| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84748-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44026 | |||
| BSA Free | NBP2-52910-25ul | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 647 | DDX0141A647-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65729SS | |||
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Immunocytochemistry-Immunofluorescence-DDX0140P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - IF staining of hCD40L-transfected L cells with LL48 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - FACS staining of hCD40L-transfected L cells with LL48 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Flow-Cytometry-DDX0140P-100-img0002.jpg)
![Neutralization: CD40 Ligand/TNFSF5 Antibody (LL48) [DDX0140P-100] - Inhibition of IL12 production by CD40L-stimulated human monocyte-derived DCs by LL48 clone (Stax AM et al, 2008, J ImmunolMethods)](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL48-Neutralization-DDX0140P-100-img0003.jpg)
![Immunohistochemistry-Paraffin: FCER1G Antibody [NBP2-16456] - Immunohistochemical analysis of paraffin-embedded U873 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/FCER1G-Antibody-Immunohistochemistry-Paraffin-NBP2-16456-img0002.jpg "Immunohistochemistry-Paraffin: FCER1G Antibody [NBP2-16456] - Immunohistochemical analysis of paraffin-embedded U873 xenograft, using antibody at 1:100 dilution.")
![ICC/IF-FCER1G Antibody [NBP2-16456] -HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FCER1G stained by FCER1G antibody [N2C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/antibody/nbp2-16456_rabbit-polyclonal-fcer1g-antibody-immunocytochemistry-immunofluorescence-2572023123444..jpg)
![Western Blot: FCER1G Antibody [NBP2-16456] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16456_rabbit-polyclonal-fcer1g-antibody-western-blot-2572023123637..jpg)
![Western Blot: FCER1G Antibody [NBP2-16456] - Non-transfected - and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3]diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp2-16456_rabbit-polyclonal-fcer1g-antibody-western-blot-2572023123822..jpg)
![FCER1G Antibody [NBP2-16456] - THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: FCER1G stained by FCER1G antibody [N2C3] diluted at 1:500.Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/antibody/nbp2-16456_rabbit-polyclonal-fcer1g-antibody-immunocytochemistry-immunofluorescence-257202312404..jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: FCER1G Antibody [NBP2-16456] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with FCER1G antibody [N2C3] diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16456_rabbit-polyclonal-fcer1g-antibody-1610202419414525.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-84748] - Staining of human urinary bladder.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84748-img0013.jpg "Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-84748] - Staining of human urinary bladder.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-84748] - Staining of human kidney, liver, testis and urinary bladder using Anti-GSTP1 antibody NBP1-84748 (A) shows similar protein distribution across tissues to independent antibody NBP1-84747 (B).](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84748-img0008.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-84748] - Staining of human testis.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84748-img0010.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-84748] - Staining of human liver.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84748-img0011.jpg)
![Immunohistochemistry-Paraffin: Glutathione S-Transferase pi 1/GSTP1 Antibody [NBP1-84748] - Staining of human kidney.](http://images.novusbio.com/fullsize/Glutathione-S-Transferase-pi-1-GSTP1-Antibody-Immunohistochemistry-Paraffin-NBP1-84748-img0012.jpg)
![Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Western-Blot-NBP1-91770-img0007.jpg "Western Blot: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Analysis in control (vector only transfected HEK293T lysate) and cD40LG over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells.")
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human rectum shows strong cytoplasmic positivity in leukocytes.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0011.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human cerebral cortex shows no positivity in neurons as expected.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0008.jpg)
![Immunohistochemistry-Paraffin: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human lymph node shows strong cytoplasmic positivity in leukocytes.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-Paraffin-NBP1-91770-img0009.jpg)
![Immunohistochemistry: CD40 Ligand/TNFSF5 Antibody [NBP1-91770] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-Immunohistochemistry-NBP1-91770-img0010.jpg)
![Western Blot: CTCF Antibody (CL0305) [NBP2-52910] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Western-Blot-NBP2-52910-img0007.jpg "Western Blot: CTCF Antibody (CL0305) [NBP2-52910] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG")
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (CL0305) [NBP2-52910] - Staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunocytochemistry-Immunofluorescence-NBP2-52910-img0012.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0305) [NBP2-52910] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunohistochemistry-Paraffin-NBP2-52910-img0011.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0305) [NBP2-52910] - Staining of human endometrium shows moderate nuclear positivity in glandular and stromal cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunohistochemistry-Paraffin-NBP2-52910-img0008.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0305) [NBP2-52910] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubuli and glomeruli.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunohistochemistry-Paraffin-NBP2-52910-img0009.jpg)
![Immunohistochemistry-Paraffin: CTCF Antibody (CL0305) [NBP2-52910] - Staining of human small intestine shows strong nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/CTCF-Antibody-CL0305-Immunohistochemistry-Paraffin-NBP2-52910-img0010.jpg)
![Western Blot: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 by WB of CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (NBP2-76422). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower panel: Recombinant monoclonal antibodies to CD3e (NBP2-76424) and CD4 (NBP2-76423). A band appears at an observed molecular weight of approximately 32 kDa for the CD8 analysis.](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Western-Blot-NBP2-76422-img0005.jpg "Western Blot: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 by WB of CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (NBP2-76422). Secondary: HRP-conjugated goat anti-rabbit IgG. Lower panel: Recombinant monoclonal antibodies to CD3e (NBP2-76424) and CD4 (NBP2-76423). A band appears at an observed molecular weight of approximately 32 kDa for the CD8 analysis.")
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422] - Multiplex immunofluorescence analysis using rabbit anti-CD3E recombinant monoclonal [BL-298-5D12] , rabbit anti-CD8 alpha recombinant monoclonal [BLR044F], rabbit anti-PD-L1 recombinant monoclonal [BLR020E]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™ 520, 620, and 690. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Immunocytochemistry-Immunofluorescence-NBP2-76422-img0011.jpg)
![Immunohistochemistry-Paraffin: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 alpha in FFPE tonsil by IHC. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] (NBP2-76422). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Immunohistochemistry-NBP2-76422-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 alpha (red) in FFPE melanoma by IHC-IF. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F] NBP2-76422. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG.](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Immunocytochemistry-Immunofluorescence-NBP2-76422-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CD8 Antibody (BLR044F) [NBP2-76422] - Detection of human CD8 alpha (red) in FFPE breast carcinoma by IHC-IF. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal [BLR044F]. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).](http://images.novusbio.com/fullsize/CD8-Antibody-BLR044F-Immunocytochemistry-Immunofluorescence-NBP2-76422-img0003.jpg)
![Western Blot: GSTP1 Antibody [NBP2-16757] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/GSTP1-Antibody-Western-Blot-NBP2-16757-img0003.jpg "Western Blot: GSTP1 Antibody [NBP2-16757] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 12% SDS PAGE gel, diluted at 1:1000.")
![Western Blot: GSTP1 Antibody [NBP2-16757] - 50 ug Rat brain lysate/extract 12 % SDS-PAGE GSTP1 antibody dilution: 1:1000.](http://images.novusbio.com/fullsize/GSTP1-Antibody-Western-Blot-NBP2-16757-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Immunocytochemistry-Immunofluorescence-DDX0141P-100-img0005.jpg "Immunocytochemistry/Immunofluorescence: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - IF staining of hCD40L-transfected cellswith LL2 clone")
![Flow Cytometry: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - FACS staining of hCD40L-transfected Lcellswith LL2 clone](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Flow-Cytometry-DDX0141P-100-img0002.jpg)
![Blocking/Neutralizing: CD40 Ligand/TNFSF5 Antibody (LL2) [DDX0141P-100] - LL2 inhibitsthe stimulatory effect of the CD40L-transfected L cells on synovial fibroblasts Rissoan et al, Clin Exp Immunol. 1996, 106(3):481-90.](http://images.novusbio.com/fullsize/CD40-Ligand-TNFSF5-Antibody-LL2-Blocking-Neutralizing-DDX0141P-100-img0003.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0005.jpg "<b>Independent Antibodies Validation. </b>Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Mouse tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membranes were blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000 and competitor's antibody diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0003.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0001.jpg)
![Western Blot: IL-17/IL-17A Antibody [NBP3-13294] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with IL-17/IL-17A antibody (NBP3-13294) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Western-Blot-NBP3-13294-img0004.jpg)
![Immunohistochemistry-Paraffin: IL-17/IL-17A Antibody [NBP3-13294] - Human colon cancer. IL-17/IL-17A stained by IL-17/IL-17A antibody (NBP3-13294) diluted at 1:2000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/IL-17-IL-17A-Antibody-Immunohistochemistry-Paraffin-NBP3-13294-img0002.jpg)
![Dual RNAscope ISH-IHC: CD8 Antibody (4B11) [NB100-65729] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD8 mRNA (ACD RNAScope probe, catalog # 560391; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NB100-65729) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).](http://images.novusbio.com/fullsize/CD8-Antibody-4B11-Dual-RNAscope-ISH-IHC-NB100-65729-img0003.jpg "<b>Orthogonal Strategies Validation. </b>Dual RNAscope ISH-IHC: CD8 Antibody (4B11) [NB100-65729] - Formalin-fixed paraffin-embedded tissue sections of human tonsil were probed for CD8 mRNA (ACD RNAScope probe, catalog # 560391; Fast Red chromogen, ACD catalog # 322500) followed by immunohistochemistry using mouse monoclonal (Novus catalog # NB100-65729) at 5ug/mL for 1 hour at room temperature and detection with anti-mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems Catalog # VC001) and green HRP chromogen (ACD Catalog # 322500).")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD8 Antibody (4B11) [NB100-65729] - Analysis of formalin fixed, paraffin-embedded human tonsil with CD8 Antibody (4B11). Magnification: 40X.](http://images.novusbio.com/fullsize/CD8-alpha-Antibody-4B11-Immunohistochemistry-Paraffin-NB100-65729-img0002.jpg)
![Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Western-Blot-NBP3-15256-img0004.jpg "Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded mouse spinal cord using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0003.jpg)
![Western Blot: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Western blot analysis of extracts of various cell lines, using CaM Kinase II Rabbit mAb (NBP3-15256) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Western-Blot-NBP3-15256-img0005.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded rat brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0001.jpg)
![Immunohistochemistry-Paraffin: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry of paraffin-embedded human brain using CaM Kinase II Rabbit mAb (NBP3-15256) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/CaM-Kinase-II-Antibody-9P2E2-Immunohistochemistry-Paraffin-NBP3-15256-img0002.jpg)
![Immunohistochemistry: CaM Kinase II Antibody (9P2E2) [NBP3-15256] - Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CaM Kinase II Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-15256_rabbit-cam-kinase-ii-mab-9p2e2-72202512443379.jpg)
![Western Blot: CTCF Antibody (4W9B0) [NBP3-15795] - Western blot analysis of extracts of various cell lines, using CTCF antibody (NBP3-15795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Western-Blot-NBP3-15795-img0003.jpg "Western Blot: CTCF Antibody (4W9B0) [NBP3-15795] - Western blot analysis of extracts of various cell lines, using CTCF antibody (NBP3-15795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795] - Immunofluorescence analysis of NIH-3T3 cells using CTCF antibody (NBP3-15795). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunocytochemistry-Immunofluorescence-NBP3-15795-img0002.jpg)
![Chromatin Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody (NBP3-15795) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Chromatin-Immunoprecipitation-NBP3-15795-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795] - Immunofluorescence analysis of C6 cells using CTCF antibody (NBP3-15795). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunocytochemistry-Immunofluorescence-NBP3-15795-img0001.jpg)
![Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CTCF antibody (NBP3-15795). Western blot was performed from the immunoprecipitate using CTCF antibody (NBP3-15795) at a dilition of 1:1000.](http://images.novusbio.com/fullsize/CTCF-Antibody-4W9B0-Immunoprecipitation-NBP3-15795-img0004.jpg)