| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91782-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-44114 | |||
| BSA Free | NBP2-59036-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76799-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84561-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89068-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-91995-0.02ml | |||
![Western Blot: ACE-2 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72117] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACE2.](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Azide-and-BSA-Free-Western-Blot-NBP2-72117-img0001.jpg "Western Blot: ACE-2 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72117] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACE2.")
![Immunohistochemistry-Paraffin: ACE-2 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72117] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ACE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min.](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72117-img0005.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72117] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72117-img0002.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72117] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-ACE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72117-img0003.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (OTI4D2) - Azide and BSA Free [NBP2-72117] - staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ACE2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min](http://images.novusbio.com/fullsize/ACE-2-Antibody-OTI4D2-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-72117-img0004.jpg)
![Western Blot: CHMP4B Antibody [NBP1-91782] - Analysis in control (vector only transfected HEK293T lysate) and CHMP4B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP1-91782-img0013.jpg "Western Blot: CHMP4B Antibody [NBP1-91782] - Analysis in control (vector only transfected HEK293T lysate) and CHMP4B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP1-91782-img0012.jpg)
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human liver shows weak granular cytoplasmic positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP1-91782-img0008.jpg)
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP1-91782] - Staining of human small intestine shows moderate to strong granular cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP1-91782-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IKK gamma stained by IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-212024939145.jpg "Immunocytochemistry/Immunofluorescence: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IKK gamma stained by IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.")
![Immunohistochemistry-Paraffin: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - IKK gamma antibody [HL1577] detects IKK gamma protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. IKK gamma stained by IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-212024943285.jpg)
![Western Blot: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - Whole cell extract (10 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-2120249432859.jpg)
![Western Blot: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-212024941426.jpg)
![Western Blot: IKK gamma Antibody (HL1577) - Azide and BSA Free [NBP3-25521] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with IKK gamma antibody [HL1577] (NBP3-25521) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25521_rabbit-ikk-gamma-mab-hl1577-azide-and-bsa-free-2120249393559.jpg)
![Western Blot: ACE-2 Antibody (CL4013) [NBP2-59036] - Analysis in human kidney tissue.](http://images.novusbio.com/fullsize/ACE-2-Antibody-CL4013-Western-Blot-NBP2-59036-img0005.jpg "Western Blot: ACE-2 Antibody (CL4013) [NBP2-59036] - Analysis in human kidney tissue.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ACE-2 Antibody (CL4013) [NBP2-59036] - Staining in human kidney and tonsil tissues using NBP2-59036 antibody. Corresponding ACE2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ACE-2-Antibody-CL4013-Immunohistochemistry-Paraffin-NBP2-59036-img0010.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (CL4013) [NBP2-59036] - Staining of human kidney shows strong positivity in apical membrane in cells in proximal tubules.](http://images.novusbio.com/fullsize/ACE-2-Antibody-CL4013-Immunohistochemistry-Paraffin-NBP2-59036-img0006.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (CL4013) [NBP2-59036] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/ACE-2-Antibody-CL4013-Immunohistochemistry-Paraffin-NBP2-59036-img0007.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (CL4013) [NBP2-59036] - Staining of human testis shows strong membranous positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/ACE-2-Antibody-CL4013-Immunohistochemistry-Paraffin-NBP2-59036-img0008.jpg)
![Immunohistochemistry-Paraffin: ACE-2 Antibody (CL4013) [NBP2-59036] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/ACE-2-Antibody-CL4013-Immunohistochemistry-Paraffin-NBP2-59036-img0009.jpg)
![Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Western-Blot-NBP2-32263-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-NBP2-32263-img0004.jpg)
![Immunohistochemistry: NAT10 Antibody [NBP2-32263] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NAT10 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunohistochemistry-NBP2-32263-img0003.jpg)
![Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by rabbit anti-NAT10 antibody BL15900. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunoprecipitation-NBP2-32263-img0002.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: NAT10 Antibody [NBP2-32263] - Detection of human NAT10 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-NAT10 antibody NBP2-32263 used for IP at 6 ug per reaction. NAT10 was also immunoprecipitated by another rabbit anti-NAT10 antibody. For blotting immunoprecipitated NAT10, NBP2-32263 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/NAT10-Antibody-Immunoprecipitation-NBP2-32263-img0005.jpg)
![Western Blot: IKK gamma Antibody [NBP1-76799] - Analysis of IKKg in HeLa whole cell lysate with anti-IKKg/NEMO using NBP1-76799 at 1 ug/ml.](http://images.novusbio.com/fullsize/IKK-gamma-Antibody-Western-Blot-NBP1-76799-img0003.jpg "Western Blot: IKK gamma Antibody [NBP1-76799] - Analysis of IKKg in HeLa whole cell lysate with anti-IKKg/NEMO using NBP1-76799 at 1 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody - BSA Free [NBP1-76799] - Immunofluorescence of IKK gamma in Hela cells with IKK gamma antibody at 20 u/mL.Red: IKK gamma Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp1-76799_rabbit-polyclonal-ikk-gamma-antibody-2711202420175134.gif)
![Immunocytochemistry/ Immunofluorescence: IKK gamma Antibody - BSA Free [NBP1-76799] - Immunocytochemistry of IKK gamma in HeLa cells with IKK gamma antibody at 5 u/mL.](http://images.novusbio.com/fullsize/nbp1-76799_rabbit-polyclonal-ikk-gamma-antibody-2711202419261832.jpg)
![Western Blot: CDR2 Antibody [NBP1-84561] - Analysis using Anti-CDR2 antibody NBP1-84561 (A) shows similar pattern to independent antibody NBP1-84562 (B).](http://images.novusbio.com/fullsize/CDR2-Antibody-Western-Blot-NBP1-84561-img0010.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CDR2 Antibody [NBP1-84561] - Analysis using Anti-CDR2 antibody NBP1-84561 (A) shows similar pattern to independent antibody NBP1-84562 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: CDR2 Antibody [NBP1-84561] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/CDR2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84561-img0009.jpg)
![Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human kidney shows strong membranous positivity in cells in tubules.](http://images.novusbio.com/fullsize/ABP1-AOC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89068-img0007.jpg "Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human kidney shows strong membranous positivity in cells in tubules.")
![Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human duodenum shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/ABP1-AOC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89068-img0005.jpg)
![Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human endometrium shows no membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/ABP1-AOC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89068-img0006.jpg)
![Immunohistochemistry-Paraffin: ABP1/AOC1 Antibody [NBP1-89068] - Staining of human placenta shows moderate membranous and cytoplasmic positivity in decidual cells.](http://images.novusbio.com/fullsize/ABP1-AOC1-Antibody-Immunohistochemistry-Paraffin-NBP1-89068-img0008.jpg)
![Western Blot: ABP1/AOC1 Antibody [NBP1-89068] - Analysis in human cell line CAPAN-2.](http://images.novusbio.com/fullsize/antibody/nbp1-89068_rabbit-polyclonal-abp1-aoc1-antibody-western-blot-98202491345..jpg)
![Western Blot: ART1 Antibody [NBP2-91995] - Analysis of extracts of various cell lines, using ART1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.](http://images.novusbio.com/fullsize/ART1-Antibody-Western-Blot-NBP2-91995-img0004.jpg "Western Blot: ART1 Antibody [NBP2-91995] - Analysis of extracts of various cell lines, using ART1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: ART1 Antibody [NBP2-91995] - Analysis of U-2 OS cells using ART1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ART1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-91995-img0002.jpg)
![Immunohistochemistry-Paraffin: ART1 Antibody [NBP2-91995] - Paraffin-embedded human lung cancer using ART1 .](http://images.novusbio.com/fullsize/ART1-Antibody-Immunohistochemistry-Paraffin-NBP2-91995-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: ART1 Antibody [NBP2-91995] - Analysis of L929 cells using ART1 at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/ART1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-91995-img0001.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0005.jpg "Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Lung Tumor. Antibody Dilution: 1ug/ml")
![Immunohistochemistry-Paraffin: CHMP4B Antibody [NBP2-87183] - Rabbit Anti-CHMP4B antibody. Formalin Fixed Paraffin Embedded Tissue: Human Kidney. Primary antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20x.](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Immunohistochemistry-Paraffin-NBP2-87183-img0001.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human Hela. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0002.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Mouse. Target Name: CHMP4B. Sample Tissue: Mouse Skeletal Muscle. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0003.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target: CHMP4B. Positive control (+): MCF7 (N10). Negative control (-): Human liver (LI). Antibody concentration: 1ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0004.jpg)
![Western Blot: CHMP4B Antibody [NBP2-87183] - Host: Rabbit. Target Name: CHMP4B. Sample Tissue: Human HepG2 Whole Cell. Antibody Dilution: 3ug/ml](http://images.novusbio.com/fullsize/CHMP4B-Antibody-Western-Blot-NBP2-87183-img0006.jpg)
![Western Blot: TNFAIP1 Antibody [NBP2-82039] - Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 ug/ml.](http://images.novusbio.com/fullsize/TNFAIP1-Antibody-Western-Blot-NBP2-82039-img0001.jpg "Western Blot: TNFAIP1 Antibody [NBP2-82039] - Western blot analysis of TNFAIP1 in mouse brain tissue lysate with TNFAIP1 antibody at 1 ug/ml.")
![Immunohistochemistry: TNFAIP1 Antibody - BSA Free [NBP2-82039] - Immunohistochemistry of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 10 ug/ml.](http://images.novusbio.com/fullsize/nbp2-82039_rabbit-polyclonal-tnfaip1-antibody-271120241935386.jpg)
![Immunocytochemistry/ Immunofluorescence: TNFAIP1 Antibody - BSA Free [NBP2-82039] - Immunofluorescence of TNFAIP1 in mouse brain tissue with TNFAIP1 antibody at 20 ug/ml.Red: TNFAIP1 Antibody Blue: DAPI staining](http://images.novusbio.com/fullsize/nbp2-82039_rabbit-polyclonal-tnfaip1-antibody-2711202420285833.gif)
![Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-288202412442915.jpg "Western Blot: Rac1,2,3 Antibody [NBP3-32887] - Western blot analysis of Rac1,2,3 on different lysates with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/500 dilution. Lane 1: Rat brain tissue lysate Lane 2: Human brain tissue lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 21 kDa Observed band size: 21 kDa Exposure time: 5 minutes; 15% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32887) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: Rac1,2,3 Antibody [NBP3-32887] - Immunohistochemical analysis of paraffin-embedded rat skin tissue with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32887) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29820241420957.jpg)
![Immunocytochemistry/ Immunofluorescence: Rac1,2,3 Antibody [NBP3-32887] - Immunocytochemistry analysis of SH-SY5Y cells labeling Rac1,2,3 with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Rac1,2,3 antibody (NBP3-32887) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32887_rabbit-rac1-2-3-pab-29202410234241.jpg)