4) [NBP3-07505] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CHD4 Mouse Monoclonal Antibody (3F2/4).](http://images.novusbio.com/fullsize/CHD4-Antibody-3F2-4-Immunohistochemistry-Paraffin-NBP3-07505-img0002.jpg)
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0005.jpg "Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.")
![Immunocytochemistry/Immunofluorescence: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Immunocytochemistry-Immunofluorescence-NBP2-61863-img0003.jpg)
![Flow Cytometry: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-ELISA-NBP2-61863-img0002.jpg)
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mAb against human HLA-DRA (AA: 26-254) recombinant protein. (Expected MW is 51.5 kDa)](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0004.jpg)
![Western Blot: HLA DRA Antibody (1C11A5) [NBP2-61863] - Analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-Western-Blot-NBP2-61863-img0006.jpg)
![ELISA: HLA DRA Antibody (1C11A5) [NBP2-61863] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-1C11A5-ELISA-NBP2-61863-img0001.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0002.jpg "Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (40 ug) from HeLa cells transfected with CTCF siRNA (lane 2) and from an untransfected control (lane 1) were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.")
![Chromatin Immunoprecipitation: CTCF Antibody [NBP2-59228] - ChIP was performed with the antibody against CTCF on sheared chromatin from 4,000,000 HeLa cells. A titration consisting of 1, 2, 5 and 10 ug of antibody per ChIP experiment was analyzed. IgG (2 ug/IP) was used as a negative IP control. Quantitative PCR was performed with optimized primers for the H19 imprinting control region, and a specific region in the GAPDH gene, used as positive controls, and for the MYOD1 gene and the Sat2 satellite repeat region, used as a negative control. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/CTCF-Antibody-Chromatin-Immunoprecipitation-NBP2-59228-img0004.jpg)
![Western Blot: CTCF Antibody [NBP2-59228] - Whole cell extracts (25 ug) from HeLa cells were analysed by Western blot using the antibody against CTCF diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (kDa) is shown on the left.](http://images.novusbio.com/fullsize/CTCF-Antibody-Western-Blot-NBP2-59228-img0001.jpg)
![ELISA: CTCF Antibody [NBP2-59228] - To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against CTCF. The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be >1:500,000.](http://images.novusbio.com/fullsize/CTCF-Antibody-ELISA-NBP2-59228-img0003.jpg)
![Immunofluorescence: CTCF Antibody [NBP2-59228] - HeLa cells were stained with the antibody against CTCF and with DAPI. Cells were fixed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labeled with the CTCF antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.](http://images.novusbio.com/fullsize/CTCF-Antibody-Immunofluorescence-NBP2-59228-img0005.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Western-Blot-H00001108-M01-img0009.jpg "Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of CHD4 expression in transfected 293T cell line by CHD4 monoclonal antibody (M01), clone 4H4.Lane 1: CHD4 transfected lysate(220 KDa).Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CHD4 Antibody (4H4) [H00001108-M01] - Analysis of monoclonal antibody to CHD4 on formalin-fixed paraffin-embedded human transitional cell carcinoma. Antibody concentration 3 ug/ml.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Immunohistochemistry-Paraffin-H00001108-M01-img0006.jpg)
![Western Blot: CHD4 Antibody (4H4) [H00001108-M01] - CHD4 monoclonal antibody (M01), clone 4H4 Analysis of CHD4 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Western-Blot-H00001108-M01-img0008.jpg)
![Sandwich ELISA: CHD4 Antibody (4H4) [H00001108-M01] - Detection limit for recombinant GST tagged CHD4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/CHD4-Antibody-4H4-Sandwich-ELISA-H00001108-M01-img0007.jpg)
![Western Blot: Complement Component C1qA Antibody (C1QA/2954) - Azide and BSA Free [NBP2-79919] - Western Blot Analysis of human PC3 cell lysate using C1QA Mouse Monoclonal Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Azide-and-BSA-Free-Western-Blot-NBP2-79919-img0003.jpg "Western Blot: Complement Component C1qA Antibody (C1QA/2954) - Azide and BSA Free [NBP2-79919] - Western Blot Analysis of human PC3 cell lysate using C1QA Mouse Monoclonal Antibody (C1QA/2954).")
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2954) - Azide and BSA Free [NBP2-79919] - Formalin-fixed, paraffin-embedded human Liver stained with C1QA Mouse Monoclonal Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79919-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2954) - Azide and BSA Free [NBP2-79919] - Formalin-fixed, paraffin-embedded human Kidney stained with C1QA Mouse Monoclonal Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79919-img0001.jpg)
![Protein Array: Complement Component C1qA Antibody (C1QA/2954) - Azide and BSA Free [NBP2-79919] - Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Azide-and-BSA-Free-Protein-Array-NBP2-79919-img0004.jpg)
![Western Blot: Complement Component C1qA Antibody (C1QA/2953) - Azide and BSA Free [NBP2-79916] - Western Blot Analysis of PC-3 cell lysate using C1QA Mouse Monoclonal Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Azide-and-BSA-Free-Western-Blot-NBP2-79916-img0003.jpg "Western Blot: Complement Component C1qA Antibody (C1QA/2953) - Azide and BSA Free [NBP2-79916] - Western Blot Analysis of PC-3 cell lysate using C1QA Mouse Monoclonal Antibody (C1QA/2953).")
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2953) - Azide and BSA Free [NBP2-79916] - Formalin-fixed, paraffin-embedded human Liver stained with C1QA Mouse Monoclonal Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79916-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2953) - Azide and BSA Free [NBP2-79916] - Formalin-fixed, paraffin-embedded human Liver stained with C1QA Mouse Monoclonal Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-79916-img0001.jpg)
![Protein Array: Complement Component C1qA Antibody (C1QA/2953) - Azide and BSA Free [NBP2-79916] - - and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Azide-and-BSA-Free-Protein-Array-NBP2-79916-img0004.jpg)
![Western Blot: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Western Blot Analysis of PC-3 cell lysate using Complement Component C1qA Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Western-Blot-NBP2-79782-img0004.jpg "Western Blot: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Western Blot Analysis of PC-3 cell lysate using Complement Component C1qA Antibody (C1QA/2953).")
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Formalin-fixed, paraffin-embedded human Liver stained with Complement Component C1qA Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Immunohistochemistry-Paraffin-NBP2-79782-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Formalin-fixed, paraffin-embedded human Liver stained with Complement Component C1qA Antibody (C1QA/2953).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Immunohistochemistry-Paraffin-NBP2-79782-img0001.jpg)
![Protein Array: Complement Component C1qA Antibody (C1QA/2953) [NBP2-79782] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Complement Component C1qA Antibody (C1QA/2953). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2953-Protein-Array-NBP2-79782-img0003.jpg)
![Western Blot: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Western Blot Analysis of human PC3 cell lysate using Complement Component C1qA Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Western-Blot-NBP2-79785-img0004.jpg "Western Blot: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Western Blot Analysis of human PC3 cell lysate using Complement Component C1qA Antibody (C1QA/2954).")
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Formalin-fixed, paraffin-embedded human Liver stained with Complement Component C1qA Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Immunohistochemistry-Paraffin-NBP2-79785-img0002.jpg)
![Immunohistochemistry-Paraffin: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Formalin-fixed, paraffin-embedded human Kidney stained with Complement Component C1qA Antibody (C1QA/2954).](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Immunohistochemistry-Paraffin-NBP2-79785-img0001.jpg)
![Protein Array: Complement Component C1qA Antibody (C1QA/2954) [NBP2-79785] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Complement Component C1qA Antibody (C1QA/2954). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Complement-Component-C1qA-Antibody-C1QA-2954-Protein-Array-NBP2-79785-img0003.jpg)
![Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.](http://images.novusbio.com/fullsize/CHD4-Antibody-CHD4-3F2-4-Flow-Cytometry-NBP2-50163-img0001.jpg "Flow Cytometry: CHD4 Antibody (CHD4 3F2/4) [NBP2-50163] - An intracellular stain was performed on HeLa cells with CHD4 Antibody (CHD4 3F2/4) NBP2-50163PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.")
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-33458-img0014.jpg "Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - Human breast carcinoma. HLA-DRA stained by HLA-DRA antibody [N2C3] diluted at 1:10000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.")
![Immunohistochemistry-Paraffin: HLA DRA Antibody [NBP1-33458] - MDA-MB-231 xenograft, using HLA-DRA antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/HLA-DRA-Antibody-Immunohistochemistry-Paraffin-NBP1-33458-img0003.jpg)
![Western Blot: HLA DRA Antibody [NBP1-33458] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HLA DRA antibody [N2C3] (NBP1-33458) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33458_rabbit-polyclonal-hla-dra-antibody-9102024201570.jpg)
![HLA-DRA antibody [N2C3] detects HLA-DRA protein by immunofluorescent analysis. Sample: Raji cells were fixed in 100% Methenol for 5 min. Green: HLA-DRA protein stained by HLA-DRA antibody [N2C3] (NBP1-33458) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/fullsize/nbp1-33458_rabbit-polyclonal-hla-dra-antibody-immunocytochemistry-immunofluorescence-261120251821566.jpg)
![Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0005.jpg "Western Blot: CHD4 Antibody [NB100-57519] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Western Blot: CHD4 Antibody [NB100-57519] - Detection of Human CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CHD4/Mi2 beta antibody NB100-57519 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57519-img0004.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57519] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57519 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57520, NB100-57521), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). For blotting immunoprecipitated CHD4, NBP2-77358 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-Immunoprecipitation-NB100-57519-img0006.jpg)
![Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.](http://images.novusbio.com/fullsize/CHD4-Antibody-Western-Blot-NB100-57521-img0004.jpg "Western Blot: CHD4 Antibody [NB100-57521] - Whole cell lysate from HeLa (5, 15 and 50 ug, HEK293T (T; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody used at 0.04 ug/ml. Detection: Chemiluminescence with exposure times of 3seconds.")
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: CHD4 Antibody [NB100-57521] - Detection of human CHD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CHD4 antibody NB100-57521 used for IP at 6 ug per reaction. CHD4 was also immunoprecipitated by rabbit anti-CHD4 antibodies (NB100-57519, NB100-57520), and rabbit anti-CHD4 recombinant monoclonal antibody [BLR066G] (NBP2-77358). Detection: Chemiluminescence with an exposure time of 1 second.](http://images.novusbio.com/fullsize/CHD4-Antibody-NB100-57521-img0003.jpg)